Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,712,518 (C)6→5 100% intergenic (+360/‑251) nth → / → dtpA DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)/dipeptide and tripeptide permease A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,712,5130C.95.3% 277.8 / 7.1 64intergenic (+355/‑256)nth/dtpADNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)/dipeptide and tripeptide permease A
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base . (30/31);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01

TATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCT  >  NC_000913/1712414‑1712606
                                                                                                   |                                                                                              
tatTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAc                                                                                                >  1:1718538/1‑100 (MQ=255)
      ccTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAccccctc                                                                                          <  1:162491/100‑3 (MQ=255)
       ctaacacaCAATACACTGTCTATTTTTCATTAAATGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATTTTCGGTAAGCCATAAcaccctt                                                                                          <  2:802380/92‑3 (MQ=255)
        tACAAAACATTACACTGGCTATTTTTTTTTTTTTTTACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAccccctctc                                                                                        >  2:1793480/1‑96 (MQ=255)
        tACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATGTCGCCGTTAAGCCATCAccccctctc                                                                                        <  1:1458161/100‑5 (MQ=255)
        tACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAccccctctc                                                                                        >  1:1962430/1‑96 (MQ=255)
        tACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCAccccctctc                                                                                        <  1:1772452/100‑5 (MQ=255)
         aCAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTca                                                                                      >  1:1355826/1‑100 (MQ=255)
         aCAAAACATTACACTGGCTATTTATCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTca                                                                                      >  2:195638/1‑100 (MQ=255)
          cAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcag                                                                                     <  1:103449/100‑1 (MQ=255)
           aaaaCATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagt                                                                                    >  2:1989375/1‑100 (MQ=255)
            aaaCATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagtg                                                                                   >  2:1733493/1‑100 (MQ=255)
            aaaCATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagtg                                                                                   <  1:1844964/100‑1 (MQ=255)
            aaaCATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagt                                                                                    >  2:201586/1‑99 (MQ=255)
            aaaCATTACACTGGCTATTTATCAAAAACTGGACTAACTCACTAGTAAAAACGCTAAATACCAGCACATATCACCGATAAGCCATCACCCCCTCTca                                                                                      >  2:768186/1‑97 (MQ=255)
              aCATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagtgca                                                                                 <  1:2337865/100‑1 (MQ=255)
               cATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTcagtgcag                                                                                <  1:2103435/100‑1 (MQ=255)
                aTTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTcagtgcag                                                                                <  1:1858552/100‑1 (MQ=255)
                  tACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCTCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGa                                                                             <  2:1559299/100‑1 (MQ=255)
                    cacTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGaaa                                                                           >  1:1930973/1‑100 (MQ=255)
                    cacTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGaaa                                                                           >  1:9237/1‑100 (MQ=255)
                      cTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACGTAACGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGaaaaa                                                                         >  2:1684702/1‑100 (MQ=255)
                       tGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGaaaaaa                                                                        <  1:1959831/100‑1 (MQ=255)
                        ngCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAAt                                                                       >  2:2317246/2‑100 (MQ=255)
                        ggCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAAt                                                                       >  2:1969901/1‑100 (MQ=255)
                         gCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATc                                                                      <  1:1939501/100‑1 (MQ=255)
                          cTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCt                                                                     >  1:37650/1‑100 (MQ=255)
                           tATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTg                                                                    <  1:782558/100‑1 (MQ=255)
                              ttttCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGcc                                                                  >  2:582084/1‑99 (MQ=255)
                              ttttCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCg                                                                 >  2:1335214/1‑100 (MQ=255)
                                  cAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTAc                                                             >  1:70743/1‑100 (MQ=255)
                                   aGAAACTGGATTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCATCCCCTCTCTGTGCAGTGAAAAAATCTGCCGTTACg                                                            <  2:1028608/100‑1 (MQ=255)
                                      aaCTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGttt                                                        >  2:825656/1‑100 (MQ=255)
                                      aaCTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACACCCTCTCAGTACAGTGAAAAAATCTGCCGTTACGttt                                                        >  2:948327/1‑100 (MQ=255)
                                         tGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGtttttt                                                     <  1:756991/100‑1 (MQ=255)
                                         tGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGtttttt                                                     <  1:167163/100‑1 (MQ=255)
                                         tGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGtttttt                                                     >  1:2388947/1‑100 (MQ=255)
                                         tGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGtttttt                                                     <  2:1116774/100‑1 (MQ=255)
                                            aCTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTg                                                    <  1:1277184/98‑1 (MQ=255)
                                            aCTATCTCACTAGAAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTg                                                    <  2:1341684/98‑1 (MQ=255)
                                              tATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGaaa                                                 >  2:550263/1‑100 (MQ=255)
                                                    aCTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTaa                                          <  2:242174/100‑1 (MQ=255)
                                                     cTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTa                                           <  1:1373644/98‑1 (MQ=255)
                                                     cTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAAc                                         >  2:5229/1‑100 (MQ=255)
                                                          aaaaaCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTTAAAAAATTTAACGTTg                                     >  2:447635/1‑100 (MQ=255)
                                                           aaaaCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGa                                   >  2:1031806/1‑100 (MQ=255)
                                                            aaaCGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCtag                                    >  2:907908/1‑97 (MQ=255)
                                                               cGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGTAAATTTTAACGCTGGATAAc                               >  2:2167346/1‑100 (MQ=255)
                                                               cGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAAc                               <  2:1410412/100‑1 (MQ=255)
                                                               cGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAAc                               <  1:5229/100‑1 (MQ=255)
                                                               cGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAAc                               <  2:217516/100‑1 (MQ=255)
                                                                gCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACa                              <  2:2190379/100‑1 (MQ=255)
                                                                 cTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACAt                             >  1:2144599/1‑100 (MQ=255)
                                                                  tAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACAt                             <  1:976932/99‑1 (MQ=255)
                                                                      tAGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTccc                        >  2:1655275/1‑100 (MQ=255)
                                                                       aGCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCg                       <  2:1345175/100‑1 (MQ=255)
                                                                        gCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCgg                      <  1:1684722/100‑1 (MQ=255)
                                                                        gCAGAACATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCgg                      <  1:730657/100‑1 (MQ=255)
                                                                           gAACATATCGACGTTAATCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAAt                   <  2:2020748/100‑1 (MQ=255)
                                                                              cATATCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGt                <  1:1048322/100‑1 (MQ=255)
                                                                                 atCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGa             <  2:1967774/100‑1 (MQ=255)
                                                                                  tCGCCGTTAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGaa            >  1:2140382/1‑100 (MQ=255)
                                                                                    gCCGTTAAGCCATCACCACCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAAAGCTGGATAACATTTCCCGGAATGGTTGAAtt          >  2:1520255/1‑100 (MQ=255)
                                                                                        ttAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTcccc      >  1:1550745/1‑100 (MQ=255)
                                                                                        ttAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTcccc      <  1:1031817/100‑1 (MQ=255)
                                                                                        ttAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCAGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTTCCGGAATGGTTGAATTccc       <  2:795638/99‑1 (MQ=255)
                                                                                         tAAGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCg     <  1:825665/100‑1 (MQ=255)
                                                                                          aaGCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGc    >  1:246236/1‑100 (MQ=255)
                                                                                            gCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTTTTTTTTTTTTCTCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCt  >  2:1773610/1‑100 (MQ=255)
                                                                                            gCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCTCTGAATGGTTGAATTCCCCGcc   <  2:930726/99‑1 (MQ=255)
                                                                                            gCCATCACCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCACGCCt  >  1:118134/1‑100 (MQ=255)
                                                                                                   |                                                                                              
TATTGACCTACAAAACATTACACTGGCTATTTTTCAGAAACTGGACTATCTCACTAGTAAAAACGCTAAATAGCAGAACATATCGCCGTTAAGCCATCACCCCCCTCTCAGTGCAGTGAAAAAATCTGCCGTTACGTTTTTTGAAAAATTTAACGCTGGATAACATTTCCCGGAATGGTTGAATTCCCCGCCT  >  NC_000913/1712414‑1712606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: