Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,098,512 C→A 18.2% R259R (CGC→CGA rhaS → transcriptional activator of rhaBAD and rhaT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,098,5120CA18.2% 317.7 / 48.6 126R259R (CGC→CGArhaStranscriptional activator of rhaBAD and rhaT
Reads supporting (aligned to +/- strand):  ref base C (57/44);  new base A (11/12);  total (69/57)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 131 total aligned reads displayed.

GAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAA  >  NC_000913/4098413‑4098611
                                                                                                   |                                                                                                   
gaAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGc                                                                                                     >  2:1738519/1‑100 (MQ=255)
  aaGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGATACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCg                                                                                                   <  1:2288305/100‑1 (MQ=255)
    gCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCgag                                                                                                 >  1:2191121/1‑100 (MQ=255)
    gCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCgag                                                                                                 <  1:1788778/100‑1 (MQ=255)
    gCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCgag                                                                                                 >  1:382497/1‑100 (MQ=255)
    gCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGCGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCgag                                                                                                 >  2:1832096/1‑100 (MQ=255)
     cccGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACgaga                                                                                                >  1:1144226/1‑100 (MQ=255)
      ccGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGagag                                                                                               <  1:1738540/100‑1 (MQ=255)
        gACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGtt                                                                                             <  2:1887422/100‑1 (MQ=255)
            tCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAAc                                                                                         >  1:1502742/1‑100 (MQ=255)
            tCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAAc                                                                                         <  1:1665844/100‑1 (MQ=255)
              tGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTg                                                                                       <  1:1300193/100‑1 (MQ=255)
              gctgaatccacagcgataggcgatgtcagtaacgctggcctcgctgtggATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAAATg                                                                                       >  1:118582‑M2/50‑100 (MQ=255)
               gCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTgg                                                                                      <  2:724367/100‑1 (MQ=255)
               gCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTgg                                                                                      <  2:1056376/100‑1 (MQ=255)
               gCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTgg                                                                                      >  1:2267562/1‑100 (MQ=255)
               gCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTgg                                                                                      >  2:61994/1‑100 (MQ=255)
               gCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTgg                                                                                      <  1:2170641/100‑1 (MQ=255)
                 tACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTc                                                                                    >  1:1914166/1‑100 (MQ=255)
                 tACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGCTTAACTGGTc                                                                                    >  2:2220572/1‑100 (MQ=255)
                 tACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTc                                                                                    >  1:1677684/1‑100 (MQ=255)
                  aCGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCa                                                                                   >  2:1722933/1‑100 (MQ=255)
                   cGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCAc                                                                                  >  1:2223595/1‑100 (MQ=255)
                   cGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCAc                                                                                  <  2:2290242/100‑1 (MQ=255)
                   cGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCAc                                                                                  <  2:1144215/100‑1 (MQ=255)
                   atccacagcgataggcgatgtcagtaacgctggcctcgctgtggATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCAc                                                                                  >  1:417366‑M2/45‑100 (MQ=255)
                    gCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCAcc                                                                                 >  2:1004339/1‑100 (MQ=255)
                     ccacagcgataggcgatgtcagtaacgctggcctcgctgtggATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGACAGCTTAACTGGTCAcc                                                                                 >  2:216799‑M2/43‑99 (MQ=255)
                     CCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACcg                                                                                <  2:387636‑M1/100‑1 (MQ=255)
                      CACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGAAAGTAACCACTTTTCTACGCTTTTGCGCCGAGAGTGTAACTGGTCACcg                                                                                <  2:1327255‑M1/99‑1 (MQ=255)
                       acagcgataggcgatgtcagtaacgctggcctcgctgtggATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACcgcg                                                                              >  1:148601‑M2/41‑100 (MQ=255)
                       ACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACcgcg                                                                              >  1:1132374‑M1/1‑100 (MQ=255)
                        caGCGAGGCCAGCGTTACTTACATCGCCTATCGCTGTGTATGCAGCGACATTAACCACTTTTCGACGCTTTTTCGACTATAGTTTAACTGGTCACCGCGt                                                                             <  2:225569/100‑1 (MQ=255)
                         AGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTg                                                                            >  2:898744‑M1/1‑100 (MQ=255)
                          GCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGa                                                                           >  2:1761145‑M1/1‑100 (MQ=255)
                          GCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGa                                                                           <  1:1662203‑M1/100‑1 (MQ=255)
                          GCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGa                                                                           >  1:498529‑M1/1‑100 (MQ=255)
                           CGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGat                                                                          >  1:2026869‑M1/1‑100 (MQ=255)
                            GAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGata                                                                         <  1:666584‑M1/100‑1 (MQ=255)
                            GAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGata                                                                         <  2:1903763‑M1/100‑1 (MQ=255)
                              ggCCAGCGTTACTGACATCGCCTATCGCTGTTGATTCTGCGACAGTAACCACTTTTCTACGCTTTTTCGGCGAGAGTTTAACTGGGCACCGCGTg                                                                            <  2:958276/95‑1 (MQ=255)
                                     gTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAgg                                                                <  1:2042388/100‑1 (MQ=255)
                                     gTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAg                                                                 <  2:2102985/99‑1 (MQ=255)
                                     gTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAAACCTTTTTCACGCTTTTTCGGAGAGAGGTTAAATTGTCAACGCCTGATAtttcccaagg                                                               >  2:1305222/1‑92 (MQ=255)
                                       tACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGa                                                              <  2:143130/100‑1 (MQ=255)
                                          tGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACgg                                                           <  1:950914/100‑1 (MQ=255)
                                           gACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTAGTCACCGAGAGATATTCACCAGGGAAggg                                                          >  1:5839/1‑100 (MQ=255)
                                           gACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACggg                                                          >  1:1751653/1‑100 (MQ=255)
                                             cATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGAt                                                        >  2:1444559/1‑100 (MQ=255)
                                             cATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGAt                                                        >  2:1620398/1‑100 (MQ=255)
                                              aTCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGAt                                                        >  2:662080/1‑99 (MQ=255)
                                                    tATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGGTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTc                                                 >  1:1924559/1‑100 (MQ=255)
                                                     aTCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGGGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCt                                                >  1:2404777/1‑100 (MQ=255)
                                                      tcgctgtggATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTg                                               <  2:77312‑M2/91‑1 (MQ=255)
                                                      tcgctgtggATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTg                                               <  2:862213‑M2/91‑1 (MQ=255)
                                                        gctgtggATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCa                                             <  2:599042‑M2/93‑1 (MQ=255)
                                                        gctgtggATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCCCGTGATATTCGCCACGGACGGGATGGCATTCTGCa                                             >  1:474273‑M2/8‑100 (MQ=255)
                                                         ctgtggATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCaa                                            >  1:1900946‑M2/7‑100 (MQ=255)
                                                           gtggATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTGTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATa                                          >  1:2036679‑M2/5‑100 (MQ=255)
                                                             ggATTCAGCGACAGTAACCACTTTTCGACGCTTTGTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCTAGTTACGGGATGGCTTTCGGCAATAAc                                        <  2:971873‑M2/98‑1 (MQ=255)
                                                              gATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACg                                       <  1:278743‑M2/99‑1 (MQ=255)
                                                               aTTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAAcgc                                      >  1:638565/1‑100 (MQ=255)
                                                                ttCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAAcgcg                                     <  2:2226178/100‑1 (MQ=255)
                                                                  cAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGaa                                   >  2:1525184/1‑100 (MQ=255)
                                                                  cAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGaa                                   >  2:1784827/1‑100 (MQ=255)
                                                                   aGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAAt                                  >  1:2220873/1‑100 (MQ=255)
                                                                   aGCGACAGTAACCACTTTTCGAAACTTATTCGCCGAGAGTTTAACTGGTCACCGAGTGATATTCGCCAGGGACGGGATGGCTATCTGTAATAAAGCGa                                    >  2:370776/1‑98 (MQ=255)
                                                                    gCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAAtc                                 <  2:1829180/100‑1 (MQ=255)
                                                                     cGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAAtct                                >  2:647427/1‑100 (MQ=255)
                                                                     cGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAAtct                                >  2:2337406/1‑100 (MQ=255)
                                                                       aCAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAAtcttc                              <  1:494683/100‑1 (MQ=255)
                                                                       aCAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAAtcttc                              <  2:1858344/100‑1 (MQ=255)
                                                                        cAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAAtattct                             >  1:2162430/1‑100 (MQ=255)
                                                                         aGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTtctc                            <  2:726783/100‑1 (MQ=255)
                                                                         aGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTtctc                            <  2:968398/100‑1 (MQ=255)
                                                                          gTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCa                           >  1:113182/1‑100 (MQ=255)
                                                                          gTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCa                           >  2:1744792/1‑100 (MQ=255)
                                                                          gTAACCACTTTTAGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTTGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAAtctt                               >  2:2183060/1‑96 (MQ=255)
                                                                             aCCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACg                        <  1:358151/100‑1 (MQ=255)
                                                                              ccACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACg                        <  2:1825853/99‑1 (MQ=255)
                                                                               cccTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGACACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAAc                         >  1:1625794/3‑97 (MQ=255)
                                                                               cACTTTTCGACGCTTTTTCGCTGAGAGTGTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTa                      <  2:2276960/100‑1 (MQ=255)
                                                                                 cTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTAtt                    >  1:1285896/1‑100 (MQ=255)
                                                                                   tttCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTg                  <  1:690678/100‑1 (MQ=255)
                                                                                       gACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACg              <  2:2384261/100‑1 (MQ=255)
                                                                                       gACGCTTTTTCGCAGAGAGTTTAACTGGTCACCGCGTTATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACg              <  2:1172299/100‑1 (MQ=255)
                                                                                         cGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGcc            <  1:1722954/100‑1 (MQ=255)
                                                                                         cGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGcc            >  1:1267275/1‑100 (MQ=255)
                                                                                         cGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGcc            <  2:1667040/100‑1 (MQ=255)
                                                                                         cGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGcc            <  2:1225001/100‑1 (MQ=255)
                                                                                          gCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCa           <  2:2188359/100‑1 (MQ=255)
                                                                                           cTTTTTTGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCa           <  1:57170/99‑1 (MQ=255)
                                                                                           cTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCat          <  2:1373622/100‑1 (MQ=255)
                                                                                             ttttCGCCGAGAGTTTAACTCATCACCACGTTCTATTCGCCAGGAACAGGATGGCATTCTACAATAACGCGAATCTTCTCAAAGTATTTGTACGCCatat        >  2:1756874/1‑100 (MQ=255)
                                                                                                 cGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCg    <  2:1260676/100‑1 (MQ=255)
                                                                                                  gCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGa   >  1:75918/1‑100 (MQ=255)
                                                                                                  gCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGa   >  2:1219872/1‑100 (MQ=255)
                                                                                                  gCCGAGAGTTTAACTGGTCACCACGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGa   >  2:1389168/1‑100 (MQ=255)
                                                                                                   ccGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATTCCGCGAATCTTCTCAACGTATTTGTACGCCATATTGc     <  2:2389935/97‑1 (MQ=255)
                                                                                                   ccGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGCACGCCATATTGCGaa  >  1:2364968/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
GAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAA  >  NC_000913/4098413‑4098611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: