Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,446,3620A.25.0% 341.3 / 119.0 132intergenic (+156/+16)ydbL/feaRDUF1318 family protein/transcriptional activator for tynA and feaB
Reads supporting (aligned to +/- strand):  ref base A (45/54);  new base . (20/13);  total (65/67)
Fisher's exact test for biased strand distribution p-value = 1.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.95e-01

Only 100 of 137 total aligned reads displayed.

CTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAA  >  NC_000913/1446263‑1446461
                                                                                                     |                                                                                                   
cTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACa                                                                                                     >  1:806273/1‑100 (MQ=255)
cTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACa                                                                                                     >  2:363624/1‑100 (MQ=255)
cTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACa                                                                                                     >  2:52563/1‑100 (MQ=255)
 tGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACAt                                                                                                    <  1:1814329/100‑1 (MQ=255)
  gAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATg                                                                                                   <  1:2385598/100‑1 (MQ=255)
   aaaCAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGc                                                                                                  <  1:2101150/100‑1 (MQ=255)
   aaaCAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGc                                                                                                  <  1:3784030/100‑1 (MQ=255)
        aGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTTCAAAATCCATACTCAAATAGCTGTTTTATCTAATAGACGATGCAGtacataca                                                                                                 >  2:767388/1‑96 (MQ=255)
        aGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGc                                                                                             <  2:951158/100‑1 (MQ=38)
        aGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGc                                                                                             <  1:941701/100‑1 (MQ=38)
        aGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGc                                                                                             <  1:74787/100‑1 (MQ=38)
         ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGcc                                                                                            <  2:1474368/100‑1 (MQ=38)
         ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGcc                                                                                            <  1:1539872/100‑1 (MQ=38)
         ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGcc                                                                                            >  1:782502/1‑100 (MQ=255)
         ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGcc                                                                                            <  2:229918/100‑1 (MQ=255)
          gTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCa                                                                                           <  1:2224621/100‑1 (MQ=38)
          gTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCa                                                                                           <  1:350649/100‑1 (MQ=255)
            gAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCAAt                                                                                         <  1:1861483/100‑1 (MQ=38)
             aaTATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCAAtt                                                                                        <  2:3250433/100‑1 (MQ=38)
             aaTATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAtt                                                                                        <  1:2334426/100‑1 (MQ=255)
             aaTATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAtt                                                                                        <  1:2565121/100‑1 (MQ=255)
             aaTATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAtt                                                                                        <  2:806271/100‑1 (MQ=255)
              atatTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAtta                                                                                       <  2:3549618/100‑1 (MQ=255)
               natTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAttag                                                                                      >  1:352057/2‑100 (MQ=255)
                 ttCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAttagtt                                                                                    <  2:3393799/100‑1 (MQ=255)
                 ttCAAAAACTCCTGTCAAATTGCATTATGAACAGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAttagtt                                                                                    <  2:3528955/100‑1 (MQ=38)
                  tCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAttagtta                                                                                   >  1:3411919/1‑100 (MQ=255)
                   cAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAttagttag                                                                                  >  1:2782880/1‑100 (MQ=255)
                    aaaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAAAGCCAAttagtta                                                                                   >  2:1707637/1‑98 (MQ=255)
                     aaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCg                                                                                <  1:2108639/100‑1 (MQ=255)
                     aaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCg                                                                                >  1:2980395/1‑100 (MQ=255)
                      aaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCgg                                                                               <  2:1984965/100‑1 (MQ=255)
                            cTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTa                                                                         >  1:1478104/1‑100 (MQ=255)
                             tGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATACAACGCCAATTAGTTAGCGGAATTTAc                                                                        >  2:3408300/1‑100 (MQ=255)
                              gTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACg                                                                       <  1:332854/100‑1 (MQ=255)
                              gTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACg                                                                       <  1:3700656/100‑1 (MQ=255)
                              gTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACg                                                                       <  2:737894/100‑1 (MQ=255)
                               tCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGt                                                                      <  1:3718958/100‑1 (MQ=255)
                                 aaaTTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCg                                                                    <  1:1530586/100‑1 (MQ=255)
                                 aaaTTGCCTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGa                                                                   <  1:2303158/100‑1 (MQ=255)
                                   aTTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGAt                                                                  <  2:1158253/100‑1 (MQ=255)
                                      gCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACt                                                               >  1:1991078/1‑100 (MQ=255)
                                       ccTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTc                                                              >  2:2177383/1‑100 (MQ=255)
                                        cTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTACTTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCg                                                             <  2:2862337/100‑1 (MQ=255)
                                        cTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCg                                                             <  2:2823285/100‑1 (MQ=255)
                                          tttGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGcc                                                           <  2:2487474/100‑1 (MQ=255)
                                            tGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCtn                                                         <  2:3815259/100‑2 (MQ=255)
                                            tGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTg                                                         <  2:3660386/100‑1 (MQ=255)
                                               ccTGAAAAATTCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCg                                                      <  2:535937/100‑1 (MQ=255)
                                               ccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCg                                                      >  2:3705187/1‑100 (MQ=255)
                                                cTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGt                                                     >  2:260613/1‑100 (MQ=255)
                                                cTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGt                                                     <  1:3788853/100‑1 (MQ=255)
                                                 tGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTc                                                    >  1:3106281/1‑100 (MQ=255)
                                                    aaaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATc                                                 >  2:359050/1‑100 (MQ=255)
                                                         gCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCaaa                                            >  2:3183917/1‑100 (MQ=255)
                                                           aTAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGc                                          <  2:2746295/100‑1 (MQ=255)
                                                            tAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCg                                         >  1:1622931/1‑100 (MQ=255)
                                                                 aaaTATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGc                                     <  2:2706646/99‑1 (MQ=255)
                                                                       cTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATa                              <  1:2165420/100‑1 (MQ=255)
                                                                         gTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATAcc                            >  2:692161/1‑100 (MQ=255)
                                                                         gTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATAcc                            >  1:1979332/1‑100 (MQ=255)
                                                                          ngctAACTAATTGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCg                           >  1:2198600/4‑100 (MQ=25)
                                                                           gctAACTAATTGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGt                          >  2:1707091/3‑100 (MQ=25)
                                                                            ttAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGtt                         >  1:306986/1‑100 (MQ=255)
                                                                                cTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaa                     <  2:1487194/100‑1 (MQ=255)
                                                                                cTAATTGGCGTTGCAGTACATGCAACGCCAATAAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaa                     >  1:869244/1‑100 (MQ=255)
                                                                                 tAATTGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaaa                    >  1:3752296/1‑100 (MQ=38)
                                                                                  aaTTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaaaa                   >  1:1194026/1‑100 (MQ=255)
                                                                                  aaTTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaaaa                   >  1:324563/1‑100 (MQ=255)
                                                                                  aaTTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaaaa                   >  1:381772/1‑100 (MQ=255)
                                                                                   aTTGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAAt                  >  2:185702/1‑100 (MQ=38)
                                                                                   aTTGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAAt                  >  1:2802442/1‑100 (MQ=38)
                                                                                   aTTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAAt                  >  1:3529815/1‑100 (MQ=255)
                                                                                    ttGGCGTTGCGTGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATg                 >  1:3087707/1‑100 (MQ=38)
                                                                                    ttGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATg                 <  1:369820/100‑1 (MQ=255)
                                                                                     tGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGa                >  1:1820055/1‑100 (MQ=38)
                                                                                     tGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGa                >  1:3156316/1‑100 (MQ=38)
                                                                                     tGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGa                >  1:638124/1‑100 (MQ=38)
                                                                                     tGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGa                >  1:931138/1‑100 (MQ=38)
                                                                                     tGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGa                >  1:674461/1‑100 (MQ=38)
                                                                                     tGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaa                     >  2:1253325/1‑95 (MQ=255)
                                                                                      ggCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc               >  1:100267/1‑100 (MQ=38)
                                                                                      ggCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc               >  1:1241159/1‑100 (MQ=38)
                                                                                      ggCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc               >  1:3175886/1‑100 (MQ=38)
                                                                                      ggCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc               >  2:1189871/1‑100 (MQ=38)
                                                                                      ggCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc               >  2:2517887/1‑100 (MQ=38)
                                                                                      ggCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc               >  2:3845001/1‑100 (MQ=255)
                                                                                      ggCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc               <  2:1979318/100‑1 (MQ=255)
                                                                                       gCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAct              >  2:1047457/1‑100 (MQ=38)
                                                                                           tGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTgg          <  2:3028601/100‑1 (MQ=255)
                                                                                           tGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTgg          >  2:184883/1‑100 (MQ=255)
                                                                                           tGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTgg          >  1:1409126/1‑100 (MQ=255)
                                                                                           tGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTgg          <  2:782500/100‑1 (MQ=255)
                                                                                            gCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACTTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGTCTCTGGt         >  2:2082154/1‑100 (MQ=255)
                                                                                            gCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGt         >  2:2353921/1‑100 (MQ=255)
                                                                                               aGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCa       >  2:3210812/1‑100 (MQ=255)
                                                                                               aGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCa       <  2:1651594/100‑1 (MQ=255)
                                                                                               aGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAAAGACTCTg           >  2:1003314/1‑96 (MQ=255)
                                                                                                 gTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAg      >  2:1786549/1‑100 (MQ=255)
                                                                                                     aTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGaaaa  <  1:1408836/100‑1 (MQ=255)
                                                                                                     |                                                                                                   
CTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAA  >  NC_000913/1446263‑1446461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: