BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,684,604	G→A	Q339* (CAG→TAG)	yhjK ←	cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,684,604	0	G	A	100.0%	495.1 / NA	142	Q339* (CAG→TAG)	yhjK	cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (72/70); total (72/70)

Only 100 of 142 total aligned reads displayed.

AGATCGCCGTAGAACATCGCCACGCCAATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTGACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGAGGATCATACGTGGCGACA  >  NC_000913/3684507‑3684699

agaTCGCCGTAGAACATCGCCACGCCAATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAAc                                                                                               <  1:3068386/100‑1 (MQ=255)

 gaTCGCCGTAGAACATCGCCATGCCAATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAAcc                                                                                              >  2:586901/1‑100 (MQ=255)

 gaTCGCCGTAGAACATCGCCACGCCAATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAAcc                                                                                              >  1:3735568/1‑100 (MQ=255)

 gaTCGCCGTAGAACATCGCCACGCCAATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAAcc                                                                                              <  1:3030980/100‑1 (MQ=255)

  aTCGCCGTAGAACATCGCCACGCCAATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCt                                                                                             <  2:3249385/100‑1 (MQ=255)

    cGCCGTAGAACATCGCCACGCCAATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTaa                                                                                           >  1:2667231/1‑100 (MQ=255)

    cGCCGTAGAACATCGCCACGCCAATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTaa                                                                                           >  1:466326/1‑100 (MQ=255)

     gCCGTAGAACATCGCCACGCCAATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAg                                                                                          >  1:2984160/1‑100 (MQ=255)

     gCCGTAGAACATCGCCACGCCAATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAg                                                                                          <  2:2544468/100‑1 (MQ=255)

     gCCGTAGAACATCGCCACGCCAATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAg                                                                                          <  2:2679667/100‑1 (MQ=255)

      ccGTAGAACATCGCCACGCCAATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAgg                                                                                         >  1:2765690/1‑100 (MQ=255)

      ccGTAGAACATCGCCACGCCAATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAgg                                                                                         <  2:1428053/100‑1 (MQ=255)

         tAGAACATCGCCACGCCAATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGa                                                                                      >  2:3858979/1‑100 (MQ=255)

          aGAACATCGCCACGCCAATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGAt                                                                                     >  2:3380535/1‑100 (MQ=255)

          aGAACATCGCCACGCCAATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGAt                                                                                     >  1:152825/1‑100 (MQ=255)

          aGAACATCGCCACGCCAATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGAt                                                                                     >  1:3528540/1‑100 (MQ=255)

           gAACATCGCCACGCCAATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGAtt                                                                                    >  1:951053/1‑100 (MQ=255)

           gAACATCGCCACGCCAATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGAtt                                                                                    >  1:242107/1‑100 (MQ=255)

           gAACATCGCCACGCCAATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGAtt                                                                                    >  1:2434718/1‑100 (MQ=255)

            aaCATCGCCACGCCAATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTg                                                                                   <  1:2537772/100‑1 (MQ=255)

             aCATCGCCACGCCAATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGc                                                                                  <  1:202066/100‑1 (MQ=255)

                 cgccacgccaATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGc                                                                              >  1:3714505/1‑100 (MQ=255)

                 cgccacgccaATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGc                                                                              <  1:102474/100‑1 (MQ=255)

                 cgccacgccaATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGc                                                                              <  1:1687805/100‑1 (MQ=255)

                  gccacgccaATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGcc                                                                             >  1:3030011/1‑100 (MQ=255)

                  gccacgccaATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGcc                                                                             >  2:2917628/1‑100 (MQ=255)

                  gccacgccaATGCTACAGTGCGGACGGAGTTGAATACGTCCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTg                                                                               >  2:2428689/1‑98 (MQ=255)

                   ccacgccaATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCa                                                                            >  1:3535860/1‑100 (MQ=255)

                      cgccaATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGTCACgg                                                                         >  1:1600017/1‑100 (MQ=255)

                       gccaATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCTCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCa                                                                            <  2:3535828/96‑1 (MQ=255)

                          aaTGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTcc                                                                     <  2:2109265/100‑1 (MQ=255)

                          aaTGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTcc                                                                     <  2:54180/100‑1 (MQ=255)

                          aaTGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTcc                                                                     <  2:838033/100‑1 (MQ=255)

                          aaTGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTcc                                                                     <  1:2145630/100‑1 (MQ=255)

                          aaTGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACCTGCTAACCTAAGGTGATTGCGTGCCACGGTTcc                                                                     <  2:1417820/100‑1 (MQ=255)

                            tGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTg                                                                   <  1:3382102/100‑1 (MQ=255)

                            tGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTg                                                                   <  2:2788978/100‑1 (MQ=255)

                            tGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTg                                                                   <  2:1099702/100‑1 (MQ=255)

                            tGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTg                                                                   <  1:304957/100‑1 (MQ=255)

                              cTACAGTGCGGACGGAGTTGAAGACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTa                                                                 >  2:2254291/1‑100 (MQ=255)

                               tACAGTGGGGACGCAGTGGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTac                                                                <  2:1583891/100‑1 (MQ=255)

                               tACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTac                                                                <  1:1015108/100‑1 (MQ=255)

                                 cAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTacac                                                              >  2:389597/1‑100 (MQ=255)

                                   gTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCg                                                            >  2:2194417/1‑100 (MQ=255)

                                     gCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGtt                                                          <  2:3528508/100‑1 (MQ=255)

                                      cggacggaGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTg                                                         <  1:2222128/100‑1 (MQ=255)

                                      cggacggaGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTg                                                         <  1:586221/100‑1 (MQ=255)

                                      cggacggaGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTg                                                         >  1:3534898/1‑100 (MQ=255)

                                      cggacggaGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCATGCCACGGTTCCTGTACACCGTTg                                                         <  2:1503293/100‑1 (MQ=255)

                                        gacggaGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGc                                                       <  1:1136320/100‑1 (MQ=255)

                                        gacggaGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGc                                                       <  2:1137344/100‑1 (MQ=255)

                                        gacggaGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGc                                                       <  1:3859014/100‑1 (MQ=255)

                                         acggaGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCa                                                      <  1:3235539/100‑1 (MQ=255)

                                          ccgaGTGGAATACGTGCAAGCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGAGTGCGTGCCACGGTTCCTGTACAACGTTGGCa                                                      <  1:1186512/97‑1 (MQ=255)

                                            gaGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTTCGTGCCACGGTTCCTGTACACCGTTGGCAATg                                                   <  1:3680401/100‑1 (MQ=255)

                                            gaGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATg                                                   <  1:1889772/100‑1 (MQ=255)

                                            gaGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATg                                                   <  2:1748055/100‑1 (MQ=255)

                                            gaGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATg                                                   <  2:2956056/100‑1 (MQ=255)

                                                tGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAg                                               <  1:3058963/100‑1 (MQ=255)

                                                tGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAg                                               >  2:3413869/1‑100 (MQ=255)

                                                tGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAg                                               <  1:2094817/100‑1 (MQ=255)

                                                tGAATACGTGCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGGGATTGCGTGCCACGGTTCCTGTACACCGTGAGCAATGACa                                                >  2:1065388/1‑99 (MQ=255)

                                                    tACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCaaa                                           <  1:3608914/100‑1 (MQ=255)

                                                      ngTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGt                                         >  1:884509/2‑100 (MQ=255)

                                                      cGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGt                                         >  2:118818/1‑100 (MQ=255)

                                                       gTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTc                                        <  1:472006/100‑1 (MQ=255)

                                                       gTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTc                                        >  1:608939/1‑100 (MQ=255)

                                                        ttCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTcn                                       <  2:1704782/100‑2 (MQ=255)

                                                          cAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATa                                     >  2:1211492/1‑100 (MQ=255)

                                                          cAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATa                                     >  1:3090045/1‑100 (MQ=255)

                                                            aTCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAAc                                   <  1:389599/100‑1 (MQ=255)

                                                             ttgtcAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTTTACACCGTTGGCAATGACAGCAAAGTCATAAcc                                  <  2:3290205/96‑1 (MQ=255)

                                                             tCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAAcc                                  <  1:1765296/100‑1 (MQ=255)

                                                                 cAGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTa                              >  1:1205154/1‑100 (MQ=255)

                                                                  aGGCGCTCGCTCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTaa                             >  2:3259986/1‑100 (MQ=255)

                                                                     cgctcgctCATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCt                          >  2:259904/1‑100 (MQ=255)

                                                                         cgctcATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTgcgc                      >  1:1380092/1‑100 (MQ=255)

                                                                           ctcATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGa                    >  2:1810643/1‑100 (MQ=255)

                                                                            tcATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGAg                   <  1:3246796/100‑1 (MQ=255)

                                                                            tcATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGAg                   <  2:152822/100‑1 (MQ=255)

                                                                             cATGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGAgg                  >  1:3591085/1‑100 (MQ=255)

                                                                               tGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGAGGAt                <  2:3044805/100‑1 (MQ=255)

                                                                               tGATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGAGGAt                >  1:26825/1‑100 (MQ=255)

                                                                                gATAGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGAGGATc               <  2:2346629/100‑1 (MQ=255)

                                                                                  tattGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTACTCTGCGCGAGGATCAt             >  2:2131383/4‑100 (MQ=255)

                                                                                   aGTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGAGGATCATa            <  1:3413899/100‑1 (MQ=255)

                                                                                    gTGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGAGGATCATAc           >  2:1749288/1‑100 (MQ=255)

                                                                                     tGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGAGGATCATACg          >  2:3201596/1‑100 (MQ=255)

                                                                                     tGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGAGGATCATACg          >  2:2639347/1‑100 (MQ=255)

                                                                                     tGAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGAGGATCATACg          <  1:2776864/100‑1 (MQ=255)

                                                                                      gAGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGAGGATCATACGt         >  1:1539541/1‑100 (MQ=255)

                                                                                       aGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGAGGATCATACGTg        >  1:20368/1‑100 (MQ=255)

                                                                                       aGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGAGGATCATACGTg        >  2:237643/1‑100 (MQ=255)

                                                                                       aGCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGAGGATCATACGTg        <  2:1765229/100‑1 (MQ=255)

                                                                                        gCACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGAGGATCATACGTgg       <  2:3601991/100‑1 (MQ=255)

                                                                                         cACTTGCTAACCTAAGGTGATTGCGTGCCTCGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGAGGATCATACGTGGc      <  2:1762110/100‑1 (MQ=255)

                                                                                         cACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGAGGATCATACGTGGc      >  2:1658900/1‑100 (MQ=255)

                                                                                         cACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGAGGATCATACGTGGc      <  2:656058/100‑1 (MQ=255)

                                                                                         cACTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGAGGATCATACGTGGc      >  1:3252358/1‑100 (MQ=255)

                                                                                           cTTGCTAACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGAGGATCATACGTGGCGa    >  1:930074/1‑100 (MQ=255)

AGATCGCCGTAGAACATCGCCACGCCAATGCTACAGTGCGGACGGAGTTGAATACGTTCAATCGGCAGGCGCTCGCTCATGATAGTGAGCACTTGCTGACCTAAGGTGATTGCGTGCCACGGTTCCTGTACACCGTTGGCAATGACAGCAAAGTCATAACCGCTAATCTGCGCGAGGATCATACGTGGCGACA  >  NC_000913/3684507‑3684699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`