Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,521,967 C→A 100% A171S (GCG→TCG)  xapR ← transcriptional activator of xapAB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,521,9670CA97.4% 246.6 / ‑0.8 78A171S (GCG→TCG) xapRtranscriptional activator of xapAB
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (38/37);  total (40/38)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.36e-01

GGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCA  >  NC_000913/2521870‑2522062
                                                                                                 |                                                                                               
ggcggcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAacgacg                                                                                               >  1:67462/1‑100 (MQ=255)
  cggcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGAt                                                                                             >  1:1485846/1‑100 (MQ=255)
   ggcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGAtt                                                                                            <  1:646179/100‑1 (MQ=255)
    gcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTc                                                                                           <  2:2291252/100‑1 (MQ=255)
      aTTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCAt                                                                                         >  1:787205/1‑100 (MQ=255)
      aTTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACAGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCAt                                                                                         <  1:1799048/100‑1 (MQ=255)
       ttGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATg                                                                                        <  1:1668574/100‑1 (MQ=255)
        tGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGc                                                                                       <  2:10198/100‑1 (MQ=255)
         gTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCa                                                                                      >  2:1244095/1‑100 (MQ=255)
         gTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCa                                                                                      >  2:53214/1‑100 (MQ=255)
          tAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCaa                                                                                     >  1:2045601/1‑100 (MQ=255)
           aaCAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAAc                                                                                    >  1:24838/1‑100 (MQ=255)
            aCAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAAcc                                                                                   >  1:551782/1‑100 (MQ=255)
             cAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCg                                                                                  <  2:436340/100‑1 (MQ=255)
               ataTACTCGTCACGTAGCGCTTCCTTCGGGACCGTGGAAAATAATGATAAAAGATGCTCTTCAGGCATCGTCACCAGAAACGACGATTCATGCAACCg                                                                                  <  2:1344701/96‑1 (MQ=255)
                 aTACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAg                                                                              <  1:2359356/100‑1 (MQ=255)
                   aCTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCt                                                                            <  2:2153269/100‑1 (MQ=255)
                    cTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTg                                                                           >  1:1579826/1‑100 (MQ=255)
                       gTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTa                                                                        >  1:947102/1‑100 (MQ=255)
                           cGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAAc                                                                    <  1:212277/100‑1 (MQ=255)
                           cGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAAc                                                                    <  1:2135862/100‑1 (MQ=255)
                               gcgcTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAg                                                                 >  2:2128738/1‑99 (MQ=255)
                                cgcTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTc                                                               >  2:2141873/1‑100 (MQ=255)
                                        gCGGTACGGTGGAAAATGATGAGAGATGATGATCTTGAGGCATCGCAACCAGAAACGACGATTCATACAACCGTAAGCTGGTAAAACCAGTCggtg                                                           >  2:338661/1‑96 (MQ=255)
                                        gCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTc                                                       <  1:1005517/100‑1 (MQ=255)
                                        gCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTc                                                       <  2:201604/100‑1 (MQ=255)
                                        gCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTc                                                       <  1:1007857/100‑1 (MQ=255)
                                           ggACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGt                                                    >  1:1987969/1‑100 (MQ=255)
                                              cGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGc                                                 <  2:420080/100‑1 (MQ=255)
                                               ggTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGcc                                                >  1:2202828/1‑100 (MQ=255)
                                               ggTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATACAACCGTAAGCTGCTAAAACCAGTCGGTGGTTCTGTCGcc                                                >  2:602625/1‑100 (MQ=255)
                                                gTGGAAAATGATGAGAGATGGTGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCa                                               >  2:1464180/1‑100 (MQ=255)
                                                  ggAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCAtt                                             >  1:1440407/1‑100 (MQ=255)
                                                    aaaaTGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGACAtt                                             >  1:627197/1‑98 (MQ=255)
                                                     aaaTGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGc                                          <  1:1821957/100‑1 (MQ=255)
                                                      aaTGATGAGAGATGATGCTCTTCAGGCATCGCTACCAGAAACGACGATTCATGCAACCGTAATCTGGTAAATCCAGTCGGTTTTTCTGTAGCCATTCGcc                                         <  2:1298849/100‑1 (MQ=255)
                                                      aaTGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGcc                                         <  1:2144396/100‑1 (MQ=255)
                                                       atgatgAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTttcgccattcgcca                                        >  1:862301/1‑100 (MQ=255)
                                                       atgatgAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCa                                        <  2:1985661/100‑1 (MQ=255)
                                                        tgatgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAg                                       <  2:930061/100‑1 (MQ=255)
                                                        tgatgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAg                                       >  1:988423/1‑100 (MQ=255)
                                                        tgatgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAg                                       >  2:1731483/1‑100 (MQ=255)
                                                         gatgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGa                                      >  2:1416303/1‑100 (MQ=255)
                                                          atgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCTTTCGCCAGa                                      >  1:907648/1‑99 (MQ=255)
                                                           tgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGAt                                     >  1:966353/1‑99 (MQ=255)
                                                            gagagaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATcc                                   >  1:1161690/1‑100 (MQ=255)
                                                               agaTGATGCTCTTCAGGCATCGCCACAAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCgg                                <  1:686612/100‑1 (MQ=255)
                                                                gatgatGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGc                               <  1:814857/100‑1 (MQ=255)
                                                                gatgatGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGc                               >  2:1190773/1‑100 (MQ=255)
                                                                gatgatGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGc                               <  2:1790052/100‑1 (MQ=255)
                                                                 atgatgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCa                              <  1:107239/100‑1 (MQ=255)
                                                                    atgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCACTCGCCAGATCCCGGCATCa                           <  1:808435/100‑1 (MQ=255)
                                                                      gCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAg                         <  1:1991719/100‑1 (MQ=255)
                                                                      gCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAg                         <  2:360993/100‑1 (MQ=255)
                                                                        tctTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGtt                       >  2:198647/1‑100 (MQ=255)
                                                                         ctTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTc                      >  2:482195/1‑100 (MQ=255)
                                                                         ctTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTc                      >  1:530394/1‑100 (MQ=255)
                                                                           tCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTcgc                    >  1:1914837/1‑100 (MQ=255)
                                                                            cAGGCATCGCCACCAGAAACGATGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTTGTTCTGTTGCCATTCGCCAGATCCCGGCATCAAGTTcgcg                   <  2:178290/100‑1 (MQ=255)
                                                                            cAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTcgcg                   <  2:988412/100‑1 (MQ=255)
                                                                             aGGCATCGCCACCAGATACGACGATTCATGCAATCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcgg                  <  2:2145546/100‑1 (MQ=255)
                                                                             aGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTcgcg                   <  1:1841625/99‑1 (MQ=255)
                                                                             aGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcgg                  >  2:1808749/1‑100 (MQ=255)
                                                                             aGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcgg                  <  1:2141897/100‑1 (MQ=255)
                                                                             aGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcgg                  <  1:727742/100‑1 (MQ=255)
                                                                               gCATCGCCACCAGAAACGTCGATTAATGCAAGCGAAAGCTGCTAAATCAAGTCGGTGGTTCTGTCGCAATTTGCCAGATCAAGGCATCAAGTTGgcgg                  <  2:1086203/98‑1 (MQ=255)
                                                                                cATCGCCACCAGAAATGACGATTGATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGTCATTCGCCAGATCCCGGCATCAAGTTCTCGGCGt               >  2:1551465/1‑100 (MQ=255)
                                                                                   cGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTcc            >  1:763727/1‑100 (MQ=255)
                                                                                   cGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTcc            <  2:551779/100‑1 (MQ=255)
                                                                                    gCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCa           >  1:915829/1‑100 (MQ=255)
                                                                                    gCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGCGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCa           >  2:676574/1‑100 (MQ=255)
                                                                                        ccaGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCaa       >  1:46871/1‑100 (MQ=255)
                                                                                         caGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAg      >  1:1396914/1‑100 (MQ=255)
                                                                                          aGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAgg     >  2:412318/1‑100 (MQ=255)
                                                                                          aGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAgg     <  1:476022/100‑1 (MQ=255)
                                                                                           gAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGc    <  2:218068/100‑1 (MQ=255)
                                                                                           gAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGc    >  1:1015079/1‑100 (MQ=255)
                                                                                             aaCGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCa  <  1:1287837/100‑1 (MQ=255)
                                                                                             aaCGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCa  >  1:1125163/1‑100 (MQ=255)
                                                                                                 |                                                                                               
GGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCA  >  NC_000913/2521870‑2522062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: