Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,057,140 A→G 16.2% intergenic (‑191/+38) ibsC ← / ← serA toxic membrane protein/D‑3‑phosphoglycerate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,057,1400AG16.2% 250.2 / 38.4 113intergenic (‑191/+38)ibsC/serAtoxic membrane protein/D‑3‑phosphoglycerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (48/46);  new base G (9/9);  total (57/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

Only 100 of 114 total aligned reads displayed.

CGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTT  >  NC_000913/3057044‑3057237
                                                                                                |                                                                                                 
 gCAGTGATCTAACTGTGATTTATTTTCGAGAGTAGACACGCAAAGTAAAATGCGCAGCGCGGCAACGGTGTTGAGAAGGTATAAAAAAACGGGCAAGTc                                                                                                <  2:1990493/99‑1 (MQ=255)
  cATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAg                                                                                              <  1:1529258/100‑1 (MQ=255)
  cATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGAGTGGAGAAGGGATAAAAAAACGGGCAAGTCAg                                                                                              >  1:81020/1‑100 (MQ=255)
   aTTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTATAATGCGCAACGCTGCAACGGTGTGGATAAGTGATAAAAAAACGGGCAAGTCAg                                                                                              <  2:2187181/99‑1 (MQ=255)
   aTTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTc                                                                                                <  1:193801/97‑1 (MQ=255)
      gATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGAc                                                                                          >  2:259087/1‑100 (MQ=255)
       aTCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGAcc                                                                                         <  1:218470/100‑1 (MQ=255)
       aTCTGACTTTGATTTATTTTCTGGAGCAGACGCGCAAAGTAGAATGCGCAACGCGGCAACGGTTTGGAGAAGGTATAAAAAAACGGGCAAGTCAGTGAcc                                                                                         <  2:2138642/100‑1 (MQ=255)
        tcagACTTTTATTTATTTTCTTTAACACACACTAAAATTAGAATGCGCAACGCGGCAACGGTGTTAAAAAAAAAAAAAAAAACGGGCAAGTCAg                                                                                              <  2:1997035/91‑1 (MQ=255)
         cTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCt                                                                                        >  2:368381/1‑99 (MQ=255)
          tGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGc                                                                                      >  1:937910/1‑100 (MQ=255)
           gACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGcc                                                                                     <  1:1199163/100‑1 (MQ=255)
            aCTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGccc                                                                                    >  1:1741786/1‑100 (MQ=255)
             cTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCg                                                                                   >  2:2049039/1‑100 (MQ=255)
             cTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCg                                                                                   >  2:2395720/1‑100 (MQ=255)
              tttGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGt                                                                                  <  1:1805107/100‑1 (MQ=39)
              tttGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGt                                                                                  <  1:2036454/100‑1 (MQ=255)
              tttGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCACAACGCGGCACCGGTGTGGAGAAGGGATAAAATATCTGGCATGTCAGTGAGCTGCCCg                                                                                   <  2:533758/99‑1 (MQ=255)
                tGATTTATTTCCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGACAATAAAACGGGCAAGTCAGTGACCTGCCCGTTg                                                                                <  2:1572104/100‑1 (MQ=255)
                 gATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGtttt                                                                               <  2:1542440/100‑3 (MQ=38)
                 gATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGcccagatc                                                                               >  1:1795120/1‑95 (MQ=255)
                 gATTTATTTGCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGa                                                                               <  2:2340637/100‑1 (MQ=255)
                 gATTTATGTGGTGGGGCAGAGTGGGAAAGTAGGATGCGGAAGGCGGCAACGGTGTGGAGAAGGGATAAGATAACGGGCAGGTCACTGACGTGCCCGtttt                                                                               <  2:359319/100‑3 (MQ=16)
                  atttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGttttt                                                                              <  2:626297/100‑4 (MQ=38)
                   tttatttTCTGGAGCAGACTCGCAAAGAAGAATGCGCAACGCGGCAACGGTATGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAtt                                                                             >  2:751768/1‑100 (MQ=255)
                   tttatttTCTGGAGCAGACTCGCAAAATAGAATGCGCTACGCGGGAACGGTGTGGAGAAGGGATAAAAAAACGGGCAATTCTGTGACCTGCCCGTTGAtt                                                                             <  2:1069122/100‑1 (MQ=255)
                    ttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGttttttt                                                                            <  1:1135638/100‑6 (MQ=37)
                    ttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAttt                                                                            <  2:1858549/100‑1 (MQ=255)
                     tatttTCTGGAGCAGACTGGCAAAGTAGAATACGCAACGAAGGAAAGGAGAGGAGAAGGGATAAAAAAACGGGCAAGGCAATGATGTGCCCGTTGAtatt                                                                           >  2:1545688/1‑100 (MQ=255)
                      atttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTc                                                                          >  2:78421/1‑100 (MQ=255)
                      atttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTc                                                                          <  1:720828/100‑1 (MQ=255)
                       ttttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGtttttttatc                                                                         <  2:1907253/100‑9 (MQ=37)
                       ttttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCa                                                                         <  2:2378895/100‑1 (MQ=255)
                        tttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCag                                                                        >  1:2380115/1‑100 (MQ=255)
                         ttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGtttttttatccc                                                                       <  1:204113/100‑11 (MQ=25)
                         ttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTTACCTGCCCGTTGATTTTCaga                                                                       <  2:953054/100‑1 (MQ=255)
                          tCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCagag                                                                      >  2:346454/1‑100 (MQ=255)
                          tCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCagag                                                                      <  1:1711863/100‑1 (MQ=255)
                                 cAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGa                                                               <  1:482060/100‑1 (MQ=255)
                                 cAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGa                                                               <  2:741191/100‑1 (MQ=255)
                                  aGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGaa                                                              >  2:2271748/1‑100 (MQ=255)
                                    aCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAAtt                                                            <  1:547360/100‑1 (MQ=255)
                                    aCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAAtt                                                            <  1:113600/100‑1 (MQ=255)
                                      tCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAg                                                          <  2:203305/100‑1 (MQ=255)
                                        gCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTa                                                        <  1:427813/100‑1 (MQ=255)
                                            aGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcagc                                                    <  2:936727/100‑1 (MQ=255)
                                               aGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGTAATTAGTACAGCAGa                                                 >  1:2363114/1‑100 (MQ=255)
                                               aGAATGCGCAACGCGGCAACGGTGTGGAGAAAGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGa                                                 >  2:2062298/1‑100 (MQ=255)
                                                   tGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACggg                                             <  1:2324695/100‑1 (MQ=255)
                                                     cgcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGgcg                                           >  2:1765570/1‑100 (MQ=255)
                                                     cgcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGgcg                                           >  1:1438653/1‑100 (MQ=255)
                                                     cgcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGgcg                                           >  1:1000463/1‑100 (MQ=255)
                                                      gcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGTGGAATTAGTACAGCAGACGGgcg                                           <  2:612303/99‑1 (MQ=255)
                                                       cAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGcgcg                                         >  2:1130515/1‑100 (MQ=255)
                                                            cgGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATgg                                    >  2:519256/1‑100 (MQ=255)
                                                               cAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCGGCCCGGTGAATTTCAGAGAAGGGGAAATAGTACAGCAGACGAgcg                                           >  2:895885/1‑90 (MQ=255)
                                                                 aCGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTAccc                               <  1:1582300/100‑1 (MQ=255)
                                                                   ggTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGCCGGGCGCGAATGGTACCCg                              >  2:1329245/1‑99 (MQ=255)
                                                                   ggTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCgg                             <  1:283419/100‑1 (MQ=255)
                                                                    gtgtGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGAGAATAAGTACAGCAGACGGGCGCGAATGGTACCCGGa                            >  2:2287250/1‑100 (MQ=255)
                                                                      gtgGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAAt                          <  1:2130569/100‑1 (MQ=255)
                                                                       tgaagaAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAAt                          <  1:699009/96‑1 (MQ=255)
                                                                        ggAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAg                        >  1:2187584/1‑100 (MQ=255)
                                                                        ggAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAg                        >  1:1147393/1‑100 (MQ=255)
                                                                         gagaAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGc                       >  1:89111/1‑100 (MQ=255)
                                                                          agaAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAg                        <  1:44772/98‑1 (MQ=255)
                                                                           gaAGGGATAAAAAAACGAGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCtt                     >  2:1834440/1‑100 (MQ=255)
                                                                             tctgATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGTGCGAATGGTACCCGGAATAGCTTTc                   <  2:443075/97‑1 (MQ=255)
                                                                             aaaaaaaaaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTc                   <  2:1032836/94‑1 (MQ=255)
                                                                              gggATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCa                  >  2:1863622/1‑100 (MQ=255)
                                                                              gggATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCa                  <  1:2328681/100‑1 (MQ=255)
                                                                               ggATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCAt                 >  2:121632/1‑100 (MQ=255)
                                                                                gATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCaga                >  1:443078/1‑98 (MQ=255)
                                                                                   aaaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGcc             >  1:1010756/1‑100 (MQ=255)
                                                                                   aaaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGcc             >  1:1661785/1‑100 (MQ=255)
                                                                                   aaaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGcc             >  1:2112918/1‑100 (MQ=255)
                                                                                    aatcaaCCGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCt            >  1:1252775/5‑100 (MQ=38)
                                                                                    aaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCt            >  2:2017686/1‑100 (MQ=255)
                                                                                    aaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAAGGGAATTAGTACAGGAGACGGGTGCGAATGGTACCTGGAATAGCTTTCATTGcc             >  2:2183323/1‑99 (MQ=255)
                                                                                    aaaaaaCGGGCAAGTCAGTGACCAGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCt            >  2:646905/1‑100 (MQ=255)
                                                                                     aaaaaCGGGCAAGTCAGTGACCTGCCGGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTg           >  1:1454456/1‑100 (MQ=255)
                                                                                     aaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTg           <  1:2395748/100‑1 (MQ=255)
                                                                                     aaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTg           <  1:1548901/100‑1 (MQ=255)
                                                                                      aaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGc          <  2:2184504/100‑1 (MQ=255)
                                                                                      aaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACACGGAATAGCTTTCATTGCCTGc          >  2:2315779/1‑100 (MQ=255)
                                                                                       caaCGGGCAGGTCACTGACTTGACCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGATTTCATTACGTGCa         >  2:394399/2‑100 (MQ=255)
                                                                                        aaCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAg        >  1:2173454/1‑100 (MQ=39)
                                                                                        aaCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAg        >  1:224496/1‑100 (MQ=39)
                                                                                        aaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCa         <  1:78423/99‑1 (MQ=255)
                                                                                        aaCGGGCAAGTCAGTGACCTGCCCGTTGAATTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGTAATAGCTTCCATTGCCTGCa         <  2:1165831/99‑1 (MQ=255)
                                                                                         aCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgc       >  1:1192012/1‑100 (MQ=39)
                                                                                         aCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgc       >  2:929167/1‑100 (MQ=39)
                                                                                          cGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgcg      >  2:54916/1‑100 (MQ=255)
                                                                                          cGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgcg      >  1:1448245/1‑100 (MQ=255)
                                                                                          cGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCa         <  2:2398078/97‑1 (MQ=255)
                                                                                           gggCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgcgc     >  1:2101567/1‑100 (MQ=255)
                                                                                           gggCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgcgc     >  1:293586/1‑100 (MQ=255)
                                                                                             gCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCtt   >  2:258327/1‑100 (MQ=255)
                                                                                             gCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCtt   >  2:918994/1‑100 (MQ=255)
                                                                                               aaGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCttt  <  1:860575/99‑1 (MQ=255)
                                                                                                |                                                                                                 
CGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTT  >  NC_000913/3057044‑3057237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: