Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,477,434 T→C 23.1% intergenic (‑15/‑206) fkpA ← / → slyX FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)/phi X174 lysis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,477,4340TC23.1% 205.7 / 63.4 118intergenic (‑15/‑206)fkpA/slyXFKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)/phi X174 lysis protein
Reads supporting (aligned to +/- strand):  ref base T (38/52);  new base C (13/14);  total (52/66)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 120 total aligned reads displayed.

CAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTATTTTC  >  NC_000913/3477335‑3477533
                                                                                                   |                                                                                                   
 aGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATc                                                                                                    <  1:685636/100‑1 (MQ=255)
   cTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCgg                                                                                                  <  1:2213816/100‑1 (MQ=255)
    ttCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAACCAGTGATTTCATCCATATCTCCAGGATCgtg                                                                                                 <  1:1620460/100‑3 (MQ=255)
     tCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCCTCCTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCCTATCTCCAGGACCg                                                                                                   >  2:1869418/1‑97 (MQ=255)
      cagcagCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGc                                                                                               <  2:172210/100‑1 (MQ=255)
       agcagcAGAAGAGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCa                                                                                              >  2:1256792/1‑100 (MQ=255)
       agcagcAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCa                                                                                              >  1:548866/1‑100 (MQ=255)
       agcagcAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCa                                                                                              <  1:2063307/100‑1 (MQ=255)
       agcagcAAAAGTGATTGGTGCATGCAGGGCAACAGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCa                                                                                              >  2:1438231/1‑100 (MQ=255)
          agcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCAtct                                                                                           >  1:1086443/1‑100 (MQ=255)
              aaaGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCAcc                                                                                       <  1:591698/100‑1 (MQ=255)
              aaaGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCAcc                                                                                       >  1:1747309/1‑100 (MQ=255)
              aaaGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCAcc                                                                                       >  2:719197/1‑100 (MQ=255)
               atgTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCAccc                                                                                      <  2:1455266/98‑1 (MQ=255)
               aaGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCAccc                                                                                      <  1:1883374/100‑1 (MQ=255)
               aaGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCAccc                                                                                      <  1:797576/100‑1 (MQ=255)
                aGTGATTGGTGCATGCAGGGCAACGGACATTGTGGTCGACAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCAcccc                                                                                     <  1:107161/100‑1 (MQ=255)
                  tGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAg                                                                                   <  2:1956214/100‑1 (MQ=255)
                  tGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAg                                                                                   <  1:1995326/100‑1 (MQ=255)
                    aTTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAggg                                                                                 >  1:671514/1‑100 (MQ=255)
                    aTTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAggg                                                                                 <  1:2318397/100‑1 (MQ=255)
                     ttGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAggg                                                                                 <  2:455108/99‑1 (MQ=255)
                     ttGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGt                                                                                >  2:1211315/1‑100 (MQ=255)
                       ggTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTa                                                                              >  2:1318951/1‑100 (MQ=255)
                       ggTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTa                                                                              <  1:1193181/100‑1 (MQ=255)
                       ggTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTa                                                                              >  1:1489143/1‑100 (MQ=255)
                         tgcatgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGGATCTCACCCCAGGGTTAAc                                                                            >  2:1035953/1‑100 (MQ=255)
                         tgcatgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAAc                                                                            <  1:1522966/100‑1 (MQ=255)
                             tgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACtatt                                                                        >  1:2120608/1‑100 (MQ=255)
                             tgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACtatt                                                                        >  1:1523078/1‑100 (MQ=255)
                             tgcaGGGCAACGGCCATTGTGGTCGACAGCAGAGTTAATTTAAACAGAGATTTCATACATATCTCCAGGAAAGGGGCATCTCACCACAGGGTTAACtatt                                                                        >  2:2033894/1‑100 (MQ=255)
                               caGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACtattat                                                                      <  1:2165604/100‑1 (MQ=255)
                               caGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACtattat                                                                      <  1:996757/100‑1 (MQ=255)
                                aGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATc                                                                     <  1:411118/100‑1 (MQ=255)
                                aGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATc                                                                     <  2:474723/100‑1 (MQ=255)
                                aGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATc                                                                     <  2:2064103/100‑1 (MQ=255)
                                aGGGCAACGGCCATTCTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATc                                                                     <  1:2364772/100‑1 (MQ=255)
                                   gccaCGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAGCAGTGATTTCATCCATATCTCCAGGATCGGGGCATGTCGCCCCAGTGTTAAc                                                                            <  2:1519903/87‑1 (MQ=255)
                                    cAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGaa                                                                 >  2:630779/1‑100 (MQ=255)
                                     aaCGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCTAGGATCGGGGCATCTCACCCCAGGGTTACCTATTATCAGagg                                                                <  1:1624017/100‑3 (MQ=255)
                                     aaCGGCCATTGTGGTCGCCAGCAGCGTTAATTTAAACAGTGATTTCATCCATATCTCCAGGATCGGTGCATCTCACCCCAGGGTTAACTATTATCAGAAg                                                                <  2:1436638/100‑1 (MQ=255)
                                       cGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAggg                                                              >  1:2295420/1‑100 (MQ=255)
                                        ggCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGt                                                             <  1:1989483/100‑1 (MQ=255)
                                         gCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTa                                                            >  1:2014969/1‑100 (MQ=255)
                                          ccAGTGGGGTCGCCAGCAGCGTTACTTTAAGCAGTGATTTCATCTATATCTCCAGGATCGGGGCGTCTCACCCCAGGGTTAACTATTATCAGAAGGGTAc                                                           <  2:2186918/100‑1 (MQ=255)
                                              tgtgGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACtata                                                       <  2:532670/100‑1 (MQ=255)
                                               gtgGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATaa                                                      >  2:1140861/1‑100 (MQ=255)
                                                 ggTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAg                                                    <  2:924249/100‑1 (MQ=255)
                                                   tCGCCAGCGGCGTTACTTTAAACAGTGATTTCATCCATATCTGCAGGATCGGGGCATCTCACCCCAGGGATAACTATTATCAGAAGGGTACTATAAAGCg                                                  <  1:2158549/100‑1 (MQ=255)
                                                   tCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAg                                                    <  1:112118/98‑1 (MQ=255)
                                                    cGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGt                                                 <  2:1195960/100‑1 (MQ=255)
                                                     gCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGtt                                                >  2:765572/1‑100 (MQ=255)
                                                      ccAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTg                                               >  2:1725383/1‑100 (MQ=255)
                                                      ccAGCACCGTTGCTTTAAACAGTGATATCATCCATAGCTCCAGGACCGGGGCATCTCTCACCAGTGTTAACTATTATCAGAAGGGTACTATAAAGCGTTg                                               >  2:2085223/1‑100 (MQ=255)
                                                       cagcagCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGt                                              <  2:1043164/100‑1 (MQ=255)
                                                        agcagcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTc                                             <  2:279089/100‑1 (MQ=255)
                                                        agcagcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTc                                             <  2:1267790/100‑1 (MQ=255)
                                                        agcagcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTc                                             <  1:2047425/100‑1 (MQ=255)
                                                          cagcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGa                                           <  1:1951733/100‑1 (MQ=255)
                                                          cagcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGa                                           <  1:2094337/100‑1 (MQ=255)
                                                          cagcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGa                                           <  2:1914090/100‑1 (MQ=255)
                                                            gcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAAc                                         <  1:334819/100‑1 (MQ=255)
                                                              gTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACaa                                       <  1:1370380/100‑1 (MQ=255)
                                                                tACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAAt                                     <  1:307242/100‑1 (MQ=255)
                                                                 aCTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATc                                    <  1:1477895/100‑1 (MQ=255)
                                                                 aCTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATc                                    >  2:400097/1‑100 (MQ=255)
                                                                 aCTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATc                                    <  2:1016101/100‑1 (MQ=255)
                                                                  cTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACaaa                                      >  2:365569/1‑97 (MQ=255)
                                                                  cTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCt                                   >  2:1745317/1‑100 (MQ=255)
                                                                  cTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCt                                   >  1:1593996/1‑100 (MQ=255)
                                                                  cTTTAAACAGTGATTTCATCCAGATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCt                                   >  2:1387226/1‑100 (MQ=255)
                                                                   tttAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCta                                  >  2:1420131/1‑100 (MQ=255)
                                                                    ttAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtac                                 >  1:509502/1‑100 (MQ=255)
                                                                     tAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtaca                                <  2:26185/100‑1 (MQ=255)
                                                                        aCAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacatac                             <  2:2049620/100‑1 (MQ=255)
                                                                         cAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacataca                            >  1:2018698/1‑100 (MQ=255)
                                                                          aGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAg                           <  2:519280/100‑1 (MQ=255)
                                                                          aGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAg                           <  1:719205/100‑1 (MQ=255)
                                                                          aGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAACCGTTGTCGAACAAATCtacataca                            >  1:959576/1‑99 (MQ=255)
                                                                           ctGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGa                          <  1:357490/99‑1 (MQ=255)
                                                                             gATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGaca                        >  2:79164/1‑100 (MQ=255)
                                                                             gATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGaag                        >  2:357487/1‑98 (MQ=255)
                                                                                 tCATCCATATCTCCAGGATCGGGGCATCGCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGcc                    <  1:1416841/100‑1 (MQ=255)
                                                                                  cATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGccc                   >  2:1326592/1‑100 (MQ=255)
                                                                                   aTCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGccc                   >  1:1332136/1‑99 (MQ=255)
                                                                                    tCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCta                 <  2:1523060/100‑1 (MQ=255)
                                                                                     ccATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATATACATACAGAAACGCCCtat                >  2:2376310/1‑100 (MQ=255)
                                                                                     ccATATCTCCAGGATCAAAGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTTGAACAAATCTACATACAGACACGACCt                  >  2:688444/1‑98 (MQ=255)
                                                                                      cATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCtatt               <  2:756327/100‑1 (MQ=255)
                                                                                         atCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatc            <  2:186878/100‑1 (MQ=255)
                                                                                          tctcCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatca           <  1:630782/100‑1 (MQ=255)
                                                                                           ctcCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatcat          <  2:1415793/100‑1 (MQ=255)
                                                                                            tcCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatcatc         <  2:1909528/100‑1 (MQ=255)
                                                                                            tcCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatcatc         >  2:91839/1‑100 (MQ=255)
                                                                                            tcCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatcat          >  1:1109596/1‑99 (MQ=255)
                                                                                             ccAGGTTCGGGGCATCTCACCACAGGGTTGACTATTATCAGAAGGGGACTATAAAGCGTTGTCGAACAAAACTACATACAGACACGCACTATTataat          >  2:1491282/1‑98 (MQ=255)
                                                                                              cAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTa       >  1:574952/1‑100 (MQ=255)
                                                                                              cAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGGACTATACAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTa       >  2:2172968/1‑100 (MQ=255)
                                                                                                 gATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTAttt    >  2:1416595/1‑100 (MQ=255)
                                                                                                   tCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTATTTTc  <  2:2386394/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CAGCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTATTTTC  >  NC_000913/3477335‑3477533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: