Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,987,442 Δ1 bp 15.1% intergenic (+14/+65) ftnB → / ← yecJ ferritin B, putative ferrous iron reservoir/DUF2766 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,987,4420T.15.1% 204.9 / 33.5 73intergenic (+14/+65)ftnB/yecJferritin B, putative ferrous iron reservoir/DUF2766 family protein
Reads supporting (aligned to +/- strand):  ref base T (34/28);  new base . (5/6);  total (39/34)
Fisher's exact test for biased strand distribution p-value = 7.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.00e-01

AAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTT  >  NC_000913/1987343‑1987541
                                                                                                   |                                                                                                    
aaGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCt                                                                                                      <  1:2131377/100‑1 (MQ=255)
 aGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTa                                                                                                     >  1:926520/1‑100 (MQ=255)
  gTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTaa                                                                                                    >  1:1094646/1‑100 (MQ=255)
  gTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTaa                                                                                                    <  1:286903/100‑1 (MQ=255)
     ctcAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGc                                                                                                 >  1:1613023/3‑100 (MQ=255)
         gTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCAtt                                                                                              >  1:1783470/1‑99 (MQ=255)
         gTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCAATGCAttag                                                                                            <  1:1818316/100‑3 (MQ=255)
              aaaCTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCg                                                                                       <  2:360797/100‑1 (MQ=255)
              aaaCTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTAACACCAGCTGCATTGATCATCATATGCGCTAATGCATTtcgc                                                                                         >  2:229744/1‑98 (MQ=255)
               aaCTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCAATGCATTAGCGCCGa                                                                                      <  2:1016284/100‑1 (MQ=39)
               aaCTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCGGCATTGATCATCATCGGCGCAATGCATTAGCGCCGa                                                                                      <  2:1700142/100‑1 (MQ=39)
                aCTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGAt                                                                                     <  1:733418/100‑1 (MQ=255)
                aCTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCAATGCATTAGCGCCGAt                                                                                     <  1:1555669/100‑1 (MQ=39)
                aCTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCAATGATCATCATCGGCGCAATGCATTAGCGCCGAt                                                                                     <  2:2262770/100‑1 (MQ=38)
                 cTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATg                                                                                    >  2:1961514/1‑100 (MQ=255)
                 cTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATg                                                                                    >  2:292065/1‑100 (MQ=255)
                     cGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAgg                                                                                >  1:386730/1‑100 (MQ=255)
                     cGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAgg                                                                                >  2:1318362/1‑100 (MQ=255)
                     cGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAgg                                                                                <  1:813652/100‑1 (MQ=255)
                           tgtgCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTga                                                                          <  1:1421966/100‑1 (MQ=255)
                           tgtgCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGATTTga                                                                          >  1:900650/1‑100 (MQ=255)
                           tgtgCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGATTTga                                                                          >  1:61116/1‑100 (MQ=255)
                           tgtgCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTACGCCGATGAAAGTTTTga                                                                          >  1:809454/1‑100 (MQ=255)
                            gtgCTCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTgag                                                                         <  1:1016784/100‑1 (MQ=255)
                            gtgCCCTGTGCAGACCGACCAACATGTTCTGAATGTTGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTgag                                                                         <  1:1688036/100‑1 (MQ=255)
                            gtgCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTgag                                                                         <  1:2169547/100‑1 (MQ=255)
                              gCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGaa                                                                       >  1:312971/1‑100 (MQ=255)
                              gCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGaa                                                                       >  1:1098460/1‑100 (MQ=255)
                                ccTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAAc                                                                     <  1:1696754/100‑1 (MQ=255)
                                   gtgCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATAATCGGCGCTAATGCATTGCACCGATGAAGGTATTGAGAAACCg                                                                   >  1:904566/1‑99 (MQ=255)
                                        gaccgaccAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCt                                                             <  2:1947851/100‑1 (MQ=255)
                                          ccgaccAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCa                                                           >  1:344572/1‑100 (MQ=255)
                                            gaccAACATGGTCTGTATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGCGCAGCCGCTGCctcctc                                                         >  2:1639910/1‑100 (MQ=255)
                                              ccAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATc                                                         >  2:1346712/1‑98 (MQ=255)
                                                aaCATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCAGCATCGGCGCTAATGCATTGAGCCGATGAAGGTTTTGAGAAACCGCTGCCGCATCTGt                                                      >  2:1303662/1‑99 (MQ=255)
                                                  cATGTTCTGAATGTCGTGTCACACCCGCTGCATTGCTCATCATCGGCGCTCATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTg                                                   <  2:705563/100‑1 (MQ=255)
                                                  cATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTg                                                   <  2:31905/100‑1 (MQ=255)
                                                  cATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTg                                                   >  2:379651/1‑100 (MQ=255)
                                                    tGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCTTCaaacagatgag                                                 <  2:386727/100‑11 (MQ=255)
                                                    tGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGaa                                                 >  1:84135/1‑100 (MQ=255)
                                                         tGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAagcagc                                            <  1:110615/100‑1 (MQ=255)
                                                            aTGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGt                                         >  1:1384379/1‑100 (MQ=255)
                                                              gTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGttt                                       >  1:2136055/1‑100 (MQ=255)
                                                               tCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGtttt                                      <  1:306756/100‑1 (MQ=255)
                                                                 gtgtCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGtttttt                                    <  2:867889/100‑1 (MQ=255)
                                                                  tgtCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGttttttt                                   <  1:993519/100‑1 (MQ=255)
                                                                     cacaCCAGGTGCATTGATAATCATCGGAGCTAATGCATTGCGTCGATGAAGGTATTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTGTTTTAAt                                <  2:34622/100‑1 (MQ=255)
                                                                     cacaCCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAAt                                >  1:2398213/1‑100 (MQ=255)
                                                                     cacaCCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAAt                                >  1:2398061/1‑100 (MQ=255)
                                                                      acacCAGCTGCATTGATCATCATCGGCGCTAATGCGTTGCGTCGATGAAGGTTTTGTGAATTCGTTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAAt                                <  2:499339/99‑1 (MQ=255)
                                                                        acCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATggg                             <  2:413678/100‑1 (MQ=255)
                                                                        acCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATgg                              <  1:610770/99‑1 (MQ=255)
                                                                         ccAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGTGa                            >  1:1945966/1‑100 (MQ=255)
                                                                         ccAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGa                            >  1:1685702/1‑100 (MQ=255)
                                                                          cAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGAt                           >  1:1391980/1‑100 (MQ=255)
                                                                           aGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGAtt                          <  1:220691/100‑1 (MQ=255)
                                                                            gCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTc                         >  2:2316062/1‑100 (MQ=255)
                                                                               gCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCAcc                      >  2:553649/1‑100 (MQ=255)
                                                                               ctcaaaaccttcatcGGCGCAATGCATTAGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCAcc                      >  1:1724361/11‑100 (MQ=25)
                                                                                cATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCAccc                     <  2:210714/100‑1 (MQ=255)
                                                                                    gATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCtgtg                 >  1:1251392/1‑100 (MQ=255)
                                                                                        atcatcGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTTTTTGAAGCAGCGGTTTTTTTAATGGGATACACCCTGTgggct             <  1:1622513/100‑3 (MQ=255)
                                                                                        atcatcGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGt             <  2:1603446/100‑1 (MQ=255)
                                                                                          catcGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTaa           <  2:639244/100‑1 (MQ=255)
                                                                                          catcGGCGCAATGCATTAGCGCCGCTGAAGGTTCTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTa            >  1:2322685/1‑99 (MQ=38)
                                                                                           atcGGCGCAATGCATTAGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTaaa          >  1:2260168/1‑100 (MQ=39)
                                                                                           atcGGCGCAATGCATTAGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTaaa          >  1:790350/1‑100 (MQ=39)
                                                                                           atcGGCGCAATGCATTAGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTaaa          >  2:493136/1‑100 (MQ=39)
                                                                                            tcGGCGCAATGCATTAGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAAc         <  2:1724340/100‑1 (MQ=39)
                                                                                              ggCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGAGTAAAc         >  1:18527/1‑98 (MQ=255)
                                                                                                cgcTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGa     >  1:2038869/1‑100 (MQ=255)
                                                                                                 gcTAATTCATTGTGGCAATGAAGATTTTGAGAAACCGCTGCCTCATCTGTTAGAAGAAGCGTGTTATTTAATGGGTGTCAGCCGCTGGGGTAAAc         <  2:1597504/95‑1 (MQ=255)
                                                                                                 gcTAATGCATTGCGCCGATGAAGGTTTTGTGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAg    <  2:1444455/100‑1 (MQ=255)
                                                                                                  cTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAg    >  1:412531/1‑99 (MQ=255)
                                                                                                   tAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTTATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGt   <  2:409071/99‑1 (MQ=255)
                                                                                                   tAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGtt  >  1:1484912/1‑100 (MQ=255)
                                                                                                   tAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGtt  <  1:118671/100‑1 (MQ=255)
                                                                                                   |                                                                                                    
AAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTT  >  NC_000913/1987343‑1987541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: