BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,400,313	A→C	100%	D9A (GAT→GCT)	hfq →	global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,400,313	0	A	C	100.0%	542.9 / NA	153	D9A (GAT→GCT)	hfq	global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (75/78); total (75/78)

Only 100 of 153 total aligned reads displayed.

AGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATC  >  NC_000913/4400216‑4400408

 gaCGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTcc                                                                                              >  2:3026018/1‑100 (MQ=255)

 gaCGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTcc                                                                                              <  2:1433400/100‑1 (MQ=255)

    gTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGtt                                                                                           <  1:1968576/100‑1 (MQ=255)

    gTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGACAATCTTTACAAGCTCCg                                                                                             >  1:3608492/1‑98 (MQ=255)

      aTCGTGCGCAATTTTTTCAGAAGCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTcc                                                                                         <  1:1047782/100‑1 (MQ=255)

       tCGTGCACAATTTTTTCAGAATCGAGAGGTTCCAAGTAAAAATAAGCATAGAAGGAAAAGAGAGAAGGGCTAAGGGGCAATCTTTACAAGCTCAGTTcc                                                                                         <  1:143548/99‑1 (MQ=255)

         gTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGa                                                                                      >  2:785488/1‑100 (MQ=255)

         gTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGa                                                                                      <  2:2794432/100‑1 (MQ=255)

           gcgcAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAAc                                                                                    >  1:433112/1‑100 (MQ=255)

            cgcAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACg                                                                                   <  2:2505938/100‑1 (MQ=255)

             gcAATTTTTTCAGAATCGAAAGGTTCAAAGTATAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCGTTACAAGCTCCGTTCCTGAAc                                                                                    <  1:873630/98‑1 (MQ=255)

             gcAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAAc                                                                                    <  1:935225/98‑1 (MQ=255)

             gcAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGc                                                                                  >  2:2276649/1‑100 (MQ=255)

              cAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCa                                                                                 >  2:1655184/1‑100 (MQ=255)

               aaTTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCa                                                                                 >  1:1206515/1‑99 (MQ=255)

               aaTTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCAc                                                                                >  2:3297377/1‑100 (MQ=255)

                aTTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACt                                                                               >  2:2760839/1‑100 (MQ=255)

                aTTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACACACt                                                                               >  2:3380131/1‑100 (MQ=255)

                  tttttCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGc                                                                             <  1:1881898/100‑1 (MQ=255)

                    tttCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCCAAGGGGCACTCTTCACAAGCTCCGCTCCTGAACGCACTGc                                                                             >  1:3802717/1‑98 (MQ=255)

                     ttCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTc                                                                          <  1:3555920/100‑1 (MQ=255)

                      tCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCg                                                                         <  2:1950535/100‑1 (MQ=255)

                       cAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCgg                                                                        <  1:1750115/100‑1 (MQ=255)

                       cAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCgg                                                                        <  2:3298202/100‑1 (MQ=255)

                       cAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACCGCGTCgg                                                                        <  1:1467627/100‑1 (MQ=255)

                        acaaTCAAAAGGTTCAAAGTACAAATAAGCATATAAGGAACAGGGAAAATGGCTAAGGAGCAATCTTTACAGGCGCAGTTCCTGAACGCACTGCGTCg                                                                         <  1:29945/96‑1 (MQ=255)

                         gAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGa                                                                      <  2:1500616/100‑1 (MQ=255)

                          tcttccgatcttCAAAGTACAAATAAGCATATAAGGTAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGaa                                                                     <  2:2703123/90‑1 (MQ=255)

                           aTCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCCG‑ACGCAGTGCGttcaggaac                                                                   <  2:3054559/100‑9 (MQ=37)

                           aTCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCCG‑ACGCAGTGCGttcaggaac                                                                   <  1:2905546/100‑9 (MQ=37)

                            tCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGTGAACg                                                                   >  1:3589643/1‑100 (MQ=255)

                            tCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACg                                                                   <  2:676069/100‑1 (MQ=255)

                            tCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACg                                                                   <  1:2891710/100‑1 (MQ=255)

                             cGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACgt                                                                  <  2:2305379/100‑1 (MQ=255)

                             cGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCCG‑ACGCAGTGCGttcaggaacg                                                                  <  1:1153044/99‑10 (MQ=37)

                              gAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACgtg                                                                 <  1:3049380/100‑1 (MQ=255)

                              gAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACgtg                                                                 >  1:2816765/1‑100 (MQ=255)

                              gAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACgtg                                                                 >  1:1169937/1‑100 (MQ=255)

                                   gTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCa                                                            <  2:3015148/100‑1 (MQ=255)

                                     tCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGt                                                          <  2:3681785/100‑1 (MQ=255)

                                      cAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGtt                                                         >  2:593396/1‑100 (MQ=255)

                                      cAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGtt                                                         >  2:3377154/1‑100 (MQ=255)

                                        aaGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTc                                                       >  2:1148575/1‑100 (MQ=255)

                                          gTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCta                                                     <  1:2914394/100‑1 (MQ=255)

                                          gTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCta                                                     <  1:904382/100‑1 (MQ=255)

                                          gTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCta                                                     >  2:3089312/1‑100 (MQ=255)

                                          gTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCta                                                     >  1:3352102/1‑100 (MQ=255)

                                          gTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCta                                                     >  1:2961428/1‑100 (MQ=255)

                                           tACAAATAAGCATATAAGGACAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCt                                                      >  1:2347281/1‑98 (MQ=255)

                                           tACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCtat                                                    >  1:2084418/1‑100 (MQ=255)

                                           tACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCtat                                                    <  2:546979/100‑1 (MQ=255)

                                            aCAAATAAGTATATAAGGAAAAGAGAAAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCGGACCACAATGCATCGGGCACGTGTTCCAGTTTCta                                                     >  1:938332/1‑98 (MQ=255)

                                             cAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCtattt                                                  >  1:137924/1‑100 (MQ=255)

                                              aaaTAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCtattta                                                 >  1:1012826/1‑100 (MQ=255)

                                              aaaTAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCtattta                                                 <  2:3680758/100‑1 (MQ=255)

                                                 tAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTatttattt                                              <  2:1670971/100‑1 (MQ=255)

                                                  aaGCATATAAGG‑AAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTgg                                            >  1:3435760/1‑100 (MQ=255)

                                                    gCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGt                                           <  2:2596982/100‑1 (MQ=255)

                                                    gCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGt                                           <  2:2974263/100‑1 (MQ=255)

                                                     cATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTg                                          <  2:17360/100‑1 (MQ=255)

                                                      atatAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTACGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGa                                         >  1:2547299/1‑100 (MQ=255)

                                                         taAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATg                                      >  1:2417187/1‑100 (MQ=255)

                                                         taAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATg                                      >  1:1423471/1‑100 (MQ=255)

                                                          aaGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATgn                                     <  2:107238/100‑2 (MQ=255)

                                                          aaGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATgg                                     >  1:1356076/1‑100 (MQ=255)

                                                           aGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGt                                    <  2:499884/100‑1 (MQ=255)

                                                           aGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGt                                    <  2:500145/100‑1 (MQ=255)

                                                             gAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTAt                                  <  1:1655170/100‑1 (MQ=255)

                                                              aaaaGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTAtt                                 >  2:2443378/1‑100 (MQ=255)

                                                               aaaGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTa                                <  1:3277118/100‑1 (MQ=255)

                                                                aaGAGAGAATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTaa                               >  2:3852946/1‑100 (MQ=255)

                                                                 agagagAATGGCT‑AGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGc                             >  1:2152797/1‑100 (MQ=255)

                                                                  gagagaATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGc                             >  2:2660799/1‑100 (MQ=255)

                                                                  gagagaATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGc                             <  1:2091882/100‑1 (MQ=255)

                                                                  gagagaATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGc                             >  1:3330633/1‑100 (MQ=255)

                                                                  gagagaATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACCGCGTCGGGAACGTGTTCCAGATTCTATTTATTTGGTGAATGGTATTCAg                              >  1:1196754/1‑99 (MQ=255)

                                                                   agagaATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCt                            >  2:2786444/1‑100 (MQ=255)

                                                                    gagaATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTg                           <  1:3535975/100‑1 (MQ=255)

                                                                      gaATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCa                         >  1:878380/1‑100 (MQ=255)

                                                                      gaATGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCa                         >  1:3450722/1‑100 (MQ=255)

                                                                      gaATGGATAAGGGACAATCTGTACTAGCGCCGTTCCTGAACGCACTGCGTCGAGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCttc                          <  1:2158483/99‑3 (MQ=255)

                                                                        aTGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGTACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAg                       <  2:1689293/100‑1 (MQ=255)

                                                                        aTGGCCAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGGACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAg                       <  1:899433/100‑1 (MQ=255)

                                                                         tGGCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAgg                      <  2:435301/100‑1 (MQ=255)

                                                                          tgCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAggg                     <  1:708009/99‑1 (MQ=255)

                                                                          ggCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAgg                      <  2:1344683/99‑1 (MQ=255)

                                                                           gCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAggga                    >  2:708016/1‑99 (MQ=255)

                                                                           gCTAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGc                    >  1:1326795/1‑100 (MQ=255)

                                                                             tAAGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCaa                  >  2:2052907/1‑100 (MQ=255)

                                                                              aaGGGGCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCaaa                 >  2:1689139/1‑100 (MQ=255)

                                                                                ggggCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATc               >  1:2505259/1‑100 (MQ=255)

                                                                                  ggCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCg              >  1:1748572/1‑99 (MQ=255)

                                                                                  ggCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGa             >  1:3250017/1‑100 (MQ=255)

                                                                                   gCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAg            >  1:1238604/1‑100 (MQ=255)

                                                                                   gCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAg            <  2:1118448/100‑1 (MQ=255)

                                                                                   gCAATCTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAg            <  1:593388/100‑1 (MQ=255)

                                                                                        ntttACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTCCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCttt       >  1:465306/2‑100 (MQ=255)

                                                                                        cTTTACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGTTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCttt       <  1:2067467/100‑1 (MQ=255)

                                                                                          ttACAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTg     <  1:3089289/100‑1 (MQ=255)

                                                                                             cAAGCTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATc  >  1:2370031/1‑100 (MQ=255)

AGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATC  >  NC_000913/4400216‑4400408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`