Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,186,293	C→T	A315V (GCG→GTG)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,186,293	0	C	T	100.0%	339.3 / NA	95	A315V (GCG→GTG)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (47/48);  total (47/48)

GGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGT  >  NC_000913/4186198‑4186389                                                                                                |                                                                                                 ggATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATc                                                                                              <  1:1964105/100‑1 (MQ=255) ggATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATc                                                                                              <  2:268647/100‑1 (MQ=255)   aTCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGTGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCa                                                                                             >  1:2761638/1‑99 (MQ=255)   aTCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCAc                                                                                            >  2:179835/1‑100 (MQ=255)    tCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCAcc                                                                                           >  1:1062579/1‑100 (MQ=255)       ngCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGt                                                                                        >  1:354258/2‑100 (MQ=255)       ggCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGGCGTGTGATCACCgg                                                                                         >  1:1934341/1‑99 (MQ=255)        gCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGtt                                                                                       <  1:2606021/100‑1 (MQ=255)         cTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTc                                                                                      <  1:1402557/100‑1 (MQ=255)         cTGCGCCGGACATCATCGTACGTAACCAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTc                                                                                      <  1:595880/100‑1 (MQ=255)           gcgcCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTa                                                                                    <  2:1412688/100‑1 (MQ=255)           gcgcCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTa                                                                                    <  1:2134311/100‑1 (MQ=255)            cgcCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTaa                                                                                   <  2:95344/100‑1 (MQ=255)             gcCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAAc                                                                                  <  1:1118620/100‑1 (MQ=255)             gcCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAAc                                                                                  >  2:808569/1‑100 (MQ=255)             gcCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGATCTaa                                                                                   >  1:111859/1‑99 (MQ=255)              ccGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACa                                                                                 <  1:2565261/100‑1 (MQ=255)              ccGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACa                                                                                 >  2:1498219/1‑100 (MQ=255)                ggACATCATCGTACGTAACGAAAATCGTATGCTGCAGGAAGCGGTAGTCGCCCTGCTGGATAACGGTCGTCGCGGTCCTGTGATCACCGGTTCTAACAAg                                                                               <  1:2796419/100‑1 (MQ=255)                ggACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAg                                                                               >  1:2111493/1‑100 (MQ=255)                       atcGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCctc                                                                        >  1:1631095/1‑100 (MQ=255)                        tcGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCctct                                                                       <  2:2788407/100‑1 (MQ=255)                         cgtacgtaACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTg                                                                      <  2:1016080/100‑1 (MQ=255)                          ntacgtaACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGa                                                                     >  1:1555516/2‑100 (MQ=255)                           tacgtaACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGaa                                                                    >  2:2001608/1‑100 (MQ=255)                             cgtaACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAAt                                                                  <  2:1687029/100‑1 (MQ=255)                             cgtaACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAAt                                                                  <  2:1687131/100‑1 (MQ=255)                               taACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCt                                                                >  2:230845/1‑100 (MQ=255)                                aaCGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCtt                                                               >  1:624592/1‑100 (MQ=255)                                aaCGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCtt                                                               <  1:155114/100‑1 (MQ=255)                                 aCGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCttt                                                              >  1:2285705/1‑100 (MQ=255)                                  cGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTg                                                             <  1:1090237/100‑1 (MQ=255)                                  cGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTg                                                             >  2:1792245/1‑100 (MQ=255)                                   gAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTgg                                                            >  2:936844/1‑100 (MQ=255)                                   gAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTgg                                                            >  1:438315/1‑100 (MQ=255)                                   gAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTgg                                                            <  1:1440730/100‑1 (MQ=255)                                   gAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTg                                                             <  1:883987/99‑1 (MQ=255)                                    aaaaaCGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGc                                                           <  1:167528/100‑1 (MQ=255)                                     aaaaCGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGcc                                                          >  1:462211/1‑100 (MQ=255)                                     aaaaCGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGcc                                                          >  1:2622610/1‑100 (MQ=255)                                     aaaaCGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGcc                                                          >  2:2685359/1‑100 (MQ=255)                                      aaaCGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCg                                                         <  2:2676739/100‑1 (MQ=255)                                       aaCGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCg                                                         >  1:1062201/1‑99 (MQ=255)                                              gctgcAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATc                                                 >  1:2857982/1‑100 (MQ=255)                                              gctgcAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATc                                                 >  2:664597/1‑100 (MQ=255)                                               ctgcAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCa                                                <  1:2342508/100‑1 (MQ=255)                                                 gcAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCaaa                                              >  2:1559629/1‑100 (MQ=255)                                                  cAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAg                                             <  1:100800/100‑1 (MQ=255)                                                  cAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAg                                             >  1:1169846/1‑100 (MQ=255)                                                  cAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAg                                             <  2:319771/100‑1 (MQ=255)                                                  cAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAg                                             <  1:2443240/100‑1 (MQ=255)                                                   aGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTGACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAgg                                            <  2:1520978/100‑1 (MQ=255)                                                   aGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAgg                                            <  1:2329909/100‑1 (MQ=255)                                                   aGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAgg                                            <  2:2437331/100‑1 (MQ=255)                                                   aGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAgg                                            <  2:1521063/100‑1 (MQ=255)                                                      aagtggTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTaa                                         <  2:2761653/96‑1 (MQ=255)                                                          ggTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAg                                     >  1:2895694/1‑100 (MQ=255)                                                          ggTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAg                                     <  2:316264/100‑1 (MQ=255)                                                            tAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAggg                                   >  2:1094354/1‑100 (MQ=255)                                                             aGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGt                                  <  2:329314/100‑1 (MQ=255)                                                             aGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGt                                  >  2:1544491/1‑100 (MQ=255)                                                               aCGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCg                                >  2:2690883/1‑100 (MQ=255)                                                                cGCCCTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGt                               >  1:1924427/1‑100 (MQ=255)                                                                  cccTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGttt                             >  1:2634571/1‑100 (MQ=255)                                                                  cccTGCTGGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGttt                             >  1:2634495/1‑100 (MQ=255)                                                                    ctgctgGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTcc                           >  1:2824859/1‑100 (MQ=255)                                                                     tgctgGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCg                          >  2:1949585/1‑100 (MQ=255)                                                                      gctgGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGt                         <  2:149462/100‑1 (MQ=255)                                                                      gctgGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGt                         <  1:2001600/100‑1 (MQ=255)                                                                       ctgGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTc                        <  1:21510/100‑1 (MQ=255)                                                                        tgGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCa                       <  1:179834/100‑1 (MQ=255)                                                                        tgGATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCa                       >  2:2035852/1‑100 (MQ=255)                                                                         ggATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAg                      >  1:570975/1‑100 (MQ=255)                                                                         ggATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAg                      >  2:1653043/1‑100 (MQ=255)                                                                         ggATAACGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAg                      >  1:2949503/1‑100 (MQ=255)                                                                          gATAACGGTTGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGATATGATCAAAGGTAAACAGGGTCGTTTCCGTCAg                      <  1:1963280/99‑1 (MQ=255)                                                                             aaCGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAAcc                  <  1:1498211/100‑1 (MQ=255)                                                                              aCGGTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCt                 >  1:374769/1‑100 (MQ=255)                                                                              aCGGTCGTCGCGGTCCTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCt                 <  2:2949520/100‑1 (MQ=255)                                                                                ggTCGTCGCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGc               <  2:589639/100‑1 (MQ=255)                                                                                  tcgtcgCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTc             <  1:1277491/100‑1 (MQ=255)                                                                                   cgtcgCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCg            >  2:777498/1‑100 (MQ=255)                                                                                    gtcgCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCgg           >  2:2437403/1‑100 (MQ=255)                                                                                    gtcgCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCgg           <  2:474615/100‑1 (MQ=255)                                                                                    gtcgCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCgg           >  1:887978/1‑100 (MQ=255)                                                                                     tcgCGGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGt          <  2:1078637/100‑1 (MQ=255)                                                                                      cgcgGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTa         >  1:203173/1‑100 (MQ=255)                                                                                      cgcgGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTa         >  2:909146/1‑100 (MQ=255)                                                                                      cgcgGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTa         >  1:62687/1‑100 (MQ=255)                                                                                        cgGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAg       <  2:2994340/100‑1 (MQ=255)                                                                                        cgGTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAg       <  2:2737269/100‑1 (MQ=255)                                                                                         ggTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGc      >  1:1094042/1‑100 (MQ=255)                                                                                         ggTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGc      <  2:1436676/100‑1 (MQ=255)                                                                                         ggTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGc      <  2:688175/100‑1 (MQ=255)                                                                                          gTCGTGTGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGcn     <  1:2449792/100‑2 (MQ=255)                                                                                             gtgtGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCgtgt  >  2:440376/1‑100 (MQ=255)                                                                                                |                                                                                                 GGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGT  >  NC_000913/4186198‑4186389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑