Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,922,509 G→A 19.7% intergenic (‑69/+548) atpI ← / ← rsmG ATP synthase, membrane‑bound accessory factor/16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,922,5090GA19.7% 251.3 / 50.7 121intergenic (‑69/+548)atpI/rsmGATP synthase, membrane‑bound accessory factor/16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein
Reads supporting (aligned to +/- strand):  ref base G (55/41);  new base A (12/12);  total (67/54)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01

Only 100 of 131 total aligned reads displayed.

CAACGTTTCGACTCACGAGCGACACAGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGC  >  NC_000913/3922410‑3922608
                                                                                                   |                                                                                                   
cAACGTTTCGACTCACGAGCGACACAGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTagag                                                                                                     <  1:878532/100‑1 (MQ=255)
cAACGTTTCGACTCACGAGCGACACAGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTagag                                                                                                     <  1:2237441/100‑1 (MQ=255)
  aCGTTTCGACTCACGAGCGACACAGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTc                                                                                                   <  1:2047371/100‑1 (MQ=255)
    gTTTCGACTCACGAGCGACACAGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCaa                                                                                                 >  1:480582/1‑100 (MQ=255)
    gTTTCGACTCACGAGCGACACAGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCa                                                                                                  <  1:952913/99‑1 (MQ=255)
     tttCGACTCACGAGCGACACAGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCaa                                                                                                 >  1:109205/1‑99 (MQ=255)
      ttCGACTCAGTAGCGACACAGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGc                                                                                               <  2:1806508/100‑1 (MQ=255)
        cGACTCACGAGCGACACAGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCAt                                                                                             <  1:255118/100‑1 (MQ=255)
         gACTCACGAGCGACACAGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATc                                                                                            >  1:2104440/1‑100 (MQ=255)
           cTCACGAGCGACACAGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCaa                                                                                          <  1:113152/100‑1 (MQ=255)
           cTCACGAGCGACACAGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCaa                                                                                          <  1:1286418/100‑1 (MQ=255)
           cTCACGAGCGACACAGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAATCAAGCATCaa                                                                                          >  2:97/1‑100 (MQ=255)
             cACGAGCGACACAGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCACCaaaa                                                                                        >  1:1004107/1‑100 (MQ=255)
             cACGAGCGACACAGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAATCAAGCATCaaaa                                                                                        >  1:2388819/1‑100 (MQ=255)
               cGAGCGACACAGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAg                                                                                      >  1:119089/1‑100 (MQ=255)
                gAGCGACACAGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAATCAAGCATCAAAAAGc                                                                                     <  1:1706353/100‑1 (MQ=255)
                 aGCGACACAGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAATCAAGCATCTAAAAGc                                                                                     >  1:133335/1‑99 (MQ=255)
                  gCGACACAGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAATCAATCATCAAAAAGCgg                                                                                   <  1:1627358/100‑1 (MQ=255)
                    gACACAGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTc                                                                                 >  1:876708/1‑100 (MQ=255)
                    gACACAGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTc                                                                                 >  1:1716287/1‑100 (MQ=255)
                      cacaGACATCACTTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAATCAAGCATCAAAAAGCGGTCaa                                                                               <  2:1150808/100‑1 (MQ=255)
                      cacaGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCaa                                                                               >  1:2245823/1‑100 (MQ=255)
                       accgACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCTTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCaa                                                                               <  2:597553/96‑1 (MQ=255)
                        caGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAAt                                                                             >  1:1658577/1‑100 (MQ=255)
                        caGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAATCAAGCATCAAAAAGCGGTCAAAt                                                                             >  1:1492510/1‑100 (MQ=255)
                        caGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAATCAAGCATCAAAAAGCGGTCAAAt                                                                             >  1:1209572/1‑100 (MQ=255)
                          gACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATta                                                                           <  1:415329/100‑1 (MQ=255)
                            naTCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATtata                                                                         >  2:1081955/2‑100 (MQ=255)
                            cATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATtata                                                                         >  1:2201732/1‑100 (MQ=255)
                            cATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAATCAAGCATCAAAAAGCGGTCAAATtata                                                                         <  2:1720996/100‑1 (MQ=255)
                             aTCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATAc                                                                        >  1:202820/1‑100 (MQ=255)
                              tCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATAc                                                                        <  1:1277165/99‑1 (MQ=255)
                              tCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATAc                                                                        >  2:1382784/1‑99 (MQ=255)
                               cACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACgg                                                                      <  1:201476/100‑1 (MQ=255)
                               cACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAATCAAGCATCAAAAAGCGGTCAAATTATACgg                                                                      >  1:449796/1‑100 (MQ=255)
                                aCGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACgat                                                                     >  1:2069323/1‑98 (MQ=255)
                                 cGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGt                                                                     >  1:1929191/1‑99 (MQ=255)
                                 cGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCAGTCAAATTATACg                                                                       >  2:178087/1‑97 (MQ=255)
                                   tttttCACTCCTGCTCCCTTGGAGGTATGCCGCGTGTCTTATATAACTTTCTTTAAGGCTTAGAGTCAAAAATCAAAAAGCGGTCaa                                                                               <  2:1248004/87‑1 (MQ=255)
                                   tttttCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTgcg                                                                  >  1:1764800/1‑100 (MQ=255)
                                     tttCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAATCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGcc                                                                <  1:663402/100‑1 (MQ=255)
                                       tCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAGAACTTTCTTTAAGGCTTAGATTCAAGGATCAAAAAGCGGTCAAATTATACGGTGCGcccc                                                              <  2:680186/100‑1 (MQ=255)
                                       tCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGcccc                                                              >  2:2213513/1‑100 (MQ=255)
                                         aCTCCTGCTCCCTTCGAGGTATGCCGCGTGTTGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAATCTTTCAAATTATACGGTGCGCCCCCg                                                            <  2:699718/100‑1 (MQ=255)
                                         aCTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAATAAGCTGTTAAATTATACCGGGCGCCCCCg                                                            <  2:1962262/100‑1 (MQ=255)
                                           tCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCTTCAAAAAGCGGTCAAATTATACGGTacg                                                                  >  2:2051815/1‑92 (MQ=255)
                                            ccTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGa                                                         >  1:1655180/1‑100 (MQ=255)
                                                cTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTc                                                     >  1:494825/1‑100 (MQ=255)
                                                cTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTc                                                     >  2:877141/1‑100 (MQ=255)
                                                  cccTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCaa                                                   <  1:567337/100‑1 (MQ=255)
                                                    cTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCaaa                                                  >  1:118425/1‑99 (MQ=255)
                                                    cTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCaaa                                                  >  2:1954930/1‑99 (MQ=255)
                                                    cTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAAc                                                 >  1:807875/1‑100 (MQ=255)
                                                        gAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAATCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATa                                             <  2:1529866/100‑1 (MQ=255)
                                                           gTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGt                                          <  2:818124/100‑1 (MQ=255)
                                                            tATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTa                                         <  2:1806468/100‑1 (MQ=255)
                                                              tGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGc                                       >  1:518300/1‑100 (MQ=255)
                                                              tGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGc                                       <  2:1062131/100‑1 (MQ=255)
                                                              tGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAATCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGc                                       >  1:2188338/1‑100 (MQ=255)
                                                               gCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAATCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGcc                                      <  2:449793/100‑1 (MQ=255)
                                                                 cgcgTGTCGTATAAAACTTTATTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCaaa                                    >  2:80456/1‑100 (MQ=255)
                                                                    gtgtCGTATAAAACTTTCTTTAAGGCTTAGAATCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAg                                 <  2:1622784/100‑1 (MQ=255)
                                                                      gtCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGt                               <  2:699266/100‑1 (MQ=255)
                                                                       tCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTg                              <  2:1565696/100‑1 (MQ=255)
                                                                       tCGTATAAAAATTTCTTTAAGGCTTAGAGTCAAGAATTATAAAGCGGTCAAATTATATGGTGCGCCCCCGTGCTTTCAAACAATAAGTAGcaaga                                   >  2:959936/1‑91 (MQ=255)
                                                                         gTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTaaa                            >  1:1878197/1‑100 (MQ=255)
                                                                         gTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGaa                            >  1:146573/1‑100 (MQ=255)
                                                                         gTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGaa                            >  1:1878170/1‑100 (MQ=255)
                                                                          tataAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAAt                           >  2:40591/1‑100 (MQ=255)
                                                                          tataAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAAt                           <  1:101968/100‑1 (MQ=255)
                                                                           ataAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATa                          <  2:466230/100‑1 (MQ=255)
                                                                            taAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGTGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATaa                         >  2:2250129/1‑100 (MQ=255)
                                                                             aaaaCTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATaaa                        >  1:797865/1‑100 (MQ=255)
                                                                               aaCTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATg                      <  2:1921639/100‑1 (MQ=255)
                                                                                aCTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGt                     <  2:1693113/100‑1 (MQ=255)
                                                                                 ctttctttAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGtt                    >  1:325375/1‑100 (MQ=255)
                                                                                 ctttctttAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGtt                    >  1:1097597/1‑100 (MQ=255)
                                                                                 ctttctttAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGtt                    <  2:2116376/100‑1 (MQ=255)
                                                                                  tttctttAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGttt                   <  2:404874/100‑1 (MQ=255)
                                                                                   ttctttAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTa                  <  2:1409031/100‑1 (MQ=255)
                                                                                   ttctttAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTa                  <  2:1163967/100‑1 (MQ=255)
                                                                                    tctttAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTaa                 <  2:920312/100‑1 (MQ=255)
                                                                                    tctttAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTaa                 <  1:259922/100‑1 (MQ=255)
                                                                                     ctttAAGGCTTAGAATCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTaaa                <  1:1159140/100‑1 (MQ=255)
                                                                                      tttAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTaaa                >  1:347562/1‑99 (MQ=255)
                                                                                       ttAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAAATATCCGCTGAACCACCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTaaa                <  2:1862707/98‑1 (MQ=255)
                                                                                         aaGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAatat             <  1:558053/99‑1 (MQ=255)
                                                                                          aGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATAttt           <  1:2332567/100‑1 (MQ=255)
                                                                                           ggCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATAtttt          <  2:956363/100‑1 (MQ=255)
                                                                                           ggCTTAGAATCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATAtttt          >  2:386352/1‑100 (MQ=255)
                                                                                             cTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATAtttt          <  2:216187/98‑1 (MQ=255)
                                                                                              ttAGAGTCAAGCATCAAAAAGCGGTCAAATTATATGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTc        <  2:2195443/99‑1 (MQ=255)
                                                                                              ttAGAGTCAAGCATCAAAAAGCGGTCAAATTAGACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTcc       <  2:514840/100‑1 (MQ=255)
                                                                                               tAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCa      >  1:268346/1‑100 (MQ=255)
                                                                                                 gagTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAg     >  1:1101040/1‑99 (MQ=255)
                                                                                                 gagTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGt    >  2:734627/1‑100 (MQ=255)
                                                                                                  agTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTg   <  2:2046133/100‑1 (MQ=255)
                                                                                                  agTCAAGCATAAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTg   <  2:915087/100‑1 (MQ=255)
                                                                                                   gTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGc  >  1:1652000/1‑100 (MQ=255)
                                                                                                   gTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCCCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGc  >  1:1433981/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CAACGTTTCGACTCACGAGCGACACAGACATCACGTTTTTCACTCCTGCTCCCTTCGAGGTATGCCGCGTGTCGTATAAAACTTTCTTTAAGGCTTAGAGTCAAGCATCAAAAAGCGGTCAAATTATACGGTGCGCCCCCGTGATTTCAAACAATAAGTAGCCAAAAGGTGAATAAATGTTTAAATATTTTTCCAGTGC  >  NC_000913/3922410‑3922608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: