BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	11,755	A→T	22.5%	L11Q (CTG→CAG)	yaaI ←	UPF0412 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	11,755	0	A	T	22.5%	221.9 / 64.4	112	L11Q (CTG→CAG)	yaaI	UPF0412 family protein
Reads supporting (aligned to +/- strand): ref base A (38/48); new base T (17/8); total (55/57)
Fisher's exact test for biased strand distribution p-value = 4.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 112 total aligned reads displayed.

GCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAG  >  NC_000913/11657‑11853

gctccAGATCGGTGGTTTCGTTACGTGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTACCTGCGCCGTGTAGCTCAGCATCAGGCTCATCGCCAg                                                                                                   <  2:234523/97‑1 (MQ=255)

gcgcCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAg                                                                                                   <  1:1817366/100‑1 (MQ=255)

 cgcCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAgg                                                                                                  >  2:1963981/1‑100 (MQ=255)

  nccAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGc                                                                                                 >  1:878215/2‑100 (MQ=255)

  gcCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCTGGc                                                                                                 >  2:1720545/1‑100 (MQ=255)

  gcCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAgg                                                                                                  >  1:1981526/1‑99 (MQ=255)

  gcCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGc                                                                                                 >  1:1212304/1‑100 (MQ=255)

     aGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCTGGCTg                                                                                               >  1:320196/1‑99 (MQ=255)

      gATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGc                                                                                             <  2:531175/100‑1 (MQ=255)

      gATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGc                                                                                             >  1:1598277/1‑100 (MQ=255)

         cGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCTGGCTGGCGGa                                                                                          >  1:2326135/1‑100 (MQ=255)

         cGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCgg                                                                                           <  1:1654354/99‑1 (MQ=255)

          gTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGACGGaa                                                                                         >  2:1524373/1‑100 (MQ=255)

              gTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCTGGCTGGCGGAAATCg                                                                                     >  1:668717/1‑100 (MQ=255)

              gTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCg                                                                                     <  1:815712/100‑1 (MQ=255)

               tttCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGt                                                                                    <  2:1296817/100‑1 (MQ=255)

               tttCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAAGCGt                                                                                    <  2:245590/100‑1 (MQ=255)

                ttCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCTGGCTGGCGGAAATCGTa                                                                                   >  2:2123150/1‑100 (MQ=255)

                ttCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTa                                                                                   <  1:2385290/100‑1 (MQ=255)

                 tCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTaa                                                                                  >  1:1340471/1‑100 (MQ=255)

                      aCGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACg                                                                             <  1:2063841/100‑1 (MQ=255)

                       cgcgGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACgg                                                                            >  2:765757/1‑100 (MQ=255)

                        gcgGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGa                                                                           >  1:2209130/1‑100 (MQ=255)

                           gCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGAttt                                                                        >  1:405840/1‑100 (MQ=255)

                             aTTGCAATGGCGCCGAGGAGTTTATGTTCGTTTCCCTGCGCCTTGCAGCATAGCCTCAGGCTAAGCGCCATGCTGGCAGAAATCGTAAAAACTGAtttta                                                                      <  2:2259929/100‑3 (MQ=255)

                             aTTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCa                                                                      >  2:736866/1‑100 (MQ=255)

                              ttGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCAt                                                                     <  1:630497/100‑1 (MQ=255)

                                gCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCTGGCTGGCGGAAATCGTAAAAACGGATTTCATaa                                                                   >  2:564347/1‑100 (MQ=255)

                                gCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATaa                                                                   >  1:1962327/1‑100 (MQ=255)

                                 cAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCTGGCTGGCGGAAATCGTAAAAACGGATTTCATAAg                                                                  >  2:1484346/1‑100 (MQ=255)

                                 cAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAg                                                                  >  1:905216/1‑100 (MQ=255)

                                  aaTGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCTGGCTGGCGGAAATCGTAAAAACGGATTTCATAAgg                                                                 >  1:428055/1‑100 (MQ=255)

                                  aaTGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAgg                                                                 <  2:1347243/100‑1 (MQ=255)

                                      gcgcCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATtc                                                             <  1:968334/100‑1 (MQ=255)

                                          cGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTctct                                                          <  1:569370/99‑1 (MQ=255)

                                          cGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCtt                                                         <  1:2046199/100‑1 (MQ=255)

                                          cGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCtt                                                         >  1:947257/1‑100 (MQ=255)

                                           gaggagTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTa                                                        <  2:797844/100‑1 (MQ=255)

                                           gaggagTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGAAAAAAAAAAAAAAACGGATTTCATAAGGATTCTCTTa                                                        <  2:796459/100‑1 (MQ=255)

                                             ggagTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGt                                                      <  2:2164257/100‑1 (MQ=255)

                                             ggagTATACGGTCGTTTGCCTGCGCCGTGCTACAGGCTCTCAGGCTTATCGTCCAGCAGGCGGACCTCGTAATATCGGTTTTCATAAGTAGTctct                                                          <  2:1394786/96‑1 (MQ=255)

                                              gagTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTg                                                     <  2:1887227/100‑1 (MQ=255)

                                               agTTTATGGTCGTTTGCCTGCGCCGTGCATCACAGCATCAGGCTAATCGCCAGGCTGGAAAAAAAAAAAAAAACGGATTTCATAAGGATTCTCTTAGTg                                                     <  2:1769517/99‑1 (MQ=255)

                                               agTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTgg                                                    <  1:952254/100‑1 (MQ=255)

                                                gTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTggg                                                   >  1:2374001/1‑100 (MQ=255)

                                                 tttATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTgg                                                    <  1:1132881/98‑1 (MQ=255)

                                                  ttATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGaa                                                 <  1:610961/100‑1 (MQ=255)

                                                    aTGGTCGTTTGCCTGCGTCGTGCAGCAAAATATCAGGTTAATCGAGTGGATGAAAAAAAAAATAAAAACGGATTTCATAAGGATTCTCTTAGTGGGa                                                  <  2:67013/97‑1 (MQ=255)

                                                    aTGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAaga                                               <  1:1576975/100‑1 (MQ=255)

                                                    aTGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGAGTCTCTTAGTGGGAaga                                               >  2:1401171/1‑100 (MQ=255)

                                                     tGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCTGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAagag                                              <  1:68862/100‑1 (MQ=255)

                                                     tGGTCGTTTGCCTGCGCCGTGCAACACAGCATCAGGCTAATCGCCTGGCTGGCAGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAaga                                               <  2:442348/99‑1 (MQ=255)

                                                      ggTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAgg                                             >  1:1128446/1‑100 (MQ=255)

                                                          gTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAgg                                         >  1:1648511/1‑100 (MQ=255)

                                                           tttGCTTGCGCCGTGTAGCACAGCATCAGGTTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAggg                                        <  1:615257/100‑1 (MQ=255)

                                                              gCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCTGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAggggg                                      >  2:738061/1‑99 (MQ=255)

                                                              gCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCTGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGa                                     >  1:1969599/1‑100 (MQ=255)

                                                              gCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGa                                     >  1:1933614/1‑100 (MQ=255)

                                                                cTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATg                                   <  2:2052569/100‑1 (MQ=255)

                                                                cTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATg                                   <  2:2374417/100‑1 (MQ=255)

                                                                 tGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATg                                   <  2:2403426/99‑1 (MQ=255)

                                                                 tGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATg                                   <  2:1952907/99‑1 (MQ=255)

                                                                    gcCGTGCAGCACAGCATCAGGCTAATCGCCTGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAAt                                >  2:1423150/1‑99 (MQ=255)

                                                                    gcCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATa                               >  1:2144300/1‑100 (MQ=255)

                                                                       gTGCAGCACAGCATCAGGCTAATTGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTTTCTTAGTGGGAAGAGGTAGGGGGATGAATAcc                             <  2:212339/99‑1 (MQ=255)

                                                                       gTGCAGCACAGCATCAGGCTAATCGCCTGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATAccc                            >  1:1031166/1‑100 (MQ=255)

                                                                       gTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATAccc                            <  2:2240029/100‑1 (MQ=255)

                                                                       gTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATAccc                            <  2:2373973/100‑1 (MQ=255)

                                                                          cagcacagcaTCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACt                         >  2:2120417/1‑100 (MQ=255)

                                                                           agcacagcaTCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACt                         <  2:110212/99‑1 (MQ=255)

                                                                            gcacagcaTCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAg                       >  1:2310253/1‑100 (MQ=255)

                                                                                 gcaTCAGGCTAATCGCCTGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTAc                  >  2:613237/1‑100 (MQ=255)

                                                                                 gcaTCAGGCTAATCGCCTGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTAc                  <  2:743098/100‑1 (MQ=255)

                                                                                 gcaTCAGGCTAATCGCCAGGGTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTAc                  >  2:251602/1‑100 (MQ=255)

                                                                                 gcaTCAGGCTAATCGCCAGGCTGGCGGAACTCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTAc                  <  2:1786039/100‑1 (MQ=255)

                                                                                 gcaTCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTAc                  <  1:1721367/100‑1 (MQ=255)

                                                                                  caTCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTAct                 >  2:1412934/1‑100 (MQ=255)

                                                                                   aTCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTActg                >  2:2099029/1‑100 (MQ=255)

                                                                                    tCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTActgc               >  2:1248100/1‑100 (MQ=255)

                                                                                     cAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTActgct              >  2:920724/1‑100 (MQ=255)

                                                                                     cAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTActgct              <  2:1962305/100‑1 (MQ=255)

                                                                                     cAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTActgct              >  1:2315751/1‑100 (MQ=255)

                                                                                       ggCTAATCGCCTGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGa            >  1:1311710/1‑100 (MQ=255)

                                                                                       ggCTAATCGCCTGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGa            <  1:1589732/100‑1 (MQ=255)

                                                                                       ggCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGa            >  2:1649823/1‑100 (MQ=255)

                                                                                       ggCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGa            >  2:1375584/1‑100 (MQ=255)

                                                                                       ggCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGa            >  2:625757/1‑100 (MQ=255)

                                                                                         cTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGCAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATa          >  1:694289/1‑100 (MQ=255)

                                                                                         cTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGAt           <  1:1063331/99‑1 (MQ=255)

                                                                                         cTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTAACTGCTGATa          >  1:347201/1‑100 (MQ=255)

                                                                                          tAATCGCCTGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATaa         >  2:2281287/1‑100 (MQ=255)

                                                                                           aaTCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATaaa        >  2:1378312/1‑100 (MQ=255)

                                                                                            aTCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAag       >  2:1843679/1‑100 (MQ=255)

                                                                                            aTCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTATCTTAGTGGGAAGAGGTAGGGGCAAGAATACCCACTAGTTTACTGCTGATAAag       >  2:586683/1‑100 (MQ=255)

                                                                                            aTCGCCAGGCTGGCGGAAATCGTAAAAACGGATGTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAag       <  2:1160083/100‑1 (MQ=255)

                                                                                             tCGCCTGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAaga      <  2:2326107/100‑1 (MQ=255)

                                                                                              cGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAagag     <  1:1401186/100‑1 (MQ=255)

                                                                                                ccAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAAGagaa   <  1:2117680/100‑1 (MQ=255)

                                                                                                 cAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAAGagaag  <  1:736874/100‑1 (MQ=255)

                                                                                                 cAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAAGagaag  <  1:130214/100‑1 (MQ=255)

GCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAG  >  NC_000913/11657‑11853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`