Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,810,758	G→A	60.6%	intergenic (+79/‑12)	ygaH → / → mprA	putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,810,758	0	G	A	60.6%	59.8 / 99.9	99	intergenic (+79/‑12)	ygaH/mprA	putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base G (21/18);  new base A (30/30);  total (51/48)
Fisher's exact test for biased strand distribution p-value = 8.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

AAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTG  >  NC_000913/2810660‑2810856                                                                                                   |                                                                                                   aaGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAgg                                                                                                   <  1:1695816/100‑1 (MQ=255)    tGATGGCGATTATATAACCCACAAGAATTATTTTTCTAAAACAATACATTTACTTTATTTGTCATTGTCGTTACTATATTGGTTGAAATTAATAAGGTCa                                                                                                <  2:2044474/100‑1 (MQ=255)    tGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCa                                                                                                >  1:240077/1‑100 (MQ=255)    tGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCa                                                                                                >  2:265910/1‑100 (MQ=255)    tGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCa                                                                                                <  1:1372494/100‑1 (MQ=255)    tGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCa                                                                                                <  1:1052178/100‑1 (MQ=255)    tGATGGCGATTATATAACACACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTc                                                                                                 <  2:375922/99‑1 (MQ=255)      tcttccgatctataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATa                                                                                              <  1:1057193/90‑1 (MQ=255)      aTGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATa                                                                                              >  1:885644/1‑100 (MQ=255)      aTGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATa                                                                                              <  1:263795/100‑1 (MQ=255)      aTGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACACTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATa                                                                                              >  2:1330315/1‑100 (MQ=255)       tGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATAc                                                                                             >  2:1703442/1‑100 (MQ=255)          cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCa                                                                                          >  1:1991263/1‑100 (MQ=255)          cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCa                                                                                          >  1:2279807/1‑100 (MQ=255)              tatataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATg                                                                                      <  1:462119/100‑1 (MQ=255)               atataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATgg                                                                                     >  1:401329/1‑100 (MQ=255)                tataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGa                                                                                    >  2:154864/1‑100 (MQ=255)                  taACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTGATAAAGTCATACCCAAATGGATa                                                                                  >  2:623957/1‑100 (MQ=255)                  taACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATa                                                                                  >  2:515233/1‑100 (MQ=255)                   aaCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAg                                                                                 <  2:2021664/100‑1 (MQ=255)                   aaCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAg                                                                                 >  2:52452/1‑100 (MQ=255)                   aaCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAg                                                                                 <  1:1101966/100‑1 (MQ=255)                    aCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGt                                                                                >  2:2372873/1‑100 (MQ=255)                     cccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGtt                                                                               >  2:2350980/1‑100 (MQ=255)                       cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCTTCTGAATTTAATAAGTTCATACCCAAATGGATAGTTc                                                                              >  1:1553169/1‑99 (MQ=255)                       cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCg                                                                             <  2:487153/100‑1 (MQ=255)                       cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCg                                                                             <  2:160579/100‑1 (MQ=255)                        acaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCg                                                                             <  1:684733/99‑1 (MQ=255)                         caAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGtt                                                                           >  2:1835881/1‑100 (MQ=255)                         caAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGtt                                                                           >  2:504790/1‑100 (MQ=255)                         caAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGtt                                                                           <  2:828147/100‑1 (MQ=255)                           aGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGCCATACCCAAATGGATAGTTCGTTTa                                                                         <  1:471757/100‑1 (MQ=255)                             aaTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACg                                                                       <  1:1548614/100‑1 (MQ=255)                               tCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGcc                                                                     <  1:509705/100‑1 (MQ=255)                               tCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGcc                                                                     <  2:913360/100‑1 (MQ=255)                                cATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTTAGccc                                                                    >  2:1910934/1‑100 (MQ=255)                                cATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGc                                                                      >  2:2126402/1‑98 (MQ=255)                                cATTTTTCTAAAACAATACATTTAATTTAATTGTCAATGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTAc                                                                        >  2:1048067/1‑96 (MQ=255)                                   ttttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCAtt                                                                 <  1:1908271/100‑1 (MQ=255)                                    tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGTCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                                <  1:1163199/100‑1 (MQ=255)                                    tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTg                                                                <  2:1164437/100‑1 (MQ=255)                                       cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                             <  1:693856/100‑1 (MQ=255)                                       cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                             >  2:2366872/1‑100 (MQ=255)                                        tAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACa                                                            >  2:119202/1‑100 (MQ=255)                                        tAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACa                                                            <  1:82482/100‑1 (MQ=255)                                         aaaaCAATACATTTACTTTATTTGTCAGTGTCGTTACTATATCGGCTGAAATTAATTAAGTCATACCCACATGGCTAGTTCGTTTACACCCATTGAAc                                                             >  2:904573/1‑98 (MQ=255)                                           aaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAAt                                                         <  2:679297/100‑1 (MQ=255)                                           aaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAAt                                                         <  1:1142773/100‑1 (MQ=255)                                           aaCAATACATTTACTTTATTTGTCACTGTAGTTACTATATCGGCTAAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAAt                                                         <  2:1303505/100‑1 (MQ=255)                                             cAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGc                                                       >  2:1414674/1‑100 (MQ=255)                                             cAATACATTTACTTTATTTGTCACTGTCGTTACTATACCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGc                                                       <  1:373735/100‑1 (MQ=255)                                                tACATTTAATTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaa                                                    <  1:407973/100‑1 (MQ=255)                                                 aCATATATTTTATTTGTCACTGTCGTTGATATATCGAATGAAATTAATAAAGTCATACCCAAATGGATAGTTCTTTTACGCCTATTGAACaaagagtaaa                                                   <  2:78948/100‑8 (MQ=255)                                                  cATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaaa                                                  >  1:927863/1‑100 (MQ=255)                                                  cATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaaa                                                  >  2:1194216/1‑100 (MQ=255)                                                   aTTTACTTTATTTGTCACTGTCGTTGCTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAt                                                 >  1:578980/1‑100 (MQ=255)                                                   aTTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAt                                                 >  1:622376/1‑100 (MQ=255)                                                   aTTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAt                                                 >  2:611498/1‑100 (MQ=255)                                                      tACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAACTGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAAtt                                                >  2:1683869/1‑98 (MQ=255)                                                      tACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAACTGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTc                                              >  2:1683850/1‑100 (MQ=255)                                                       aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                             >  1:566346/1‑100 (MQ=255)                                                       aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                             <  2:1695540/100‑1 (MQ=255)                                                        cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                            <  2:974526/100‑1 (MQ=255)                                                        cTTTATTTGTCACTGTCGTTACTATACCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                            <  2:1930511/100‑1 (MQ=255)                                                         tttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgcg                                           <  2:2128121/100‑1 (MQ=255)                                                         tttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgcg                                           <  2:1383788/100‑1 (MQ=255)                                                          ttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCgcgc                                          <  2:410595/100‑1 (MQ=255)                                                           tATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGcc                                         <  1:1544806/100‑1 (MQ=255)                                                           tATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGcc                                         >  1:495525/1‑100 (MQ=255)                                                            aTTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCa                                        >  2:629966/1‑100 (MQ=255)                                                            aTTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCa                                        >  1:690458/1‑100 (MQ=255)                                                             tttGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAg                                       >  2:995230/1‑100 (MQ=255)                                                               tGTCACTGTTGTTAATATAAAGGATGTAATGAATAAAGTAATAACCAAATGGATAGTTCGATTACGAACAATGAATAAATGTTAAAATTTcgcacga                                        >  2:1567038/1‑93 (MQ=255)                                                                  cACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccgcc                                  >  1:2177865/1‑100 (MQ=255)                                                                   aCTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCa                                 >  2:660606/1‑100 (MQ=255)                                                                       tCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGaa                             <  2:1270283/100‑1 (MQ=255)                                                                         gTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGa                           <  1:569983/100‑1 (MQ=255)                                                                         gTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGa                           >  2:208267/1‑100 (MQ=255)                                                                          ttACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAt                          <  2:1602138/100‑1 (MQ=255)                                                                           tACTATATCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtt                         >  2:1943703/1‑100 (MQ=255)                                                                           tACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGAtt                         >  2:1826026/1‑100 (MQ=255)                                                                               atatCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTCTcc                     >  1:2102825/1‑100 (MQ=255)                                                                               atatCGGCTGAAATTAATAAAGTCATACCCAAATTGATAGTTCGTTTACGCCCATTGATCAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                     <  2:1498437/100‑1 (MQ=255)                                                                               atatCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGTACAAATGCTACAATTTCGCGCCAGCCGCTACGAAGAtttttc                     <  1:1616701/100‑3 (MQ=255)                                                                               atatCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                     >  2:287134/1‑100 (MQ=255)                                                                                tatCGGCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCt                    <  1:2351008/100‑1 (MQ=255)                                                                                tatCGGCTGAAATTAATAAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCt                    <  1:263114/100‑1 (MQ=255)                                                                                tatCGGCTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCt                    >  1:2199043/1‑100 (MQ=255)                                                                                 atCGGCTGAAATTAATAAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCtt                   >  2:238848/1‑100 (MQ=255)                                                                                    ggCTGAAATTAATAAGGCCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc                >  2:2218247/1‑100 (MQ=255)                                                                                     gCTGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCa               <  2:2340448/100‑1 (MQ=255)                                                                                      cTGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAg              <  2:70614/100‑1 (MQ=255)                                                                                       tGAAATTAATAAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg             >  1:2311796/1‑100 (MQ=255)                                                                                       tGAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg             <  2:924525/100‑1 (MQ=255)                                                                                        gAAATTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGga            >  1:1400516/1‑100 (MQ=255)                                                                                         aaaTTAATAAAGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGga            <  2:2168342/99‑1 (MQ=255)                                                                                             taataaAGTCATACCCAAATGGATAGTTCGTTTACGCACATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTACTTATCAGGAGATcc       >  2:989447/1‑100 (MQ=255)                                                                                                taaGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTc    <  1:675880/98‑1 (MQ=255)                                                                                                 aaGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCt   <  1:909427/99‑1 (MQ=255)                                                                                                  aGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTg  >  1:2352009/1‑100 (MQ=255)                                                                                                   |                                                                                                   AAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTG  >  NC_000913/2810660‑2810856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑