BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	263,172	A→C	100%	pseudogene (41/63 nt)	ykfN ←	pseudogene, YdiA family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	263,172	0	A	C	99.2%	483.0 / ‑4.0	140	pseudogene (41/63 nt)	ykfN	pseudogene, YdiA family
Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (68/69); total (69/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 140 total aligned reads displayed.

GGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTAGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACG  >  NC_000913/263075‑263268

ggCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGc                                                                                                <  1:2608679/100‑1 (MQ=255)

 gCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCt                                                                                               >  1:2238518/1‑100 (MQ=255)

 gCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCt                                                                                               >  2:285505/1‑100 (MQ=255)

 gCTTCGCTCACTATACATCCATGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTAAAGGTGTTGGCAATGTGTTCAATAAAGCCCGAACAAATTCGc                                                                                                >  1:648430/1‑99 (MQ=255)

  cTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTc                                                                                              <  2:620462/100‑1 (MQ=255)

    tCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCAt                                                                                            <  1:2546599/100‑1 (MQ=255)

      gCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTa                                                                                          >  2:714513/1‑100 (MQ=255)

       cTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTAt                                                                                         >  2:282651/1‑100 (MQ=255)

         cACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGa                                                                                       >  1:106083/1‑100 (MQ=255)

          aCTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGAt                                                                                      >  2:3013622/1‑100 (MQ=255)

          aCTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGAt                                                                                      <  2:1572448/100‑1 (MQ=255)

           cTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATc                                                                                     >  2:3305669/1‑100 (MQ=255)

           cTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATc                                                                                     >  2:561528/1‑100 (MQ=255)

            tataCATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCg                                                                                    >  2:2917398/1‑100 (MQ=255)

            tataCATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCg                                                                                    >  2:2688010/1‑100 (MQ=255)

            tataCATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCg                                                                                    <  1:1900384/100‑1 (MQ=255)

             ataCATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCgg                                                                                   <  1:728476/100‑1 (MQ=255)

             ataCATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCgg                                                                                   >  1:1035794/1‑100 (MQ=255)

               aCATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGtt                                                                                 <  2:3113536/100‑1 (MQ=255)

               aCATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGtt                                                                                 <  2:2352992/100‑1 (MQ=255)

               aCATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGtt                                                                                 <  2:913661/100‑1 (MQ=255)

                 aTCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTaa                                                                               >  2:2047563/1‑100 (MQ=255)

                  tCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAAt                                                                              <  1:427586/100‑1 (MQ=255)

                  tCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAAt                                                                              <  2:3032245/100‑1 (MQ=255)

                  tCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAAt                                                                              <  2:3406557/100‑1 (MQ=255)

                   ccTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATa                                                                             >  1:325794/1‑100 (MQ=255)

                    cTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTAAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATAc                                                                            >  1:1901156/1‑100 (MQ=255)

                     ttGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACt                                                                           <  2:3064306/100‑1 (MQ=255)

                     ttGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACt                                                                           <  1:1355173/100‑1 (MQ=255)

                     ttGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACt                                                                           <  1:2979094/100‑1 (MQ=255)

                       ggCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTc                                                                         <  2:1308295/100‑1 (MQ=255)

                        gCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCa                                                                        <  2:3553574/100‑1 (MQ=255)

                        gCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCa                                                                        <  1:3555310/100‑1 (MQ=255)

                          tGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAAc                                                                      >  1:2504937/1‑100 (MQ=255)

                          tGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAAc                                                                      <  2:1980838/100‑1 (MQ=255)

                           gCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACt                                                                     >  1:1324786/1‑100 (MQ=255)

                            cAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACtt                                                                    >  2:142914/1‑100 (MQ=255)

                             aGGTTTAGTTGTAAACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTc                                                                   <  2:2303068/100‑1 (MQ=255)

                              ggTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCt                                                                  >  1:430611/1‑100 (MQ=255)

                              ggTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCt                                                                  >  1:611578/1‑100 (MQ=255)

                              ggTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCt                                                                  >  1:613652/1‑100 (MQ=255)

                              ggTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCt                                                                  >  1:1002946/1‑100 (MQ=255)

                               gTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTg                                                                 >  2:2800530/1‑100 (MQ=255)

                                 ttAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGt                                                               <  2:1100330/100‑1 (MQ=255)

                                   aGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTg                                                             <  2:1664385/100‑1 (MQ=255)

                                    gTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGc                                                            >  1:225524/1‑100 (MQ=255)

                                      tGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCAt                                                          >  2:1898946/1‑100 (MQ=255)

                                      tGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCAt                                                          >  2:186206/1‑100 (MQ=255)

                                      tGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCAt                                                          >  2:1696244/1‑100 (MQ=255)

                                      tGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCAt                                                          >  1:3059253/1‑100 (MQ=255)

                                      tGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCAt                                                          >  1:2746314/1‑100 (MQ=255)

                                      tGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCAt                                                          >  2:2496523/1‑100 (MQ=255)

                                       gTATACCTCTCATAAATTTAAAATTATTTCAATGTGTTCAATAAATCTAGAACAAATTAGCTGATTATGATGGGTTAATACTTCAACTTCTGGTTGCATg                                                         <  1:3160120/100‑1 (MQ=255)

                                          cgccacTCCTAGATTTAAAGAATTGGCAATGTGTTCAATAACGCTCGAACACATTCGCTCATTATAATCAGTTAATACTTCAACTTCTGGTTGCATGAtt                                                      >  1:2961979/3‑100 (MQ=255)

                                           accacTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGAttg                                                     <  1:1688372/100‑1 (MQ=255)

                                           accacTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGAttg                                                     <  2:3556644/100‑1 (MQ=255)

                                              acTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGAttgttt                                                  <  2:3244558/100‑1 (MQ=255)

                                               cTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGAttgtttg                                                 >  1:441834/1‑100 (MQ=255)

                                                  cTATATTTAATATGTTGGCGATGTGTTCAGTAAAGCTCGAACCAATCGGCTCATTATGCTCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTcc                                              <  1:1645211/100‑1 (MQ=255)

                                                  cTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTcc                                              <  2:896829/100‑1 (MQ=255)

                                                   tAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCg                                             <  2:2094998/100‑1 (MQ=255)

                                                   tAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTAGCATGATTGTTTGTCCg                                             >  2:700113/1‑100 (MQ=255)

                                                   tAAATTTAATGTGTTGGCAATGTATTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCg                                             <  1:1797653/100‑1 (MQ=255)

                                                      aTTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTaa                                          >  1:2352868/1‑100 (MQ=255)

                                                          aaTGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAg                                      >  1:3043917/1‑100 (MQ=255)

                                                          aaTGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAg                                      <  2:1597449/100‑1 (MQ=255)

                                                          aaTGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAg                                      <  1:904319/100‑1 (MQ=255)

                                                          aaTGTGTTGGCAATGTGTGCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTGCTGGGTGCATGATTGTTTGTCCGTAAAAAg                                      <  1:2207426/100‑1 (MQ=255)

                                                              tgtTGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATaa                                  >  1:461669/1‑100 (MQ=255)

                                                                ttGGCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAACACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAAcg                                <  2:2604041/100‑1 (MQ=255)

                                                                  ggCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAAcgcg                              <  2:1065874/100‑1 (MQ=255)

                                                                  ggCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAAcgcg                              <  1:2447600/100‑1 (MQ=255)

                                                                  ggCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAAcgcg                              <  2:1944167/100‑1 (MQ=255)

                                                                  ggCAATGTGTTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAAcgcg                              >  2:2250597/1‑100 (MQ=255)

                                                                     aaTGTGTTCAATAAAGTTTGAACAAATTCGTTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATACCGCGcc                            <  1:2913702/99‑1 (MQ=255)

                                                                         tgtTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCg                       >  1:2577256/1‑100 (MQ=255)

                                                                          gtTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCgg                      >  2:2220104/1‑100 (MQ=255)

                                                                          gtTCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCgg                      <  2:1014180/100‑1 (MQ=255)

                                                                            tCAATAAAGCTCGAACAAATTCTCTCAGTATGATCGGGTAATACTTCAACTTCTGGTTGCATGATTGATGGTCCGTAAAAAGATAACGCGCCTGccttg                     >  1:2117792/1‑96 (MQ=255)

                                                                            tCAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGgt                    <  1:2036375/100‑1 (MQ=255)

                                                                            tCAATAAAGCTCGAACAAATTAGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGgt                    >  2:1426648/1‑100 (MQ=255)

                                                                             cAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGgta                   >  2:1913227/1‑100 (MQ=255)

                                                                             cAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGgta                   >  1:1753382/1‑100 (MQ=255)

                                                                             cAATAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGgta                   >  2:2690920/1‑100 (MQ=255)

                                                                              aaTAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGgtag                  <  1:155830/100‑1 (MQ=255)

                                                                                tAAAGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGgtagta                <  2:531888/100‑1 (MQ=255)

                                                                                 aaaGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCTGGGtagtag               <  1:1505038/100‑1 (MQ=255)

                                                                                 aaaGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGtagtag               <  2:1733180/100‑1 (MQ=255)

                                                                                 aaaGCTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGtagtag               <  1:2237176/100‑1 (MQ=255)

                                                                                     cTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAgg           >  1:491023/1‑100 (MQ=255)

                                                                                     cTCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAgg           >  1:1281044/1‑100 (MQ=255)

                                                                                      tCGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGgc          <  1:2928913/100‑1 (MQ=255)

                                                                                       cGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCTGTAAAAAGATAACGCGCCTGCCGGGCAGTAGCa             >  1:3303837/1‑96 (MQ=255)

                                                                                       cGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGgcg         <  1:729302/100‑1 (MQ=255)

                                                                                       cGAACAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGgcg         >  1:3556688/1‑100 (MQ=255)

                                                                                          aCAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCAt      <  1:983951/100‑1 (MQ=255)

                                                                                           cAAATTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCAtt     >  2:3080326/1‑100 (MQ=255)

                                                                                            aaaTTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTa    <  1:2336754/100‑1 (MQ=255)

                                                                                             aaTTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTAc   <  2:2892257/100‑1 (MQ=255)

                                                                                              aTTCGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGAGCCTGCCGGGTAGTAGCAGGCGCATTACg  >  1:1459880/1‑100 (MQ=255)

GGCTTCGCTCACTATACATCCTTGGCTGCAGGTTTAGTTGTACACCACTCCTAAATTTAATGTGTTGGCAATGTGTTCAATAAAGCTCGAACAAATTAGCTCATTATGATCGGTTAATACTTCAACTTCTGGTTGCATGATTGTTTGTCCGTAAAAAGATAACGCGCCTGCCGGGTAGTAGCAGGCGCATTACG  >  NC_000913/263075‑263268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`