BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,338,928	C→A	L107F (TTG→TTT)	mlaE ←	ABC transporter maintaining OM lipid asymmetry, inner membrane permease protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,338,928	0	C	A	100.0%	558.1 / NA	159	L107F (TTG→TTT)	mlaE	ABC transporter maintaining OM lipid asymmetry, inner membrane permease protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (76/83); total (76/83)

Only 100 of 166 total aligned reads displayed.

GCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAG  >  NC_000913/3338831‑3339023

gCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCaaaaaa                                                                                               <  1:1681414/100‑1 (MQ=255)

 cGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaac                                                                                              >  1:844284/1‑100 (MQ=255)

 cGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaac                                                                                              >  1:2722641/1‑100 (MQ=255)

 cGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaac                                                                                              >  2:2399483/1‑100 (MQ=255)

   gATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaacaa                                                                                            >  1:778926/1‑100 (MQ=255)

   gATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaacaa                                                                                            <  1:1770615/100‑1 (MQ=255)

   gATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAaaaaacaa                                                                                            >  2:2775369/1‑100 (MQ=255)

     tCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAAcg                                                                                          <  1:335298/100‑1 (MQ=255)

      ccACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAAcgc                                                                                         <  2:2654803/100‑1 (MQ=255)

       cACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAAcgcg                                                                                        >  1:1053820/1‑100 (MQ=255)

       cACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAAcgcg                                                                                        >  1:1640990/1‑100 (MQ=255)

         ccGCCATAGTCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGc                                                                                      <  1:1779782/100‑1 (MQ=255)

           gCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCaa                                                                                    >  2:56877/1‑100 (MQ=255)

              atcatcTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTGTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAAGAACGCGGCAacca                                                                                 >  1:1345158/1‑100 (MQ=255)

                catcTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAaccacc                                                                               <  2:1485742/100‑1 (MQ=255)

                catcTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAaccacc                                                                               <  1:1723328/100‑1 (MQ=255)

                   ctcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGc                                                                            >  1:1040424/1‑100 (MQ=255)

                    tcCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGcc                                                                           >  1:427161/1‑100 (MQ=255)

                     ccATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGccc                                                                          >  1:1376614/1‑100 (MQ=255)

                      cATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACTGGcccc                                                                         >  1:1403900/1‑100 (MQ=255)

                      cATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGcccc                                                                         >  2:2764718/1‑100 (MQ=255)

                      cATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGcccc                                                                         <  1:1888642/100‑1 (MQ=255)

                      cATACTGGACAGCTGCTCTGTAGCGCGCATCAGGCCGATATCTACGATTAGCACCGAACCAGCACGCCCGGCAACAAACAACGCGGTAACCACCGGcccc                                                                         <  2:2987286/100‑1 (MQ=255)

                        tACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAg                                                                       <  1:2279480/100‑1 (MQ=255)

                          cTGGAGAGTTGGTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGtt                                                                     >  1:1573058/1‑100 (MQ=255)

                          cTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGtt                                                                     >  1:1931523/1‑100 (MQ=255)

                            ggAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCGACCACCGGCCCCAGTTCa                                                                   >  1:2498958/1‑100 (MQ=255)

                            ggAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCa                                                                   >  2:1001377/1‑100 (MQ=255)

                            ggAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCa                                                                   >  1:2700783/1‑100 (MQ=255)

                            ggAGAGCTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTc                                                                    >  1:2248147/1‑99 (MQ=255)

                             gagaGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCAc                                                                  >  2:1783806/1‑100 (MQ=255)

                               gagTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGc                                                                <  1:2752150/100‑1 (MQ=255)

                                 gTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAg                                                              >  1:751599/1‑100 (MQ=255)

                                 gTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAg                                                              >  2:2045577/1‑100 (MQ=255)

                                 gTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAg                                                              >  2:1928456/1‑100 (MQ=255)

                                 gTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAg                                                              >  1:2492915/1‑100 (MQ=255)

                                    gCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAg                                                           >  2:1465126/1‑100 (MQ=255)

                                    gCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAg                                                           >  1:2904135/1‑100 (MQ=255)

                                     ctctGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGc                                                          <  1:1547507/100‑1 (MQ=255)

                                            ncgcgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACg                                                   >  1:2946016/2‑100 (MQ=255)

                                              gcgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGcc                                                 <  1:2030891/100‑1 (MQ=255)

                                               cgcATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGcca                                                >  2:1629772/1‑100 (MQ=255)

                                                 cATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGccacc                                              <  2:844287/100‑1 (MQ=255)

                                                 cATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGccacc                                              <  2:2511627/100‑1 (MQ=255)

                                                  aTCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGccacca                                             >  2:2391890/1‑100 (MQ=255)

                                                   tCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAg                                            <  1:1073856/100‑1 (MQ=255)

                                                   tCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAg                                            <  1:1031370/100‑1 (MQ=255)

                                                    cAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGc                                           >  1:2690808/1‑100 (MQ=255)

                                                     ttccgctcgtCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCa                                          <  1:735950/91‑1 (MQ=255)

                                                     aGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCa                                          <  2:751601/100‑1 (MQ=255)

                                                      ggCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCAt                                         >  1:2815152/1‑100 (MQ=255)

                                                      ggCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCAt                                         >  2:2163512/1‑100 (MQ=255)

                                                      ggCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACCCCACCAGCAt                                         >  1:1963641/1‑100 (MQ=255)

                                                        ccGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATAc                                       >  2:1105042/1‑100 (MQ=255)

                                                         cGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATAcc                                      <  1:2399472/100‑1 (MQ=255)

                                                          gATTTCTGTGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATAccc                                     >  1:219172/1‑100 (MQ=255)

                                                           aTTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCa                                    <  1:2264696/100‑1 (MQ=255)

                                                             ttCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGa                                  <  2:1040429/100‑1 (MQ=255)

                                                              tCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGAc                                 <  1:2573115/100‑1 (MQ=255)

                                                               cTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACt                                <  1:1543257/100‑1 (MQ=255)

                                                               cTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACt                                <  1:493315/100‑1 (MQ=255)

                                                                tGCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTg                               <  2:1752898/100‑1 (MQ=255)

                                                                 gCGGTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTgg                              <  2:2661670/100‑1 (MQ=255)

                                                                   ggTTAGCGCCGAACCAGCACGCCCCGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGtt                            <  2:714203/100‑1 (MQ=255)

                                                                    gTTAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGttt                           <  2:2722656/100‑1 (MQ=255)

                                                                      tAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCCGCATACCCAGACTGGTTTcc                         <  1:1833940/100‑1 (MQ=255)

                                                                      tAGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTcc                         >  1:1458273/1‑100 (MQ=255)

                                                                       aGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCg                        <  2:2591939/100‑1 (MQ=255)

                                                                       aGCGCCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCg                        <  1:56877/100‑1 (MQ=255)

                                                                        gcgcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGc                       <  2:2865269/100‑1 (MQ=255)

                                                                        gcgcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGc                       >  2:138916/1‑100 (MQ=255)

                                                                         cgcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGATTTCCGCa                      >  1:707896/1‑100 (MQ=255)

                                                                          gcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCCGACTGGTTTCCGCa                      >  1:2057064/1‑99 (MQ=255)

                                                                          gcCGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCAc                     <  2:940334/100‑1 (MQ=255)

                                                                            cGAACCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACta                   <  2:2513974/100‑1 (MQ=255)

                                                                              aaCCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACtata                 >  2:744003/1‑100 (MQ=255)

                                                                              aaCCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACtata                 <  2:2999512/100‑1 (MQ=255)

                                                                              aaCCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACtata                 <  2:1376620/100‑1 (MQ=255)

                                                                               aCCAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATaa                <  1:358147/100‑1 (MQ=255)

                                                                                ccAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAg               >  1:1185049/1‑100 (MQ=255)

                                                                                ccAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAg               <  1:771700/100‑1 (MQ=255)

                                                                                 cAGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGt              <  2:819867/100‑1 (MQ=255)

                                                                                  aGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTg             <  1:523762/100‑1 (MQ=255)

                                                                                  aGCACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTg             >  2:1984537/1‑100 (MQ=255)

                                                                                    cACGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGt           <  2:427163/100‑1 (MQ=255)

                                                                                     aCGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTgg            <  1:1463014/98‑1 (MQ=255)

                                                                                     aCGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTc          <  2:1904956/100‑1 (MQ=255)

                                                                                      cGCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCa         <  2:945884/100‑1 (MQ=255)

                                                                                      cGCCCGGCAAAAAACAACGCGGCAACAACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCa         >  2:1826488/1‑100 (MQ=255)

                                                                                       gCCCGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAg        <  2:1426761/100‑1 (MQ=255)

                                                                                        cccGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGa       <  2:2266711/100‑1 (MQ=255)

                                                                                         ccGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGaa      >  2:1315747/1‑100 (MQ=255)

                                                                                          cGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAAc     <  1:1465120/100‑1 (MQ=255)

                                                                                          cGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAAc     <  1:2995577/100‑1 (MQ=255)

                                                                                          cGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAAc     <  2:90415/100‑1 (MQ=255)

                                                                                          cGGCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAAc     <  1:2351647/100‑1 (MQ=255)

                                                                                           ggCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAAcc    <  1:981110/100‑1 (MQ=255)

                                                                                           ggCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAAcc    <  2:1268400/100‑1 (MQ=255)

                                                                                           ggCAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTACCGCACTATAAGTGGTCAGAAcc    <  1:2777167/100‑1 (MQ=255)

                                                                                             cAAAAAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAg  >  2:2408780/1‑100 (MQ=255)

GCGGATCCACCGCCATCATCTCCATACTGGAGAGTTGCTCTGTAGCGCGCATCAGGCCGATTTCTGCGGTTAGCGCCGAACCAGCACGCCCGGCAAACAACAACGCGGCAACCACCGGCCCCAGTTCACGCAGTAGCGATAACGCCACCAGCATACCCAGACTGGTTTCCGCACTATAAGTGGTCAGAACCAG  >  NC_000913/3338831‑3339023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`