New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4614759 =	181 (1.410)	8 (0.070)	8/170	NT	4.7%	coding (785/864 nt)	yjjW	putative pyruvate formate lyase activating enzyme
	?	NC_000913	4614791 =	150 (1.230)					coding (753/864 nt)	yjjW	putative pyruvate formate lyase activating enzyme
Rejected: Frequency below cutoff threshold.

TGGCGGCGTTTATCAAGGGACTCGGCGATGTTCCGGTACGCCTGAACGCGTTTCATGCCCACGGCGTGTATGGCGAGGCGCAAAGCTGGGCGAGCGCCACGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4614861‑4614759 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgccATACACGCCGTGGGCATGAAACGCGTTCAGGCGTACCGGAACATCGCCGAGTCCCTTGATAAACGCCGCCAGTTCTTCGATGTGTTGCAAATAATCCA  >  NC_000913/4614791‑4614888                                                                                                                                                                                                           TGGCGGCGTTTATCAAGGGACTCGGCGATGTTCCGGTACGCCTGAACGCGTTTCATGCCCACGGCGTGTATGGCGAGGCGCAAAGCTGGGCGAGCGCCAC                                                                                                       <  2:596385/100‑1  GGCGGCGTTTATCAAGGGACTCGGCGATGTTCCGGTACGCCTGAACGCGTTTCATGCCCACGGCGTGTATGGCGAGGCGCAAAGCTGGGCGAGCGCCACG                                                                                                      >  1:780107/1‑100    CGGCGTTTATCAAGGGACTCGGCGATGTTCCGGTACGCCTGAACGCGTTTCATGCCCACGGCGTGTATGGCGAGGCGCAAAGCTGGGCGAGCGCCACGCC                                                                                                    >  1:1362025/1‑100              CAAGGGACTCGGCGATGTTCCGGTACGCCTGAACGCGTTTCATGCCCACGGCGTGTATGGCGAGGCGCAAAGCTGGGCGAGCGCCACGCCATACACGCCG                                                                                          >  1:2266135/1‑100                      TCGGCGATGTTCCGGTACGCCTGAACGCGTTTCATGCCCACGGCGTGTATGGCGAGGCGCAAAGCTGGGCGAGCGCCACGCCATACACGCCGTGGGCATG                                                                                  <  2:2266149/100‑1                                        GCCTGAACGCGTTTCATGCCCACGGCGTGTATGGCGAGGCGCAAAGCTGGGCGAGCGCCACGCCATAAAAGCCGTGGGCATGAAACGCGTTCAGGCGTAC                                                                >  1:685876/1‑100                                                          CCCACGGCGTGTATGGCGAGGCGCAAAGCTGGGCGAGCGCCACGCCATACACGCCGTGGGCATGAAACGCGTTCAGGCGTACCGGAACATCGCCGAGTCC                                              >  2:3204193/1‑100                                                                    GTATGGCGAGGCGCAAAGCTGGGCGAGCGCCACGCCGTACACGCCGTGGGCATGAAACGCGTTCAGGCGTACCGGAACATCGCCGAGTCCCTTGATAAAC                                    >  1:3022710/1‑100                                                                    GTATGGCGAGGCGCAAAGCTGGGCGAGCGCCACGCCATACACGCCGTGGGCATGAAACGCGTTCAGGCGTACCGGAACATCGCCGAGTCCCTTGATAAAC                                    <  1:3204171/100‑1                                                                       TGGCGAGGCGCAAAGCTGGGCGAGCGCCACGCCATACACGCCGTGGGCATGAAACGCGTTCAGGCGTACCGGAACATCGCCGAGTCCCTTGATAAACGCC                                 >  2:2382885/1‑100                                                                         GCGAGGCGCAAAGCTGGGCGAGCGCCACGCCATACACGCCGTGGGCATGAAACGCGTTCAGGCGTACCGGAACATCGCCGAGTCCCTTGATAAACGCCGC                               >  1:3386396/1‑100                                                                                                    CGCCATACACGCCGTGGGCATGAAACGCGTTCAGGCGTACCGGAACATCGCCGAGTCCCTTGATAAACGCCGCCAGTTCTTCGATGTGTTGCAAATAATC    >  2:1824227/1‑100                                                                                                    CGCCATACACGCCGTGGGCATGAAACGCGTTCAGGCGTACCGGAACATCGCCGAGTCCCTTGATAAACGCCGCCAGTTCTTCGATGTGTTGCAAATAATC    >  2:202692/1‑100                                                                                                     GCCATACACGCCGTGGGCATGAAACGCGTTCAGGCGTACCGGAACATCGCCGAGTCCCTTGATAAACGCCGCCAGTTCTTCGATGTGTTGCAAATAATCC   <  2:1930651/100‑1                                                                                                      CCATACACGCCGTGGGCATGAAACGCGTTCAGGCGTACCGGAACATCGCCGAGTCCCTTGATAAACGCCGCCAGTTCTTCGATGTGTTGCAAATAATCCA  >  2:524726/1‑100                                                                                                      CCATACACGCCGTGGGCATGAAACGCGTTCAGGCGTACCGGAACATCGCCGAGTCCCTTGATAAACGCCGCCAGTTCTTCGATGTGTTGCAAATAATCCA  <  1:1660162/100‑1                                                                                                      CCATACACGCCGTGGGCATGAAACGCGTTCAGGCGTACCGGAACATCGCCGAGTCCCTTGATAAACGCCGCCAATTCTTCGATGTGTTGCAAATAATCCA  <  2:2169325/100‑1                                                                                                                                                                                                           TGGCGGCGTTTATCAAGGGACTCGGCGATGTTCCGGTACGCCTGAACGCGTTTCATGCCCACGGCGTGTATGGCGAGGCGCAAAGCTGGGCGAGCGCCACGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4614861‑4614759 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgccATACACGCCGTGGGCATGAAACGCGTTCAGGCGTACCGGAACATCGCCGAGTCCCTTGATAAACGCCGCCAGTTCTTCGATGTGTTGCAAATAATCCA  >  NC_000913/4614791‑4614888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.