Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,041,028 G→A 23.0% R15C (CGT→TGT)  sdhE ← flavinator of succinate dehydrogenase; antitoxin of CptAB toxin‑antitoxin pair

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,041,0280GA23.0% 218.4 / 53.2 110R15C (CGT→TGT) sdhEflavinator of succinate dehydrogenase; antitoxin of CptAB toxin‑antitoxin pair
Reads supporting (aligned to +/- strand):  ref base G (36/48);  new base A (10/15);  total (47/63)
Fisher's exact test for biased strand distribution p-value = 8.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

CAGCAGACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCC  >  NC_000913/3040930‑3041125
                                                                                                  |                                                                                                 
cagcagACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCAcgg                                                                                                  >  2:624709/1‑100 (MQ=255)
cagcagACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCAcgg                                                                                                  >  1:1576370/1‑100 (MQ=255)
  gcagACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCAcggcg                                                                                                <  2:1623369/100‑1 (MQ=255)
    agACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACggcggc                                                                                              >  2:662955/1‑100 (MQ=255)
    agACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACagcggc                                                                                              >  1:1639267/1‑100 (MQ=255)
     gACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCa                                                                                             <  1:1207541/100‑1 (MQ=255)
       cGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATg                                                                                           >  2:1052903/1‑100 (MQ=255)
        gAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGc                                                                                          >  2:1566484/1‑100 (MQ=255)
         aaTAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGcc                                                                                         >  1:418918/1‑100 (MQ=255)
            aaaGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCaa                                                                                      <  1:1312067/100‑1 (MQ=255)
             aaGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATAGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCaat                                                                                     <  2:1630766/100‑1 (MQ=255)
              aGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCaatn                                                                                    <  2:2091233/100‑2 (MQ=255)
              aGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCaatg                                                                                    <  2:1078881/100‑1 (MQ=255)
              aGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCaatg                                                                                    <  1:1143961/100‑1 (MQ=255)
               gATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCaatga                                                                                   >  2:34397/1‑100 (MQ=255)
               gATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCaatga                                                                                   >  2:1603983/1‑100 (MQ=255)
               gATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCaatga                                                                                   >  2:1881100/1‑100 (MQ=255)
                 tGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGTCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCaatgaat                                                                                 <  1:1171069/100‑1 (MQ=255)
                  gCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCaatgaatg                                                                                <  1:687914/100‑1 (MQ=255)
                    gTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCTCAATGAATGCg                                                                              >  2:104913/1‑100 (MQ=255)
                    gTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCaatgaat                                                                                 >  2:1147168/1‑97 (MQ=255)
                    gTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCg                                                                              >  1:726442/1‑100 (MQ=255)
                         tCGTAATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCt                                                                          <  2:415853/99‑1 (MQ=255)
                            tCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTttgt                                                                      <  1:1323223/100‑1 (MQ=255)
                            tCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTttgt                                                                      <  1:1608480/100‑1 (MQ=255)
                              tttgcatAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTttgttg                                                                    <  1:2224104/94‑1 (MQ=255)
                              aTCTCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTttgttg                                                                    <  1:211937/100‑1 (MQ=255)
                              aTCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTttgttg                                                                    <  2:1727151/100‑1 (MQ=255)
                               tCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTtgttgt                                                                   >  1:2349284/1‑100 (MQ=255)
                               tCGCTTAAGCTGTCGTACTAATGTTGGAAAAACGGCAGGATTGAAATATCGAGTTCGCGCATACCACAGCGACCTGCCCAATGAAATCGGGCtt                                                                         >  2:583549/1‑94 (MQ=255)
                                cGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTgttgtt                                                                  >  2:2389502/1‑100 (MQ=255)
                                cGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTgttgtt                                                                  <  2:1335275/100‑1 (MQ=255)
                                cGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTgttgtt                                                                  <  1:780782/100‑1 (MQ=255)
                                cGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTgttgtt                                                                  >  1:457840/1‑100 (MQ=255)
                                 gCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTa                                                                 >  1:935857/1‑100 (MQ=255)
                                 gCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTa                                                                 <  2:1831548/100‑1 (MQ=255)
                                    tAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATg                                                              >  2:49695/1‑100 (MQ=255)
                                    tAAGCTGTCGTACCCATGTTCGAAAACCGGCATGATTGAACTATCGAGTTCGCGCATAACACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAAtc                                                              <  2:1428189/100‑2 (MQ=255)
                                      atcTGTCGTACTCATGTTCGAAAAACGGCATGATTGAGATATCGAGTGCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTc                                                            <  2:1448797/98‑1 (MQ=255)
                                      aGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTc                                                            <  2:362139/100‑1 (MQ=255)
                                      aGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTc                                                            >  2:737115/1‑100 (MQ=255)
                                        cTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGGATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCa                                                          >  2:2023168/1‑100 (MQ=255)
                                        cTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGTCCAATGAATGCGGGCTTTGTTGTTAATGTCCa                                                          <  1:2280039/100‑1 (MQ=255)
                                        cTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCa                                                          >  2:79409/1‑100 (MQ=255)
                                          gTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCAtc                                                        >  1:2324559/1‑100 (MQ=255)
                                            cGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCAtctt                                                      <  1:830478/100‑1 (MQ=255)
                                                cTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCttcttc                                                  <  2:918277/100‑1 (MQ=255)
                                                cTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCttcttc                                                  >  1:184892/1‑100 (MQ=255)
                                                  cATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCt                                                >  2:2376913/1‑100 (MQ=255)
                                                  cATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCt                                                >  1:1206304/1‑100 (MQ=255)
                                                  cATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCt                                                >  1:1072189/1‑100 (MQ=255)
                                                  cATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCt                                                <  1:366191/100‑1 (MQ=255)
                                                   aTGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTg                                               <  2:2176917/100‑1 (MQ=255)
                                                   aTGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTg                                               <  1:1496706/100‑1 (MQ=255)
                                                    tGTTCGAAAAACGGCATGATTGAAATATCGAGGTCGCGCATACCACGGCGGCATGCCCCATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGt                                              >  1:1298165/1‑100 (MQ=255)
                                                     ggtCGAAAAACGGAATGATTGAAATATCGAGATCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtc                                             >  1:1613479/3‑100 (MQ=255)
                                                     gTTCGAAAAACGGCATGATTGAAATATCGAGTTTGCGCATACCACGGCGGCATCCCCAATGAATGCGGACTTTGTTGTTAATGTCCATCTTCTTTCTGtc                                             >  2:14340/1‑100 (MQ=255)
                                                     gTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtc                                             <  2:785127/100‑1 (MQ=255)
                                                     gTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtc                                             <  1:737123/100‑1 (MQ=255)
                                                      ttCTAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCTTGCTTTGTTGTTAATGTCCATCTTCTTCCTGtct                                            <  2:1839543/100‑1 (MQ=255)
                                                       tCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtctc                                           >  2:1431143/1‑100 (MQ=255)
                                                       tCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGtctc                                           <  1:1800618/100‑1 (MQ=255)
                                                        cGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCa                                          >  2:496199/1‑100 (MQ=255)
                                                         gAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCAc                                         <  2:285296/100‑1 (MQ=255)
                                                          aaaaaCGGCATGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACg                                        <  2:1622984/100‑1 (MQ=255)
                                                           aaaaCGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGa                                       <  2:1852880/100‑1 (MQ=255)
                                                              aCGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGaaaa                                    <  1:1872442/100‑1 (MQ=255)
                                                               cGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAAt                                   <  2:1410008/100‑1 (MQ=255)
                                                                 gCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATc                                  >  2:2109628/1‑99 (MQ=255)
                                                                  cATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCa                                <  1:342238/100‑1 (MQ=255)
                                                                  cATGATTGAAATATCGAGTTCGCGCATACCACCGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCa                                >  1:2279042/1‑100 (MQ=255)
                                                                   ttGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAg                               <  1:131133/99‑1 (MQ=255)
                                                                   aTGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCa                                <  1:1528583/99‑1 (MQ=255)
                                                                   aTGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAg                               <  2:1367812/100‑1 (MQ=255)
                                                                   aTGATTGAAATATCGAGTTCGCGCATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAg                               <  2:2198325/100‑1 (MQ=255)
                                                                    tGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAga                              >  2:131130/1‑99 (MQ=255)
                                                                    tGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGt                              <  2:502753/100‑1 (MQ=255)
                                                                     gATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTa                             >  1:541111/1‑100 (MQ=255)
                                                                      aTTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTAc                            <  1:995249/100‑1 (MQ=255)
                                                                      aTTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTAc                            <  2:620147/100‑1 (MQ=255)
                                                                      aTTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTAc                            <  2:620240/100‑1 (MQ=255)
                                                                       ttGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTAcc                           >  1:2172519/1‑100 (MQ=255)
                                                                         gAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCg                         >  1:2104549/1‑100 (MQ=255)
                                                                          aaaTATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCgg                        <  1:1220420/100‑1 (MQ=255)
                                                                           aaTATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGc                       <  1:1279108/100‑1 (MQ=255)
                                                                              atCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTAt                    >  2:1987359/1‑100 (MQ=255)
                                                                               tCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTAtt                   >  1:1762120/1‑100 (MQ=255)
                                                                                 gAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGt                 <  1:1566503/100‑1 (MQ=255)
                                                                                 gAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGt                 <  1:904210/100‑1 (MQ=255)
                                                                                     tCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACg             <  1:353369/100‑1 (MQ=255)
                                                                                     tCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACg             <  1:600477/100‑1 (MQ=255)
                                                                                      cgcgCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACgt            >  1:1132424/1‑100 (MQ=255)
                                                                                        cgcATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACgtgt          <  1:556002/100‑1 (MQ=255)
                                                                                          cATACCACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGttt        >  2:2057495/1‑100 (MQ=255)
                                                                                              ccACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGttttt      >  1:1415735/1‑98 (MQ=255)
                                                                                              ccACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCg    <  2:1894240/100‑1 (MQ=255)
                                                                                              ccACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCg    <  2:1291290/100‑1 (MQ=255)
                                                                                              ccACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCg    <  2:1222867/100‑1 (MQ=255)
                                                                                              ccACAGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCg    >  2:63149/1‑100 (MQ=255)
                                                                                                aCGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGcc  <  1:662961/100‑1 (MQ=255)
                                                                                                  |                                                                                                 
CAGCAGACGAATAAAGATGCGTTTTTCGTCATCGCTTAAGCTGTCGTACTCATGTTCGAAAAACGGCATGATTGAAATATCGAGTTCGCGCATACCACGGCGGCATGCCCAATGAATGCGGGCTTTGTTGTTAATGTCCATCTTCTTCCTGTCTCACGAAAATCCAGTACCCGGCTATTGTAACGTGTTTTTCGCC  >  NC_000913/3040930‑3041125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: