Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,177,647 C→A 15.8% L110I (CTT→ATT)  lolE → lipoprotein‑releasing system transmembrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,177,6470CA15.8% 461.8 / 66.6 166L110I (CTT→ATT) lolElipoprotein‑releasing system transmembrane protein
Reads supporting (aligned to +/- strand):  ref base C (76/63);  new base A (16/10);  total (92/74)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

Only 100 of 169 total aligned reads displayed.

AACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAG  >  NC_000913/1177548‑1177746
                                                                                                   |                                                                                                   
 accggCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATc                                                                                                     <  1:1812243/96‑1 (MQ=255)
  cTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCtt                                                                                                   >  2:1991466/1‑100 (MQ=255)
   tGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTc                                                                                                  >  1:690907/1‑100 (MQ=255)
    ggCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGTGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTcg                                                                                                 <  2:3172476/100‑1 (MQ=255)
    ggCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTcg                                                                                                 <  1:3514626/100‑1 (MQ=255)
    ggCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTcg                                                                                                 <  2:194452/100‑1 (MQ=255)
    ggCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTcg                                                                                                 <  2:2863626/100‑1 (MQ=255)
        ggAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATCGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAAGATTCgcgcc                                                                                             <  2:2705723/100‑2 (MQ=255)
         gAAGCACTGGATCACGTACAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAAGATTCgcgcca                                                                                            <  2:614963/100‑3 (MQ=255)
          aaGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAAt                                                                                           >  2:2281004/1‑100 (MQ=255)
          aaGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAAt                                                                                           >  2:368496/1‑100 (MQ=255)
          aaGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAAGATTCgcgccac                                                                                           <  1:3849470/100‑4 (MQ=255)
          aaGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAAGATTCgcgccac                                                                                           <  2:2812189/100‑4 (MQ=255)
          aaGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAAGATTCgcgccac                                                                                           <  2:615514/100‑4 (MQ=255)
          aaGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGAATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAAt                                                                                           >  2:2281213/1‑100 (MQ=255)
             cACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCa                                                                                        >  1:1220271/1‑100 (MQ=255)
              aCTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAAGATTCgcgccactttc                                                                                       <  2:1314613/100‑8 (MQ=255)
               cTGGATCTCGTGCAAAAAGTGCCGGGTAGTGCCGCTGCCGCGCCGGATATCGATTGCACCGGGCTGGTGGAGAGTTGCGCGAAGATCCgcgccactttcc                                                                                      <  1:3308842/100‑9 (MQ=255)
               cTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAgg                                                                                      >  1:490602/1‑100 (MQ=255)
               cTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAgg                                                                                      >  1:3203079/1‑100 (MQ=255)
               cTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGCTGGAAAGTGGCGCGAATCTTCGCGCAATCCAgg                                                                                      >  1:1815332/1‑100 (MQ=255)
                tGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGt                                                                                     <  1:651787/100‑1 (MQ=255)
                tGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGt                                                                                     <  2:2851532/100‑1 (MQ=255)
                   aTCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGaa                                                                                  <  2:2068491/100‑1 (MQ=255)
                   aTCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGaa                                                                                  >  2:3493165/1‑100 (MQ=255)
                      aCGTGCAAAAAGTGCCGAGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAggg                                                                               <  1:867121/100‑1 (MQ=255)
                        gTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCg                                                                             <  1:2589746/100‑1 (MQ=255)
                          gCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGtt                                                                           <  2:1122923/100‑1 (MQ=255)
                           cAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTa                                                                          >  1:2996236/1‑100 (MQ=255)
                           cAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTa                                                                          <  2:1922642/100‑1 (MQ=255)
                           cAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATACAGGTGAAGGGCGTTa                                                                          >  1:2258099/1‑100 (MQ=255)
                            aaaaaGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTaa                                                                         >  2:1052731/1‑100 (MQ=255)
                            aaaaaGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTaa                                                                         >  2:3291833/1‑100 (MQ=255)
                            aaaaaGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAACTGGCGCGAATCGTCGCGCAATCCAGGTGAAGGGCGTTaa                                                                         >  1:2459046/1‑100 (MQ=255)
                             aaaaGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTaa                                                                         >  2:3606705/1‑99 (MQ=255)
                             aaaaGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAAc                                                                        >  1:3849769/1‑100 (MQ=255)
                                aGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCg                                                                     <  2:2032715/100‑1 (MQ=255)
                                aGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACAGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCg                                                                     <  1:2705150/100‑1 (MQ=255)
                                 gTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGc                                                                    <  2:3123410/100‑1 (MQ=255)
                                  tGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCa                                                                   >  2:3734472/1‑100 (MQ=255)
                                  tGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCGTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCa                                                                   <  1:404475/100‑1 (MQ=255)
                                   ggcGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCaa                                                                  >  2:2611853/3‑100 (MQ=255)
                                   gCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCaa                                                                  >  1:487607/1‑100 (MQ=255)
                                    ccGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAAc                                                                 <  1:1418622/100‑1 (MQ=255)
                                     cGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACa                                                                >  1:1631227/1‑100 (MQ=255)
                                      gggTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAg                                                               <  2:2407618/100‑1 (MQ=255)
                                        gTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGa                                                             <  1:368490/100‑1 (MQ=255)
                                         tATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGaa                                                            <  1:1487040/100‑1 (MQ=255)
                                          aTTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCACAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAAc                                                           <  2:2209619/100‑1 (MQ=255)
                                           ttGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACa                                                          <  1:3429288/100‑1 (MQ=255)
                                            tgccgctgccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAg                                                         >  2:1161316/1‑100 (MQ=255)
                                            tgccgctgccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAg                                                         >  1:3036768/1‑100 (MQ=255)
                                            tgccgctgccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAg                                                         <  2:1681279/100‑1 (MQ=255)
                                            tgccgctgccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAg                                                         >  2:343152/1‑100 (MQ=255)
                                             gccgctgccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGc                                                        >  1:1699641/1‑100 (MQ=255)
                                              ccgctgccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCg                                                       <  2:1614892/100‑1 (MQ=255)
                                               cgctgccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGt                                                      >  1:1673400/1‑100 (MQ=255)
                                                gctgccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTc                                                     >  2:2935977/1‑100 (MQ=255)
                                                  tgccgcGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTg                                                   <  2:875195/100‑1 (MQ=255)
                                                      ctgcCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAgcg                                               <  1:1368561/98‑1 (MQ=255)
                                                       cgcCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAgcgc                                              <  1:1246032/100‑1 (MQ=255)
                                                        gcCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCa                                             >  1:2852322/1‑100 (MQ=255)
                                                        gcCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGATAACCCGCAACAGGAACAGCGTCTGAgcgag                                             >  2:1368572/1‑98 (MQ=255)
                                                          cGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCAtt                                           <  2:1815345/100‑1 (MQ=255)
                                                             atatCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTAcc                                        <  1:2551206/100‑1 (MQ=255)
                                                               atCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCt                                      >  1:1167747/1‑100 (MQ=255)
                                                                tCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTc                                     >  2:3103153/1‑100 (MQ=255)
                                                                 cAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCg                                    <  2:358764/100‑1 (MQ=255)
                                                                  aaTTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCCGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCg                                    <  1:1066387/99‑1 (MQ=255)
                                                                   aTTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGtt                                  >  2:1083670/1‑100 (MQ=255)
                                                                    tttCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGttt                                 <  2:2441846/100‑1 (MQ=255)
                                                                     ttCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTg                                <  2:2575073/100‑1 (MQ=255)
                                                                     ttCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTg                                <  1:1204962/100‑1 (MQ=255)
                                                                     ttCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTg                                >  2:3627184/1‑100 (MQ=255)
                                                                       cACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTc                              <  2:2555712/100‑1 (MQ=255)
                                                                        aCCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTcc                             >  2:1720424/1‑100 (MQ=255)
                                                                         ccGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCa                            >  2:3270751/1‑100 (MQ=255)
                                                                            ggCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAggg                         >  2:2317356/1‑100 (MQ=255)
                                                                             gCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGc                        <  1:1353695/100‑1 (MQ=255)
                                                                                ggtggAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGAt                     >  1:2480403/1‑100 (MQ=255)
                                                                                 gtggACAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGCACAGAGTCTGCGCGCCTTACCCTCGTTTCTCCAGGGc                        >  1:3106066/1‑96 (MQ=255)
                                                                                 gtggAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATg                    <  2:802188/100‑1 (MQ=255)
                                                                                   ggAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCg                  <  2:1867306/100‑1 (MQ=255)
                                                                                   ggAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCg                  >  2:1020575/1‑100 (MQ=255)
                                                                                   cctggattgcgcGAAGATTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCg                  >  1:2122222/9‑100 (MQ=255)
                                                                                   cctggattgcgcGAAGATTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCg                  >  2:375885/9‑100 (MQ=255)
                                                                                    gAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGt                 <  1:3605503/100‑1 (MQ=255)
                                                                                      ggattgcgcGAAGATTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTgg               >  2:579599/6‑100 (MQ=255)
                                                                                       gattgcgcGAAGATTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGgc              >  1:2205763/5‑100 (MQ=255)
                                                                                       gattgcgcGAAGATTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGgc              >  1:1938458/5‑100 (MQ=255)
                                                                                       gattgcgcGAAGATTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGgc              >  1:764035/5‑100 (MQ=255)
                                                                                       aGTGGCGCGAATCTTCGCGCAATCCCGGTGAAGGGCGTTACCCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGgc              <  1:2581322/100‑1 (MQ=255)
                                                                                       aGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGgc              <  2:852500/100‑1 (MQ=255)
                                                                                        attgcgcGAAGATTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTgg               >  1:3714103/4‑98 (MQ=255)
                                                                                        attgcgcGAAGATTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGgcg             >  2:890373/4‑100 (MQ=255)
                                                                                            cgcgAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAAt         >  2:3503303/1‑100 (MQ=255)
                                                                                               gAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAAtttt      <  2:763542/100‑1 (MQ=255)
                                                                                               gAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAAtttt      >  2:1154062/1‑100 (MQ=255)
                                                                                               cgaTCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAAtttt      <  2:1412908/98‑1 (MQ=255)
                                                                                                  tCTTCGCGCAATCCAGGTGAAGGGCGTTAACCAGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTaaa   >  2:3656023/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
AACTGGCAGGAAGCACTGGATCACGTGCAAAAAGTGCCGGGTATTGCCGCTGCCGCGCCGTATATCAATTTCACCGGGCTGGTGGAAAGTGGCGCGAATCTTCGCGCAATCCAGGTGAAGGGCGTTAACCCGCAACAGGAACAGCGTCTGAGCGCATTACCCTCGTTTGTCCAGGGCGATGCGTGGCGCAATTTTAAAG  >  NC_000913/1177548‑1177746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: