Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,128,863 A→T 16.7% I198F (ATC→TTC)  glcC → glycolate‑inducible glc operon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,128,8630AT16.7% 216.7 / 35.2 90I198F (ATC→TTC) glcCglycolate‑inducible glc operon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base A (36/39);  new base T (9/6);  total (45/45)
Fisher's exact test for biased strand distribution p-value = 5.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01

Only 100 of 101 total aligned reads displayed.

TGCTGGTGTTTACGCTGCAATCATTGACCGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAG  >  NC_000913/3128765‑3128961
                                                                                                   |                                                                                                  
tGCTGGTGTTTACGCTGCAATCATTGACCGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAga                                                                                                    >  1:1888260/1‑99 (MQ=255)
 gCTGGTGTTTACGCTGCAATCATTGACCGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAgttc                                                                                                  <  2:1733756/100‑1 (MQ=255)
 gCTGGTGTTTACGCTGCAATCATTGACCGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAgatc                                                                                                  >  2:1119233/1‑100 (MQ=255)
 gCTGGTGTTTACGCTGCAATCATTGACCGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAgatc                                                                                                  >  2:1820846/1‑100 (MQ=255)
  cTGGTGTTTACGCTGCAATCATTGACCGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAgatcg                                                                                                 >  2:1228520/1‑100 (MQ=255)
  cTGGTGTTTACGCTGCAATCATTGACCGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAgatcg                                                                                                 >  1:2394245/1‑100 (MQ=255)
    ggTGTTTACGCTGCAATCATTGACCGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAgatcgat                                                                                               >  2:2062000/1‑100 (MQ=255)
     gtgtTTACGCTGCAATCATTGACCGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAgatcgatc                                                                                              <  2:1858354/100‑1 (MQ=255)
      tgtTTACGCTGCAATCATTGACCGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGatcgatcg                                                                                             >  1:2091216/1‑100 (MQ=255)
      tgtTTACGCTGCAATCATTGACAGATCTGATGGATAGTTCAGTTTTTACGAACAGAAATAATCTATACCAGCCACAACAGCAAAAAAAGCAgagctaga                                                                                              >  2:2062563/1‑93 (MQ=255)
         ttACGCTGCAATCATTGACCGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCa                                                                                          >  2:1772556/1‑100 (MQ=255)
            cGCTGCAATCATTGACCGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCa                                                                                       >  2:237201/1‑100 (MQ=255)
            cGCTGCAATCATTGACCGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCa                                                                                       <  1:2253601/100‑1 (MQ=255)
               tGCAATCATTGACCGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGTTCGATCGCCAGCATgc                                                                                    >  1:1021879/1‑100 (MQ=255)
               tGCAATCATTGACCGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCAAAGCAAAAAAAGCAGATCGATCGCCAGc                                                                                        <  2:449997/96‑1 (MQ=255)
                gCAATCCTTGACCGATCTGATATTTAATTCAGTGTTTTCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAACCAGAGCGATCGCCAGCATgcg                                                                                   >  2:597838/1‑100 (MQ=255)
                 cAATCATTGACCGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATgcgc                                                                                  >  1:817031/1‑100 (MQ=255)
                 cAATCATTGACCGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATgcgc                                                                                  >  1:1332217/1‑100 (MQ=255)
                   aTCATTGACCGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGTTCGATCGCCAGCATGCGCgg                                                                                >  1:690321/1‑100 (MQ=255)
                   aTCATTAACCGGCCTGATGTTCAATAAGATGTCTTCCATTGTAAATAATCTCTATCAGAAATCAAAACAAAAAAAGCAGATCGATCGCCAGCATgcg                                                                                   <  2:2351238/97‑1 (MQ=255)
                     cATTGACCGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGAt                                                                              >  2:351197/1‑100 (MQ=255)
                     cATTGACCGATCTGATGTTTAATTCAGTGTTTGCCAGAGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGAt                                                                              <  2:1051419/100‑1 (MQ=255)
                         gACCGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGTTCGATCGCCAGCATGCGCGGATCTAc                                                                          >  2:630667/1‑100 (MQ=255)
                          aacGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATATCTATCATCGACCACAGCAAAAAAAGCAGATCGTTCTCCAGTATGCGCGGATCTACa                                                                         <  2:1985790/98‑1 (MQ=255)
                          aCCGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACa                                                                         <  2:31902/100‑1 (MQ=255)
                            cGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGTTCGATCGCCAGCATGCGCGGATCTACAAc                                                                       <  1:1348579/100‑1 (MQ=255)
                            cGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGTTCGATCGCCAGCATGCGCGGATCTACAAc                                                                       <  1:1772655/100‑1 (MQ=255)
                             gATCTGATTTTTAATTCAGTATTTGCAAGCGTAAATAATATCTAAAATCGACCACAGCAAAAAAAGAAGATGTATAGAAAGCAAGAGAGGATCTACa                                                                         >  2:51829/1‑97 (MQ=255)
                             gATCTGATGTTTAATTCAGTGTTTGCCAGTGTAAATAATCTCTAGCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAAcg                                                                      <  2:1405693/100‑1 (MQ=255)
                             gATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACAAAAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAAcg                                                                      <  2:1591047/100‑1 (MQ=255)
                              aTCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAAcgc                                                                     <  1:772017/100‑1 (MQ=255)
                                  gATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGgtg                                                                 <  2:1417595/100‑1 (MQ=255)
                                   aTGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGgtgt                                                                <  1:1451877/100‑1 (MQ=255)
                                    tGTTTAATTCAGTTTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGtt                                                               <  2:782723/100‑1 (MQ=255)
                                    tGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACCGCAAAAAAAGCAGTTCGATCGCCAGCATGCGCGGATCTACAACGCGgtg                                                                 >  2:490987/1‑98 (MQ=255)
                                    tGTTTAATTAAGTTTTTGCCAGCGTAAATAATCTCTACCAATGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGgtgt                                                                <  1:385186/99‑1 (MQ=255)
                                     gTTTAATTCAGTGTTTGTCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTg                                                              >  1:465696/1‑100 (MQ=255)
                                     gTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTg                                                              >  2:671297/1‑100 (MQ=255)
                                      tttAATTCAGTTTTTGCCAGCGTAAATAATCTCTACCATCAACCACAGAAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGc                                                             <  2:2129693/100‑1 (MQ=255)
                                         aaTTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGc                                                          >  2:512676/1‑100 (MQ=255)
                                          aTTCAGTGTTTTCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCg                                                         <  2:1717634/100‑1 (MQ=255)
                                          aTTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCg                                                         >  1:1334363/1‑100 (MQ=255)
                                          aTTCAGTGTTTGCCAGCATAAAAAAATTCTACCATCGACCACAGCAAAAAGAGCAGATAGATCGTCAGCATGCATGGATCTACAACGCGgtgt                                                                >  2:2037389/1‑93 (MQ=255)
                                          aTTCACTGTTTGCCAGCGAAAATAATCACAACCATCAACCACAGAAAAAAAAGCAGATCGATCGCCAGCATGCCCGGAACTACAACGCGGTGTTGCAGCg                                                         >  2:666778/1‑100 (MQ=255)
                                           ttCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGAAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCgg                                                        <  2:1904913/100‑1 (MQ=255)
                                             cAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCt                                                      >  1:660613/1‑100 (MQ=255)
                                             cAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGAAAAAAAAGCAGTTCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCt                                                      <  2:2077993/100‑1 (MQ=255)
                                              aGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGTTCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTg                                                     >  1:1280805/1‑100 (MQ=255)
                                                tgtTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGcc                                                   <  1:2307238/100‑1 (MQ=255)
                                                 gtTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCg                                                  >  2:1811478/1‑100 (MQ=255)
                                                 gtTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCg                                                  <  1:320982/100‑1 (MQ=255)
                                                  tttGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGTTCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGc                                                 <  2:473289/100‑1 (MQ=255)
                                                   ttGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCa                                                <  1:319812/100‑1 (MQ=255)
                                                     gCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACg                                              <  2:1549021/100‑1 (MQ=255)
                                                     gCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACg                                              <  2:541410/100‑1 (MQ=255)
                                                       cAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGTTCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTc                                            >  1:563377/1‑100 (MQ=255)
                                                        agagTAAATAATCTCTACCATTGACCACAGCAAAAAAAGCATATCGATAGGCAGCATGCCCAGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTc                                            <  2:1339688/96‑1 (MQ=255)
                                                         gCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGc                                          <  2:1332203/100‑1 (MQ=255)
                                                           gTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGccc                                        <  1:211082/100‑1 (MQ=255)
                                                            tAAATACTCTCTACCAACGACCACAGCAAAAAAAGCAGTTCTATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGccc                                        >  2:591465/1‑99 (MQ=255)
                                                            tAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCa                                       >  2:1227002/1‑100 (MQ=255)
                                                             aaaTAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAg                                      <  2:544741/100‑1 (MQ=255)
                                                              aataatCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAgc                                     >  2:501104/1‑100 (MQ=255)
                                                               ataatCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAgcg                                    <  2:1045278/100‑1 (MQ=255)
                                                               ataatCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAgcg                                    >  1:1370220/1‑100 (MQ=255)
                                                                taatCTCTACCATCGACCACAGCAAAAAAAGCAGTTCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAgcgc                                   >  2:756165/1‑100 (MQ=255)
                                                                  atCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAgcgcgc                                 >  1:1350307/1‑100 (MQ=255)
                                                                  atCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAgcgcgc                                 >  1:659746/1‑100 (MQ=255)
                                                                    ctctACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCgcag                               >  2:1238799/1‑100 (MQ=255)
                                                                    ctctACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCgcag                               >  1:1225967/1‑100 (MQ=255)
                                                                    ctctACCATCGAACACAGCAAAAAAAGCAGATCGATCGCCAGCATACTCGGTTCTACACCGTGGGGTTGCAGCGGCTGACGCACGTCACCCAGCGCgcag                               >  2:2281666/1‑100 (MQ=255)
                                                                     tctACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCacagc                              >  1:463921/1‑100 (MQ=255)
                                                                      ctACCATCGACCACAGCAAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCgcagc                              <  1:802109/100‑1 (MQ=255)
                                                                      ctACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCgcagca                             >  1:1500074/1‑100 (MQ=255)
                                                                        aCCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCAcg                           <  1:209050/100‑1 (MQ=255)
                                                                         ccATCGACCACAGCAAAAAAAGCAGTTCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCAcgc                          <  1:1836268/100‑1 (MQ=255)
                                                                         ccATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCAcgc                          <  1:1157159/100‑1 (MQ=255)
                                                                          cATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCAcgcg                         >  1:907137/1‑100 (MQ=255)
                                                                          cATCGACCACAGCAAAAAAAGCAGATCGATAGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCAcgc                          >  2:322704/1‑99 (MQ=255)
                                                                           aTCGACCACAGCAAAAAAAGCAGTTCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCAcgcg                         <  1:85400/99‑1 (MQ=255)
                                                                           aTCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGa                        >  1:1103807/1‑100 (MQ=255)
                                                                            tCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGAt                       <  1:978899/100‑1 (MQ=255)
                                                                               aCCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCa                     <  1:888114/99‑1 (MQ=255)
                                                                               aCCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCAt                    >  1:552331/1‑100 (MQ=255)
                                                                               aCCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCAt                    >  2:338641/1‑100 (MQ=255)
                                                                                ccACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCAtg                   <  2:572915/100‑1 (MQ=255)
                                                                                  acaGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCAtgtg                 <  1:2335709/100‑1 (MQ=255)
                                                                                  acaGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCAtgtg                 <  1:1903707/100‑1 (MQ=255)
                                                                                   caGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGc                >  2:228445/1‑100 (MQ=255)
                                                                                    aGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGTGATCATGTGCg               <  2:1902971/100‑1 (MQ=255)
                                                                                     gCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCgg              <  2:333767/100‑1 (MQ=255)
                                                                                     gCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCgg              >  2:1668498/1‑100 (MQ=255)
                                                                                      cAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGAGGa             >  1:177113/1‑100 (MQ=255)
                                                                                        aaaaaaaGCAGTTCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGAc            >  1:1592195/1‑100 (MQ=255)
                                                                                        aaaaaaaGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGAc            >  2:1038594/1‑100 (MQ=255)
                                                                                          aaaaaGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCg          >  2:1121977/1‑100 (MQ=255)
                                                                                            aaaGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTg        <  1:117555/100‑1 (MQ=255)
                                                                                                cAGATCGATCGCCAGCATGCGCAGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTCCGGACCGTGaaa     >  2:750349/1‑99 (MQ=255)
                                                                                                 aGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGaaaaa   <  2:1815327/100‑1 (MQ=255)
                                                                                                  gatcgatcGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGTTCATGTGCGGACCGTGAAAAAg  <  2:514904/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
TGCTGGTGTTTACGCTGCAATCATTGACCGATCTGATGTTTAATTCAGTGTTTGCCAGCGTAAATAATCTCTACCATCGACCACAGCAAAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAG  >  NC_000913/3128765‑3128961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: