Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 364,024 C→T 15.6% R761H (CGC→CAC)  lacZ ← beta‑D‑galactosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913364,0240CT15.6% 239.4 / 24.8 95R761H (CGC→CAC) lacZbeta‑D‑galactosidase
Reads supporting (aligned to +/- strand):  ref base C (39/41);  new base T (12/3);  total (51/44)
Fisher's exact test for biased strand distribution p-value = 4.57e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

ATGTCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACAC  >  NC_000913/363925‑364123
                                                                                                   |                                                                                                   
aTGTCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGc                                                                                                     <  2:1127986/100‑1 (MQ=255)
aTGTCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGc                                                                                                     <  1:209384/100‑1 (MQ=255)
aTGTCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGc                                                                                                     <  2:348058/100‑1 (MQ=255)
   tCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGACTGACTGGc                                                                                                     >  2:1837998/1‑97 (MQ=255)
    cGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGtt                                                                                                 <  1:144261/100‑1 (MQ=255)
     gTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTa                                                                                                <  2:828855/100‑1 (MQ=255)
     gTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTa                                                                                                >  2:256468/1‑100 (MQ=255)
       tATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATTTGGGAAAGAAAGCCTGACTGGCGCTTaaa                                                                                              >  2:733559/1‑100 (MQ=255)
        aTCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAAt                                                                                             >  1:2293058/1‑100 (MQ=255)
         tCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAAtt                                                                                            >  2:906220/1‑100 (MQ=255)
         tCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAAtt                                                                                            <  1:1094773/100‑1 (MQ=255)
          ccAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTg                                                                                           <  1:1761020/100‑1 (MQ=255)
           cAGCGGTGCACGGGTGAAGTGATCGAGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTg                                                                                           <  1:212753/99‑1 (MQ=255)
           cAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGc                                                                                          <  2:1610233/100‑1 (MQ=255)
           cAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGc                                                                                          >  1:239604/1‑100 (MQ=255)
           cAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGc                                                                                          >  2:1720054/1‑100 (MQ=255)
           cAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGc                                                                                          <  2:2134814/100‑1 (MQ=255)
           cAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGc                                                                                          >  1:1804074/1‑100 (MQ=255)
           cAGCGGTGCACGGGTGAAATGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTg                                                                                           >  2:1103425/1‑99 (MQ=255)
            aGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGcc                                                                                         <  2:2190110/100‑1 (MQ=255)
             gCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGGCAATCCACATCCGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCa                                                                                        <  2:1776728/100‑1 (MQ=255)
             ctggTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCa                                                                                        <  2:2049693/98‑1 (MQ=255)
               ggTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCaag                                                                                      >  1:2049715/1‑99 (MQ=255)
               ggTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAAc                                                                                      <  1:534411/100‑1 (MQ=255)
                gTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACg                                                                                     >  2:1090428/1‑100 (MQ=255)
                      gggTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCttatt                                                                               <  2:1290256/100‑1 (MQ=255)
                        gTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTAc                                                                             <  1:992557/100‑1 (MQ=255)
                          gAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTAccc                                                                           >  2:1784218/1‑100 (MQ=255)
                            aCTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAg                                                                         <  1:1137014/100‑1 (MQ=255)
                            aCTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAg                                                                         <  1:1299308/100‑1 (MQ=255)
                             cTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGc                                                                        <  1:1795649/100‑1 (MQ=255)
                             cTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGc                                                                        >  2:848470/1‑100 (MQ=255)
                             cTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGc                                                                        >  1:714909/1‑100 (MQ=255)
                               gATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTc                                                                      >  2:1662015/1‑100 (MQ=255)
                               gATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTc                                                                      >  2:2063637/1‑100 (MQ=255)
                               gATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTc                                                                      >  2:2062656/1‑100 (MQ=255)
                                aTCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCg                                                                     >  2:1205304/1‑100 (MQ=255)
                                aTCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCg                                                                     >  2:266519/1‑100 (MQ=255)
                                 tCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGa                                                                    <  2:1804053/100‑1 (MQ=255)
                                   gcgcAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATg                                                                  <  1:1734406/100‑1 (MQ=255)
                                    cgcAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGTTTAAATTGCCAACGCTTATTACCCAGCTCGATg                                                                  <  2:923609/99‑1 (MQ=255)
                                    cgcAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGc                                                                 <  1:256471/100‑1 (MQ=255)
                                        gtggcgTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCaaaa                                                             >  1:573224/3‑100 (MQ=255)
                                        gcggcgTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCaaaa                                                             >  1:989949/1‑100 (MQ=255)
                                         cggcgTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCaaaaa                                                            <  1:1755807/100‑1 (MQ=255)
                                           gcgTCATCAGTTGTTTTTTATCTCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATc                                                          <  2:2112331/100‑1 (MQ=255)
                                            cgTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATcc                                                         <  1:1490472/100‑1 (MQ=255)
                                             gTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCa                                                        >  1:1445108/1‑100 (MQ=255)
                                              tCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATACAt                                                       >  1:223340/1‑100 (MQ=255)
                                                agcagTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCAttt                                                     >  1:360503/1‑100 (MQ=255)
                                                agcagTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCAttt                                                     <  2:2256012/100‑1 (MQ=255)
                                                agcagTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCAttt                                                     >  1:1171863/1‑100 (MQ=255)
                                                 gcagTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTc                                                    >  2:2265151/1‑100 (MQ=255)
                                                 gcagTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCAttt                                                     >  1:1956893/1‑99 (MQ=255)
                                                 gcaaTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGACAACGCTTATTACCCAGCTCGATGCAAAAATCCAttt                                                     >  2:1838930/1‑99 (MQ=255)
                                                  cagTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCg                                                   >  2:945087/1‑100 (MQ=255)
                                                    gttgttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATAGTCAACGATTATTACACAGCTCGATGCAAAAATCCATTTc                                                    >  2:1419907/1‑97 (MQ=255)
                                                     ttgttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtg                                                <  2:781511/100‑1 (MQ=255)
                                                     ttgttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtg                                                <  1:30613/100‑1 (MQ=255)
                                                       gttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCtggt                                              >  1:2354511/1‑100 (MQ=255)
                                                           tttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTc                                          <  2:1979817/100‑1 (MQ=255)
                                                           tttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTc                                          <  2:223337/100‑1 (MQ=255)
                                                           tttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTc                                          <  1:1878098/100‑1 (MQ=255)
                                                            ttATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCa                                         <  1:595195/100‑1 (MQ=255)
                                                              aTCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGa                                       >  2:2370067/1‑100 (MQ=255)
                                                               tCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGAt                                      <  2:534203/100‑1 (MQ=255)
                                                                 gCCAATCCACATCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGc                                    >  2:771106/1‑100 (MQ=255)
                                                                   cAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCgg                                  >  1:919041/1‑100 (MQ=255)
                                                                    aaTCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGTggg                                 >  1:1527161/1‑100 (MQ=255)
                                                                     aTCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGa                                <  2:2251430/100‑1 (MQ=255)
                                                                       ccACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATg                              >  2:37426/1‑100 (MQ=255)
                                                                       ccACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATg                              <  1:926489/100‑1 (MQ=255)
                                                                        cacaTCTGTGAAAGAAAGCCTGACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATgg                             >  1:340354/1‑100 (MQ=255)
                                                                          caTCTGTGATAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATTCATTACGCTGGTGGACAGATGCGGCATGGCg                           >  2:2342340/1‑100 (MQ=255)
                                                                          caTCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCg                           >  1:2277123/1‑100 (MQ=255)
                                                                            tCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTg                         <  1:1012602/100‑1 (MQ=255)
                                                                            tCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTg                         <  1:1360268/100‑1 (MQ=255)
                                                                              tgtgAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTggg                       <  2:404901/100‑1 (MQ=255)
                                                                              tgtgAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTggg                       <  1:1090438/100‑1 (MQ=255)
                                                                               gtgAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGa                      >  2:1369620/1‑100 (MQ=255)
                                                                               gtgAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGa                      >  1:1034391/1‑100 (MQ=255)
                                                                                tgAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGAc                     >  2:2072545/1‑100 (MQ=255)
                                                                                 gaaagaaaGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGAcg                    >  2:1745918/1‑100 (MQ=255)
                                                                                 gaaagaaaGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGAcg                    >  2:720783/1‑100 (MQ=255)
                                                                                 gaaagaaaGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGAcg                    >  2:1828094/1‑100 (MQ=255)
                                                                                     gaaagCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGAcgcg                  >  1:1396137/1‑98 (MQ=255)
                                                                                       aagCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGcggcgg              <  2:1548935/100‑1 (MQ=255)
                                                                                        agCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACgcggc                >  1:775348/1‑97 (MQ=255)
                                                                                         gCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCgggg            <  1:1444661/100‑1 (MQ=255)
                                                                                             gACTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCg        >  1:1370252/1‑100 (MQ=255)
                                                                                              aCTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGt       <  1:1050800/100‑1 (MQ=255)
                                                                                               cTGGTGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTc      >  2:2339052/1‑100 (MQ=255)
                                                                                               cTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTc      <  1:357340/100‑1 (MQ=255)
                                                                                                tggtggTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTca     <  1:1421010/100‑1 (MQ=255)
                                                                                                tGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTca     <  2:2314469/100‑1 (MQ=255)
                                                                                                 gtcGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTcac    <  2:1454019/98‑1 (MQ=255)
                                                                                                 ggCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCGGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTcac    >  1:775785/1‑100 (MQ=255)
                                                                                                 ggCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTcac    <  1:848479/100‑1 (MQ=255)
                                                                                                  gCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTcaca   >  1:1005771/1‑100 (MQ=255)
                                                                                                   cGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCacac  >  1:1752373/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
ATGTCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACAC  >  NC_000913/363925‑364123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑