Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,782,883 G→A 59.8% intergenic (‑157/+182) ypjA ← / ← pinH adhesin‑like autotransporter/pseudogene, invertase resolvase family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,782,8830GA59.8% 54.1 / 117.4 102intergenic (‑157/+182)ypjA/pinHadhesin‑like autotransporter/pseudogene, invertase resolvase family
Reads supporting (aligned to +/- strand):  ref base G (24/17);  new base A (34/27);  total (58/44)
Fisher's exact test for biased strand distribution p-value = 8.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 112 total aligned reads displayed.

TGCAACATCAGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCT  >  NC_000913/2782784‑2782982
                                                                                                   |                                                                                                   
tgcAACATCAGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAatg                                                                                                     >  2:2228954/1‑100 (MQ=255)
tgcAACATCAGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAatg                                                                                                     >  1:358203/1‑100 (MQ=255)
 gcAACATCAGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAatga                                                                                                    >  1:984576/1‑100 (MQ=255)
  cAACATCAGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAaataat                                                                                                   >  2:1354827/1‑100 (MQ=255)
   aaCATCAGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAatgat                                                                                                   <  1:1986310/99‑1 (MQ=255)
   aaCATCAGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAataatg                                                                                                  >  2:1195460/1‑100 (MQ=255)
     cATCAGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATgatgat                                                                                                <  1:2247828/100‑1 (MQ=255)
     cATCAGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATgatgat                                                                                                <  2:1039370/100‑1 (MQ=40)
     cATCAGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATaatgat                                                                                                <  2:738257/100‑1 (MQ=255)
      aTCAGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGata                                                                                               <  1:1544342/100‑1 (MQ=255)
       tCAGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGatat                                                                                              <  2:2037958/100‑1 (MQ=255)
         aGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTc                                                                                            <  2:12631/100‑1 (MQ=255)
          gcgcacaGGCACCAGACCAGGGGAATCATACTGAAGAGACAGCGCAAGTGTATTGTGTTCAACGCACATCAAAGAAATCATGATGAAATAATGATAttt                                                                                            >  2:2076860/4‑98 (MQ=14)
          gCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCGCCGCTCATCAAAGACATCATGATGAAATAATGATATTcc                                                                                           >  1:1520309/1‑100 (MQ=255)
          gCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTcc                                                                                           >  2:1735339/1‑100 (MQ=255)
            ccACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGc                                                                                         <  2:1825435/100‑1 (MQ=255)
             cacaGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCa                                                                                        >  1:4184/1‑100 (MQ=255)
               caGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTTTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATa                                                                                      <  2:1053186/100‑1 (MQ=255)
                aGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATaa                                                                                     <  2:2349318/100‑1 (MQ=255)
                 ggCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAg                                                                                    >  1:2403967/1‑100 (MQ=255)
                   cACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGaa                                                                                  >  2:1836072/1‑100 (MQ=255)
                     ccagaccagGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATg                                                                                <  2:1087882/100‑1 (MQ=255)
                     ccagaccagGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATg                                                                                >  2:1240559/1‑100 (MQ=255)
                      cagaccagGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGa                                                                               <  2:838334/100‑1 (MQ=255)
                       agaccagGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAg                                                                              <  1:1118940/100‑1 (MQ=255)
                        gaccagGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAgg                                                                             <  1:276331/100‑1 (MQ=255)
                        gaccagGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAgg                                                                             <  2:2288205/100‑1 (MQ=255)
                        gaccagGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAgg                                                                             >  1:459498/1‑100 (MQ=255)
                         accagGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGc                                                                            >  1:1388506/1‑100 (MQ=255)
                          ccagGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGc                                                                            <  2:1046019/99‑1 (MQ=255)
                          ccagGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCa                                                                           >  2:2203997/1‑100 (MQ=255)
                             ggggATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCAttt                                                                        <  1:1240572/100‑1 (MQ=255)
                               ggATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCAttttt                                                                      <  1:713514/100‑1 (MQ=255)
                               ggATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCAttttt                                                                      <  2:1718342/100‑1 (MQ=255)
                                gATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCAtttttt                                                                     <  2:1343352/100‑1 (MQ=255)
                                 aTTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTa                                                                    <  2:68925/100‑1 (MQ=255)
                                  ttCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTaa                                                                   <  2:2130706/100‑1 (MQ=255)
                                  ttCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTaa                                                                   <  2:2149412/100‑1 (MQ=255)
                                    cATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAAc                                                                 >  2:526182/1‑100 (MQ=255)
                                    cATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAAc                                                                 >  1:424595/1‑100 (MQ=255)
                                     aTCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAAc                                                                 >  1:399342/1‑99 (MQ=255)
                                      tCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGc                                                               >  2:2196595/1‑100 (MQ=255)
                                      tCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGc                                                               >  1:834850/1‑100 (MQ=255)
                                         tGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGt                                                            <  1:1817403/100‑1 (MQ=255)
                                          gAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTg                                                           >  2:296922/1‑100 (MQ=255)
                                               gaCAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTg                                                      >  1:1574505/1‑100 (MQ=255)
                                                aCAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATGTTTTAAACGCAGTGCGCTg                                                      <  2:535501/99‑1 (MQ=255)
                                                 cAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGaa                                                    >  1:364355/1‑100 (MQ=255)
                                                  aGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGTCATTTTTTAAACGCAGTGCGCTGAAg                                                   <  1:1457746/100‑1 (MQ=255)
                                                  aGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAg                                                   <  1:306378/100‑1 (MQ=255)
                                                  aGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAg                                                   <  1:958288/100‑1 (MQ=255)
                                                  aGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAg                                                   <  1:2316452/100‑1 (MQ=255)
                                                         gtgtATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGtt                                            <  1:2057447/100‑1 (MQ=255)
                                                          tgtATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTg                                           <  1:686069/100‑1 (MQ=255)
                                                           gtATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTgg                                          <  2:834841/100‑1 (MQ=255)
                                                             aTTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGAt                                        >  1:2157590/1‑100 (MQ=255)
                                                             aTTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGAt                                        >  1:1488567/1‑100 (MQ=255)
                                                             aTTGTGTTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGAt                                        >  2:2369407/1‑100 (MQ=255)
                                                              ttGTGTTCACCGCTAATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGCTTGGATa                                       <  1:1259805/100‑1 (MQ=255)
                                                                gtgtTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATaaa                                     >  2:1023535/1‑100 (MQ=255)
                                                                  gtTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATaaaaa                                   >  1:2079888/1‑100 (MQ=255)
                                                                  gtTCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATaaaaa                                   <  2:459495/100‑1 (MQ=255)
                                                                   ttCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAATGCAGTGCTCTGAAGTGTGGTTGGATaaa                                     >  2:626455/1‑97 (MQ=255)
                                                                    tCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAg                                 >  2:761997/1‑100 (MQ=255)
                                                                    tCACCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGt                                >  1:623242/1‑100 (MQ=255)
                                                                     cACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGt                                >  1:1311238/1‑100 (MQ=255)
                                                                      aCCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTc                               <  2:1557760/100‑1 (MQ=255)
                                                                      aCCGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTc                               <  2:1657220/100‑1 (MQ=255)
                                                                       ccGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCa                              >  2:2385106/1‑100 (MQ=255)
                                                                       ccGCTCATCAAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCACTGAAGTGTGGTTGGATAAAAAAGTc                               <  1:2251967/99‑1 (MQ=255)
                                                                        cGCTCATCAAAGACATCATGATGAAATAATGATATTACGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCaa                             >  1:1687250/1‑100 (MQ=255)
                                                                          cTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATc                           <  1:541628/100‑1 (MQ=255)
                                                                            catcaAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCa                         >  1:2245616/1‑100 (MQ=255)
                                                                            catcaAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCa                         >  1:1493656/1‑100 (MQ=255)
                                                                            catcaAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCa                         <  1:1257464/100‑1 (MQ=255)
                                                                            catcaAAGACATAATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCa                         >  1:1571222/1‑100 (MQ=255)
                                                                            catcaAACACACCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTACGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCa                         >  2:1257451/1‑100 (MQ=255)
                                                                              tcaAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCAtt                       >  1:1323134/1‑100 (MQ=255)
                                                                               caAAGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTc                      >  2:1736546/1‑100 (MQ=255)
                                                                                aaaGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCa                     <  1:1487955/100‑1 (MQ=255)
                                                                                aaaGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCa                     >  2:1105840/1‑100 (MQ=255)
                                                                                aaaGACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCa                     <  2:1787908/100‑1 (MQ=255)
                                                                                  aGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAgg                   >  1:1378137/1‑100 (MQ=255)
                                                                                   gACATCATGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGa                  <  1:218317/100‑1 (MQ=255)
                                                                                     catcatGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGaaa                <  1:508450/100‑1 (MQ=255)
                                                                                       tcatGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATa              >  2:695048/1‑100 (MQ=255)
                                                                                        catGATGAAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATAc             >  1:2398455/1‑100 (MQ=255)
                                                                                        catGATGAAATAATGATATTCCGAATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATAc             >  2:1353755/1‑100 (MQ=255)
                                                                                          tgatgaAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACgg           >  2:849701/1‑100 (MQ=255)
                                                                                          tgatgaAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGAAAATACgg           >  1:2016306/1‑100 (MQ=255)
                                                                                          tgatgaAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTTAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATAc             >  1:179181/1‑98 (MQ=255)
                                                                                          tgatgaAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACgg           >  1:1056873/1‑100 (MQ=255)
                                                                                           gatgaAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCAATTCAGGAAATACggg          >  1:1906909/1‑100 (MQ=255)
                                                                                            atgaAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGc         >  1:358769/1‑100 (MQ=255)
                                                                                             tgaAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCg        >  1:618641/1‑100 (MQ=255)
                                                                                             tgaAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCg        <  2:2168110/100‑1 (MQ=255)
                                                                                              gaAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGt       <  1:493292/100‑1 (MQ=255)
                                                                                              gaAATAATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTAAGGACATATGGGCGt       >  2:1998486/1‑100 (MQ=21)
                                                                                               aaaTGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTa      >  1:999848/1‑100 (MQ=255)
                                                                                                   gatgatATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTAttct  <  2:1311224/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
TGCAACATCAGCCCACAGGCACCAGACCAGGGGATTCATCCTGAAGAGACAGCGCAAGTGTATTGTGTTCACCGCTCATCAAAGACATCATGATGAAATGATGATATTCCGCATAAGAATGAGGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCT  >  NC_000913/2782784‑2782982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: