BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	263,172	A→C	100%	pseudogene (41/63 nt)	ykfN ←	pseudogene, YdiA family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	263,172	0	A	C	100.0%	687.8 / ‑6.7	191	pseudogene (41/63 nt)	ykfN	pseudogene, YdiA family
Reads supporting (aligned to +/- strand): ref base A (0/1); major base C (105/84); minor base T (0/1); total (105/86)

Only 100 of 191 total aligned reads displayed.

GCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTAGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACG  >  NC_000913/263076‑263268

gCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGCACAAATTCGCt                                                                                               >  1:3305566/1‑100 (MQ=255)

gCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCt                                                                                               <  1:1273339/100‑1 (MQ=255)

gCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCt                                                                                               >  2:2365358/1‑100 (MQ=255)

   tCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCAt                                                                                            >  1:3000607/1‑100 (MQ=255)

    cGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCAtt                                                                                           <  1:767323/100‑1 (MQ=255)

    cGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGCTCAATAAAGCTCGAACAAATTCGCTCAtt                                                                                           <  1:2895178/100‑1 (MQ=255)

      cTCAATATCCAGCCTTGGCTGCAGGTTTAGTTTTACACCACGCCTACATACAATGTATTGCCAATGTGTTTAATACAGCTCGAACAAATTTGCTCa                                                                                             <  1:1322174/96‑1 (MQ=255)

         aCTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGAt                                                                                      >  1:1069453/1‑100 (MQ=255)

          cTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATc                                                                                     <  2:1784891/100‑1 (MQ=255)

           tataCATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCg                                                                                    <  2:2682158/100‑1 (MQ=255)

            ataCATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCgg                                                                                   <  2:3000629/100‑1 (MQ=255)

            ataCATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCgg                                                                                   >  1:2443849/1‑100 (MQ=255)

            ataCATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCgg                                                                                   <  1:1508242/100‑1 (MQ=255)

            ataCATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCgg                                                                                   <  2:76808/100‑1 (MQ=255)

              aCATCCTTGGCTGCAGGTTTAGTTGTACACCACTTCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGtt                                                                                 <  2:1223919/100‑1 (MQ=255)

              aCATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGtt                                                                                 >  2:1706749/1‑100 (MQ=255)

                 tCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAAt                                                                              <  2:1493885/100‑1 (MQ=255)

                  ccTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATa                                                                             <  1:1026974/100‑1 (MQ=255)

                  ccTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATa                                                                             <  2:114282/100‑1 (MQ=255)

                     tGGCTGCAGGTTTAGTTGTACACCAATCCTAAAGTTAATGTGTTTGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACtt                                                                          <  1:205632/100‑1 (MQ=255)

                       gCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCa                                                                        >  2:2798675/1‑100 (MQ=255)

                       gCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCa                                                                        >  2:3251773/1‑100 (MQ=255)

                        cTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCaa                                                                       >  1:2180386/1‑100 (MQ=255)

                        cTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGATCGTACAAATTCGTTCCTTATGATCGGTTAATACtt                                                                          >  1:1788383/1‑97 (MQ=255)

                        cTGCAGGTTTAGTTGTACACCACTCCAAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCaa                                                                       >  2:2355842/1‑100 (MQ=255)

                         tGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAAc                                                                      <  2:2971485/100‑1 (MQ=255)

                          gCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACt                                                                     <  1:2286007/100‑1 (MQ=255)

                           cAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACtt                                                                    <  2:1546443/100‑1 (MQ=255)

                            aGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTc                                                                   <  1:694243/100‑1 (MQ=255)

                            aGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGACAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTc                                                                   <  2:853561/100‑1 (MQ=255)

                             ggTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCt                                                                  >  2:2337993/1‑100 (MQ=255)

                               tttAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTgg                                                                <  1:2777181/100‑1 (MQ=255)

                               tttAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTgg                                                                <  2:1977361/100‑1 (MQ=255)

                               tttAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTgg                                                                >  1:572719/1‑100 (MQ=255)

                                 tAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGtt                                                              <  2:1746543/100‑1 (MQ=255)

                                 tAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGtt                                                              >  1:1985607/1‑100 (MQ=255)

                                 tAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGtt                                                              >  1:54776/1‑100 (MQ=255)

                                     tGTACATCACTCCGAAATTCAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATGCGCTCATGATGGCCGGTTAATACTTCAACTTCTGGTTgaa                                                           <  1:1034907/99‑3 (MQ=255)

                                     tGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCAt                                                          <  1:827636/100‑1 (MQ=255)

                                     tGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCAt                                                          >  1:323715/1‑100 (MQ=255)

                                        acacCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGAt                                                       <  1:2355825/100‑1 (MQ=255)

                                         caccacTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGAtt                                                      >  2:285540/1‑100 (MQ=255)

                                         caccacTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGAtt                                                      >  1:3835167/1‑100 (MQ=255)

                                         caccacTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGAtt                                                      >  1:3038889/1‑100 (MQ=255)

                                          accacTCCTAAATTTAATGTGTTGGCAATGTGTTCACTAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGAttg                                                     <  2:2711059/100‑1 (MQ=255)

                                          accacTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGAttg                                                     <  2:3180/100‑1 (MQ=255)

                                           ccacTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGAttgt                                                    >  2:146656/1‑100 (MQ=255)

                                           ccacTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGAttgt                                                    >  1:2650301/1‑100 (MQ=255)

                                                cttAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTc                                               <  1:3696859/98‑1 (MQ=255)

                                                ccTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGAttgtttaga                                               >  2:1662550/1‑97 (MQ=255)

                                                ccTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTc                                               <  2:3784223/100‑1 (MQ=255)

                                                 cTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCACCTTCTGGTTGCATGATTGTTTGTcc                                              >  2:1907192/1‑100 (MQ=255)

                                                 cTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTc                                               >  1:1667665/1‑99 (MQ=255)

                                                  tAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTcag                                             >  2:3696885/1‑98 (MQ=255)

                                                  tAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCg                                             <  1:3627214/100‑1 (MQ=255)

                                                     aTTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCAGTa                                           >  1:2688372/1‑99 (MQ=255)

                                                         aaTGTGTTGGCAACGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTACTGGTTGCATGATTGTTTGTCCGCAAAAAg                                      >  1:1143933/1‑100 (MQ=255)

                                                            gtgtTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATa                                   <  1:3066915/100‑1 (MQ=255)

                                                             tgtTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATaa                                  >  1:3025108/1‑100 (MQ=255)

                                                              gtTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAAc                                 <  2:2643851/100‑1 (MQ=255)

                                                              gtTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAAc                                 >  2:1612306/1‑100 (MQ=255)

                                                               ttGGCAATGTGTTCAATAAAGCTCGAACAAATTCGGTCATTATGATCGGTTAATACTTCAACTTCTGGTTGTATGATTGTTTGTCCGTAAAAAGATAAcg                                <  1:3831004/100‑1 (MQ=255)

                                                               ttGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAAcg                                >  2:1040285/1‑100 (MQ=255)

                                                                 ggCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAAcgcg                              <  1:1573596/100‑1 (MQ=255)

                                                                 ggCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAAcgcg                              >  1:991172/1‑100 (MQ=255)

                                                                     aTGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTg                          >  1:2291195/1‑100 (MQ=255)

                                                                      tgtgTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGc                         <  1:1522668/100‑1 (MQ=255)

                                                                       gtgtTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGcc                        <  2:51692/100‑1 (MQ=255)

                                                                         gtTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGCTTGTTTGTCCGTAAAAAGATAACGCGCCTGCCgg                      >  1:255901/1‑100 (MQ=255)

                                                                         gtTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCgg                      >  1:3307470/1‑100 (MQ=255)

                                                                         gtTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCgg                      >  2:307751/1‑100 (MQ=255)

                                                                         gtTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCgg                      >  2:1127727/1‑100 (MQ=255)

                                                                         gtTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCgg                      >  1:2890652/1‑100 (MQ=255)

                                                                         gtTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCgg                      >  2:1274654/1‑100 (MQ=255)

                                                                         gtTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCAgg                      >  1:1419136/1‑100 (MQ=255)

                                                                         gtTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTATTTGTCCGGAAAAAGATAACGCGCCTGCCgg                      >  1:2597921/1‑100 (MQ=255)

                                                                          ttCAATAAAGCTCGAACAACTTCGCTCATTATGATCGGATAAGACTCCAACTTCTGGGTGCATGATTGTTTGTCGGCAATACGATAACGCGCCTGc                         >  1:3642440/1‑96 (MQ=255)

                                                                          ttCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCggg                     <  2:3307494/100‑1 (MQ=255)

                                                                          tcaaaTAAAGCTCAAACAAACTCGCCCATTATGATCGGTTAATACACCAACTTCTGGTTGCATGTATGTTTGTCCCTAAAAAGACAACGCGACTGCCgg                      <  1:2499758/96‑1 (MQ=255)

                                                                            cAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGgta                   >  2:3382673/1‑100 (MQ=255)

                                                                            cAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGgta                   >  2:948201/1‑100 (MQ=255)

                                                                            cAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGgta                   >  2:2744408/1‑100 (MQ=255)

                                                                            cAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGgta                   >  1:2939898/1‑100 (MQ=255)

                                                                            cAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGgta                   >  2:2465695/1‑100 (MQ=255)

                                                                             aaTAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCCACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGgtag                  <  2:2700364/100‑1 (MQ=255)

                                                                             aaTAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAGCTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGgtag                  <  1:3576332/100‑1 (MQ=255)

                                                                             aaTAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGgtag                  >  1:2566108/1‑100 (MQ=255)

                                                                              aTAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGgtagt                 <  1:1858668/100‑1 (MQ=255)

                                                                               tAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGgtagta                <  2:22430/100‑1 (MQ=255)

                                                                                aaaGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGtagtag               >  1:513876/1‑100 (MQ=255)

                                                                                 aaGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGc              >  2:2761881/1‑100 (MQ=255)

                                                                                  aGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCa             >  2:1821762/1‑100 (MQ=255)

                                                                                   gCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAg            <  2:1522976/100‑1 (MQ=255)

                                                                                    cTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAgg           <  2:3141911/100‑1 (MQ=255)

                                                                                     tCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGgc          <  2:3767837/100‑1 (MQ=255)

                                                                                      cGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGgcg         >  2:3800166/1‑100 (MQ=255)

                                                                                        aaCAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCa       <  1:3472298/100‑1 (MQ=255)

                                                                                          cAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCAtt     <  1:936684/100‑1 (MQ=255)

                                                                                           aaaTTCGCGCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTa    <  1:3799941/100‑1 (MQ=255)

                                                                                            aaTTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTAc   >  1:2108474/1‑100 (MQ=255)

GCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTAGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACG  >  NC_000913/263076‑263268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`