Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,971,690 A→C 100% Q144P (CAG→CCG)  wecC → UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,971,6900AC98.2% 364.7 / ‑3.2 110Q144P (CAG→CCG) wecCUDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (48/59);  total (49/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

Only 100 of 116 total aligned reads displayed.

GCGCGCTGGTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCAGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCG  >  NC_000913/3971594‑3971785
                                                                                                |                                                                                               
gcgcgcTGGTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCgccgca                                                                                              <  2:2201642/100‑1 (MQ=255)
 cgcgcTGGTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGccgcag                                                                                             >  2:609846/1‑100 (MQ=255)
  gcgcTGGTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAgg                                                                                            <  2:491605/100‑1 (MQ=255)
   cgcTGGTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCgccgca                                                                                              >  2:1571803/1‑97 (MQ=255)
   cgcTGGTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGt                                                                                           >  1:1605275/1‑100 (MQ=255)
     cTGGTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCCGAATGGGTGGCAGAGATGCGTGCGGATCTCACTTTCCcgccgc                                                                                               >  2:1402115/1‑94 (MQ=255)
        gTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCg                                                                                      <  1:2093135/100‑1 (MQ=255)
          gATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAg                                                                                    >  1:1779827/1‑100 (MQ=255)
           aTCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGc                                                                                   <  1:947093/100‑1 (MQ=255)
            tCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCa                                                                                  <  1:848464/100‑1 (MQ=255)
             ccTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAg                                                                                 >  1:966372/1‑100 (MQ=255)
              cTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAgg                                                                                >  1:1570817/1‑100 (MQ=255)
              cTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAg                                                                                 >  1:796081/1‑99 (MQ=255)
               ttGAATCCTCCTTGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGc                                                                               <  1:1207204/100‑1 (MQ=255)
                 gattCCACCTCGCCGGTGGGGTCAACCGAGAAGACGGCAGAATCGTGCGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGCGCAGGCgg                                                                             <  2:2288154/97‑1 (MQ=255)
                 gAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCgg                                                                             <  2:1780056/100‑1 (MQ=255)
                 gAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCACACTTTCCCGCCGCAGGTGGGCGAGCAGGCgg                                                                             >  2:82585/1‑100 (MQ=255)
                   aTCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAgatcggaag                                                                            >  2:1524130/1‑91 (MQ=255)
                     ccACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGCACGGTTAGCAGAGCTGCGTCCGGAACTCACTTTCCCGCCGCAGGCGGGAGAGCAGGCCGAc                                                                           >  2:678539/1‑98 (MQ=255)
                      cACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTc                                                                        >  1:2101086/1‑100 (MQ=255)
                       aCCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCa                                                                       >  1:954571/1‑100 (MQ=255)
                       aCCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCa                                                                       <  2:2054031/100‑1 (MQ=255)
                       aCCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCa                                                                       <  1:2025765/100‑1 (MQ=255)
                        ccTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCaa                                                                      >  1:220694/1‑100 (MQ=255)
                         cTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAAc                                                                     >  1:922898/1‑100 (MQ=255)
                         cTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAAc                                                                     >  2:1800762/1‑100 (MQ=255)
                          tCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACa                                                                    <  2:556017/100‑1 (MQ=255)
                           cGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAAc                                                                     <  1:276080/98‑1 (MQ=255)
                           cGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACAt                                                                   >  2:1637359/1‑100 (MQ=255)
                             ccGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTGATCATTg                                                                 >  2:745014/1‑100 (MQ=255)
                             ccGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTg                                                                 >  1:184370/1‑100 (MQ=255)
                                gTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCtt                                                              <  1:671288/100‑1 (MQ=255)
                                gTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCtt                                                              <  1:2105086/100‑1 (MQ=255)
                                  ggggTCAACCGAGAAAATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTAc                                                            <  2:553648/100‑1 (MQ=255)
                                   gggTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACt                                                           >  1:1234596/1‑100 (MQ=255)
                                    ggTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGAGGGCGAGCAAGCGGACGTCAACATTGCTTACTg                                                          >  2:2057755/1‑100 (MQ=255)
                                      tCAACCGAGAAGATGGCAGAATGGTTAGCAGATATCCCTCCGGATCTCACTTTCCCGCCGCAGGTGAGCGATCCGGCGGCCGTCAACATTGCTTACTg                                                          >  2:1764886/1‑98 (MQ=255)
                                       cAACCGAGATGATTGCAGAATGTTTAGCATAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGccc                                                       <  2:1521708/100‑1 (MQ=255)
                                       cAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCCTCCGGATATCACATTCCCGCCGCAGGTGTGCGAGCAGGCGGACGTCAACATTGCTTACTGccc                                                       >  2:1488981/1‑100 (MQ=255)
                                        aaCCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCg                                                      >  2:2195489/1‑100 (MQ=255)
                                         aCCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCgg                                                     >  1:1846998/1‑100 (MQ=255)
                                            gagaAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCgg                                                     <  1:1366928/97‑1 (MQ=255)
                                            gagaAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAAc                                                  >  2:1734342/1‑100 (MQ=255)
                                               aagaTGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAATATTGCTTACTGCCCGGAAcgcg                                               <  2:1391716/100‑1 (MQ=255)
                                               aagaTGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAAcgcg                                               <  1:2373917/100‑1 (MQ=255)
                                               aagaTGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAAcgcg                                               <  1:1637379/100‑1 (MQ=255)
                                                agaTGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAAcgcg                                               <  1:1896/99‑1 (MQ=255)
                                                 gaTGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCgtg                                             <  2:1265246/100‑1 (MQ=255)
                                                 gaTGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCgtg                                             <  2:2306037/100‑1 (MQ=255)
                                                 gaTGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCgt                                              >  1:1831014/1‑99 (MQ=255)
                                                   tGGCAGAATGGTTAGTAGCGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCgtg                                             <  1:1501741/98‑1 (MQ=255)
                                                   tGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGtt                                           <  2:1942156/100‑1 (MQ=255)
                                                   tGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGtt                                           <  2:1241593/100‑1 (MQ=255)
                                                   tGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGtt                                           >  1:694302/1‑100 (MQ=255)
                                                   tGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGtt                                           >  2:1088513/1‑100 (MQ=255)
                                                    ggCAGAATGGTTAGCAGAGATGCGTCCGGATCTTACTGTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTa                                          <  2:694294/100‑1 (MQ=255)
                                                    ggCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTa                                          <  2:1163640/100‑1 (MQ=255)
                                                    ggCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTa                                          >  1:1671005/1‑100 (MQ=255)
                                                     gCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAAAATTGCTTACTGCCCGGAACGCGTGTTa                                          >  2:1721192/1‑99 (MQ=255)
                                                      cAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTAcc                                        <  2:1447680/100‑1 (MQ=255)
                                                      cAGAATGGTTAGCAGAGATGCGTCAGGATCTCACTTTCCAGCAGCAGGTAGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTa                                          >  2:1295622/1‑98 (MQ=255)
                                                      cAGAATGGTTAGAAGAGATGCGTCCGGATCTGACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGGGAACATTGCTTACTGCCCGGAACGCGTGTTAcc                                        >  2:793548/1‑100 (MQ=255)
                                                        gAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAg                                      >  1:1463187/1‑100 (MQ=255)
                                                        gAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAg                                      <  2:1846977/100‑1 (MQ=255)
                                                             gTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAg                                 <  2:647416/100‑1 (MQ=255)
                                                              ttAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTTCTTACTGCCCGGAACGCGTGTTACCAGGACAgg                                >  1:306556/1‑100 (MQ=255)
                                                               tatccgcgaTGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTTCCCGGATCGCGTGTTACCAGGACAGGt                               <  2:1466223/93‑1 (MQ=255)
                                                               tAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGt                               >  1:1575137/1‑100 (MQ=255)
                                                                  cAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTGACCAGGACAGGTAAt                            <  2:384626/100‑1 (MQ=255)
                                                                   agcgaTGCGTCCGGATCTCACTGGCCCGCGGCGGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAAt                            <  2:2383060/96‑1 (MQ=255)
                                                                   agagATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATg                           >  1:1173645/1‑100 (MQ=255)
                                                                    gagaTGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATgg                          <  1:733139/100‑1 (MQ=255)
                                                                     agaTGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGATCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGt                         <  2:954717/100‑1 (MQ=255)
                                                                      gttGCGTCCGGATCTCACTTTCCCGCCGCAGTTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGt                         <  2:806298/97‑1 (MQ=255)
                                                                      gaTGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCTGAACGCGTGTTACCAGGACAGGTAATGGt                         <  2:408115/99‑1 (MQ=255)
                                                                      gaTGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTc                        >  2:1312097/1‑100 (MQ=255)
                                                                        tGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGa                      <  1:45212/100‑1 (MQ=255)
                                                                            tCCGGATCTCACTTTCCCGCAGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTCACCAGGACAGGTAATGGTCGAGCTg                  <  1:50186/100‑1 (MQ=255)
                                                                              cGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGAt                >  2:1526564/1‑100 (MQ=255)
                                                                               ggATCTCACTTTCCCGCCGCAGGTGGGGGAGCAGGGGGACGTCAACATTGGTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGAt                <  2:2272033/99‑1 (MQ=255)
                                                                               ggATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGAtt               <  2:410407/100‑1 (MQ=255)
                                                                                gATCTCCCTTTCCCTCCGCAGTTTGGCGAGCAGGCGGATGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCt                   <  2:1894787/95‑1 (MQ=255)
                                                                                gATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTa              <  2:2120794/100‑1 (MQ=255)
                                                                                 aTCTCACTTTTCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATTGTCGAGCTGATTa              >  2:164249/1‑99 (MQ=255)
                                                                                 aTCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTaa             <  2:1255225/100‑1 (MQ=255)
                                                                                 aTCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTaa             >  1:1801513/1‑100 (MQ=255)
                                                                                  tctcACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGAtt               <  2:1499168/97‑1 (MQ=255)
                                                                                  tctcACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTaaa            >  1:2264863/1‑100 (MQ=255)
                                                                                   ctcACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTaaaa           >  1:1552043/1‑100 (MQ=255)
                                                                                     cACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAAc         <  2:552178/100‑1 (MQ=255)
                                                                                     cACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAAc         <  2:1732920/100‑1 (MQ=255)
                                                                                      aCTTTCCCGCCGCAGGTGGGCGAGCAGGCGGTCGTCAACATTGGTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAAc         <  2:2177305/99‑1 (MQ=255)
                                                                                      aCTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACg        >  2:285138/1‑100 (MQ=255)
                                                                                       cTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACCTTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGa       >  1:1193655/1‑100 (MQ=255)
                                                                                       cTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGa       >  1:1737488/1‑100 (MQ=255)
                                                                                        tttCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCGGGACAGGTAATGGTCGAGCTGATTAAAAACGAt      <  1:1887845/100‑1 (MQ=255)
                                                                                        tttCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGAt      >  2:1445290/1‑100 (MQ=255)
                                                                                          tCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATcg    <  1:86187/100‑1 (MQ=255)
                                                                                            ncgccgcAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATcgcg  >  2:1283499/2‑100 (MQ=255)
                                                                                            ccgccgCAGGTGGGCGAGCAGGCGGACGTCACCATTGCTTACTGCCCGGACCGCGTGATACCAGGACAGGCAATCGTCGAGCTGATTAAACCCGATcgcg  >  2:2335424/1‑100 (MQ=255)
                                                                                                |                                                                                               
GCGCGCTGGTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCAGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCG  >  NC_000913/3971594‑3971785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: