BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,248,023	G→A	W84* (TGG→TAG)	yqjA →	general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,248,023	0	G	A	100.0%	413.8 / NA	121	W84* (TGG→TAG)	yqjA	general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (70/51); total (70/51)

Only 100 of 126 total aligned reads displayed.

AGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACCA  >  NC_000913/3247926‑3248119

aGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTagg                                                                                                <  2:762830/100‑1 (MQ=255)

  tttACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTc                                                                                              <  1:437471/100‑1 (MQ=255)

  tttACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGACTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTc                                                                                              >  1:2828418/1‑100 (MQ=255)

    tACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAg                                                                                            <  1:3644121/100‑1 (MQ=255)

       tGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCta                                                                                         >  2:3033001/1‑100 (MQ=255)

        ggTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCtat                                                                                        >  2:1480187/1‑100 (MQ=255)

        ggTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCtat                                                                                        >  1:3793071/1‑100 (MQ=255)

          tATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTatat                                                                                      <  1:2907960/100‑1 (MQ=255)

           aTTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATAtt                                                                                     >  1:2392282/1‑100 (MQ=255)

            ttGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTc                                                                                    <  2:3001575/100‑1 (MQ=255)

              ggTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAg                                                                                  >  2:326936/1‑100 (MQ=255)

              ggTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAg                                                                                  >  2:721610/1‑100 (MQ=255)

              ggTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAg                                                                                  >  2:1076030/1‑100 (MQ=255)

               gTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAgg                                                                                 <  1:234831/100‑1 (MQ=255)

               gTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATAGTCAgg                                                                                 <  2:1125678/100‑1 (MQ=255)

                tCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTTTCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGGCAGCTATATTCAggg                                                                                <  2:327938/100‑1 (MQ=255)

                      tgtTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATg                                                                          <  1:476061/100‑1 (MQ=255)

                       gtTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATgg                                                                         >  2:2456595/1‑100 (MQ=255)

                         tGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCt                                                                       <  1:2435232/100‑1 (MQ=255)

                         tGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCt                                                                       >  1:3475319/1‑100 (MQ=255)

                         tGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCt                                                                       >  2:1077422/1‑100 (MQ=255)

                          gATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTg                                                                      >  1:1438201/1‑100 (MQ=255)

                          gATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTg                                                                      >  2:46820/1‑100 (MQ=255)

                           aTTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTgg                                                                     <  2:2435021/100‑1 (MQ=255)

                           aTTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTgg                                                                     >  2:2733906/1‑100 (MQ=255)

                            ttGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTggg                                                                    <  1:1940958/100‑1 (MQ=255)

                             tGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGc                                                                   <  1:764674/100‑1 (MQ=255)

                             tGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGc                                                                   <  2:3809700/100‑1 (MQ=255)

                              gCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCa                                                                  >  2:2569226/1‑100 (MQ=255)

                              gCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCa                                                                  >  1:2108212/1‑100 (MQ=255)

                              gCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCa                                                                  <  2:557848/100‑1 (MQ=255)

                               ccaaaGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCaa                                                                 >  1:2624503/3‑100 (MQ=255)

                               cGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCaa                                                                 >  1:556935/1‑100 (MQ=255)

                               cGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCaa                                                                 >  2:926321/1‑100 (MQ=255)

                               cGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCaa                                                                 >  2:879078/1‑100 (MQ=255)

                                   aGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATAcc                                                             >  1:2704498/1‑100 (MQ=255)

                                    ggCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATAccc                                                            <  2:3793038/100‑1 (MQ=255)

                                    ggCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATAccc                                                            >  1:533047/1‑100 (MQ=255)

                                       gcgATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCa                                                         <  1:2415938/100‑1 (MQ=255)

                                        cgATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCAc                                                        >  1:3837691/1‑100 (MQ=255)

                                           tGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGt                                                     <  1:347782/100‑1 (MQ=255)

                                           tGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGt                                                     >  1:993062/1‑100 (MQ=255)

                                           tGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGt                                                     <  1:2456616/100‑1 (MQ=255)

                                            gggCTATCCGCAAACGCTTCTGCTGCTGTCCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTa                                                    <  2:3507715/100‑1 (MQ=255)

                                             ggCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTAc                                                   <  1:2889008/100‑1 (MQ=255)

                                              gCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACa                                                  <  1:2758678/100‑1 (MQ=255)

                                              gCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACa                                                  >  1:1631291/1‑100 (MQ=255)

                                              gCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACa                                                  >  1:3490713/1‑100 (MQ=255)

                                                tATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACaaa                                                >  1:2537169/1‑100 (MQ=255)

                                                   ccGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACt                                             >  1:2330129/1‑100 (MQ=255)

                                                    cGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTg                                            <  2:3379720/100‑1 (MQ=255)

                                                    cGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTg                                            >  1:3355243/1‑100 (MQ=255)

                                                    cGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTg                                            >  2:1964975/1‑100 (MQ=255)

                                                     gCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTgg                                           <  2:655036/100‑1 (MQ=255)

                                                     gCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTgg                                           >  2:968073/1‑100 (MQ=255)

                                                     gCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTgg                                           >  2:143027/1‑100 (MQ=255)

                                                     gCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTgg                                           <  1:3033028/100‑1 (MQ=255)

                                                      cAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGc                                          <  2:2714831/100‑1 (MQ=255)

                                                       aaaCGATTCTGCTGCTGCCCGTTGCCGCCGGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATCGCTGGGCAATACCCGCACCGTACAAAACTGGCt                                         <  2:2333089/100‑1 (MQ=255)

                                                       aaaCGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCt                                         <  1:1340855/100‑1 (MQ=255)

                                                        aaCGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTa                                        <  2:1631283/100‑1 (MQ=255)

                                                          cGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTAtc                                      >  2:2795878/1‑100 (MQ=255)

                                                          cGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTAt                                       >  2:1455452/1‑99 (MQ=255)

                                                             ttCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCa                                   <  1:1055812/100‑1 (MQ=255)

                                                             ttCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCa                                   <  2:533046/100‑1 (MQ=255)

                                                              tCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCAt                                  >  1:600074/1‑100 (MQ=255)

                                                              tCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCAt                                  <  2:3473735/100‑1 (MQ=255)

                                                              tCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCAt                                  <  2:3020036/100‑1 (MQ=255)

                                                               ctgctgctgACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCAtt                                 <  2:1634149/100‑1 (MQ=255)

                                                               ctgctgctgACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCAtt                                 <  1:415621/100‑1 (MQ=255)

                                                                 gctgctgACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTa                               >  1:419990/1‑100 (MQ=255)

                                                                  ctgctgACCGTTGCCACCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTAc                              >  2:653306/1‑100 (MQ=255)

                                                                    gctgACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTAccc                            >  1:1509946/1‑100 (MQ=255)

                                                                    gctgACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTAccc                            >  1:276649/1‑100 (MQ=255)

                                                                      tgACCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCcgc                          >  1:968402/1‑100 (MQ=255)

                                                                        aCCGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCgcgc                        >  2:1345612/1‑100 (MQ=255)

                                                                         ccGTTGCCGCCAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCcgc                          >  2:3512991/1‑97 (MQ=255)

                                                                         ccGTTGCCGCCAGCCTCGGCGGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCgcgc                        >  2:2366852/1‑99 (MQ=255)

                                                                              gccgccAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatc                  >  1:1260483/1‑100 (MQ=255)

                                                                              gccgccAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGATATCTCATTTACCCGCGCATTatc                  >  1:376828/1‑100 (MQ=255)

                                                                               gatctaGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatca                 <  2:3231904/95‑1 (MQ=255)

                                                                               ccgcgAGCCTCGGCTGGTAGGGCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatca                 <  2:2242239/100‑1 (MQ=255)

                                                                               ccgccAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatca                 >  1:1245350/1‑100 (MQ=255)

                                                                               ccgccAGCCTCGGCTGCTAGCTCAGCTATATTCAGGGCCGATGGCTGTGCCATACCCGCACCGTACAAATCTGGCTATCTCATTTACCCGCGCATTat                   <  2:434139/98‑1 (MQ=255)

                                                                                tagcagccgaggCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatcat                >  1:3065062/11‑100 (MQ=255)

                                                                                cgccAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatcat                >  2:3688920/1‑100 (MQ=255)

                                                                                 gccAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatcatc               >  2:3205908/1‑100 (MQ=255)

                                                                                  ccAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTAtcatca              >  2:3798183/1‑100 (MQ=255)

                                                                                  ccAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTAtcatca              <  1:1955158/100‑1 (MQ=255)

                                                                                  ccAGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTAtcatca              >  2:1271581/1‑100 (MQ=255)

                                                                                    aGCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatcaagatc            >  1:3231933/1‑95 (MQ=255)

                                                                                     gCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAAcg           >  2:2525241/1‑100 (MQ=255)

                                                                                     gCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAAcg           >  1:2030592/1‑100 (MQ=255)

                                                                                     gCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAAcg           <  2:2457222/100‑1 (MQ=255)

                                                                                     gCCTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAAcg           >  1:3230206/1‑100 (MQ=255)

                                                                                       cTCGGCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAAcgcg         >  1:862023/1‑100 (MQ=255)

                                                                                          ggCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGcac      >  1:1961242/1‑100 (MQ=255)

                                                                                          ggCTGCTAGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGcac      <  2:2284341/100‑1 (MQ=255)

                                                                                            ctgctaGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCacac    >  1:1599373/1‑100 (MQ=255)

                                                                                              gctaGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACCa  >  2:1770717/1‑100 (MQ=255)

AGTTTACTGGTATTGGTCGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACCA  >  NC_000913/3247926‑3248119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`