Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,521,967 | C→A | 100% | A171S (GCG→TCG) | xapR ← | transcriptional activator of xapAB |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,521,967 | 0 | C | A | 97.4% | 246.6 / ‑0.8 | 78 | A171S (GCG→TCG) | xapR | transcriptional activator of xapAB |
Reads supporting (aligned to +/- strand): ref base C (2/0); new base A (38/37); total (40/38) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.94e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.36e-01 |
GGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCA > NC_000913/2521870‑2522062 | ggcggcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAacgacg > 1:67462/1‑100 (MQ=255) cggcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGAt > 1:1485846/1‑100 (MQ=255) ggcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGAtt < 1:646179/100‑1 (MQ=255) gcATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTc < 2:2291252/100‑1 (MQ=255) aTTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCAt > 1:787205/1‑100 (MQ=255) aTTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACAGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCAt < 1:1799048/100‑1 (MQ=255) ttGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATg < 1:1668574/100‑1 (MQ=255) tGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGc < 2:10198/100‑1 (MQ=255) gTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCa > 2:1244095/1‑100 (MQ=255) gTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCa > 2:53214/1‑100 (MQ=255) tAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCaa > 1:2045601/1‑100 (MQ=255) aaCAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAAc > 1:24838/1‑100 (MQ=255) aCAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAAcc > 1:551782/1‑100 (MQ=255) cAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCg < 2:436340/100‑1 (MQ=255) ataTACTCGTCACGTAGCGCTTCCTTCGGGACCGTGGAAAATAATGATAAAAGATGCTCTTCAGGCATCGTCACCAGAAACGACGATTCATGCAACCg < 2:1344701/96‑1 (MQ=255) aTACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAg < 1:2359356/100‑1 (MQ=255) aCTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCt < 2:2153269/100‑1 (MQ=255) cTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTg > 1:1579826/1‑100 (MQ=255) gTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTa > 1:947102/1‑100 (MQ=255) cGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAAc < 1:212277/100‑1 (MQ=255) cGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAAc < 1:2135862/100‑1 (MQ=255) gcgcTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAg > 2:2128738/1‑99 (MQ=255) cgcTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTc > 2:2141873/1‑100 (MQ=255) gCGGTACGGTGGAAAATGATGAGAGATGATGATCTTGAGGCATCGCAACCAGAAACGACGATTCATACAACCGTAAGCTGGTAAAACCAGTCggtg > 2:338661/1‑96 (MQ=255) gCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTc < 1:1005517/100‑1 (MQ=255) gCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTc < 2:201604/100‑1 (MQ=255) gCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTc < 1:1007857/100‑1 (MQ=255) ggACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGt > 1:1987969/1‑100 (MQ=255) cGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGc < 2:420080/100‑1 (MQ=255) ggTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGcc > 1:2202828/1‑100 (MQ=255) ggTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATACAACCGTAAGCTGCTAAAACCAGTCGGTGGTTCTGTCGcc > 2:602625/1‑100 (MQ=255) gTGGAAAATGATGAGAGATGGTGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCa > 2:1464180/1‑100 (MQ=255) ggAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCAtt > 1:1440407/1‑100 (MQ=255) aaaaTGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGACAtt > 1:627197/1‑98 (MQ=255) aaaTGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGc < 1:1821957/100‑1 (MQ=255) aaTGATGAGAGATGATGCTCTTCAGGCATCGCTACCAGAAACGACGATTCATGCAACCGTAATCTGGTAAATCCAGTCGGTTTTTCTGTAGCCATTCGcc < 2:1298849/100‑1 (MQ=255) aaTGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGcc < 1:2144396/100‑1 (MQ=255) atgatgAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTttcgccattcgcca > 1:862301/1‑100 (MQ=255) atgatgAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCa < 2:1985661/100‑1 (MQ=255) tgatgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAg < 2:930061/100‑1 (MQ=255) tgatgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAg > 1:988423/1‑100 (MQ=255) tgatgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAg > 2:1731483/1‑100 (MQ=255) gatgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGa > 2:1416303/1‑100 (MQ=255) atgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCTTTCGCCAGa > 1:907648/1‑99 (MQ=255) tgaGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGAt > 1:966353/1‑99 (MQ=255) gagagaTGATGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATcc > 1:1161690/1‑100 (MQ=255) agaTGATGCTCTTCAGGCATCGCCACAAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCgg < 1:686612/100‑1 (MQ=255) gatgatGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGc < 1:814857/100‑1 (MQ=255) gatgatGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGc > 2:1190773/1‑100 (MQ=255) gatgatGCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGc < 2:1790052/100‑1 (MQ=255) atgatgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCa < 1:107239/100‑1 (MQ=255) atgCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCACTCGCCAGATCCCGGCATCa < 1:808435/100‑1 (MQ=255) gCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAg < 1:1991719/100‑1 (MQ=255) gCTCTTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAg < 2:360993/100‑1 (MQ=255) tctTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGtt > 2:198647/1‑100 (MQ=255) ctTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTc > 2:482195/1‑100 (MQ=255) ctTCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTc > 1:530394/1‑100 (MQ=255) tCAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTcgc > 1:1914837/1‑100 (MQ=255) cAGGCATCGCCACCAGAAACGATGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTTGTTCTGTTGCCATTCGCCAGATCCCGGCATCAAGTTcgcg < 2:178290/100‑1 (MQ=255) cAGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTcgcg < 2:988412/100‑1 (MQ=255) aGGCATCGCCACCAGATACGACGATTCATGCAATCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcgg < 2:2145546/100‑1 (MQ=255) aGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTcgcg < 1:1841625/99‑1 (MQ=255) aGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcgg > 2:1808749/1‑100 (MQ=255) aGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcgg < 1:2141897/100‑1 (MQ=255) aGGCATCGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCgcgg < 1:727742/100‑1 (MQ=255) gCATCGCCACCAGAAACGTCGATTAATGCAAGCGAAAGCTGCTAAATCAAGTCGGTGGTTCTGTCGCAATTTGCCAGATCAAGGCATCAAGTTGgcgg < 2:1086203/98‑1 (MQ=255) cATCGCCACCAGAAATGACGATTGATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGTCATTCGCCAGATCCCGGCATCAAGTTCTCGGCGt > 2:1551465/1‑100 (MQ=255) cGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTcc > 1:763727/1‑100 (MQ=255) cGCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTcc < 2:551779/100‑1 (MQ=255) gCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCa > 1:915829/1‑100 (MQ=255) gCCACCAGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGCGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCa > 2:676574/1‑100 (MQ=255) ccaGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCaa > 1:46871/1‑100 (MQ=255) caGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAg > 1:1396914/1‑100 (MQ=255) aGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAgg > 2:412318/1‑100 (MQ=255) aGAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAgg < 1:476022/100‑1 (MQ=255) gAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGc < 2:218068/100‑1 (MQ=255) gAAACGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGc > 1:1015079/1‑100 (MQ=255) aaCGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCa < 1:1287837/100‑1 (MQ=255) aaCGACGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCa > 1:1125163/1‑100 (MQ=255) | GGCGGCATTGTAACAAAATACTCGTCACGTAGCGCTTCCAGCGGGACGGTGGAAAATGATGAGAGATGATGCTCTTCAGGCATCGCCACCAGAAACGCCGATTCATGCAACCGTAAGCTGGTAAAACCAGTCGGTGGTTCTGTCGCCATTCGCCAGATCCCGGCATCAAGTTCGCGGCGTTCCAGCAAGGCCA > NC_000913/2521870‑2522062 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |