| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,609,561:1 | +C | 19.5% | coding (1120/1452 nt) | uxaB ← | altronate oxidoreductase, NAD‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,609,561 | 1 | . | C | 19.5% | 112.9 / 32.9 | 41 | coding (1120/1452 nt) | uxaB | altronate oxidoreductase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base . (18/15); new base C (5/3); total (23/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.83e-01 | |||||||||||
GCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCAC > NC_000913/1609463‑1609659 | gCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAACGTAGGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTCCCCTCCCAGCCGCtg‑t > 2:779499/1‑100 (MQ=255) ccGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCtg‑tg < 2:28403/100‑1 (MQ=255) cGTTACGCTCACCATGATAGAAAGCAATCAATGAAGCTAATGCGAAACTCAGACGAGCCGGAAGTGAGCCGTTTGCATTCCGCTCTGCCCGCAGCtg‑tcg > 2:866541/1‑98 (MQ=255) cGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑Tgg < 1:1502432/100‑1 (MQ=255) cGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGa > 2:1506786/1‑100 (MQ=255) cGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCA‑CAGCTGCTGGCAggg < 1:1056748/100‑2 (MQ=255) cGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCA‑CAGCTGCTGGCAggg < 1:597941/100‑2 (MQ=255) cGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCA‑CAGCTGCTGGCAggg < 1:514161/100‑2 (MQ=255) ccacGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCggg < 1:1848369/100‑1 (MQ=255) ccacGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCggg > 1:1003450/1‑100 (MQ=255) cGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGc > 2:1737855/1‑100 (MQ=255) gATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCg < 2:2275616/100‑1 (MQ=255) tAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCgg < 1:984363/99‑1 (MQ=255) gAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAAc > 2:147920/1‑100 (MQ=255) cGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTg > 1:400037/1‑100 (MQ=255) gCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTgg < 2:1521484/100‑1 (MQ=255) caatcaatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGt > 1:244683/1‑100 (MQ=255) atcaatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCa < 1:304308/100‑1 (MQ=255) aTGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAgcgc < 1:1120320/100‑1 (MQ=255) gCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGa > 1:1902779/1‑100 (MQ=255) gCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGa < 2:1003439/100‑1 (MQ=255) cGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGcgcg > 2:2051335/1‑98 (MQ=255) aaaGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAg > 1:2387506/1‑100 (MQ=255) aaaGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAg > 2:2130983/1‑100 (MQ=255) aGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAc > 1:996868/1‑100 (MQ=255) gTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAca < 2:2015791/100‑1 (MQ=255) gcgcCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTg < 2:2178079/100‑1 (MQ=255) cgcCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGa > 1:1088199/1‑100 (MQ=255) gcCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGTATGCGGGTGCGGAACTTGGTCATACCGTGGAGCGCGATAGACAGCAGCTGAt < 2:1094441/100‑1 (MQ=255) ggAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGATCGCGATAGACAGCAGCTGATGCt < 2:1350103/100‑1 (MQ=255) ggAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCt < 1:454800/100‑1 (MQ=255) aGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTaa > 1:1745534/1‑100 (MQ=255) ttGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGtt > 2:429640/1‑100 (MQ=255) ttCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGa < 1:1813522/100‑1 (MQ=255) gcatcctgccA‑CAGCTGCTGGCAGGATGCGGGTGCGGAACTTGTTCATACCGTTGAGCGCGATAGACAGAAGCTGATGCTTACTGTACGGGTTACGGaaa > 2:2284855/5‑100 (MQ=255) ctgccctgccAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACAGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGaaa > 2:145690/1‑100 (MQ=255) catcctgccA‑CAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAAc > 1:560002/4‑100 (MQ=255) catcctgccA‑CAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAAc > 1:1760799/4‑100 (MQ=255) gccctgccAGCAGCTG‑TGGCAGGATGCTGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACg > 2:175001/1‑100 (MQ=255) gccctgccAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACg > 2:1573720/1‑100 (MQ=255) atcctgccA‑CAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGATACGGaaa > 1:437576/3‑98 (MQ=255) cctgccA‑CAGCTGCTGGCAGGATGCGGGTGAGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTGCGGAAACGcc > 2:1574729/1‑100 (MQ=255) ccAGCAGCTG‑TGGCAGGATGCGGGTCCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATTTATGGGTTACGGAAACGCCCGGt < 2:157918/100‑1 (MQ=255) gcagcTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGCGAc > 2:443565/1‑100 (MQ=255) tg‑tgGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCAc < 1:1552548/100‑1 (MQ=255) | GCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTG‑TGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCAC > NC_000913/1609463‑1609659 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |