Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,248,041 G→T 20.9% G90V (GGG→GTG)  yqjA → general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,248,0410GT20.9% 216.9 / 55.3 101G90V (GGG→GTG) yqjAgeneral envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (34/46);  new base T (10/11);  total (44/57)
Fisher's exact test for biased strand distribution p-value = 8.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.87e-01

Only 100 of 107 total aligned reads displayed.

CGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACCATCTTTTTCATAAACACGGTT  >  NC_000913/3247943‑3248139
                                                                                                  |                                                                                                  
cGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAgggg                                                                                                   <  2:2278762/100‑1 (MQ=255)
cGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAgggg                                                                                                   >  1:1088845/1‑100 (MQ=255)
cGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAgggg                                                                                                   <  1:605998/100‑1 (MQ=255)
    gtgtTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGAt                                                                                               <  1:1787118/100‑1 (MQ=255)
          aTTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTgg                                                                                         >  1:192791/1‑100 (MQ=255)
           ttGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGTGCGATGGCTggg                                                                                        <  1:720367/100‑1 (MQ=255)
           ttGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTggg                                                                                        >  1:400554/1‑100 (MQ=255)
            tGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGc                                                                                       <  1:850801/100‑1 (MQ=255)
            tGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGc                                                                                       <  1:1789018/100‑1 (MQ=255)
            tGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGc                                                                                       <  2:302201/100‑1 (MQ=255)
            tGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGc                                                                                       <  2:1486611/100‑1 (MQ=255)
            tGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGc                                                                                       <  1:1827920/100‑1 (MQ=255)
             gCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGTGCGATGGCTGGGCa                                                                                      >  1:613176/1‑100 (MQ=255)
             gCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCa                                                                                      >  1:179690/1‑100 (MQ=255)
             gCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCa                                                                                      >  1:1647069/1‑100 (MQ=255)
             gCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCa                                                                                      >  1:567758/1‑100 (MQ=255)
              cGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGTTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCaa                                                                                     >  1:1841672/1‑100 (MQ=255)
              cGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCaa                                                                                     >  2:2264313/1‑100 (MQ=255)
              cGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGCCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCa                                                                                      >  2:666103/1‑99 (MQ=255)
                 aaGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGTGCGATGGCTGGGCAATAc                                                                                  >  1:811997/1‑100 (MQ=255)
                 aaGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGTGCGATGGCTGGGCAATAc                                                                                  >  1:21052/1‑100 (MQ=255)
                     cgcgATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGTGCGATGGCTGGGCAATACCCg                                                                               <  1:818993/99‑1 (MQ=255)
                     cgcgATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGc                                                                              >  1:237262/1‑100 (MQ=255)
                      gcgATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGTGCGATGGCTGGGCAATACCCGCa                                                                             >  1:1374919/1‑100 (MQ=255)
                       cgATGGGCTATCCGCAAACGATTCTGTTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCAc                                                                            <  1:803275/100‑1 (MQ=255)
                        gATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCAcc                                                                           >  2:522984/1‑100 (MQ=255)
                          tGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGt                                                                         <  1:1474048/100‑1 (MQ=255)
                           gggCTATCCGCAAACGATTCTGCTGCTGCCCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGGTGGCTGGGCAATACCCGCACCTTa                                                                        >  1:1945843/1‑100 (MQ=255)
                           gggCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTa                                                                        <  2:1752297/100‑1 (MQ=255)
                             gCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACa                                                                      >  1:2188402/1‑100 (MQ=255)
                                aTCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACaaaa                                                                   >  2:706692/1‑100 (MQ=255)
                                  ccGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACt                                                                 >  2:1607009/1‑100 (MQ=255)
                                  ccGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACt                                                                 >  2:1946929/1‑100 (MQ=255)
                                  ccGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACt                                                                 <  1:2183587/100‑1 (MQ=255)
                                   cGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTg                                                                >  1:786273/1‑100 (MQ=255)
                                    gcgaaCGATTCTGCGGGTGAGCGTTGCCGCCAGCCTCGGCTGCTGGGGCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACt                                                                 <  2:1886880/95‑1 (MQ=255)
                                    gCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTg                                                                <  1:123676/99‑1 (MQ=255)
                                     cAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGc                                                              <  1:1420339/100‑1 (MQ=255)
                                      aaaCGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGTGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCt                                                             <  2:1770130/100‑1 (MQ=255)
                                      aaaCGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCt                                                             <  2:337568/100‑1 (MQ=255)
                                       aaCGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGTGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTa                                                            <  2:765294/100‑1 (MQ=255)
                                       aaCGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTa                                                            >  2:1176947/1‑100 (MQ=255)
                                        aCGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGTGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTAt                                                           <  1:1441936/100‑1 (MQ=255)
                                         cGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAGAACTGGCTAtc                                                          <  2:1257947/100‑1 (MQ=255)
                                         cGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTAtc                                                          >  1:1339008/1‑100 (MQ=255)
                                           aTTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGTGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTAtctc                                                        <  1:1605491/100‑1 (MQ=255)
                                           aTTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTAtctc                                                        <  2:443189/100‑1 (MQ=255)
                                            ttCTGCTGCTTACCGTTGCCGCCAGCTTCTGCTGCTGGGTCAGCTATATCCAGGTGCGATGGCTGGTCAATACCCGCACCGTACAAAACTGGCTATCTCa                                                       <  2:1574351/100‑1 (MQ=255)
                                            ttCTGCTGCTGACCGTTGCCGCTAGTTTCGGTTGCTGGGTTAGCTATATTCAGGGGCGATGGCTGGGGAATAGGCGCACCGTACAAAACTGGGTAtttta                                                       >  2:1997893/1‑96 (MQ=255)
                                            ttCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCa                                                       >  2:1919755/1‑100 (MQ=255)
                                            ttCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCa                                                       <  2:1024186/100‑1 (MQ=255)
                                             tCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGTGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCAt                                                      <  2:1012471/100‑1 (MQ=255)
                                             tCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCAt                                                      <  1:273888/100‑1 (MQ=255)
                                              ccgatcttgACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACAAAAAAAAAAAAAAACTGGCTATCTCAtt                                                     <  2:1858507/93‑1 (MQ=255)
                                                 ctgctgACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGAAGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTAc                                                  >  2:2105433/1‑100 (MQ=255)
                                                   gctgACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATATAATTTAccc                                                >  1:1393767/1‑100 (MQ=255)
                                                    ctgACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGTGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCcg                                               >  2:1108757/1‑100 (MQ=255)
                                                     tgACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCAttagatcgg                                              >  1:1858528/1‑93 (MQ=255)
                                                      gACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGTGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCcgcg                                             >  1:1018771/1‑100 (MQ=255)
                                                        ccGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTAGCTCATTTACCCGCGCa                                           >  1:2286357/1‑100 (MQ=255)
                                                         cGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGTGCGATGGCTGGGCAATACCCGCACCGTACACAACTGGCTATCTCATTTACCCACGCAt                                          >  1:35474/1‑100 (MQ=255)
                                                          gTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCAtt                                         <  1:1791734/100‑1 (MQ=255)
                                                            tGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTat                                       <  2:1088835/100‑1 (MQ=255)
                                                             gccgccAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatc                                      <  1:2392005/100‑1 (MQ=255)
                                                              ccgccAGCCTCGGCTGCTGGGTCAGCTATATTCAGGTGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatca                                     >  1:1775302/1‑100 (MQ=255)
                                                              ccgccAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatca                                     <  1:1245548/100‑1 (MQ=255)
                                                              ccgccAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatca                                     >  2:27634/1‑100 (MQ=255)
                                                              ccgccAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatca                                     <  2:1866639/100‑1 (MQ=255)
                                                              ccgccAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatca                                     <  2:1446081/100‑1 (MQ=255)
                                                              ccgccAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAAGACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatca                                     <  1:2303094/100‑1 (MQ=255)
                                                               cgccAGCCTCGGCTGCTGGGTCAGCTATATTCAGGTGCGATGGCTGGGCAATAACCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatcat                                    >  1:2399087/1‑100 (MQ=255)
                                                                gccAGCCTCGGCTGCTGGGTCATCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatcatn                                   <  1:2003121/100‑2 (MQ=255)
                                                                gccAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatcatc                                   <  1:1946951/100‑1 (MQ=255)
                                                                gccAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatcatc                                   >  2:2096422/1‑100 (MQ=255)
                                                                 ccAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCATACAAAACTGGCTATCTCCTTTACCCGCGCATTAtcatca                                  >  2:1366308/1‑100 (MQ=255)
                                                                  cAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCaa                                 <  1:522987/100‑1 (MQ=255)
                                                                   agtttCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCATCGTACAAAACTGGCTATCTCATTTACCCGCGCATTatca                                     <  1:167765/91‑1 (MQ=255)
                                                                            tgctgGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACAcc                       <  1:1016574/100‑1 (MQ=255)
                                                                            tgctgGGTCAGCTATATTCAGGGGCGAAGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACAcc                       <  1:2105456/100‑1 (MQ=255)
                                                                             gctgGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACCa                      >  2:50616/1‑100 (MQ=255)
                                                                              ctgGGTCAGCTATATTCAGGTGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACCAt                     >  2:31903/1‑100 (MQ=255)
                                                                              ctgGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACCAt                     >  1:1795915/1‑100 (MQ=255)
                                                                               tgGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACCATc                    <  1:1144605/100‑1 (MQ=255)
                                                                                gggTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCGATCTCATTTACCCGCGCAACACCATCAACGCGCACACCAt                     >  2:1500137/1‑98 (MQ=255)
                                                                                   tCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACCATCtttt                >  2:857940/1‑100 (MQ=255)
                                                                                   tCAGCTATATTAAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACCATCtttt                <  2:4490/100‑1 (MQ=255)
                                                                                    cAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACCATCttttt               <  1:1607642/100‑1 (MQ=255)
                                                                                     aGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACCATCTTTTTc              <  2:1872617/100‑1 (MQ=255)
                                                                                     aGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACCATCTTTTTc              <  1:2304296/100‑1 (MQ=255)
                                                                                      gCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACCATCTTTTTCa             <  2:1792377/100‑1 (MQ=255)
                                                                                        tataTTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACCATCTTTTTCa             <  2:1632015/98‑1 (MQ=255)
                                                                                         atatTCAGGTGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACCATCTTTTTCATaa          <  2:21051/100‑1 (MQ=255)
                                                                                         atatTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACCATCTTTTTCATaa          >  2:1164482/1‑100 (MQ=255)
                                                                                            ttgaGGGGCGATGGCTGGTCAATACCCGCACCGTACAAAACTGTCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACCATCTTTTTCATAAaca       <  2:550941/97‑1 (MQ=255)
                                                                                            ttCAGGGGCGATGGCTGGGCAATACAAGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTACCATCCACGCGCACACCATCTTTTTCATAAaca       >  2:412946/1‑100 (MQ=255)
                                                                                             tCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACCATCTTTTTCATAAacac      <  1:558649/100‑1 (MQ=255)
                                                                                               aGGGGGGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACCATCTTTTTCATAAACACg     <  2:1039426/99‑1 (MQ=255)
                                                                                               aGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACGCCATCTTTTTCATAAACACgg    <  2:1720628/100‑1 (MQ=255)
                                                                                                 gggCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACCATCTTTTTCATAAACACGGtt  <  1:63375/100‑1 (MQ=255)
                                                                                                 gggCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACCATCTTTTTCATAAACACGGtt  <  1:1024523/100‑1 (MQ=255)
                                                                                                  |                                                                                                  
CGGCGTGTTGATTGCGAAAGGCGCGATGGGCTATCCGCAAACGATTCTGCTGCTGACCGTTGCCGCCAGCCTCGGCTGCTGGGTCAGCTATATTCAGGGGCGATGGCTGGGCAATACCCGCACCGTACAAAACTGGCTATCTCATTTACCCGCGCATTATCATCAACGCGCACACCATCTTTTTCATAAACACGGTT  >  NC_000913/3247943‑3248139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: