Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,618,527 | A→T | 15.3% | W84R (TGG→AGG) | hda ← | ATPase regulatory factor involved in DnaA inactivation |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,618,527 | 0 | A | T | 15.3% | 220.1 / 29.0 | 85 | W84R (TGG→AGG) | hda | ATPase regulatory factor involved in DnaA inactivation |
Reads supporting (aligned to +/- strand): ref base A (43/29); new base T (9/4); total (52/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.58e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 |
CGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCG > NC_000913/2618429‑2618626 | cGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAg < 2:2026656/100‑1 (MQ=255) gCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAgg > 2:95160/1‑100 (MQ=255) gCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAgg > 1:402426/1‑100 (MQ=255) ccATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGt > 1:2057804/1‑100 (MQ=255) tcCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGtt < 2:1314056/100‑1 (MQ=255) tcCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGtt > 1:1522538/1‑100 (MQ=255) cccACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGttt > 1:2218220/1‑100 (MQ=255) aCTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATcc < 1:640686/98‑1 (MQ=255) tCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCg < 1:245317/100‑1 (MQ=255) cATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCcggcgg > 2:2149497/1‑100 (MQ=255) cATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCgg < 1:347701/100‑1 (MQ=255) cATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCgg > 2:762368/1‑100 (MQ=255) cATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCg < 2:605959/99‑1 (MQ=255) aTCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCggg < 2:2218194/100‑1 (MQ=255) tCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTGCGGAACAAACCTGGTGCGTTTATCCAGCGGGa < 1:2279631/100‑1 (MQ=255) tCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGa < 1:2006281/100‑1 (MQ=255) tCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGa < 2:1812751/100‑1 (MQ=255) gCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACa > 1:1751131/1‑100 (MQ=255) gCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACa > 1:1284314/1‑100 (MQ=255) ccTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACAt > 2:301135/1‑100 (MQ=255) ccTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACAt < 2:754495/100‑1 (MQ=255) cTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATa > 2:652105/1‑100 (MQ=255) gCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGc < 2:739302/100‑1 (MQ=255) gCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGc > 2:2217957/1‑100 (MQ=255) aaTACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGccc < 2:1495132/100‑1 (MQ=255) aaTACACTCAATGTTGTCGATAAAGACAAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCAAGCGGGACATAGccc > 2:828482/1‑100 (MQ=255) aTACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCa < 1:1363859/100‑1 (MQ=255) acTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCAcc > 2:784663/1‑98 (MQ=255) acTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGc > 1:682312/1‑100 (MQ=255) aaTGTTGTCGATACAGACCAGCGACAAATGCTCCATTCCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCAt < 2:1305486/98‑1 (MQ=255) tgttgtCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCTGGTGCGTTTATCCAGCGGGACATAGCTCACCGCATCGcc > 1:1179815/1‑100 (MQ=255) gttgtCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCa > 2:1987953/1‑100 (MQ=255) gttgtCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCa > 1:954051/1‑100 (MQ=255) gttgtCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCa > 1:2305304/1‑100 (MQ=255) ttgtCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCag < 2:1008386/100‑2 (MQ=255) ttgtCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCa < 1:668620/99‑1 (MQ=255) tgtCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACg > 2:1212410/1‑100 (MQ=255) tgtCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCAAGCGGGGCATAGCCCACCGCATCGCCa > 2:376708/1‑98 (MQ=255) cGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCt > 1:392371/1‑99 (MQ=255) cGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTg > 1:1324002/1‑100 (MQ=255) gATACAGACCAGTGCANAATGCTCCATACCGTANAGTCATTNCGTGNCCAGCCAGGTGGGTCAATCCAGCGGTGTATAGCCCACCACACCGCGACGCTGc > 2:2210209/1‑100 (MQ=255) aTACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCTGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGcn < 2:856739/100‑2 (MQ=255) aTACAGACCAGCGACAAATGCTCCATACCGTCAAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGCGACATAGCCCTCCGCATCGCCACGCTGc > 2:112136/1‑99 (MQ=255) tACAGACCATCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGa > 1:1638422/1‑100 (MQ=255) tACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCTGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCg > 1:40363/1‑99 (MQ=255) tACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGa < 2:776897/100‑1 (MQ=255) aCAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGAc < 1:968871/100‑1 (MQ=255) cAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACa > 2:1048868/1‑100 (MQ=255) gACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCTGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAAt > 2:763080/1‑100 (MQ=255) ccAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTc > 2:1788030/1‑100 (MQ=255) cAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCTGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTcc > 1:2070313/1‑100 (MQ=255) aCAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCa < 2:1733261/100‑1 (MQ=255) cAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCaa > 2:717787/1‑100 (MQ=255) aaaTGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAg < 2:1638402/100‑1 (MQ=255) aTGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGcc < 1:220025/100‑1 (MQ=255) cTCCATACCGTCGAGCACTTCCGGAACAAACCTGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCcgcg < 1:1570879/100‑1 (MQ=255) cTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCcgcg < 1:355354/100‑1 (MQ=255) tCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGt > 2:2233119/1‑100 (MQ=255) ccATACCGTCGAGCACTTCCGGAACAAACCTGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTg < 1:1716125/100‑1 (MQ=255) ccATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCcgcg > 1:1920684/1‑98 (MQ=255) cATACCGTCGAGCACTTCCGGAACAAACGTGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCTTgc > 1:1428111/1‑100 (MQ=255) aTACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTgca > 1:39112/1‑100 (MQ=255) tACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTgcag > 2:516797/1‑100 (MQ=255) ccGTCGAGCACTTCCGGAACAAACCTGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTgcagca > 2:1402035/1‑100 (MQ=255) cGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGcagcag > 1:2327911/1‑100 (MQ=255) gTCGAGCACTTCCGGAACAAACCTGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCACGCCGCGTGCAGCAGa > 1:2296860/1‑100 (MQ=255) cGAGCACTTCCGGAACAAACCTGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGCAGCAGATg > 2:1122153/1‑100 (MQ=255) aGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGCAGCAGATGGc < 2:1806859/100‑1 (MQ=255) aCTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTgc > 1:1439628/1‑100 (MQ=255) cTTCCGGAACAAACCTGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTgcg > 1:344476/1‑100 (MQ=255) cTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTgcg > 1:2311698/1‑100 (MQ=255) ttCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTgcgc > 1:2381364/1‑100 (MQ=255) ttCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTgcgc > 2:1813944/1‑100 (MQ=255) tCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGcc > 1:383634/1‑100 (MQ=255) ccGGAACAAACCAGGTGCTTTTATCCAGAGGGATATAGCCCATCTGATCGCTACGCTGCGACAATTCCGCGCAAGCCGTGTGCAGCAGATGGCTGCGccc < 2:2229136/100‑1 (MQ=255) cGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGcccc < 2:541765/100‑1 (MQ=255) atctaaCCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCcgcg < 1:1990083/96‑1 (MQ=255) aaCAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCcgcg < 1:132651/100‑1 (MQ=255) aCAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCgcgc > 1:1408507/1‑100 (MQ=255) aCAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCgcgc > 1:1071049/1‑100 (MQ=255) cAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGcc > 2:289918/1‑100 (MQ=255) cAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGcc > 1:2094340/1‑100 (MQ=255) aaCCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCcgcgag > 2:1990061/1‑96 (MQ=255) ccAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTcg < 1:2025114/100‑1 (MQ=255) ccAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTcg < 1:1359615/100‑1 (MQ=255) aGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTcgcg > 2:1351542/1‑100 (MQ=255) | CGCCATCTCCCACAACTCATCGCCTGCAATACACTCAATGTTGTCGATACAGACCAGCGACAAATGCTCCATACCGTCGAGCACTTCCGGAACAAACCAGGTGCGTTTATCCAGCGGGACATAGCCCACCGCATCGCCACGCTGCGACAATTCCGCGCAAGCCGCGTGCAGCAGATGGCTGCGCCCCGCGCCTTCGCG > NC_000913/2618429‑2618626 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |