Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,196,101 G→T 16.1% intergenic (‑2/+231) thiC ← / ← rsd phosphomethylpyrimidine synthase/stationary phase protein, binds sigma 70 RNA polymerase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,196,1010GT16.1% 470.6 / 72.5 172intergenic (‑2/+231)thiC/rsdphosphomethylpyrimidine synthase/stationary phase protein, binds sigma 70 RNA polymerase subunit
Reads supporting (aligned to +/- strand):  ref base G (72/72);  new base T (13/15);  total (85/87)
Fisher's exact test for biased strand distribution p-value = 8.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

Only 100 of 174 total aligned reads displayed.

ATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCC  >  NC_000913/4196002‑4196200
                                                                                                   |                                                                                                   
aTGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAg                                                                                                     >  1:3612153/1‑100 (MQ=255)
      tttGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCAtt                                                                                               <  2:3757493/100‑1 (MQ=255)
       ttGAGTTCGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATATCTCATTc                                                                                              <  1:354422/100‑1 (MQ=255)
        tGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTcc                                                                                             <  2:1500626/100‑1 (MQ=255)
        tGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTcc                                                                                             <  2:3835085/100‑1 (MQ=255)
        tGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTcc                                                                                             <  2:627706/100‑1 (MQ=255)
        tGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCACGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATATCTCATTcc                                                                                             >  2:1248205/1‑100 (MQ=255)
         gAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCa                                                                                            >  1:2758367/1‑100 (MQ=255)
         cgatctggAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCa                                                                                            <  2:11138/94‑1 (MQ=255)
          aGTTGGGAAAGGCGGTGCCTTCCAGGGTGTGGAGAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCaa                                                                                           >  1:2531029/1‑100 (MQ=255)
           gTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCaaa                                                                                          >  2:3533887/1‑100 (MQ=255)
           gTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCaaa                                                                                          >  2:2328020/1‑100 (MQ=255)
            ttGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCaaaa                                                                                         <  1:2303781/100‑1 (MQ=255)
             tGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCaaaaa                                                                                        >  2:302/1‑100 (MQ=255)
             tGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCaaaaa                                                                                        <  2:2442255/100‑1 (MQ=255)
              gggAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAg                                                                                       <  1:2475090/100‑1 (MQ=255)
               ggAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATATCTCATTCCAAAAAGt                                                                                      <  2:1708886/100‑1 (MQ=255)
               ggAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCaagatcg                                                                                      >  1:11138/1‑95 (MQ=255)
               ggAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGt                                                                                      >  1:3385589/1‑100 (MQ=255)
               ggAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGt                                                                                      <  1:2007626/100‑1 (MQ=255)
               ggAAAGGCGGTGCCTTCCAGAGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGt                                                                                      >  2:3623409/1‑100 (MQ=255)
                gAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGtt                                                                                     >  1:3474755/1‑100 (MQ=255)
                  aaGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTaa                                                                                   <  1:1521047/100‑1 (MQ=255)
                  aaGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGAGGCGGGTAAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTa                                                                                    <  2:538439/99‑1 (MQ=255)
                   aGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAg                                                                                  <  1:3673067/100‑1 (MQ=255)
                   aGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAg                                                                                  <  1:3113469/100‑1 (MQ=255)
                    ggCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAgg                                                                                 <  2:3752449/100‑1 (MQ=255)
                       ggTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACg                                                                              <  1:3140298/100‑1 (MQ=255)
                        gTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATATCTCATTCCAAAAAGTTAAGGACGt                                                                             >  2:861381/1‑100 (MQ=255)
                         tGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTg                                                                            <  2:231801/100‑1 (MQ=255)
                            cTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCt                                                                         >  2:453058/1‑100 (MQ=255)
                              tCCAGGGTGTCGATAAAATGTTGGGTCAGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATCCCAAAAAGTTAAGGACGTg                                                                            <  1:1024345/95‑1 (MQ=255)
                              tCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTg                                                                       <  2:1134650/100‑1 (MQ=255)
                                cAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTc                                                                     <  1:2393909/100‑1 (MQ=255)
                                 aGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCa                                                                    <  2:1172916/100‑1 (MQ=255)
                                  gggTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAg                                                                   <  2:2051043/100‑1 (MQ=255)
                                    gtgtCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAgac                                                                 <  1:1836435/100‑1 (MQ=255)
                                    gtgtCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAga                                                                  >  1:1818155/1‑99 (MQ=255)
                                       tCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAgacgac                                                              >  2:621805/1‑100 (MQ=255)
                                        cGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGacgacg                                                             >  2:3474402/1‑100 (MQ=255)
                                         gATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACgg                                                            <  2:3516508/100‑1 (MQ=255)
                                          aTAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGa                                                           >  1:3755275/1‑100 (MQ=255)
                                          aTAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGa                                                           <  2:1498769/100‑1 (MQ=255)
                                           tAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATATCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGAt                                                          <  1:739004/100‑1 (MQ=255)
                                           tAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGa                                                           >  1:422349/1‑99 (MQ=255)
                                           tAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGAt                                                          >  1:872421/1‑100 (MQ=255)
                                            aaaaTGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATATCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATg                                                         >  2:196979/1‑100 (MQ=255)
                                             aaaTGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGa                                                        >  2:108081/1‑100 (MQ=255)
                                             aaaTGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGa                                                        >  2:2601888/1‑100 (MQ=255)
                                             aaaTGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCAAAAAAGTTAAGGACGTGGCTTGTCAgacgac                                                              >  1:718478/1‑94 (MQ=255)
                                                tGTTGGGCCCGGGCGCGTTGTTTGCGGCGAGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACAACGGCTGAAGc                                                     >  1:2237133/1‑100 (MQ=255)
                                                tGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGc                                                     <  2:956095/100‑1 (MQ=255)
                                                tGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGc                                                     >  2:1204001/1‑100 (MQ=255)
                                                tGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGc                                                     >  1:3428770/1‑100 (MQ=255)
                                                 gTTGGGCCCGGGCGCGTTGTTCGCGGCGAGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCa                                                    <  1:3623383/100‑1 (MQ=255)
                                                  ttGGTCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCAGTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACTGATGAAg                                                      <  1:1735587/97‑1 (MQ=255)
                                                    gggCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAaga                                                 <  1:319685/100‑1 (MQ=255)
                                                     ggCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTATCAGACGACGGATGAAGCAagag                                                >  2:2743487/1‑100 (MQ=255)
                                                      gCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAgaga                                               >  1:524696/1‑100 (MQ=255)
                                                      gCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAgaga                                               <  2:2122921/100‑1 (MQ=255)
                                                      gCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAgaga                                               >  2:423628/1‑100 (MQ=255)
                                                       cccGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGAc                                              <  1:2877479/100‑1 (MQ=255)
                                                               cgTTGTTCGCGGCGGGTCAGTTTTGTTGCAGATATATCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATcgccg                                      >  2:3650919/1‑100 (MQ=255)
                                                               cgTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATcgccg                                      >  1:182824/1‑100 (MQ=255)
                                                               cgTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATcgccg                                      >  2:1818369/1‑100 (MQ=255)
                                                                gttgttCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATcgccgc                                     >  2:2266669/1‑100 (MQ=255)
                                                                  tgttCGCGGCGGGTCAGTTTTGTTGCAGACATATCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAg                                   >  2:887565/1‑100 (MQ=255)
                                                                  tgttCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAg                                   <  2:3009091/100‑1 (MQ=255)
                                                                   gttCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAgg                                  >  2:2426550/1‑100 (MQ=255)
                                                                   gttCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAgg                                  >  2:387746/1‑100 (MQ=255)
                                                                   gttCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAgg                                  >  2:1214394/1‑100 (MQ=255)
                                                                    ttCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAggg                                 >  2:2583435/1‑100 (MQ=255)
                                                                      cgcgGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAATAGTTAAGGACGTGGCTTGTAAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgc                               >  1:2102150/1‑100 (MQ=255)
                                                                       gcggcgGGTCAGTTTTGTTGCAAACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGAGCGACGCAGGGgc                               >  1:2836412/1‑99 (MQ=255)
                                                                        cggcggGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgcga                             >  2:460234/1‑100 (MQ=255)
                                                                           cggGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgcgatgc                          <  2:1327679/100‑1 (MQ=255)
                                                                           cggGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgcgatgc                          <  1:1716322/100‑1 (MQ=255)
                                                                           cggGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgcgatgc                          <  1:88707/100‑1 (MQ=255)
                                                                            gggTCAGTTTTGTTGCAGACATATCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgcgatgc                          <  1:3650893/99‑1 (MQ=255)
                                                                             ggTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgcgatgcga                        >  1:1954984/1‑100 (MQ=255)
                                                                              gTCAGTTTTGTTGCAGACATATCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgcgatgcgat                       >  2:1472768/1‑100 (MQ=255)
                                                                              gTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGgcgatgcgat                       >  2:697720/1‑100 (MQ=255)
                                                                               tCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATa                      <  1:3288916/100‑1 (MQ=255)
                                                                                cAGTTTTGTTGCAGACATATCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAg                     <  2:3607071/100‑1 (MQ=255)
                                                                                 aGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGc                    <  1:3007090/100‑1 (MQ=255)
                                                                                  gTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTTAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCTATGCGATAGCa                   <  1:2028266/100‑1 (MQ=255)
                                                                                  gTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCa                   >  2:2793500/1‑100 (MQ=255)
                                                                                   ttttGTTGCAGACATATCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAg                  >  2:3184008/1‑100 (MQ=255)
                                                                                    tttGTTGCAGACATATCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGa                 <  2:788863/100‑1 (MQ=255)
                                                                                         tgCAGACATATCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAAc            >  1:3795473/1‑100 (MQ=255)
                                                                                          gCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAAct           >  1:3497645/1‑100 (MQ=255)
                                                                                            aGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAActct         <  2:863723/100‑1 (MQ=255)
                                                                                              aCATATCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTg       <  2:3816651/100‑1 (MQ=255)
                                                                                               cATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGt      >  2:1359386/1‑100 (MQ=255)
                                                                                                aTAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGtt     <  2:1482188/100‑1 (MQ=255)
                                                                                                 tAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTc    <  1:679570/100‑1 (MQ=255)
                                                                                                 tAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTc    >  2:576558/1‑100 (MQ=255)
                                                                                                 tAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGAGAGCAGATTAACTCTTGtt     <  1:2830104/99‑1 (MQ=255)
                                                                                                  aGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTcc   <  1:3274055/100‑1 (MQ=255)
                                                                                                   gCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTccc  <  1:2429475/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
ATGCGTTTTGAGTTGGGAAAGGCGGTGCCTTCCAGGGTGTCGATAAAATGTTGGGCCCGGGCGCGTTGTTCGCGGCGGGTCAGTTTTGTTGCAGACATAGCTCATTCCAAAAAGTTAAGGACGTGGCTTGTCAGACGACGGATGAAGCAAGAGACGATCGCCGCAGGGGCGATGCGATAGCAGATTAACTCTTGTTCCC  >  NC_000913/4196002‑4196200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: