Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 83,590 G→T 15.1% intergenic (‑61/+32) leuA ← / ← leuL 2‑isopropylmalate synthase/leu operon leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091383,5900GT15.1% 482.3 / 68.4 175intergenic (‑61/+32)leuA/leuL2‑isopropylmalate synthase/leu operon leader peptide
Reads supporting (aligned to +/- strand):  ref base G (61/87);  new base T (12/15);  total (73/102)
Fisher's exact test for biased strand distribution p-value = 8.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.17e-01

Only 100 of 180 total aligned reads displayed.

GCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAA  >  NC_000913/83491‑83689
                                                                                                   |                                                                                                   
gcgcAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATg                                                                                                     >  2:2189363/1‑100 (MQ=255)
 cgcAATGTGGTATCGAAAATAATGACTTGTTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATg                                                                                                     <  1:2091100/99‑1 (MQ=255)
     aTGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCGTTTAGAGCGCTTCTATTCGGGCATAAAAAAACCCGCGCAATGGcgcg                                                                                                <  1:628447/100‑1 (MQ=255)
      tgtgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCgg                                                                                               <  2:1816994/100‑1 (MQ=255)
      tgtgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCgg                                                                                               <  2:3352189/100‑1 (MQ=255)
      tgtgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCgg                                                                                               <  2:1586729/100‑1 (MQ=255)
       gtgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCggg                                                                                              >  2:2497583/1‑100 (MQ=255)
        tgGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGt                                                                                             <  2:2119393/100‑1 (MQ=255)
         ggTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGACATAAAAAAACCCGCGCAATGGCGCGGGtt                                                                                            <  1:3236871/100‑1 (MQ=255)
          gTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGttt                                                                                           >  1:1494820/1‑100 (MQ=255)
           tATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGcttt                                                                                          >  1:1657443/1‑100 (MQ=255)
            aTCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGttttt                                                                                         <  1:904284/100‑1 (MQ=255)
            aTCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTGAGAGCGCCTCGCTTCGTGCATAAAAAAACCCGCGCCATTGCGCGGGttttt                                                                                         <  1:227839/100‑1 (MQ=38)
             tCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGtttttt                                                                                        <  2:1459704/100‑1 (MQ=255)
               gAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgt                                                                                      <  2:1501402/100‑1 (MQ=255)
                aaaaTAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgtt                                                                                     <  1:2317064/100‑1 (MQ=255)
                aaaaTAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgtt                                                                                     <  2:2808172/100‑1 (MQ=255)
                aaaaTAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgtt                                                                                     <  2:825732/100‑1 (MQ=255)
                aaaaTAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTCTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgtt                                                                                     <  1:2495626/100‑1 (MQ=255)
                  aataatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgtttg                                                                                   <  1:3279881/100‑1 (MQ=255)
                   ataatGACTTGCTGGCTCATGGTTCGGGTCCTTGTCTCTTTTGGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgttt                                                                                    <  1:2016221/98‑1 (MQ=255)
                    taatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGAc                                                                                 >  2:925891/1‑100 (MQ=255)
                      atGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTg                                                                               >  1:861602/1‑100 (MQ=255)
                      atGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTg                                                                               >  2:424760/1‑100 (MQ=255)
                      atGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTg                                                                               <  2:2026747/100‑1 (MQ=255)
                      atGACTTGCTGGCACATGGTATGAGACCTTGTCTCTTTTAAAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGAAATGGCGCGAGTTTTTTGTATGACTg                                                                               >  1:252605/1‑100 (MQ=255)
                          cTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTg                                                                           >  2:3479791/1‑100 (MQ=255)
                           ttGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGc                                                                          <  2:1145988/100‑1 (MQ=255)
                           ttGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGCTTTTTGTTTGACTGCGt                                                                            >  1:2112574/1‑98 (MQ=255)
                            tGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCt                                                                         <  2:2485259/100‑1 (MQ=255)
                             gCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTg                                                                        <  2:338670/100‑1 (MQ=255)
                              cTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTgg                                                                       <  2:21469/100‑1 (MQ=255)
                               tGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGc                                                                      <  2:1045860/100‑1 (MQ=255)
                                 gCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCtt                                                                    >  1:920696/1‑100 (MQ=255)
                                  cacATGGTTTGGGACCTTGTCTCTTTTAGAGAGCCTCGCTTCAGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTa                                                                   >  2:2399266/3‑100 (MQ=255)
                                   tCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTaa                                                                  >  1:328383/1‑100 (MQ=255)
                                   tCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTaa                                                                  >  2:1443596/1‑100 (MQ=255)
                                    cATGGTTTGGTTCCTTGTCTCTTTTAGAGCGTCTCGCTTCGCGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTTACTGCGTGCTGGCTTAAt                                                                 <  2:3264984/100‑1 (MQ=255)
                                    cATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAAt                                                                 >  1:1834320/1‑100 (MQ=255)
                                     aTGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATg                                                                <  1:3381295/100‑1 (MQ=255)
                                       ggTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCt                                                              >  1:3071847/1‑100 (MQ=255)
                                        gTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTg                                                             >  1:1169023/1‑100 (MQ=255)
                                        gTTTGGGTCCTTGTCTCTTTGAGAGCGCCTCGCTTCGGGCATAAAAAATCCCGCGCTATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTCAAt                                                                 <  1:3190480/96‑1 (MQ=255)
                                         tttGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTGCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTgg                                                            <  2:1763482/100‑1 (MQ=255)
                                           tGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGAt                                                          <  1:1049791/100‑1 (MQ=255)
                                            gggTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATg                                                         >  2:1434121/1‑100 (MQ=255)
                                            gggTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATg                                                         >  1:1270589/1‑100 (MQ=255)
                                             ggTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGc                                                        >  2:1389563/1‑100 (MQ=255)
                                              gTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGcc                                                       <  1:1287160/100‑1 (MQ=255)
                                               tCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAACACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCg                                                      >  2:2104618/1‑100 (MQ=255)
                                                ccTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAATCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGc                                                     <  2:489024/100‑1 (MQ=255)
                                                 catGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCt                                                    >  2:2401216/3‑100 (MQ=255)
                                                 cTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGccaga                                                    >  1:2125894/1‑97 (MQ=255)
                                                    gTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCAc                                                 <  2:790904/100‑1 (MQ=255)
                                                       tctTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCg                                              <  2:2543312/100‑1 (MQ=255)
                                                        ctTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGt                                             <  2:1210997/100‑1 (MQ=255)
                                                        ctTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGt                                             >  2:900125/1‑100 (MQ=255)
                                                         ttttAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTc                                            >  2:1678692/1‑100 (MQ=255)
                                                            tAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTAc                                         >  2:2229232/1‑100 (MQ=255)
                                                             agagCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTAcc                                        <  1:131785/100‑1 (MQ=255)
                                                               agCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACcgc                                      >  2:2593097/1‑100 (MQ=255)
                                                                gcgcCTCGCTGCGCGCAAAAGAAAGACCGCGCGTTGGCGCGTGTGTGGTGTGTGGCTGTGGGCTGGCTGAATGGTGGATGCCGCTCACTCGTCTACcgc                                      <  1:2425314/99‑1 (MQ=255)
                                                                  gcCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCgcgc                                    >  1:980138/1‑99 (MQ=255)
                                                                  gcCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCa                                   >  2:2346900/1‑100 (MQ=255)
                                                                  gcCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCa                                   <  1:200445/100‑1 (MQ=255)
                                                                  gcCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCa                                   <  1:1389556/100‑1 (MQ=255)
                                                                   ccTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCaa                                  >  2:665961/1‑100 (MQ=255)
                                                                    ctcttctCGGGCATCACACCACCCGCGCAGTGGCGCGGGTTTTTTGTTTGCCTGCGTGCAGGCTTAATGCTGGATGCCGCCCACTCGTCTACCGCGCaaa                                 <  1:3014890/94‑1 (MQ=255)
                                                                      cGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAaaga                               <  1:730927/100‑1 (MQ=255)
                                                                       gCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAaagaa                              <  2:3206847/100‑1 (MQ=255)
                                                                           cGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATg                          >  2:1990213/1‑100 (MQ=255)
                                                                           cGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATg                          >  2:1123052/1‑100 (MQ=255)
                                                                            gggCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGc                         <  1:3183390/100‑1 (MQ=255)
                                                                            ggacATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGc                         >  1:1722804/4‑100 (MQ=255)
                                                                              caaacaaaaaaCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGt                       >  2:2700496/6‑100 (MQ=38)
                                                                               cATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGtt                      <  2:289851/100‑1 (MQ=255)
                                                                                aacaaaaaaCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGttt                     >  2:2000466/4‑100 (MQ=38)
                                                                                aTAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGttt                     <  1:1697308/100‑1 (MQ=255)
                                                                                 tAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTta                    >  2:752297/1‑100 (MQ=255)
                                                                                 tAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTta                    <  2:2702062/100‑1 (MQ=255)
                                                                                 acaaaaaaCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTta                    >  2:3086092/3‑100 (MQ=255)
                                                                                 acaaaaaaCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTta                    >  1:510163/3‑100 (MQ=255)
                                                                                   aaaaaaCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagt                  >  2:2755414/1‑100 (MQ=255)
                                                                                      ataCCCGCGCAATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGTTGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagt                  <  1:3498681/95‑1 (MQ=255)
                                                                                       aaCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCACTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagta              >  1:437400/1‑100 (MQ=39)
                                                                                        aCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtag             >  2:564144/1‑100 (MQ=255)
                                                                                        aCCCGCGCAATGGTGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGTTGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtag             <  1:1966834/100‑1 (MQ=255)
                                                                                        aCCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtag             <  2:1084761/100‑1 (MQ=255)
                                                                                         cccGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagt            >  2:3517942/1‑100 (MQ=255)
                                                                                         cccGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagt            >  2:71661/1‑100 (MQ=255)
                                                                                         cccGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagt            <  2:3113806/100‑1 (MQ=255)
                                                                                         cccGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagt            >  2:2211234/1‑100 (MQ=255)
                                                                                         cccGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagt            >  2:34705/1‑100 (MQ=255)
                                                                                         cccGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagt            <  2:1169030/100‑1 (MQ=255)
                                                                                           cgcgCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagtag          >  1:1773836/1‑100 (MQ=255)
                                                                                           cgcgCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagtagtagtag          >  2:1942631/1‑100 (MQ=255)
                                                                                              gcAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGAcc       <  1:546660/100‑1 (MQ=255)
                                                                                                  tGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATa   <  1:1096594/100‑1 (MQ=255)
                                                                                                  tGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATa   <  1:1011412/100‑1 (MQ=255)
                                                                                                   ggCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATaa  >  2:757608/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
GCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAA  >  NC_000913/83491‑83689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: