| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,452,339 | A→G | 38.9% | intergenic (‑3/+17) | yfcS ← / ← yfcU | putative periplasmic pilin chaperone/pseudogene, FimD fimbrial export usher family;putative membrane; Not classified; putative outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,452,339 | 0 | A | G | 38.9% | 46.7 / 91.3 | 85 | intergenic (‑3/+17) | yfcS/yfcU | putative periplasmic pilin chaperone/pseudogene, FimD fimbrial export usher family;putative membrane; Not classified; putative outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (25/27); new base G (16/17); total (41/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01 | |||||||||||
TGGCGTTACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGACAGGCTTTATG > NC_000913/2452241‑2452434 | tGGCGTTACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGa < 2:2163506/99‑1 (MQ=255)tGGCGTTACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGAt > 1:2284176/1‑100 (MQ=255) cGTTACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCAt > 1:1968676/1‑100 (MQ=255) tgtaaCGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCt < 2:1188027/96‑1 (MQ=255) aCAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCt < 2:925354/100‑1 (MQ=255) cAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCtt < 2:1938389/100‑1 (MQ=255) cAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCt > 2:1921811/1‑99 (MQ=255) cAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCtt < 2:1595822/100‑1 (MQ=255) cAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCtt < 2:1757223/100‑1 (MQ=255) gACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTaa < 1:312791/100‑1 (MQ=255) aCGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAAt < 2:1190104/100‑1 (MQ=255) gCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCa < 1:1306517/100‑1 (MQ=255) aGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACg > 1:541398/1‑99 (MQ=255) gggCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGtt > 1:319501/1‑100 (MQ=255) ggCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTa > 1:2189316/1‑100 (MQ=255) ggCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTa > 1:1040606/1‑100 (MQ=255) ggCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTa < 1:1854135/100‑1 (MQ=255) ggCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTa > 1:1090226/1‑100 (MQ=255) gCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTAt > 1:1363408/1‑100 (MQ=255) gACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTgg > 2:1216128/1‑100 (MQ=255) aCAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGt < 1:1245284/100‑1 (MQ=255) aCAAACTGGTAGCACTAACCAGTAACGCCAGCGTCATAGCACCCAATTTAGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGt > 2:1477951/1‑100 (MQ=255) cAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGtt > 2:1415594/1‑100 (MQ=255) aaaCTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTa < 2:171232/100‑1 (MQ=255) aaaCTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAAGCAGACATGGGTCATTCCTTAATCACGTTATTGGTTa > 1:1682631/1‑100 (MQ=255) aCTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGc > 1:2185691/1‑100 (MQ=255) cTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCt > 1:1398298/1‑100 (MQ=255) cTGGTAGCGCTAAGCAGTAACGCAAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGc < 2:511793/99‑1 (MQ=255) ggTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGCTGTTGGTTAGCTga > 1:1534430/1‑100 (MQ=255) gTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTgaa < 1:1921833/100‑1 (MQ=255) tAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTgaaa > 1:983851/1‑100 (MQ=255) aGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTgaaac > 1:1490547/1‑100 (MQ=255) gcTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTgaaacaga > 1:829454/1‑100 (MQ=255) tAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCATTAATCACGTTATTGGTTAGATgaaacagaaa > 2:1487209/1‑100 (MQ=255) aaGCAGTAACGCCACCGTCATAGCGCCCATTTTGGAAGAGCAAAGTAAATCTGACATGGATCATTCCTTAATCTCGTTATTGGTTAGCTgaaacagaaac < 2:404575/100‑1 (MQ=255) gTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAgggg < 1:770866/100‑1 (MQ=255) gTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAgggg > 2:2120657/1‑100 (MQ=255) tAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAggggg < 1:1690911/100‑1 (MQ=255) acaccAGCGGCATAGAACCAAATTTGGCAGAGAAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGAt < 1:2150664/97‑1 (MQ=255) aCGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGAt > 2:1956015/1‑100 (MQ=255) cGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATc < 2:311871/100‑1 (MQ=255) cGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATc > 1:1248250/1‑100 (MQ=255) gCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCg > 1:1098790/1‑100 (MQ=255) ccAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCgg > 1:151205/1‑100 (MQ=255) cAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCggt < 2:2114117/100‑1 (MQ=255) aGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCggtg > 2:1295564/1‑100 (MQ=255) cGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCggtggg < 1:326878/100‑1 (MQ=255) cGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCggtggg < 1:2193033/100‑1 (MQ=255) cGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCggtggn < 2:217183/100‑2 (MQ=255) cGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCggtggg < 2:2181669/100‑1 (MQ=255) tCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCggtgggtg < 1:566977/100‑1 (MQ=255) tgggagaaaaTGTGGCATAGCAAATGAAATCGGAAGTGGATCAGTCCTGAATCCCGTTAGTGGTTGGCTGAAACAGAAACAGGGGTAGCggtgggtg < 2:2000493/89‑1 (MQ=255) tAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCa > 1:2315044/1‑100 (MQ=255) aGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCAc < 1:1839793/100‑1 (MQ=255) cccAATTTGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGt > 2:550942/1‑100 (MQ=255) cAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTg < 2:1090216/100‑1 (MQ=255) tttgCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTc < 2:2032893/97‑1 (MQ=255) ttGGCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTc < 1:2012828/100‑1 (MQ=255) ttGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTc < 2:1261422/100‑1 (MQ=255) ttGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTc < 2:1414575/100‑1 (MQ=255) tGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCt < 2:1038896/100‑1 (MQ=255) ggCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTc < 1:550945/98‑1 (MQ=255) ggCAGAGCAAAGTAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTg > 1:263689/1‑100 (MQ=255) ggCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTTACCCGTTTCGTCTg > 1:813818/1‑100 (MQ=255) ggCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTg > 2:1863359/1‑100 (MQ=255) gCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGc < 1:2404199/100‑1 (MQ=255) gCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGc > 2:1646223/1‑100 (MQ=255) gCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGCTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTAACCTGTTGCGTCTGc > 2:527245/1‑100 (MQ=255) agagCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTc > 2:2096194/1‑100 (MQ=255) gagCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTcc < 2:1264567/100‑1 (MQ=255) aaaGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGaa > 1:2081675/1‑100 (MQ=255) aaaGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGaa > 2:2098071/1‑100 (MQ=255) aaaGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGaa < 1:924460/100‑1 (MQ=255) aGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATg > 2:1167126/1‑100 (MQ=255) aGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATg < 2:2178595/100‑1 (MQ=255) aGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATg < 2:1248237/100‑1 (MQ=255) gTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGa < 2:1696137/100‑1 (MQ=255) tAAATCAGACATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGAc < 1:1440391/100‑1 (MQ=255) aaaTCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGACa < 2:2081653/100‑1 (MQ=255) aaTCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGACAg > 1:1245937/1‑100 (MQ=255) gACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGACAGGCttt > 2:546132/1‑100 (MQ=255) aCATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGACAGGCTTTa < 2:2227517/100‑1 (MQ=255) cATGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGACAGGCTTTAt > 2:1540170/1‑100 (MQ=255) cATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGACAGGCTTTAt < 2:358888/100‑1 (MQ=255) aTGGGTCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGACAGGCTTTATg > 1:1044672/1‑100 (MQ=255) aTGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGACAGGCTTTAt < 2:587777/99‑1 (MQ=255) | TGGCGTTACAGACGCCAGGGCAGACAAACTGGTAGCGCTAAGCAGTAACGCCAGCGTCATAGCACCCAATTTGGCAGAGCAAAGTAAATCAGACATGGATCATTCCTTAATCACGTTATTGGTTAGCTGAAACAGAAACAGGGGGATCGGTGGGTGTCACCTGTTGCGTCTGCTCCTGAATGACAGGCTTTATG > NC_000913/2452241‑2452434 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |