Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9840TG49.4% 203.1 / inf 341intergenic (‑194/+114)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base T (76/96);  new base G (153/15);  total (230/111)
Fisher's exact test for biased strand distribution p-value = 2.33e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.93e-02

Only 100 of 345 total aligned reads displayed.

TTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCT  >  NC_000913/3705885‑3706083
                                                                                                   |                                                                                                            
ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                                              <  2:2409021/100‑1 (MQ=255)
     gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                                                         >  2:1721599/1‑100 (MQ=255)
       cGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCgg                                                                                                       <  2:1545906/100‑1 (MQ=37)
         gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                                     <  2:324655/100‑1 (MQ=255)
            ggCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGCATaa                                                                                                  <  1:1231461/100‑2 (MQ=25)
              cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTAGGCCGGATAAGGCGTTAACGCCGCATCCGGcataaac                                                                                                <  1:2640127/100‑7 (MQ=21)
                 tacACGCAGAGGCGCAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAAt                                                                                            >  1:2614554/3‑100 (MQ=255)
                  gCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATc                                                                                           <  1:3747369/100‑1 (MQ=255)
                   cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                                          <  1:3380800/100‑1 (MQ=255)
                     cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                                        >  2:1017670/1‑100 (MQ=255)
                      gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                       <  1:2408183/100‑1 (MQ=255)
                      gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                       <  2:1971413/100‑1 (MQ=255)
                      gCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                       <  2:3743766/100‑1 (MQ=255)
                       cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                                      >  1:1674624/1‑100 (MQ=255)
                           ggCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt                                                                                  >  1:1093213/1‑100 (MQ=255)
                             cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                                >  1:2468664/1‑100 (MQ=255)
                               caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                                              <  1:1954244/100‑1 (MQ=255)
                                    ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                                         >  1:3473205/1‑100 (MQ=255)
                                    ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                                         <  1:3172799/100‑1 (MQ=255)
                                    ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                                         <  1:1326881/100‑1 (MQ=255)
                                     cACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTg                                                                        >  2:3603274/1‑100 (MQ=255)
                                       ccGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCa                                                                      >  1:429467/1‑100 (MQ=255)
                                        cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg                                                                    <  2:3698067/100‑1 (MQ=255)
                                          ggTATACAGATTGTTGACAAAGGGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGaa                                                                 >  2:2254631/1‑100 (MQ=255)
                                              taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAtt‑‑‑tt                                                         >  1:3019903/1‑100 (MQ=255)
                                                cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑g                                                     <  1:1017674/100‑1 (MQ=255)
                                                    ttgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTACAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAgg                                                 >  2:2447239/1‑100 (MQ=255)
                                                     tgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGc                                                <  1:503812/100‑1 (MQ=255)
                                                      tcttccgatctGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGcc                                               <  1:1885367/90‑1 (MQ=255)
                                                             aaGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAg                                        <  2:1430235/100‑1 (MQ=255)
                                                               gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCg                                      <  2:249966/100‑1 (MQ=255)
                                                                 gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCAGAtcggaaga                                    >  2:1885358/1‑93 (MQ=18)
                                                                   gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgc                                  <  2:3361887/100‑1 (MQ=255)
                                                                      ttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCa                               <  1:843644/100‑1 (MQ=255)
                                                                       tttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATc                             >  2:2838826/8‑100 (MQ=18)
                                                                       tGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCAt                              >  2:2294694/1‑100 (MQ=255)
                                                                       cttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAGC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCAt                              >  2:59665/8‑100 (MQ=17)
                                                                        ttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGT‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCa                            >  2:1072349/7‑100 (MQ=21)
                                                                        gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATc                             >  1:2772259/1‑100 (MQ=255)
                                                                        gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATc                             >  2:1356618/1‑100 (MQ=255)
                                                                         tttATGCAGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa                            <  2:1937997/100‑1 (MQ=255)
                                                                         cgtaggCCGGATAAGGCGGTCACGCCGCATCCGGCCTACGAAACCGCAAGAATTCAATATATTGCAGGAGC‑‑‑GGT‑‑GTAGGCCTGATAAGCGTAGCGCATCa                            >  1:471476/6‑100 (MQ=18)
                                                                          ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAg                           <  1:1381135/100‑1 (MQ=255)
                                                                          gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCATACAAAAGCGTGCAAATTCAATACATTGCATGGGCC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                           >  2:3571225/5‑100 (MQ=17)
                                                                          gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                           >  1:2372414/5‑100 (MQ=18)
                                                                           taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAGAAAAACCTGCAAATTCAATATATTGCAGGATA‑‑‑CGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                          >  1:2739510/4‑100 (MQ=18)
                                                                           taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATATTGCCAATTCAATATATTGCAGGACC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                          >  2:694987/4‑100 (MQ=18)
                                                                           taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                          >  1:1533441/4‑100 (MQ=21)
                                                                           tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAgg                          <  2:2699527/100‑1 (MQ=255)
                                                                            aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                        >  1:601315/3‑100 (MQ=18)
                                                                            aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                        >  1:1768886/3‑100 (MQ=18)
                                                                            aggCCGGATAAGGCGTTTACGCCGCATCCGGCATACAAAATCGTGCAAATTCAACATATTGCAATT‑‑‑CTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGc                         >  2:218065/3‑100 (MQ=18)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGATCGATC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                         >  2:2569137/3‑100 (MQ=21)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                         >  2:3308462/3‑100 (MQ=21)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGC‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                         >  1:2201857/3‑100 (MQ=18)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGC‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                         >  2:298374/3‑100 (MQ=18)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGAT‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                         >  1:1300363/3‑100 (MQ=17)
                                                                            aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc                         >  1:2714300/1‑100 (MQ=255)
                                                                            aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc                         >  1:1572347/1‑100 (MQ=255)
                                                                            aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc                         >  1:945137/1‑100 (MQ=255)
                                                                             ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAt                       >  1:234876/2‑100 (MQ=18)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACGAAATCGTGCTAATTCAATATATTGTGGGCTC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                        >  1:2157786/2‑100 (MQ=18)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTACACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                        >  1:685648/2‑100 (MQ=21)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATC‑‑‑TCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCa                        >  1:2517076/2‑100 (MQ=18)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACT‑‑‑AT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCa                        >  2:3655772/2‑100 (MQ=18)
                                                                              gtcggataaggcgTTCACGCCGCATCCGACCCACAAAGTCTTGCAAAATCTATAAATTGCAGGAAC‑‑‑GAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa                       >  2:80913/10‑99 (MQ=21)
                                                                              gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag                       >  1:2074816/1‑99 (MQ=17)
                                                                              gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTA‑‑‑TTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaa                       >  2:2739823/1‑99 (MQ=25)
                                                                               ccggataaggcgTTTACGCCGCATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTT‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaa                       >  1:3341638/9‑99 (MQ=21)
                                                                               ccggataaggcgTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGC‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                      >  1:3246393/9‑98 (MQ=18)
                                                                               ccggataaggcgTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATC‑‑‑TCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCaat                      >  2:2020292/9‑98 (MQ=18)
                                                                               ccggataaggcgTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtt                      >  1:709399/9‑100 (MQ=18)
                                                                               ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtt                      >  2:3532196/1‑100 (MQ=255)
                                                                               ccGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAttt                     >  2:1614247/1‑100 (MQ=18)
                                                                               ccGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATAT‑‑‑GAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGcgatt                     >  2:889768/1‑96 (MQ=21)
                                                                                cggataaggcgTTCACGCCGCATCCGGCCTACAAAAACATGCAAATTCAATAGATTGCAGAGATT‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaatt                     >  1:3005037/8‑97 (MQ=17)
                                                                                 ggataagtcgTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGC‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                    >  1:2041409/9‑100 (MQ=17)
                                                                                 ggataaggcgTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAAC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                    >  1:3640349/7‑100 (MQ=18)
                                                                                 ggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                    >  1:1463948/7‑100 (MQ=17)
                                                                                 ggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                    >  1:769351/7‑100 (MQ=17)
                                                                                 ggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                    >  1:3422970/7‑100 (MQ=17)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                    >  1:1993758/7‑100 (MQ=18)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                    >  1:2827041/7‑100 (MQ=18)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaattc                    >  2:2392177/7‑96 (MQ=21)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGC‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                    >  1:2305955/7‑100 (MQ=17)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGT‑‑‑TAT‑‑CTAGGCCTGATAAGCGTAGCGCATCAGGCAtttat                   >  2:2053865/7‑98 (MQ=21)
                                                                                  gataaggcgTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTA‑‑‑TTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaatctt                   >  1:3516041/6‑95 (MQ=25)
                                                                                  gataaggcgTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGC‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgtttg                   >  2:1181113/6‑99 (MQ=18)
                                                                                  gataaggcgTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGC‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgtttg                   >  2:164637/6‑99 (MQ=18)
                                                                                  gATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAttttt                   <  2:521095/100‑1 (MQ=255)
                                                                                   aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg                  <  1:1150871/100‑1 (MQ=255)
                                                                                    taaggcgTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAAC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAGTtttgc                 >  2:462767/4‑100 (MQ=18)
                                                                                     gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc                <  2:429465/100‑1 (MQ=255)
                                                                                      cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct               >  1:2862474/1‑100 (MQ=255)
                                                                                      cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct               <  1:958040/100‑1 (MQ=255)
                                                                                       ggcgTTTACGCCGCATCCGGCCTACAAAACAGTGCAAATTCAATATATTGCAATCTT‑‑‑CTG‑‑GTAGGCCTGATAAGCATCGCGCATCAGGCaagcttacatt              <  1:2425370/100‑11 (MQ=25)
                                                                                         cgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttg            <  1:1653586/100‑1 (MQ=255)
                                                                                            tAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCa         <  2:301342/100‑1 (MQ=255)
                                                                                                 ccTTATCCGACCTACAAAATCGTGCAAATTCAATACATTGCAGCATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGt    >  2:317812/1‑100 (MQ=255)
                                                                                                   ttATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCt  <  2:933787/100‑1 (MQ=255)
                                                                                                   |                                                                                                            
TTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCT  >  NC_000913/3705885‑3706083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: