Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,881,837 G→T 26.7% T162N (ACC→AAC)  dnaN ← DNA polymerase III, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,881,8370GT26.7% 266.1 / 92.0 141T162N (ACC→AAC) dnaNDNA polymerase III, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (49/53);  new base T (22/16);  total (72/69)
Fisher's exact test for biased strand distribution p-value = 3.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

ACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCGGCAGGG  >  NC_000913/3881738‑3881936
                                                                                                   |                                                                                                   
aCGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCgg                                                                                                     <  2:41099/100‑1 (MQ=255)
aCGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCgg                                                                                                     <  2:1241990/100‑1 (MQ=255)
 cGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGt                                                                                                    >  1:2395678/1‑100 (MQ=255)
 cGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGt                                                                                                    <  2:1230066/100‑1 (MQ=255)
    tCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTc                                                                                                 <  2:2241511/100‑1 (MQ=255)
     cACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCa                                                                                                <  2:1041231/100‑1 (MQ=255)
     cACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCa                                                                                                >  1:1249053/1‑100 (MQ=255)
     cACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCa                                                                                                >  1:78796/1‑100 (MQ=255)
     cACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCa                                                                                                <  2:491632/100‑1 (MQ=255)
      aCCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCaa                                                                                               >  1:2157862/1‑100 (MQ=255)
       ccGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCaaa                                                                                              >  1:208143/1‑100 (MQ=255)
        cGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAAc                                                                                             <  1:1582126/100‑1 (MQ=255)
             ggCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCAt                                                                                        >  1:322506/1‑100 (MQ=255)
              gCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATa                                                                                       <  2:125681/100‑1 (MQ=255)
              gCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATa                                                                                       <  1:1970072/100‑1 (MQ=255)
               cTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATAc                                                                                      >  2:1150517/1‑100 (MQ=255)
                  ggCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCAt                                                                                   >  2:868030/1‑100 (MQ=255)
                  ggCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCAt                                                                                   >  1:657985/1‑100 (MQ=255)
                    ctaaGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCAttt                                                                                 <  1:1235049/98‑1 (MQ=255)
                     aaaGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTa                                                                                <  1:725927/100‑1 (MQ=255)
                     aaaGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTa                                                                                <  2:890450/100‑1 (MQ=255)
                       aGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCATCGTTTTCAAACAGCATACCATTTAAg                                                                              >  2:668720/1‑100 (MQ=255)
                        gATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGt                                                                             <  1:1373962/100‑1 (MQ=255)
                         aTTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTa                                                                            <  2:799510/100‑1 (MQ=255)
                         aTTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTa                                                                            <  1:1760433/100‑1 (MQ=255)
                            gACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATa                                                                         <  2:1363723/100‑1 (MQ=255)
                            gACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATa                                                                         >  2:420020/1‑100 (MQ=255)
                            gACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATa                                                                         >  1:1212252/1‑100 (MQ=255)
                             aCCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAg                                                                        <  1:109652/100‑1 (MQ=255)
                              ccAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGc                                                                       >  2:940985/1‑100 (MQ=255)
                                aaTTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGa                                                                     >  1:1667101/1‑100 (MQ=255)
                                   tGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACg                                                                  <  1:428351/100‑1 (MQ=255)
                                    ggCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGt                                                                 >  1:1451738/1‑100 (MQ=255)
                                    ggCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGt                                                                 >  1:484255/1‑100 (MQ=255)
                                    ggCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGt                                                                 >  1:1180883/1‑100 (MQ=255)
                                      cATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTcc                                                               >  2:1653136/1‑100 (MQ=255)
                                      cATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTcc                                                               >  1:2366774/1‑100 (MQ=255)
                                      cATTGAACAGACCGCCAGACGGTCGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTcc                                                               >  2:624035/1‑100 (MQ=255)
                                       aTTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCt                                                              <  1:1273898/100‑1 (MQ=255)
                                       aTTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCt                                                              >  2:1993063/1‑100 (MQ=255)
                                         tGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGa                                                            <  2:1445556/100‑1 (MQ=255)
                                          gAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGAt                                                           >  1:2223129/1‑100 (MQ=255)
                                           aaCAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATg                                                          <  1:933774/100‑1 (MQ=255)
                                             cAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATgcg                                                        <  2:1719477/100‑1 (MQ=255)
                                              aGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATgcgc                                                       <  2:1244170/100‑1 (MQ=255)
                                              aGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATgcgc                                                       <  2:347609/100‑1 (MQ=255)
                                               gACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGcc                                                      <  2:1445603/100‑1 (MQ=255)
                                               gACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGcc                                                      >  2:1805308/1‑100 (MQ=255)
                                               gACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGcc                                                      <  2:480624/100‑1 (MQ=255)
                                                aCCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCa                                                     <  2:2055560/100‑1 (MQ=255)
                                                 ccGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCAt                                                    <  1:162083/100‑1 (MQ=255)
                                                 ccGCCAGACGGTAGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCCTACCATTTAAGTAATAGCGAACGTCCTGATgcg                                                        >  2:1982979/1‑96 (MQ=255)
                                                  cGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGAGCAATa                                                   >  1:1862944/1‑100 (MQ=255)
                                                   gCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAg                                                  >  2:354553/1‑100 (MQ=255)
                                                      aGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGaaa                                               <  2:1264656/100‑1 (MQ=255)
                                                       gACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGaaaa                                              >  1:242890/1‑100 (MQ=255)
                                                       gACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGaaaa                                              >  1:2344351/1‑100 (MQ=255)
                                                         cGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACt                                            >  1:176376/1‑100 (MQ=255)
                                                          ggTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTg                                           <  1:1288819/100‑1 (MQ=255)
                                                             ggCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGt                                        <  1:1143604/100‑1 (MQ=255)
                                                               ccGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCg                                      >  1:602008/1‑100 (MQ=255)
                                                               ccGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCg                                      >  1:2141599/1‑100 (MQ=255)
                                                                cGTTGGTTGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTTCTGATGCGCCATAGAAAACTGGGTCg                                      <  2:1568433/99‑1 (MQ=255)
                                                                cGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGc                                     >  2:1361130/1‑100 (MQ=255)
                                                                  tCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCtt                                   <  2:2071910/100‑1 (MQ=255)
                                                                    ggTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCa                                 <  1:2007500/100‑1 (MQ=255)
                                                                      ttGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAAt                               <  1:1962234/100‑1 (MQ=255)
                                                                       tGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATc                              <  2:484527/100‑1 (MQ=255)
                                                                       tGCCACGGTGCGCAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATc                              >  2:1741037/1‑100 (MQ=255)
                                                                       tGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATc                              <  1:241524/100‑1 (MQ=255)
                                                                       tGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATc                              <  2:797755/100‑1 (MQ=255)
                                                                        gCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACGGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCa                             <  2:1322226/100‑1 (MQ=255)
                                                                         ccACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAg                            >  1:2073780/1‑100 (MQ=255)
                                                                         ccACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAg                            >  2:700951/1‑100 (MQ=255)
                                                                         ccACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAg                            >  1:2277184/1‑100 (MQ=255)
                                                                          cACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGa                           >  2:1859494/1‑100 (MQ=255)
                                                                            cGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACg                         <  1:1361144/100‑1 (MQ=255)
                                                                               tacgcAGTTCTTCACCTTCGTGTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATCCGCCATAGAAAACTGGATCGCTTCAATCAGACGCtt                      >  2:466223/3‑100 (MQ=255)
                                                                                gcgcAGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCCGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTc                     >  2:307370/1‑100 (MQ=255)
                                                                                gcgcAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTc                     >  2:848405/1‑100 (MQ=255)
                                                                                 cgcAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCa                    >  1:1034567/1‑100 (MQ=255)
                                                                                   cAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATc                  >  1:1487294/1‑100 (MQ=255)
                                                                                    aGTTCTTCACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCg                 <  1:680216/100‑1 (MQ=255)
                                                                                       tcttcACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTg              <  1:184625/100‑1 (MQ=255)
                                                                                       tcttcACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTg              <  1:611910/100‑1 (MQ=255)
                                                                                       tcttcACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTg              <  1:488022/100‑1 (MQ=255)
                                                                                        cttcACCTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCAATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGc             >  2:1054567/1‑100 (MQ=255)
                                                                                        cttcACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGc             <  1:320001/100‑1 (MQ=255)
                                                                                        cttcACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGc             >  1:1134852/1‑100 (MQ=255)
                                                                                         tttactttCGGTTTCAAACAGTATACCATTTAAGTAATAGCGGACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGc             <  1:41881/93‑1 (MQ=255)
                                                                                             ccTTCGTTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCg        >  1:2222555/1‑100 (MQ=255)
                                                                                             ccTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGccagat        >  1:1380913/1‑96 (MQ=255)
                                                                                             ccTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCg        >  1:1315667/1‑100 (MQ=255)
                                                                                              cTTCGGTTTCAAACAGCATACCAATAAAGTAATAACGAACGTACTGATGCGCAATAGAAAACTAGGTCGCTTCAAACAGACACTTCATCGtt               >  2:608950/1‑92 (MQ=255)
                                                                                               ttCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCGGc      <  1:1044753/100‑1 (MQ=255)
                                                                                                tCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCGGCa     <  2:194165/100‑1 (MQ=255)
                                                                                                 cGGTTTCACAACGCATACAATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCGGc      <  1:1553138/98‑1 (MQ=255)
                                                                                                  ggTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCGGCAgg   >  2:1297338/1‑100 (MQ=255)
                                                                                                   gTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCGGCAggg  >  1:788418/1‑100 (MQ=255)
                                                                                                   gTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCGGCAggg  <  1:998102/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
ACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCGGTTGCCACGGTGCGCAGTTCTTCACCTTCGGTTTCAAACAGCATACCATTTAAGTAATAGCGAACGTCCTGATGCGCCATAGAAAACTGGGTCGCTTCAATCAGACGCTTCATCGTTGCCTGCGGCAGGG  >  NC_000913/3881738‑3881936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: