Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC NC_000913 257,908 Δ776 bp 100% [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908–258676 258683 8–776 163 [0] [0] 162 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)134 (1.150) 75/162 NT 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

Only 100 of 162 total aligned reads displayed.

TGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGC  >  NC_000913/258585‑258782
                                                                                                   |                                                                                                  
catcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaa                                                                                                    >  2:2084252‑M2/100‑100 (MQ=255)
 atcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAg                                                                                                   >  2:92042‑M2/99‑100 (MQ=255)
 atcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAg                                                                                                   >  2:1212468‑M2/99‑100 (MQ=255)
 atcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAg                                                                                                   <  1:2580005‑M2/2‑1 (MQ=255)
   cacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTg                                                                                                 <  1:3404257‑M2/4‑1 (MQ=255)
     caacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCa                                                                                               >  1:2436528‑M2/95‑100 (MQ=255)
        caggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAg                                                                                            >  1:2630955‑M2/92‑100 (MQ=255)
         aggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaatgtaAGTGCAAAGa                                                                                           <  2:2388325‑M2/10‑1 (MQ=255)
          ggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGAt                                                                                          >  2:1717949‑M2/90‑100 (MQ=255)
          ggagatagcaatgacgttaccgagtggacacccgaagagcagactgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGAt                                                                                          <  1:2277338‑M2/11‑1 (MQ=255)
           gagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATa                                                                                         <  1:2750868‑M2/12‑1 (MQ=255)
              atagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATc                                                                                      <  2:1611902‑M2/15‑1 (MQ=255)
                  caatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAtt                                                                                  >  2:2709418‑M2/82‑100 (MQ=255)
                   aatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTc                                                                                 >  1:2020025‑M2/81‑100 (MQ=255)
                        cgttacctagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                            >  2:1865864‑M2/76‑100 (MQ=255)
                        cgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCtttt                                                                             >  1:1087044‑M2/76‑99 (MQ=255)
                         gttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTc                                                                           <  1:578436‑M2/26‑1 (MQ=255)
                         gttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTc                                                                           <  2:876223‑M2/26‑1 (MQ=255)
                            accgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAt                                                                        <  1:3048546‑M2/29‑1 (MQ=255)
                             ccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAtt                                                                       >  2:3442804‑M2/71‑100 (MQ=255)
                             ccgagtggacacccgaagagcaaaataaaaaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAtt                                                                       <  1:871846‑M2/30‑1 (MQ=255)
                               gagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGt                                                                     >  2:2187841‑M2/69‑100 (MQ=255)
                                   ggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                 >  2:2633979‑M2/65‑100 (MQ=255)
                                    gacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGc                                                                <  2:2721083‑M2/37‑1 (MQ=255)
                                    gacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGc                                                                <  1:2957469‑M2/37‑1 (MQ=255)
                                     acacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCt                                                               <  1:1143089‑M2/38‑1 (MQ=255)
                                      cacccgaagagcagattgatcacaaaatttagcgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTg                                                              <  1:2396196‑M2/39‑1 (MQ=255)
                                      cacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTg                                                              >  1:1132644‑M2/62‑100 (MQ=255)
                                        cccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                            >  2:2785316‑M2/60‑100 (MQ=255)
                                        cccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                            >  2:3164741‑M2/60‑100 (MQ=255)
                                        cccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                            >  2:3062012‑M2/60‑100 (MQ=255)
                                        cccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                            >  2:2035973‑M2/60‑100 (MQ=255)
                                        cccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                            >  1:96326‑M2/60‑100 (MQ=255)
                                        cccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                            >  2:2471961‑M2/60‑100 (MQ=255)
                                        cccgaagagcagattgatcaaaaaatttaccgaactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                            <  1:660876‑M2/41‑1 (MQ=255)
                                         ncgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTAt                                                           >  2:530277‑M2/59‑100 (MQ=255)
                                         ccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTAt                                                           >  2:2463293‑M2/59‑100 (MQ=255)
                                          cgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATg                                                          >  1:1153043‑M2/58‑100 (MQ=255)
                                              gagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTc                                                      <  2:1411003‑M2/47‑1 (MQ=255)
                                                gcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCaa                                                    >  2:1733526‑M2/52‑100 (MQ=255)
                                                gcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCaa                                                    >  1:1513490‑M2/52‑100 (MQ=255)
                                                  agattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACg                                                  <  2:2246524‑M2/51‑1 (MQ=255)
                                                  agattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACg                                                  >  2:706252‑M2/50‑100 (MQ=255)
                                                   gattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAAc                                                   >  1:1512752‑M2/49‑98 (MQ=255)
                                                    attgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTg                                                <  2:1877510‑M2/53‑1 (MQ=255)
                                                    attgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTg                                                >  2:1233332‑M2/48‑100 (MQ=255)
                                                     ttgatcaataaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGa                                               >  2:1448943‑M2/47‑100 (MQ=255)
                                                      tgatcaaaaactttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGaa                                              <  1:2932410‑M2/55‑1 (MQ=255)
                                                      tgatcaaaaaatttaccgcactagtcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGaa                                              <  1:3277691‑M2/55‑1 (MQ=255)
                                                       gatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGaaa                                             >  1:2215412‑M2/45‑100 (MQ=255)
                                                          caaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACgtgaaagat                                          >  1:2029044‑M2/42‑97 (MQ=255)
                                                          caaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCg                                          >  1:250689‑M2/42‑100 (MQ=255)
                                                          caaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCg                                          >  1:704024‑M2/42‑100 (MQ=255)
                                                          caaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCg                                          >  2:2083196‑M2/42‑100 (MQ=255)
                                                          caaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCg                                          >  2:200401‑M2/42‑100 (MQ=255)
                                                          caaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCg                                          >  1:2691175‑M2/42‑100 (MQ=255)
                                                          caaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCg                                          >  2:1770644‑M2/42‑100 (MQ=255)
                                                           aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                         >  1:2306154‑M2/41‑100 (MQ=255)
                                                           aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                         >  1:1349045‑M2/41‑100 (MQ=255)
                                                           aaaaaatttaccgcactaggcccgtatattcgtgaaggaaAGTGCAAAAATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGaaa                                             >  1:1239189‑M2/41‑96 (MQ=255)
                                                            aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTTTTTTTTTTTTCTGTATGCGTCAACGTGAAAcc                                           >  1:825111‑M2/40‑97 (MQ=255)
                                                            aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGc                                        <  2:2426387‑M2/61‑1 (MQ=255)
                                                            aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGc                                        <  1:2678445‑M2/61‑1 (MQ=255)
                                                            aaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGc                                        >  1:1054923‑M2/40‑100 (MQ=255)
                                                              aaatttaccgtactaggcacgtatattcttgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCAc                                      <  1:1273529‑M2/63‑1 (MQ=255)
                                                              aaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCAc                                      <  1:1163545‑M2/63‑1 (MQ=255)
                                                                 tttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCgg                                   <  2:1890276‑M2/66‑1 (MQ=255)
                                                                    accgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAg                                <  1:1068751‑M2/69‑1 (MQ=255)
                                                                    accgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAg                                <  1:901859‑M2/69‑1 (MQ=255)
                                                                    accgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAg                                <  1:2905311‑M2/69‑1 (MQ=255)
                                                                    accgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAg                                <  2:794531‑M2/69‑1 (MQ=255)
                                                                     ccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGt                               >  1:215921‑M2/31‑100 (MQ=255)
                                                                      cgcactaggcccgtatattcgtgaatgtaAGTGCAAATATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTg                              <  1:2721956‑M2/71‑1 (MQ=255)
                                                                      cgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGt                               >  1:739320‑M2/30‑99 (MQ=255)
                                                                       gcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGc                             <  1:3450171‑M2/72‑1 (MQ=255)
                                                                        cactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTTTATTTTTTTGCTGTATGCGTCAACGTGAAATCGGCACCGGAAGTGCg                            >  1:1016934‑M2/28‑100 (MQ=255)
                                                                        cactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCg                            >  2:235679‑M2/28‑100 (MQ=255)
                                                                        cactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCg                            <  2:308290‑M2/73‑1 (MQ=255)
                                                                         actaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTTGTTTTTTTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                           >  1:1317318‑M2/27‑100 (MQ=255)
                                                                          ctagtcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTg                          <  1:1826723‑M2/75‑1 (MQ=255)
                                                                          ctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTg                          >  1:1004771‑M2/26‑100 (MQ=255)
                                                                              gcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAAtt                      <  1:3164719‑M2/79‑1 (MQ=255)
                                                                                 cgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTg                   >  2:3345696‑M2/19‑100 (MQ=255)
                                                                                  gtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgg                  <  1:1590250‑M2/83‑1 (MQ=255)
                                                                                     tattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGc               >  2:1096739‑M2/15‑100 (MQ=255)
                                                                                        tcgtgaaggtaAGTGCAAAGATAATCGATTCTATTTCGACTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtgg            <  1:2499150‑M2/89‑1 (MQ=255)
                                                                                         cgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATACGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtgg            >  2:713945‑M2/11‑99 (MQ=255)
                                                                                          gtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtg          >  1:2382721‑M2/10‑100 (MQ=255)
                                                                                          gtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtg          <  1:2140490‑M2/91‑1 (MQ=255)
                                                                                          gtgaaagtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAGCCAGCACCGGAAGTGCGTGAATTCTGGGGCtgg            <  1:3096236‑M2/89‑1 (MQ=255)
                                                                                           tgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtgg         >  2:419237‑M2/9‑100 (MQ=255)
                                                                                              aggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatg      <  1:1614870‑M2/95‑1 (MQ=255)
                                                                                              aggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatg      <  2:1669460‑M2/95‑1 (MQ=255)
                                                                                              aggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatg      <  2:3364292‑M2/95‑1 (MQ=255)
                                                                                               ggtaAGTGCAAAGATAATCGATTCTTTTTCTTTTTTTTTTCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggat       >  1:976026‑M2/5‑98 (MQ=255)
                                                                                               ggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatgg     <  2:502295‑M2/96‑1 (MQ=255)
                                                                                               ggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatgg     >  1:2838583‑M2/5‑100 (MQ=255)
                                                                                                 taAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAg   >  1:855725‑M2/3‑100 (MQ=255)
                                                                                                  aAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGc  <  2:3461135‑M2/99‑1 (MQ=255)
                                                                                                  aAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGc  <  2:96327‑M2/99‑1 (MQ=255)
                                                                                                   |                                                                                                  
TGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGC  >  NC_000913/258585‑258782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: