Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,926,039 A→T 15.7% Q231L (CAG→CTG)  queF → 7‑cyano‑7‑deazaguanine reductase (NADPH‑dependent)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,926,0390AT15.7% 262.0 / 41.2 102Q231L (CAG→CTG) queF7‑cyano‑7‑deazaguanine reductase (NADPH‑dependent)
Reads supporting (aligned to +/- strand):  ref base A (44/42);  new base T (9/7);  total (53/49)
Fisher's exact test for biased strand distribution p-value = 7.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-02

Only 100 of 108 total aligned reads displayed.

GGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAA  >  NC_000913/2925940‑2926138
                                                                                                   |                                                                                                   
ggggTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACa                                                                                                     <  1:762057/100‑1 (MQ=255)
ggggTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACa                                                                                                     >  1:970157/1‑100 (MQ=255)
ggggTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACa                                                                                                     >  2:1297865/1‑100 (MQ=255)
   gTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCGGGTCTCATTCCGTCATCACAACGAGTTCCACGAACa                                                                                                     >  2:2002957/1‑97 (MQ=255)
       gCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTg                                                                                              >  2:2145090/1‑100 (MQ=255)
       gCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTg                                                                                              >  1:1678943/1‑100 (MQ=255)
       gCTCCAAATTCAGTATCGTGGACGCCAAATTAACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTg                                                                                              <  2:1933132/100‑1 (MQ=255)
        cTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTgg                                                                                             >  2:2308027/1‑100 (MQ=255)
         tCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGa                                                                                            <  1:2164102/100‑1 (MQ=255)
          ccAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGaa                                                                                           >  2:621088/1‑100 (MQ=255)
           cAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAAc                                                                                          <  1:536400/100‑1 (MQ=255)
            aaaTTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCCTTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAAct                                                                                         <  2:1303438/100‑2 (MQ=255)
            aaaTTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACg                                                                                         <  1:279464/100‑1 (MQ=255)
             aaTTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActc                                                                                        <  1:21851/100‑3 (MQ=255)
             aaTTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGc                                                                                        >  2:1924378/1‑100 (MQ=255)
              aTTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActcg                                                                                       <  1:782016/100‑4 (MQ=38)
              aTTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCa                                                                                       <  2:1141340/100‑1 (MQ=255)
               ttCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActcgt                                                                                      <  1:1008788/100‑5 (MQ=38)
                 cAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActcgttg                                                                                    <  1:1746666/100‑7 (MQ=38)
                 cAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAAAGCATc                                                                                     >  1:1843617/1‑99 (MQ=255)
                  aGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCtt                                                                                   <  1:1077001/100‑1 (MQ=255)
                  aGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCtt                                                                                   <  1:1094129/100‑1 (MQ=255)
                   gTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCTTCATCACAACGAGATCCACGAACAGTGCGTGGAACGCATCttt                                                                                  >  2:429234/1‑100 (MQ=255)
                   gTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCttt                                                                                  >  1:1752144/1‑100 (MQ=255)
                   gTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCttt                                                                                  <  2:1410927/100‑1 (MQ=255)
                        gTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGa                                                                             >  1:1708625/1‑100 (MQ=255)
                             cGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGt                                                                        >  2:566360/1‑100 (MQ=255)
                              gCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGtt                                                                       >  1:1146527/1‑100 (MQ=255)
                               ccAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTa                                                                      >  1:891325/1‑100 (MQ=255)
                               ccAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTa                                                                      >  1:994279/1‑100 (MQ=255)
                               ccAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTa                                                                      >  1:2296034/1‑100 (MQ=255)
                                cAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCATCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTAc                                                                     >  2:1604561/1‑100 (MQ=255)
                                 aaaTTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACg                                                                    <  2:1918851/100‑1 (MQ=255)
                                 aaaTTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGGTCGTGGAACGCATCTTTAATGACCTGTTACg                                                                    >  1:1914647/1‑100 (MQ=255)
                                  aaTTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGc                                                                   <  2:1249734/100‑1 (MQ=255)
                                      gACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCt                                                               >  1:168844/1‑100 (MQ=255)
                                         agagAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgcc                                                            <  1:627870/100‑1 (MQ=255)
                                          gggaagaaCTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgcca                                                           <  2:714738/98‑1 (MQ=255)
                                          gagaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgcca                                                           <  1:891621/100‑1 (MQ=255)
                                          gagaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgcca                                                           <  2:772107/100‑1 (MQ=255)
                                          gagaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgcca                                                           >  2:2052856/1‑100 (MQ=255)
                                           agaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgccag                                                          >  1:443925/1‑100 (MQ=255)
                                            gaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgccagc                                                         >  1:1339647/1‑100 (MQ=255)
                                            gaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgcaagc                                                         >  1:633456/1‑100 (MQ=255)
                                             aaaaaCTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgccagcc                                                        >  2:903939/1‑100 (MQ=255)
                                              aaaaCTGCTGTGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgccagcca                                                       >  1:17850/1‑100 (MQ=255)
                                                  ctgctgCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGaaa                                                   >  2:490482/1‑100 (MQ=255)
                                                       gcGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTTCGTGTGACGCATCTTTAATGACCGGTTACGCTTCTGCCAGCCAGAAAAAtt                                               >  2:1395074/1‑99 (MQ=255)
                                                       gcGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTg                                              <  1:1527095/100‑1 (MQ=255)
                                                       gcGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTg                                              <  1:490485/100‑1 (MQ=255)
                                                         gTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCTAGCCAGAAAAAGTGAg                                            <  2:1235105/100‑1 (MQ=255)
                                                         gTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAg                                            <  1:2212611/100‑1 (MQ=255)
                                                               tGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTa                                      <  1:574023/100‑1 (MQ=255)
                                                               tGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTa                                      >  1:1223548/1‑100 (MQ=255)
                                                                  tttcATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTAc                                     <  1:641692/96‑1 (MQ=255)
                                                                  tctcATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGc                                   >  1:2651/1‑100 (MQ=255)
                                                                  tctcATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGc                                   >  1:1733688/1‑100 (MQ=255)
                                                                   ctcATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCa                                  >  1:2195326/1‑100 (MQ=255)
                                                                     cATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACg                                >  2:1336534/1‑100 (MQ=255)
                                                                        tCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTta                             <  1:1057927/100‑1 (MQ=255)
                                                                        tCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTta                             <  1:2168342/100‑1 (MQ=255)
                                                                        tCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTta                             <  2:462848/100‑1 (MQ=255)
                                                                         ccGTCATAACAACGAGTTCCACGAAGAGTGCGTGGAACGAATCTTTAATGACCTGTTACGCTTATGCAAGACAGAAAAATTGAGCGTTTACGCACGtt                              <  2:2251881/98‑1 (MQ=255)
                                                                          cGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTtata                           >  1:634139/1‑100 (MQ=255)
                                                                            tcatcaCAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATAcc                         <  1:203052/100‑1 (MQ=255)
                                                                            tcatcaCAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATAcc                         <  2:1519023/100‑1 (MQ=255)
                                                                            tcatcaCAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATAcc                         <  1:452961/100‑1 (MQ=255)
                                                                              atcaCAACGAGTTCCACGAACAGTGCGTGCAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCcg                       <  2:1439571/100‑1 (MQ=255)
                                                                              atcaCAACGAGTTCCACGAACAGTGCATAGAACGCAACTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTTAGAGTTTACGCACGTTATAcc                         <  2:1504123/98‑1 (MQ=255)
                                                                                cacaACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCcgtc                     <  2:1992404/100‑1 (MQ=255)
                                                                                cacaACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTGACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCcgtc                     >  2:1067746/1‑100 (MQ=255)
                                                                                  caACGAGTTCCACGAACAGTGCGTGGAACGCATGTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCcgtcgt                   >  2:1442895/1‑100 (MQ=255)
                                                                                   gatgcgTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTg                  >  1:1001026/6‑100 (MQ=38)
                                                                                    ctgcgTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTgg                 >  1:572333/5‑100 (MQ=38)
                                                                                    atgcgTTCCACGCACTGTTTGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTgg                 >  2:1494153/5‑100 (MQ=255)
                                                                                    atgcgTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTgg                 >  1:2307255/5‑100 (MQ=38)
                                                                                    atgcgTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTgg                 >  1:1086251/5‑100 (MQ=38)
                                                                                    atgcgTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTgg                 >  1:960260/5‑100 (MQ=38)
                                                                                    atgcgTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCAAGTTATACCCGTCATgg                 >  2:2018082/5‑100 (MQ=38)
                                                                                     cGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGATTACGCACGTTATACCCGTCGTgg                 >  1:2219747/1‑99 (MQ=255)
                                                                                     cGAGTGCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCTAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGc                <  2:1663018/100‑1 (MQ=255)
                                                                                        gTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGt             >  2:1709909/1‑100 (MQ=255)
                                                                                         ttCCACGAACAGTGCGTGTAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTc            <  1:1395088/100‑1 (MQ=255)
                                                                                         ttCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTc            <  2:922058/100‑1 (MQ=255)
                                                                                         ttCCACGAACAGTGCGTGGAACGCATCTTTAATGAACTGTTACGCTTCTGCCAGCCAGAAAAATTTATCGTTTACTTACGTTATACTGGTCGTGGCg               <  2:526164/97‑1 (MQ=255)
                                                                                           ccACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTg          <  2:1375499/100‑1 (MQ=255)
                                                                                           ccACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTg          <  1:1228804/100‑1 (MQ=255)
                                                                                            cACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTgg         >  2:2290098/1‑100 (MQ=255)
                                                                                            cACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTgg         >  2:1536526/1‑100 (MQ=255)
                                                                                            cACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTgg         >  1:7249/1‑100 (MQ=255)
                                                                                            cACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTgg         >  1:656465/1‑100 (MQ=255)
                                                                                            cACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTgg         <  1:1336548/100‑1 (MQ=255)
                                                                                            cACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCCTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTAGCGGTCTg          >  1:866127/1‑99 (MQ=255)
                                                                                              cGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGAc       <  2:2090110/100‑1 (MQ=255)
                                                                                              cGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGAc       <  2:600430/100‑1 (MQ=255)
                                                                                                aaCAGTGCGTGGAACGCATCTTTAATGACCTTTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACAt     <  1:1782097/100‑1 (MQ=255)
                                                                                                aaCAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACa      <  1:750203/99‑1 (MQ=255)
                                                                                                aaCAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACAt     >  2:773236/1‑100 (MQ=255)
                                                                                                  cAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTa   >  1:1125511/1‑100 (MQ=255)
                                                                                                   aGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTaa  <  2:2403095/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAA  >  NC_000913/2925940‑2926138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: