Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,862,917 T→G 18.7% Y195D (TAC→GAC)  ygbK → FliA‑regulated DUF1537 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,862,9170TG18.7% 240.9 / 54.9 107Y195D (TAC→GAC) ygbKFliA‑regulated DUF1537 family protein
Reads supporting (aligned to +/- strand):  ref base T (43/44);  new base G (12/8);  total (55/52)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

Only 100 of 108 total aligned reads displayed.

CCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTAACGGGCGGTTCTGGTCTGGCGATTG  >  NC_000913/2862819‑2863016
                                                                                                  |                                                                                                   
ccACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTa                                                                                                    >  2:339968/1‑100 (MQ=255)
 cacaGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTAc                                                                                                   >  1:1272422/1‑100 (MQ=255)
 cacaGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTAc                                                                                                   >  1:313821/1‑100 (MQ=255)
   caGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTAcgc                                                                                                 <  1:1736839/100‑1 (MQ=255)
     gggCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTAcgcg                                                                                                <  1:373116/99‑1 (MQ=255)
     gggCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCgg                                                                                               <  1:529413/100‑1 (MQ=255)
      ggCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGt                                                                                              >  2:2268118/1‑100 (MQ=255)
        cgcTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGc                                                                                            <  1:2302900/100‑1 (MQ=255)
         gcTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACTCGGTGCt                                                                                           <  1:1258176/100‑1 (MQ=255)
         gcTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCt                                                                                           >  1:560099/1‑100 (MQ=255)
         gcTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCt                                                                                           <  1:799923/100‑1 (MQ=255)
         gcTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCt                                                                                           >  2:667285/1‑100 (MQ=255)
          cTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCtt                                                                                          >  2:1384519/1‑100 (MQ=255)
           tGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTg                                                                                         <  2:861422/100‑1 (MQ=255)
            gcggcgTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGa                                                                                        <  1:806371/100‑1 (MQ=255)
             cggcgTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGAt                                                                                       <  2:1210086/100‑1 (MQ=255)
                 ggcgtTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATgcgc                                                                                   <  2:1455744/98‑1 (MQ=255)
                    gtTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGa                                                                                >  2:2045046/1‑100 (MQ=255)
                     tttCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGAc                                                                               >  1:2155933/1‑100 (MQ=255)
                     tttCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCGACGCGGTGCTTGATGCGCTGAc                                                                               <  1:2190015/100‑1 (MQ=255)
                      ttCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGAcc                                                                              <  1:2199734/100‑1 (MQ=255)
                      ttCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCGACGCGGTGCTTGATGCGCTGAcc                                                                              <  1:1767942/100‑1 (MQ=255)
                      ttCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCGACGCGGTGCTTGATGCGCTGAcc                                                                              <  2:1127388/100‑1 (MQ=255)
                       tCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGAcc                                                                              >  2:1995013/1‑99 (MQ=255)
                       tCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCg                                                                             <  2:572573/100‑1 (MQ=255)
                        cGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTATCGCTAGGCGGTGCTTGATGCGCTGcccta                                                                            >  2:1376957/1‑98 (MQ=255)
                         ggCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCGACGCGGTGCTTGATGCGCTGACCGaa                                                                           >  2:2162800/1‑100 (MQ=255)
                          gCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAac                                                                          <  2:102926/100‑1 (MQ=255)
                          gCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCGATGCGGTGCTTGATGCGCTGACCGAac                                                                          >  2:2373358/1‑100 (MQ=255)
                           cacaTGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAaca                                                                         <  2:1443770/100‑1 (MQ=255)
                           cacaTGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAaca                                                                         >  1:1946814/1‑100 (MQ=255)
                              aTGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCGACGCGGTGCTTGATGCGCTGACCGAACACCa                                                                      >  2:1464183/1‑100 (MQ=255)
                               tGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCa                                                                      >  2:1266884/1‑99 (MQ=255)
                                gTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATc                                                                    >  2:1201408/1‑100 (MQ=255)
                                gTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAAAACCATc                                                                    >  2:2085957/1‑100 (MQ=255)
                                  tttCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCGACGCGGTGCTTGATGCGCTGACCGAACACCATCTg                                                                  >  1:763056/1‑100 (MQ=255)
                                   ttCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTgg                                                                 >  1:1974438/1‑100 (MQ=255)
                                     cGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGAACGAACACCATCTGGaa                                                               >  1:1034795/1‑100 (MQ=255)
                                      gAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGaaa                                                              <  1:1950034/100‑1 (MQ=255)
                                      gAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGaaa                                                              >  1:1660028/1‑100 (MQ=255)
                                      gAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCGACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGaaa                                                              >  2:1716482/1‑100 (MQ=255)
                                       aaCAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAAt                                                             <  1:2164089/100‑1 (MQ=255)
                                       aaCAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAAt                                                             <  2:392513/100‑1 (MQ=255)
                                           aGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCa                                                          >  1:1359306/1‑99 (MQ=255)
                                           aGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAg                                                         <  1:339971/100‑1 (MQ=255)
                                           aGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAg                                                         >  2:320456/1‑100 (MQ=255)
                                            ggTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAgg                                                        <  1:483804/100‑1 (MQ=255)
                                            ggTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAgg                                                        <  1:1263450/100‑1 (MQ=255)
                                            ggTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAgg                                                        <  1:1384533/100‑1 (MQ=255)
                                            ggTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCGACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAgg                                                        <  1:2380603/100‑1 (MQ=255)
                                            ggTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCGACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAgg                                                        <  1:642722/100‑1 (MQ=255)
                                             gtgtGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAggg                                                       <  1:2049857/100‑1 (MQ=255)
                                              tgtgGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCGATGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGga                                                      <  1:2373386/100‑1 (MQ=255)
                                               gtgGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGgag                                                     <  1:125299/100‑1 (MQ=255)
                                                 ggATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCGACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGaa                                                   <  2:763047/100‑1 (MQ=255)
                                                  gATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAg                                                  <  2:560096/100‑1 (MQ=255)
                                                  gATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCGACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAg                                                  >  2:183815/1‑100 (MQ=255)
                                                   aTGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGc                                                 >  1:1516215/1‑100 (MQ=255)
                                                    tGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGcc                                                >  2:1055454/1‑100 (MQ=255)
                                                       cGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTg                                             >  1:1365532/1‑100 (MQ=255)
                                                           cGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGcgcg                                         >  2:58830/1‑100 (MQ=255)
                                                           cGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGcgcg                                         <  1:1583641/100‑1 (MQ=255)
                                                             tCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGAt                                       >  2:1870375/1‑100 (MQ=255)
                                                              cAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCGACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATg                                      >  2:1360918/1‑100 (MQ=255)
                                                              cAAGAGCTGGCTCGCTTACAGAAAGAGGGGTACCGCTACGCGGTGCTTGATGCGCTGAACGAACACCTTATGGAAATTCAGGGAGAAGCCTTGCGCGATg                                      >  2:902784/1‑100 (MQ=255)
                                                               aaGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTACGCGATGc                                     >  2:1795548/1‑100 (MQ=255)
                                                                agagCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGcc                                    <  1:1814750/100‑1 (MQ=255)
                                                                agagCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGcc                                    <  1:393568/100‑1 (MQ=255)
                                                                 gagCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGccc                                   >  1:1053932/1‑100 (MQ=255)
                                                                 gagCTGGCTCGCTTACAGCAAGAGGGCTACCGCGACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGccc                                   >  2:941247/1‑100 (MQ=255)
                                                                   gCTGGCTCGCTTACAGCAAGAGTGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGcccc                                  <  1:1332942/99‑1 (MQ=255)
                                                                   gCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCa                                 <  2:100819/100‑1 (MQ=255)
                                                                    cTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCAc                                <  1:2378539/100‑1 (MQ=255)
                                                                      ggCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTg                              >  1:555350/1‑100 (MQ=255)
                                                                      ggCTCGCTTACAGCAAGAGGGCTACCGCGACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTg                              <  1:1716502/100‑1 (MQ=255)
                                                                      ggCTCGCTTACAGCAAGAGGGCTACCGCGACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGAAGCCCCACt                               >  1:203455/1‑99 (MQ=255)
                                                                        cTCGCTTACAGCAAGAGAAATACCGATACGCGATGCATGATGCGCTGAACGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACt                               >  2:509114/1‑97 (MQ=255)
                                                                         tCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTa                           <  1:1379470/100‑1 (MQ=255)
                                                                         tCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTa                           >  1:1945722/1‑100 (MQ=255)
                                                                           gCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTAAc                         >  2:1238306/1‑100 (MQ=255)
                                                                            cTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTAACg                        >  2:1762375/1‑100 (MQ=255)
                                                                              tACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTAACggg                      <  1:908739/100‑1 (MQ=255)
                                                                              tACAGCAAGAGGGCTACCGCGACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTAACggg                      >  1:1771877/1‑100 (MQ=255)
                                                                                cAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTAACGGGCg                    >  2:340968/1‑100 (MQ=255)
                                                                                cAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACAATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTAACGGGCg                    >  2:2104604/1‑100 (MQ=255)
                                                                                 aGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTAACGGGCgg                   >  2:454625/1‑100 (MQ=255)
                                                                                  gCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTAACGGGCgg                   >  2:1645387/1‑99 (MQ=255)
                                                                                  gCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTAACGGGCGGt                  <  1:628426/100‑1 (MQ=255)
                                                                                  gCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTAACGGGCGGt                  <  1:1394182/100‑1 (MQ=255)
                                                                                  gCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTAACGGGCGGt                  >  2:2289414/1‑100 (MQ=255)
                                                                                   cAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTAACGGGCGGtt                 >  1:1852158/1‑100 (MQ=255)
                                                                                       agGGCTACCGCTACGCGGTGCTTGATGCGCGGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTAACGGGCGGTtctgg             <  1:2215411/100‑1 (MQ=255)
                                                                                        gggCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTAACGGGCGGTtctggt            >  2:58483/1‑100 (MQ=255)
                                                                                        gggCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTAACGGGCGGTtctggt            >  2:639574/1‑100 (MQ=255)
                                                                                         ggCTACGGGTACGGGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTAACGGGCGGTtctggtc           <  1:1561997/100‑1 (MQ=255)
                                                                                             aCCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTAACGGGCGGTTCTGGTCTGGc       <  1:1523837/100‑1 (MQ=255)
                                                                                             aCCGCGACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTAACGGGCGGTTCTGGTCTGGc       >  2:1132094/1‑100 (MQ=255)
                                                                                              ccGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTAACGGGCGGTTCTGGTCTGGCg      <  2:535496/100‑1 (MQ=255)
                                                                                                 cTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTAACGGGCGGTTCTGGTCTGGCGAtt   <  1:1875605/100‑1 (MQ=255)
                                                                                                  tACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTAACGGGCGGTTCTGGTCTGGCGATtg  <  1:135778/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
CCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGCAAGAGGGCTACCGCTACGCGGTGCTTGATGCGCTGACCGAACACCATCTGGAAATTCAGGGAGAAGCCTTGCGCGATGCCCCACTGGTAACGGGCGGTTCTGGTCTGGCGATTG  >  NC_000913/2862819‑2863016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: