Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,057,145 G→C 16.4% intergenic (‑196/+33) ibsC ← / ← serA toxic membrane protein/D‑3‑phosphoglycerate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,057,1450GC16.4% 297.4 / 49.4 111intergenic (‑196/+33)ibsC/serAtoxic membrane protein/D‑3‑phosphoglycerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (49/43);  new base C (9/9);  total (59/52)
Fisher's exact test for biased strand distribution p-value = 8.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

Only 100 of 115 total aligned reads displayed.

CATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGG  >  NC_000913/3057046‑3057242
                                                                                                   |                                                                                                 
cATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAg                                                                                                   <  1:1529258/100‑1 (MQ=255)
cATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGAGTGGAGAAGGGATAAAAAAACGGGCAAGTCAg                                                                                                   >  1:81020/1‑100 (MQ=255)
 aTTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTATAATGCGCAACGCTGCAACGGTGTGGATAAGTGATAAAAAAACGGGCAAGTCAg                                                                                                   <  2:2187181/99‑1 (MQ=255)
     aTCTGACTTTGATTTATTTTCTGGAGCAGACGCGCAAAGTAGAATGCGCAACGCGGCAACGGTTTGGAGAAGGTATAAAAAAACGGGCAAGTCAGTGAcc                                                                                              <  2:2138642/100‑1 (MQ=255)
     aTCAGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGAcc                                                                                              <  2:2195479/100‑1 (MQ=255)
      tcagACTTTTATTTATTTTCTTTAACACACACTAAAATTAGAATGCGCAACGCGGCAACGGTGTTAAAAAAAAAAAAAAAAACGGGCAAGTCAg                                                                                                   <  2:1997035/91‑1 (MQ=255)
       cTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCt                                                                                             >  2:368381/1‑99 (MQ=255)
       cTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTg                                                                                            >  2:1102948/1‑100 (MQ=255)
        tGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGc                                                                                           >  1:937910/1‑100 (MQ=255)
         gACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGcc                                                                                          <  1:1199163/100‑1 (MQ=255)
          gatctGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGccc                                                                                         <  2:1795099/96‑1 (MQ=255)
          aCTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGccc                                                                                         >  1:1741786/1‑100 (MQ=255)
           cTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCg                                                                                        >  2:2395720/1‑100 (MQ=255)
           cTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCg                                                                                        >  2:2049039/1‑100 (MQ=255)
            tttGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGt                                                                                       <  1:2036454/100‑1 (MQ=255)
              tGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGttt                                                                                     <  2:2031498/100‑2 (MQ=39)
              tGATTTATTTCCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGACAATAAAACGGGCAAGTCAGTGACCTGCCCGTTg                                                                                     <  2:1572104/100‑1 (MQ=255)
               gATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGtttt                                                                                    <  2:243486/100‑3 (MQ=38)
               gATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGtttt                                                                                    <  2:1542440/100‑3 (MQ=38)
               gATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGcccagatc                                                                                    >  1:1795120/1‑95 (MQ=255)
               gATTTATTTGCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGa                                                                                    <  2:2340637/100‑1 (MQ=255)
               gATTTATGTGGTGGGGCAGAGTGGGAAAGTAGGATGCGGAAGGCGGCAACGGTGTGGAGAAGGGATAAGATAACGGGCAGGTCACTGACGTGCCCGtttt                                                                                    <  2:359319/100‑3 (MQ=16)
                atttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGttttt                                                                                   <  2:626297/100‑4 (MQ=38)
                 tttatttTCTGGAGCAGACTCGCAAAATAGAATGCGCTACGCGGGAACGGTGTGGAGAAGGGATAAAAAAACGGGCAATTCTGTGACCTGCCCGTTGAtt                                                                                  <  2:1069122/100‑1 (MQ=255)
                  ttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGttttttt                                                                                 <  1:1135638/100‑6 (MQ=37)
                   tatttTCTGGAGCAGACTGGCAAAGTAGAATACGCAACGAAGGAAAGGAGAGGAGAAGGGATAAAAAAACGGGCAAGGCAATGATGTGCCCGTTGAtatt                                                                                >  2:1545688/1‑100 (MQ=255)
                    atttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTc                                                                               <  1:720828/100‑1 (MQ=255)
                    atttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTc                                                                               >  2:78421/1‑100 (MQ=255)
                     ttttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGtttttttatc                                                                              <  2:1907253/100‑9 (MQ=37)
                     ttttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCa                                                                              <  2:2378895/100‑1 (MQ=255)
                      tttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCag                                                                             >  1:2380115/1‑100 (MQ=255)
                      cttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCag                                                                             <  1:346457/99‑1 (MQ=255)
                       ttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGtttttttatccc                                                                            <  1:204113/100‑11 (MQ=25)
                       ttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTTACCTGCCCGTTGATTTTCaga                                                                            <  2:953054/100‑1 (MQ=255)
                        tCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCagag                                                                           >  2:346454/1‑100 (MQ=255)
                        tCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCagag                                                                           <  1:1711863/100‑1 (MQ=255)
                         cTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAgaga                                                                          >  1:1371641/1‑100 (MQ=255)
                               cAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGa                                                                    <  2:741191/100‑1 (MQ=255)
                                aGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGaa                                                                   >  2:2271748/1‑100 (MQ=255)
                                  aCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAAtt                                                                 <  1:547360/100‑1 (MQ=255)
                                  aCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAAtt                                                                 <  1:113600/100‑1 (MQ=255)
                                   cTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTa                                                                >  1:168613/1‑100 (MQ=255)
                                    tCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAg                                                               <  2:203305/100‑1 (MQ=255)
                                      gCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTa                                                             <  1:427813/100‑1 (MQ=255)
                                          aGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcagc                                                         <  2:936727/100‑1 (MQ=255)
                                             aGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGTAATTAGTACAGCAGa                                                      >  1:2363114/1‑100 (MQ=255)
                                                 tGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACggg                                                  <  1:2324695/100‑1 (MQ=255)
                                                   cgcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGgcg                                                >  1:1000463/1‑100 (MQ=255)
                                                   cgcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGgcg                                                >  1:1438653/1‑100 (MQ=255)
                                                   cgcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGgcg                                                >  2:1765570/1‑100 (MQ=255)
                                                    gcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGTGGAATTAGTACAGCAGACGGgcg                                                <  2:612303/99‑1 (MQ=255)
                                                     cAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGcgcg                                              >  2:1130515/1‑100 (MQ=255)
                                                     cAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGAGGAATTAGTACAGCAGACGGGcgcg                                              >  1:745950/1‑100 (MQ=255)
                                                          cgGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATgg                                         <  2:742379/100‑1 (MQ=255)
                                                          cgGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATgg                                         >  2:519256/1‑100 (MQ=255)
                                                             cAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCGGCCCGGTGAATTTCAGAGAAGGGGAAATAGTACAGCAGACGAgcg                                                >  2:895885/1‑90 (MQ=255)
                                                                 ggTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCgg                                  <  1:283419/100‑1 (MQ=255)
                                                                  gtgtGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGAGAATAAGTACAGCAGACGGGCGCGAATGGTACCCGGa                                 >  2:2287250/1‑100 (MQ=255)
                                                                    gtgGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAAt                               <  1:2130569/100‑1 (MQ=255)
                                                                     tgaagaAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAAt                               <  1:699009/96‑1 (MQ=255)
                                                                      ggAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAg                             >  1:1147393/1‑100 (MQ=255)
                                                                      ggAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAg                             >  1:2187584/1‑100 (MQ=255)
                                                                        agaAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAg                             <  1:44772/98‑1 (MQ=255)
                                                                         gaAGGGATAAAAAAACGAGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCtt                          >  2:1834440/1‑100 (MQ=255)
                                                                           tctgATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGTGCGAATGGTACCCGGAATAGCTTTc                        <  2:443075/97‑1 (MQ=255)
                                                                           aaaaaaaaaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTc                        <  2:1032836/94‑1 (MQ=255)
                                                                            gggATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCa                       >  2:1863622/1‑100 (MQ=255)
                                                                            gggATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCa                       <  1:2328681/100‑1 (MQ=255)
                                                                             ggATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCAt                      >  2:121632/1‑100 (MQ=255)
                                                                              gATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCaga                     >  1:443078/1‑98 (MQ=255)
                                                                              gATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCAtt                     <  1:2049061/100‑1 (MQ=255)
                                                                                 aaaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGcc                  >  1:1010756/1‑100 (MQ=255)
                                                                                 aaaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGcc                  >  2:490503/1‑100 (MQ=255)
                                                                                 aaaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGcc                  >  1:1661785/1‑100 (MQ=255)
                                                                                 aaaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGcc                  >  1:2112918/1‑100 (MQ=255)
                                                                                  aaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCt                 >  2:2017686/1‑100 (MQ=255)
                                                                                  aaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAAGGGAATTAGTACAGGAGACGGGTGCGAATGGTACCTGGAATAGCTTTCATTGcc                  >  2:2183323/1‑99 (MQ=255)
                                                                                  aaaaaaCGGGCAAGTCAGTGACCAGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCt                 >  2:646905/1‑100 (MQ=255)
                                                                                   aaaaaCGGGCAAGTCAGTGACCTGCCGGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTg                >  1:1454456/1‑100 (MQ=255)
                                                                                   aaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTg                <  1:1548901/100‑1 (MQ=255)
                                                                                   aaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTg                <  1:2395748/100‑1 (MQ=255)
                                                                                    aaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGc               <  2:2184504/100‑1 (MQ=255)
                                                                                     caaCGGGCAGGTCACTGACTTGACCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGATTTCATTACGTGCa              >  2:394399/2‑100 (MQ=255)
                                                                                      aaCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAg             >  1:224496/1‑100 (MQ=39)
                                                                                      aaCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAg             >  1:2173454/1‑100 (MQ=39)
                                                                                      aaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCa              <  1:78423/99‑1 (MQ=255)
                                                                                      aaCGGGCAAGTCAGTGACCTGCCCGTTGAATTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGTAATAGCTTCCATTGCCTGCa              <  2:1165831/99‑1 (MQ=255)
                                                                                       aCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgc            >  2:929167/1‑100 (MQ=39)
                                                                                       aCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgc            >  1:1192012/1‑100 (MQ=39)
                                                                                        cGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgcg           >  2:54916/1‑100 (MQ=255)
                                                                                        cGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgcg           >  1:1448245/1‑100 (MQ=255)
                                                                                        cGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCa              <  2:2398078/97‑1 (MQ=255)
                                                                                         gggCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgcgc          >  1:2101567/1‑100 (MQ=255)
                                                                                         gggCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgcgc          >  1:293586/1‑100 (MQ=255)
                                                                                           gCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCtt        >  2:918994/1‑100 (MQ=255)
                                                                                           gCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCtt        >  2:258327/1‑100 (MQ=255)
                                                                                             aaGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCttt       <  1:860575/99‑1 (MQ=255)
                                                                                                tCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCg   >  1:1446817/1‑100 (MQ=255)
                                                                                                 cAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCgg  >  2:558450/1‑100 (MQ=255)
                                                                                                 cAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCgg  <  1:1663018/100‑1 (MQ=255)
                                                                                                   |                                                                                                 
CATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGG  >  NC_000913/3057046‑3057242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: