Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,150,120	T→C	16.2%	F153F (TTT→TTC)	yghA →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,150,120	0	T	C	16.2%	191.4 / 27.8	88	F153F (TTT→TTC)	yghA	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (32/41);  new base C (6/8);  total (39/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

CGAAGCGCACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCA  >  NC_000913/3150021‑3150219                                                                                                    |                                                                                                    cGAAGCGCACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGttt                                                                                                     >  2:241136/1‑100 (MQ=255)  gAAGCGCACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGttt                                                                                                     >  1:1285857/1‑99 (MQ=255)  gAAGCGCACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTc                                                                                                    <  2:2041406/100‑1 (MQ=255)   aaGCGCACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTACAGATATTAAAGACCTCACCAACAAACAGTTTCa                                                                                                   >  1:381989/1‑100 (MQ=255)      cgcACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCaaaa                                                                                                >  1:1880038/1‑100 (MQ=255)        cacaAGGCGTTAGGCGGGCTTGATATTATGGAGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGa                                                                                              <  2:1419224/100‑1 (MQ=255)          caAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGAcc                                                                                            <  2:1288026/100‑1 (MQ=255)           aaGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCt                                                                                           <  2:2014988/100‑1 (MQ=255)                gTTAGGCGGGCTGGATATTATGTCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTGGcc                                                                                      <  2:166131/100‑1 (MQ=255)                gTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGcc                                                                                      <  1:890087/100‑1 (MQ=255)                    ggCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTa                                                                                  >  1:185189/1‑100 (MQ=255)                    ggCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTa                                                                                  <  2:672980/100‑1 (MQ=255)                       gggCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAAc                                                                                <  1:902253/99‑1 (MQ=255)                       gggCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACg                                                                               <  1:772770/100‑1 (MQ=255)                       gggCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACg                                                                               <  2:2124679/100‑1 (MQ=255)                          cTGGATATTATGGCCCTGGTTGCCCTGCAACAGGTTTCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACg                                                                               <  2:858834/97‑1 (MQ=255)                             gATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTcg                                                                         <  2:1254857/100‑1 (MQ=255)                               tattatGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTcg                                                                         <  1:633834/98‑1 (MQ=255)                                  tatGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTg                                                                    <  1:1880456/100‑1 (MQ=255)                                  tatGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTACAAAAGCCCTTTGCCATTAAAGTTTTCGCGCt                                                                     >  2:1108202/1‑99 (MQ=255)                                   atGGCGCTGGTCGCCGGAAAACAAGATGCCATTCAGGATATTGCAAACCTGACCAGCGAACAGTTTCACAAGACCTTTGCGATTAACGTTGTCGCGCTGt                                                                   >  2:1652539/1‑100 (MQ=255)                                     ggCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTc                                                                 >  2:2011337/1‑100 (MQ=255)                                     ggCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTc                                                                 <  1:365578/100‑1 (MQ=255)                                        gcTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTgg                                                              <  2:306140/100‑1 (MQ=255)                                         cTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTgcc                                                             <  1:1627280/100‑3 (MQ=255)                                         cTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGc                                                             <  1:1798860/100‑1 (MQ=255)                                         cTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGc                                                             <  2:1076163/100‑1 (MQ=255)                                              cGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAAcc                                                        >  1:1913160/1‑100 (MQ=255)                                              cGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAAcc                                                        >  2:1848273/1‑100 (MQ=255)                                               gCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAAcc                                                        >  1:1241973/1‑99 (MQ=255)                                                ccGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCa                                                      <  1:2391086/100‑1 (MQ=255)                                                ccGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCa                                                      >  1:1467806/1‑100 (MQ=255)                                                   ggAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGa                                                   >  1:1536122/1‑100 (MQ=255)                                                   ggAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAAGa                                                   <  2:1101892/100‑1 (MQ=255)                                                     aaaCAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAg                                                 >  1:1713332/1‑100 (MQ=255)                                                      aaCAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGc                                                <  1:1266314/100‑1 (MQ=255)                                                       aCAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg                                               <  1:252603/100‑1 (MQ=255)                                                        cAGGTTGCCATTCCGGATATTGCAGACATCACCAGCGAAAAGTTTCAAAAGACATTTGCCATTAAAGTTTTAGCGATGTTCTGGCTAACCTAGGAAGCGa                                              >  1:554247/1‑100 (MQ=255)                                                         aGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGAt                                             <  1:2248739/100‑1 (MQ=255)                                                          ggTTGCCATTCCAGATATTACAGACGTAACCAGCGAACAGTGTCAAAAGACCTTTGCCTTAAACGTTTACGCGCTGTTCTGACTAACCCAGGAACCg                                               >  2:1744475/1‑97 (MQ=255)                                                           gTTGCCATTCCGTATATTGTAGATCTTATCAGCGAACAGTTTCAAAAGACCTTTGTCATTAACGTTTTCGTGCTGTTCTTGCTAACCCAGGAAGCGATcc                                           <  2:866164/100‑1 (MQ=255)                                                           gTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATcc                                           <  1:554909/100‑1 (MQ=255)                                                             tGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATccc                                          >  1:2003619/1‑99 (MQ=255)                                                              gCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATccccc                                        <  2:2195974/100‑1 (MQ=255)                                                              gCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATccccc                                        <  2:473438/100‑1 (MQ=255)                                                              gCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATccccc                                        <  1:2011359/100‑1 (MQ=255)                                                              gCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATacccc                                        >  1:135510/1‑100 (MQ=255)                                                               ccATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCt                                       >  1:651130/1‑100 (MQ=255)                                                               ccATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCt                                       >  1:2363752/1‑100 (MQ=255)                                                               ccATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTCTTCGCGCTGTTCTGGCTAACCCAGCAAGCGATCCCCCt                                       <  2:1474736/100‑1 (MQ=255)                                                                cATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTg                                      <  2:1011717/100‑1 (MQ=255)                                                                 aTTCCGGTTATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTgn                                     <  1:1057109/100‑2 (MQ=255)                                                                 aTTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGc                                     >  2:1775615/1‑100 (MQ=255)                                                                  ttCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCAATCCCCCTACt                                    >  1:599627/1‑100 (MQ=255)                                                                   tCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTa                                   <  1:2173440/100‑1 (MQ=255)                                                                   tCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTAGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTa                                   >  2:392479/1‑100 (MQ=255)                                                                     cGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTAcc                                 >  1:2211419/1‑100 (MQ=255)                                                                     cGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTAcc                                 <  1:138817/100‑1 (MQ=255)                                                                     cGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTAcc                                 <  1:2275822/100‑1 (MQ=255)                                                                      ggATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCGCCTGCTACCg                                >  2:703385/1‑100 (MQ=255)                                                                         tatTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGaaa                             <  2:1985432/100‑1 (MQ=255)                                                                         tatTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGaaa                             >  1:1323476/1‑100 (MQ=255)                                                                         tatTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGaaa                             >  1:2309834/1‑100 (MQ=255)                                                                          atTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAg                            >  1:2059273/1‑100 (MQ=255)                                                                           ttGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAgg                           <  1:700603/100‑1 (MQ=255)                                                                            tGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGt                          >  1:2212025/1‑100 (MQ=255)                                                                             gCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAgg                           >  2:1451851/1‑98 (MQ=255)                                                                             gCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTg                         >  1:561580/1‑100 (MQ=255)                                                                             gCAGACCTCACCAGCGAACAGTTTAAAAAGACCTTTGCCATCAACGTTTTCGCGCTGTTCTGGCTAACCCAGGACACGATCCCCCTGCTACCGAAAg                            >  2:1078892/1‑97 (MQ=255)                                                                             gCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGCTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAgg                           >  2:602362/1‑98 (MQ=255)                                                                               aGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCa                       <  2:2091871/100‑1 (MQ=255)                                                                                gACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCaa                      >  2:1715482/1‑100 (MQ=255)                                                                                 aCCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAg                     <  2:2138631/100‑1 (MQ=255)                                                                                 aCCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAg                     <  2:1783711/100‑1 (MQ=255)                                                                                   cTCACCCGCGAACAGTTTCAAAAGCCCTTTGCCGCTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGt                    <  2:2019996/99‑1 (MQ=255)                                                                                   cTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTa                   <  1:682523/100‑1 (MQ=255)                                                                                   cTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTa                   >  2:1837504/1‑100 (MQ=255)                                                                                    tCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTa                   <  1:926829/99‑1 (MQ=255)                                                                                       ccAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCACCCTGCTACCGAAAGGTGCAAGTatcat               >  2:80543/1‑100 (MQ=255)                                                                                       ccAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTatcat               >  2:395279/1‑100 (MQ=255)                                                                                         agcgctcAGTTTCAAAAGAGTTGTGCCATTAACGTTTTCGCGCTTTTCTGGCTAACCCAGGAAGTGATCCCTCTGCTACCGAAAGGt                          <  2:2029802/81‑1 (MQ=255)                                                                                         aGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTatcatc              <  1:1401775/99‑1 (MQ=255)                                                                                          ggcaaCAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTTTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTatc                 <  1:1605091/92‑1 (MQ=255)                                                                                          gCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACAGAAAGGTGCAAGGatcatc              <  2:1285844/98‑1 (MQ=255)                                                                                           cGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcacc           <  1:1702911/100‑1 (MQ=255)                                                                                           cGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcacc           >  2:2207794/1‑100 (MQ=255)                                                                                           cGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcacc           >  2:1000137/1‑100 (MQ=255)                                                                                            gAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcacca          >  2:573645/1‑100 (MQ=255)                                                                                            gAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcacca          >  1:1920959/1‑100 (MQ=255)                                                                                                aGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTc      <  1:399811/100‑1 (MQ=255)                                                                                                  tttCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCg     >  1:211570/1‑99 (MQ=255)                                                                                                   ttCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTc   <  1:613664/100‑1 (MQ=255)                                                                                                   ttCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTc   <  2:561577/100‑1 (MQ=255)                                                                                                   ttCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTc   <  1:457404/100‑1 (MQ=255)                                                                                                   ttCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTc   <  1:1836985/100‑1 (MQ=255)                                                                                                   ttCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTc   <  2:1301607/100‑1 (MQ=255)                                                                                                   ttCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTc   <  1:2089525/100‑1 (MQ=255)                                                                                                    tCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTc   <  2:662351/99‑1 (MQ=255)                                                                                                    tCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCa  <  1:708469/100‑1 (MQ=255)                                                                                                    tCAAAAGACCTTTGCCAGTAACGTTTGCGCGCTGTTCGGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCa  <  2:851244/100‑1 (MQ=255)                                                                                                    |                                                                                                    CGAAGCGCACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTCA  >  NC_000913/3150021‑3150219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑