Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 756,929 T→A 70.5% D341E (GAT→GAA)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,9290TA70.5% 200.7 / 104.6 132D341E (GAT→GAA) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (16/23);  new base A (35/57);  total (51/81)
Fisher's exact test for biased strand distribution p-value = 8.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

Only 100 of 133 total aligned reads displayed.

GCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAA  >  NC_000913/756830‑757027
                                                                                                   |                                                                                                  
gCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAt                                                                                                    >  1:3262129/1‑100 (MQ=255)
  cacaCGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAAcc                                                                                                  <  2:1570999/100‑1 (MQ=255)
   acacGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCg                                                                                                 >  1:1821684/1‑100 (MQ=255)
   acacGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAAcc                                                                                                  <  1:3009098/99‑1 (MQ=255)
    cacGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCgg                                                                                                <  2:1093619/100‑1 (MQ=255)
     acGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGt                                                                                               <  1:704155/100‑1 (MQ=255)
      cgcgAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTg                                                                                              <  1:206368/100‑1 (MQ=255)
       gcgAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGa                                                                                             <  1:2737174/100‑1 (MQ=255)
        cgAAACTGAAACTCGATCACCTGGGTAAAGATGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGa                                                                                             <  1:1504812/99‑1 (MQ=255)
        cgAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGaa                                                                                            <  2:3405314/100‑1 (MQ=255)
         gaaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGaaa                                                                                           <  2:1497421/100‑1 (MQ=255)
         gaaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGaaa                                                                                           <  1:3459622/100‑1 (MQ=255)
          aaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTCTCCCGTACCTTCGCTCACGTCGAACCGGTGaa                                                                                            <  1:2268659/98‑1 (MQ=255)
           aactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAaga                                                                                         >  1:488713/1‑100 (MQ=255)
           aactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAaga                                                                                         <  1:1104245/100‑1 (MQ=255)
           aactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAaga                                                                                         >  2:335207/1‑100 (MQ=255)
            actgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAagag                                                                                        >  2:3714462/1‑100 (MQ=255)
             ctgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGc                                                                                       <  1:903338/100‑1 (MQ=255)
             ctgaaactCGATCACCTGGCGAAAGAAGTTGTCAAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCAGTACCTTCGCTCACGTCGATCCAGTGAAAGAGc                                                                                       >  1:2530471/1‑100 (MQ=255)
              tgaaactCGATCACCTGGGTAAAGAAGTTCTCGAAGCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGcc                                                                                      <  1:613109/100‑1 (MQ=255)
               gaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCg                                                                                     >  1:868930/1‑100 (MQ=255)
               gaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCg                                                                                     >  1:3760257/1‑100 (MQ=255)
                aaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGa                                                                                    >  1:1570495/1‑100 (MQ=255)
                   ctCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTc                                                                                 >  2:1730317/1‑100 (MQ=255)
                    tCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTcc                                                                                <  2:2570208/100‑1 (MQ=255)
                    tCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTcc                                                                                <  2:2627422/100‑1 (MQ=255)
                      gATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCgg                                                                              <  2:2114798/100‑1 (MQ=255)
                      gATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCgg                                                                              <  2:2580815/100‑1 (MQ=255)
                      gATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCgg                                                                              <  2:2156422/100‑1 (MQ=255)
                       aTCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGt                                                                             <  2:2127461/100‑1 (MQ=255)
                        tCACCTGGGTAAAGAAGTTCTCCAATCCCGTCTGCCGGGTATACTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGt                                                                             <  1:2463261/99‑1 (MQ=255)
                         cACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTa                                                                           <  2:2284669/100‑1 (MQ=255)
                             tGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATccc                                                                       <  1:2729295/100‑1 (MQ=255)
                             tGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATccc                                                                       <  1:2728668/100‑1 (MQ=255)
                             tGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATccc                                                                       <  2:3769028/100‑1 (MQ=255)
                                gTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAAc                                                                    <  2:2860720/100‑1 (MQ=255)
                                 tAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGTGCTTTCCCGTACCTTCGCTTACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAAcc                                                                   <  1:2109147/100‑1 (MQ=255)
                                 tAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAAcc                                                                   <  2:3167691/100‑1 (MQ=255)
                                 tAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTCGAGCTTTCCCGTACCTTCGCTCACGTCGACCCGGTTAAAGAGCCGATTCCGGTTATCCCAAc                                                                    <  1:742168/99‑1 (MQ=255)
                                 tAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCGGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAAcc                                                                   <  2:737665/100‑1 (MQ=255)
                                  aaaGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAAcc                                                                   >  2:2417053/1‑99 (MQ=255)
                                  aaaGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTAGAACCAGTGAAAGATCCGATTCCGGTTATCCCAACCt                                                                  >  1:2593573/1‑100 (MQ=255)
                                   aagaagTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCACACCTg                                                                 >  1:612375/1‑100 (MQ=255)
                                       agTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAc                                                             <  2:30484/100‑1 (MQ=255)
                                       agTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAc                                                             >  1:454533/1‑100 (MQ=255)
                                        gtgcgcGAAGCCCGTCTGCGGGGTATCCGGGAGCTTGCCCGGACCTTCGCTCACGACGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCa                                                              <  1:3760431/93‑1 (MQ=255)
                                        gTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACt                                                            >  2:1260765/1‑100 (MQ=255)
                                          tctcGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTAc                                                          >  2:3320050/1‑100 (MQ=255)
                                          tctcGAATCCCGTCTGCCGGGTATCCGGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTAc                                                          <  2:3220667/100‑1 (MQ=255)
                                           ctcGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACa                                                         <  1:521496/100‑1 (MQ=255)
                                           ctcGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACa                                                         <  2:475891/100‑1 (MQ=255)
                                              gAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatga                                                      >  2:889601/1‑100 (MQ=255)
                                                    cGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCg                                                <  2:2156961/100‑1 (MQ=255)
                                                     gTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCgg                                               <  2:761040/100‑1 (MQ=255)
                                                     gTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCgg                                               <  2:454539/100‑1 (MQ=255)
                                                      tCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGt                                              <  2:868937/100‑1 (MQ=255)
                                                       cTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTa                                             >  2:866970/1‑100 (MQ=255)
                                                        tGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTAt                                            >  2:477703/1‑100 (MQ=255)
                                                         gCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTAtt                                           >  2:2442282/1‑100 (MQ=255)
                                                         gCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTAtt                                           >  2:1940773/1‑100 (MQ=255)
                                                         gCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTAtt                                           >  2:3172212/1‑100 (MQ=255)
                                                          ccGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTc                                          <  1:2025052/100‑1 (MQ=255)
                                                          ccGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTc                                          <  1:925117/100‑1 (MQ=255)
                                                           cGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTcc                                         >  2:399749/1‑100 (MQ=255)
                                                            gggTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCg                                        <  2:1858312/100‑1 (MQ=255)
                                                              gTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGTCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGAc                                      <  2:3555906/100‑1 (MQ=255)
                                                              gTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGAc                                      <  2:332170/100‑1 (MQ=255)
                                                              gTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGAc                                      <  2:3106623/100‑1 (MQ=255)
                                                               tATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGAcc                                     <  2:1576903/100‑1 (MQ=255)
                                                                 tCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCaa                                   >  2:2199129/1‑100 (MQ=255)
                                                                   cTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAg                                 <  1:1124299/100‑1 (MQ=255)
                                                                   cTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAg                                 >  2:6289/1‑100 (MQ=255)
                                                                   cTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAg                                 <  1:3494903/100‑1 (MQ=255)
                                                                   cTGGAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAg                                 <  2:1284695/100‑1 (MQ=255)
                                                                     ggAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTTCGACCAAAGtt                               <  2:629261/100‑1 (MQ=255)
                                                                     ggAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGtt                               >  2:3404362/1‑100 (MQ=255)
                                                                      gAGCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTa                              <  2:826163/100‑1 (MQ=255)
                                                                        gCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTAcc                            >  1:147153/1‑100 (MQ=255)
                                                                        gCTTTCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTAcc                            >  1:1130211/1‑100 (MQ=255)
                                                                            tCCCGTACCTTCGCTCACGTTGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTc                        <  1:1262487/100‑1 (MQ=255)
                                                                            tCCCGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCTGATTCTGGTTATCCCAACCTGTAACTACATGATGGGCGGTATTCCGACAAAAGTTAc                             >  1:1832252/1‑95 (MQ=255)
                                                                             cccGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCa                       <  2:1489226/100‑1 (MQ=255)
                                                                             cccGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCa                       >  1:2321250/1‑100 (MQ=255)
                                                                              ccGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAg                      <  2:1550384/100‑1 (MQ=255)
                                                                               cGTACCTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAgg                     <  1:2562958/100‑1 (MQ=255)
                                                                                   ccTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCact                 >  2:573335/1‑100 (MQ=255)
                                                                                   ccTTCGCTCACGTCAAACCGGTGAAAGAGCCGAGTCCGGTTATCCCAACCTGTCACCACATGATGGGCGGTATTCAGACCAAAGTTACCGGTCAGGCact                 <  1:3284998/100‑1 (MQ=255)
                                                                                    cTTCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactg                <  1:3432926/100‑1 (MQ=255)
                                                                                     ttCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactga               >  2:171492/1‑100 (MQ=255)
                                                                                     ttCGCTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactga               >  2:3638217/1‑100 (MQ=255)
                                                                                         cTCACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACtgt           >  2:1811573/1‑100 (MQ=255)
                                                                                          tCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACtgtg          >  2:462999/1‑100 (MQ=255)
                                                                                           cACGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGa         >  2:3319992/1‑100 (MQ=255)
                                                                                            aCGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGa         <  1:81613/99‑1 (MQ=255)
                                                                                            aCGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCCACCAAAGTTACCGGTCAGGCACTGACTGTGaa        <  2:3654501/100‑1 (MQ=255)
                                                                                             cGTCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAAt       >  1:1447188/1‑100 (MQ=255)
                                                                                               tCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATga     <  1:598278/100‑1 (MQ=255)
                                                                                               tCGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATga     >  2:3433275/1‑100 (MQ=255)
                                                                                                cGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATgag    <  2:3834267/100‑1 (MQ=255)
                                                                                                cGAACCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATgag    <  1:3670868/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
GCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAA  >  NC_000913/756830‑757027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑