Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,218,198 C→T 50.6% Q293Q (CAG→CAA aer ← fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,218,1980CT50.6% ‑0.8 / 112.3 77Q293Q (CAG→CAAaerfused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component
Reads supporting (aligned to +/- strand):  ref base C (19/19);  new base T (16/23);  total (35/42)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

CGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAG  >  NC_000913/3218099‑3218297
                                                                                                   |                                                                                                   
cGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGc                                                                                                     >  1:2185048/1‑100 (MQ=255)
 gCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCt                                                                                                    >  1:222029/1‑100 (MQ=255)
  cATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTg                                                                                                   <  1:717256/100‑1 (MQ=255)
    ttACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGaa                                                                                                 <  2:2155065/100‑1 (MQ=255)
     tACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAAc                                                                                                <  1:761097/100‑1 (MQ=255)
     tACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAAc                                                                                                <  1:1789219/100‑1 (MQ=255)
      aCTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACg                                                                                               <  2:708448/100‑1 (MQ=255)
         ggCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTa                                                                                            >  2:2179443/1‑100 (MQ=255)
           cAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATc                                                                                          <  1:888816/100‑1 (MQ=255)
            aGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCa                                                                                         <  2:901467/100‑1 (MQ=255)
            aGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTATCa                                                                                         <  1:563257/100‑1 (MQ=255)
             gTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCaa                                                                                        >  1:1396646/1‑100 (MQ=255)
                  tGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTc                                                                                   >  1:262311/1‑100 (MQ=255)
                     cAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTctgc                                                                                >  2:1730939/1‑100 (MQ=255)
                       gTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTctgctg                                                                              >  1:1051220/1‑100 (MQ=255)
                        tttATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTgg                                                                             <  1:843832/100‑1 (MQ=255)
                         ttATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTggg                                                                            <  2:1480492/100‑1 (MQ=255)
                         ttATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTgg                                                                             >  2:1817854/1‑99 (MQ=255)
                           aTCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTa                                                                          <  1:1174522/100‑1 (MQ=255)
                            tCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTAt                                                                         >  1:1794959/1‑100 (MQ=255)
                            tCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTAt                                                                         >  2:962539/1‑100 (MQ=255)
                            tCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTAt                                                                         >  1:1285825/1‑100 (MQ=255)
                             cGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTAt                                                                         >  1:169166/1‑99 (MQ=255)
                             cGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATg                                                                        <  2:412564/100‑1 (MQ=255)
                               gCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATgtt                                                                      <  1:909039/100‑1 (MQ=255)
                               gCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATgtt                                                                      <  2:279366/100‑1 (MQ=255)
                                cAGCCGACGCCGTGACATAGTACAGTTATACCGACGCCGCCTTTTGGTTCATTGTCCATACCGTTTGTTTAATGTTATAACGTGTCGACTGGGTATg                                                                        >  2:802313/1‑97 (MQ=255)
                                 aGCCGACGCCGTGGCAGAGTTCTGATTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACAGTCTGCTGGGTATgttc                                                                     >  2:1141628/1‑99 (MQ=255)
                                    cGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATgttcgttc                                                                 <  2:2329848/100‑1 (MQ=255)
                                    cGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATgttcgttc                                                                 <  1:1903598/100‑1 (MQ=255)
                                      aCGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATGTTCgttcag                                                               <  2:2357744/100‑1 (MQ=255)
                                       cGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATGTTCgttcagt                                                              <  1:843216/100‑1 (MQ=255)
                                        gCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCgttcagtt                                                             <  2:1500244/100‑1 (MQ=255)
                                           gTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCAt                                                          >  2:996655/1‑100 (MQ=255)
                                             ggCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATc                                                         >  1:353740/1‑99 (MQ=255)
                                              gCAGAGTTCTGTTTCACCGACGCCGTCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCgg                                                       <  2:965786/100‑1 (MQ=255)
                                              gCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCgg                                                       >  2:1181110/1‑100 (MQ=255)
                                                agagTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTg                                                     <  1:1152475/100‑1 (MQ=255)
                                                 gagTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGc                                                    <  1:13507/100‑1 (MQ=255)
                                                   gTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCt                                                  <  1:1466990/100‑1 (MQ=255)
                                                   gTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCt                                                  >  1:1980692/1‑100 (MQ=255)
                                                   gTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCCCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTccc                                                   >  2:1279453/1‑99 (MQ=255)
                                                      cTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTc                                               >  2:735219/1‑100 (MQ=255)
                                                        gTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGc                                             >  1:334495/1‑100 (MQ=255)
                                                          ttCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCa                                           >  2:2220748/1‑100 (MQ=255)
                                                           tCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAg                                          <  2:170742/100‑1 (MQ=255)
                                                             aCCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCg                                        >  2:51932/1‑100 (MQ=255)
                                                              ccGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATGTTTGTTCAGTTCATCGGTGACTTTCACCAGCGt                                       >  2:1412829/1‑100 (MQ=255)
                                                              ccGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGt                                       >  2:560536/1‑100 (MQ=255)
                                                                gACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGtct                                     <  2:1384326/100‑1 (MQ=255)
                                                                gACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGtct                                     >  1:1460502/1‑100 (MQ=255)
                                                                    ccgccATTTGGTCCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACt                                 >  2:1357706/1‑100 (MQ=255)
                                                                     cgccATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTg                                <  1:1243782/100‑1 (MQ=255)
                                                                      gccATTTGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGc                               <  1:747959/100‑1 (MQ=255)
                                                                            tGGTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTc                         <  1:1194886/100‑1 (MQ=255)
                                                                              gTTCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTg                       <  2:2207628/100‑1 (MQ=255)
                                                                              gTTCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTg                       >  2:1517845/1‑100 (MQ=255)
                                                                               ttCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGa                      <  2:353737/100‑1 (MQ=255)
                                                                               ttCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGa                      <  2:711443/100‑1 (MQ=255)
                                                                                tCATGGTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGac                     >  2:1933599/1‑100 (MQ=255)
                                                                                tCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGac                     >  1:493569/1‑100 (MQ=255)
                                                                                    ggTCGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTg                 <  1:2322852/100‑1 (MQ=255)
                                                                                    ggTCGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTg                 >  1:122265/1‑100 (MQ=255)
                                                                                    ggTCGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGATGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTg                 >  1:70518/1‑100 (MQ=255)
                                                                                       cGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGac              <  1:1254551/100‑1 (MQ=255)
                                                                                       cGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGac              >  1:2221519/1‑100 (MQ=255)
                                                                                       cGCCACCGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGac              <  2:1777983/100‑1 (MQ=255)
                                                                                       cGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGac              <  2:493061/100‑1 (MQ=255)
                                                                                       cGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGac              >  2:1886233/1‑100 (MQ=255)
                                                                                          cACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACAcc           <  2:2185022/100‑1 (MQ=255)
                                                                                            ccGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTg         <  2:1285880/100‑1 (MQ=255)
                                                                                             cGTTTGTTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTgg        <  1:2111857/100‑1 (MQ=255)
                                                                                              gTTTGCTGAACGTTATCAACTGTCTGCTGGGTGTGTTCGCTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGc       <  2:1685339/100‑1 (MQ=255)
                                                                                              gTTTGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGc       >  2:1175339/1‑100 (MQ=255)
                                                                                                ttGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCtt     <  2:161934/100‑1 (MQ=255)
                                                                                                 tGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTg    <  1:2062623/100‑1 (MQ=255)
                                                                                                 tGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTg    <  2:2027035/100‑1 (MQ=255)
                                                                                                  gCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTga   >  1:742440/1‑100 (MQ=255)
                                                                                                   cTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCCTCTCACTGCCATTTCTGACACTGGACACCTGGCTTgag  <  2:1469703/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CGCATTACTGGCAGTGATTGACAGTTTATCGGCAGCCGACGCCGTGGCAGAGTTCTGTTTCACCGACGCCGCCATTTGGTTCATGGTCGCCACCGTTTGCTGAACGTTATCAACTGTCTGCTGGGTATGTTCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAG  >  NC_000913/3218099‑3218297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: