BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,378,929	0	A	G	39.2%	96.9 / 163.0	148	S357P (TCA→CCA)	zapE	divisome ATPase
Reads supporting (aligned to +/- strand): ref base A (41/49); new base G (26/32); total (67/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.46e-01

Only 100 of 148 total aligned reads displayed.

GGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAA  >  NC_000913/3378831‑3379027

ggTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGAc                                                                                                   >  2:3583917/1‑100 (MQ=255)

 gTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGGCa                                                                                                  <  1:323255/100‑1 (MQ=255)

 gTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACa                                                                                                  <  1:3183189/100‑1 (MQ=255)

  tCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAg                                                                                                 <  2:1129171/100‑1 (MQ=255)

   cAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAgg                                                                                                >  2:2169112/1‑100 (MQ=255)

     aaGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCa                                                                                              >  1:1179827/1‑100 (MQ=255)

       gATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAAc                                                                                            <  1:1600357/100‑1 (MQ=255)

         tCGACCCCTTTTTGTGATTTGTGACAGTTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGc                                                                                          <  2:791496/100‑1 (MQ=255)

           gACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGGCAGGCAACGCTg                                                                                        <  1:1874213/100‑1 (MQ=255)

           gACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGGCAGGCAACGCTg                                                                                        <  2:2852401/100‑1 (MQ=255)

            aCCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGGCAGGCAACGCTgg                                                                                       <  2:2067005/100‑1 (MQ=255)

            aCCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTgg                                                                                       <  1:153728/100‑1 (MQ=255)

             ccccTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCCCTTGCAGACGTGGCAGGCAACGCTGGa                                                                                      <  1:1450675/100‑1 (MQ=255)

             ccccTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCACTTTGCATCTCTTGCAGACGTGACAGGCAACGCTgg                                                                                       <  1:2731016/99‑1 (MQ=255)

               ccTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCCTCTCTTGCAGACGTGACAGGCAACGCTGGAAc                                                                                    >  2:1668150/1‑100 (MQ=255)

               ccTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGGCAGGCAACGCTGGAAc                                                                                    <  2:2987775/100‑1 (MQ=255)

               ccTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAAc                                                                                    >  2:2318017/1‑100 (MQ=255)

                cTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGGCAGGCAACGCTGGAACt                                                                                   >  2:967987/1‑100 (MQ=255)

                    ttgtgatttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCaaa                                                                               >  2:193427/1‑100 (MQ=255)

                     tgtgatttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGGCAGGCAACGCTGGAACTCAAAc                                                                              <  2:1751049/100‑1 (MQ=255)

                     tgtgatttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGGCAGGCAACGCTGGAACTCAAAc                                                                              <  1:3324799/100‑1 (MQ=255)

                     tgtgatttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGACACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAAc                                                                              <  2:1820813/100‑1 (MQ=255)

                        gatttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTc                                                                           >  1:3415165/1‑100 (MQ=255)

                        gatttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTc                                                                           <  1:3601609/100‑1 (MQ=255)

                         atttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCa                                                                          >  1:3449326/1‑100 (MQ=255)

                          tttgtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAg                                                                         >  2:435787/1‑100 (MQ=255)

                             gtgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCg                                                                      >  1:1423536/1‑100 (MQ=255)

                              tgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGTATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGa                                                                     >  1:3657065/1‑100 (MQ=255)

                              tgaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGa                                                                     >  2:3392892/1‑100 (MQ=255)

                               gaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGAt                                                                    >  1:2795487/1‑100 (MQ=255)

                               gaCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGAt                                                                    >  1:258870/1‑100 (MQ=255)

                                aCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATc                                                                   <  2:1584318/100‑1 (MQ=255)

                                aCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGTCGATc                                                                   >  2:941766/1‑100 (MQ=255)

                                aCAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATc                                                                   <  2:48075/100‑1 (MQ=255)

                                 cAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCg                                                                  <  1:2469057/100‑1 (MQ=255)

                                 cAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCg                                                                  >  1:3854074/1‑100 (MQ=255)

                                 cAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCg                                                                  >  1:1010613/1‑100 (MQ=255)

                                  aGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGc                                                                 <  2:3415135/100‑1 (MQ=255)

                                   ggTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGcc                                                                >  2:2771685/1‑100 (MQ=255)

                                     ttttAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGcc                                                                >  2:433628/1‑98 (MQ=255)

                                     ttttAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCt                                                              >  1:2987703/1‑100 (MQ=255)

                                     ttttAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCt                                                              >  1:1880034/1‑100 (MQ=255)

                                      tttAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTg                                                             <  1:2280911/100‑1 (MQ=255)

                                       ttAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGa                                                            <  1:1871376/100‑1 (MQ=255)

                                        tAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGAt                                                           >  1:1047935/1‑100 (MQ=255)

                                         aaCCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATa                                                          >  1:941857/1‑100 (MQ=255)

                                           cccGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATaaa                                                        >  1:3283228/1‑100 (MQ=255)

                                            ccGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAAt                                                       >  1:63370/1‑100 (MQ=255)

                                             cGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAAtt                                                      <  2:3660356/100‑1 (MQ=255)

                                             cGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAAtt                                                      <  1:2565781/100‑1 (MQ=255)

                                               ccAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGGTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTc                                                    <  1:606635/100‑1 (MQ=255)

                                               ccAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACGCAAACTTCAGCCGATCGCCCTGATAAATTTc                                                    <  2:2371233/100‑1 (MQ=255)

                                                cAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCa                                                   <  1:3105094/100‑1 (MQ=255)

                                                 aaaTGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCAt                                                  <  1:2131168/100‑1 (MQ=255)

                                                   aTGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATAc                                                <  2:3795739/100‑1 (MQ=255)

                                                      cTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGc                                             >  2:2955982/1‑100 (MQ=255)

                                                        cgcgCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCgg                                           >  1:1151052/1‑100 (MQ=255)

                                                         gcgcTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGc                                          <  1:165913/100‑1 (MQ=255)

                                                          cgcTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCa                                         >  1:2868279/1‑100 (MQ=255)

                                                            cTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACt                                       >  1:266135/1‑100 (MQ=255)

                                                              tCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTc                                     <  2:1010609/100‑1 (MQ=255)

                                                               cAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCt                                    >  2:3073474/1‑100 (MQ=255)

                                                                 gATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGc                                  <  2:1492208/100‑1 (MQ=255)

                                                                     ctctTCGCTTTGCATCTCTTGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTc                              <  2:1463514/100‑1 (MQ=255)

                                                                     ctctTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTc                              <  2:3852037/100‑1 (MQ=255)

                                                                     ctctTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTc                              <  1:2169130/100‑1 (MQ=255)

                                                                     ctctTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTc                              <  1:3583949/100‑1 (MQ=255)

                                                                       ctTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTcac                            >  1:878366/1‑100 (MQ=255)

                                                                        ttCGCTTTGCATCTCTTGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCTCTGATAAATTTCATACAGCGGCACTTCTGCACTcacc                           <  2:306941/100‑1 (MQ=255)

                                                                        ttCGCTTTGCATCTCTTGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTcacc                           <  2:668577/100‑1 (MQ=255)

                                                                        ttCGCTTTGCATCTCTTGCAGACGTGACTGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTcacc                           <  1:3660491/100‑1 (MQ=255)

                                                                            cTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTa                       >  1:2823967/1‑100 (MQ=255)

                                                                            cTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTa                       >  1:2200717/1‑100 (MQ=255)

                                                                             tttGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCGAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTaa                      <  2:636010/100‑1 (MQ=255)

                                                                               tGCATCTCTTGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAAtt                    <  1:2708108/100‑1 (MQ=255)

                                                                               tGCATCTCTTGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAAtt                    >  1:2460598/1‑100 (MQ=255)

                                                                                gCATCTCTTGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAAttt                   <  1:842298/100‑1 (MQ=255)

                                                                                 cATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTg                  <  2:825536/100‑1 (MQ=255)

                                                                                 cATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTg                  <  2:2651457/100‑1 (MQ=255)

                                                                                 cATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTg                  <  2:1205599/100‑1 (MQ=255)

                                                                                  aTCTCTTGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGa                 >  2:2632494/1‑100 (MQ=255)

                                                                                   tctcTTGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGAc                >  2:213847/1‑100 (MQ=255)

                                                                                    ctctTGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACa               <  2:266132/100‑1 (MQ=255)

                                                                                      ctTGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATg             <  2:3253022/100‑1 (MQ=255)

                                                                                      ctTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATg             >  1:470682/1‑100 (MQ=255)

                                                                                       ttGCAGACGTGACAGGAAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATgg            <  1:3095334/100‑1 (MQ=255)

                                                                                        tGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGgc           >  1:2131390/1‑100 (MQ=255)

                                                                                        tGCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGgc           >  2:539876/1‑100 (MQ=255)

                                                                                        tGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGgc           <  2:2820119/100‑1 (MQ=255)

                                                                                         gCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGgcg          <  1:3035162/100‑1 (MQ=255)

                                                                                         gCAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGgcg          >  2:1348137/1‑100 (MQ=255)

                                                                                          cAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGgcgc         >  1:2278290/1‑100 (MQ=255)

                                                                                          cAGACGTGGCAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGgcgc         >  1:3470042/1‑100 (MQ=255)

                                                                                          cAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGgcgc         >  1:1571203/1‑100 (MQ=255)

                                                                                          cAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGgcgc         <  1:1369751/100‑1 (MQ=255)

                                                                                            gACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTc       >  1:1937945/1‑100 (MQ=255)

                                                                                            gACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTc       >  1:2905881/1‑100 (MQ=255)

                                                                                              cGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGt     <  2:2319161/100‑1 (MQ=255)

                                                                                              cGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGt     <  1:3487239/100‑1 (MQ=255)

                                                                                                tGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTaa   <  2:3262441/100‑1 (MQ=255)

GGTCAAAGATCGACCCCTTTTTGTGATTTGTGACAGGTTTTAACCCGCCAAATGCTCGCGCTTCAGATACTCTTCGCTTTGCATCTCTTGCAGACGTGACAGGCAACGCTGGAACTCAAACTTCAGCCGATCGCCCTGATAAATTTCATACAGCGGCACTTCTGCACTCACCACTAATTTGACATGGCGCTCGTAAA  >  NC_000913/3378831‑3379027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`