BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,168,540	T→A	100%	intergenic (+93/‑101)	gltT → / → rrlB	tRNA‑Glu/23S ribosomal RNA of rrnB operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,168,540	0	T	A	100.0%	378.3 / ‑6.7	146	intergenic (+93/‑101)	gltT/rrlB	tRNA‑Glu/23S ribosomal RNA of rrnB operon
Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (77/68); minor base C (0/1); total (77/69)

Only 100 of 146 total aligned reads displayed.

CGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGG  >  NC_000913/4168443‑4168634

cGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACa                                                                                              <  1:1607848/100‑1 (MQ=255)

  ccACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAg                                                                                            >  1:82433/1‑100 (MQ=255)

  ccACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAg                                                                                            >  2:1830460/1‑100 (MQ=255)

   cACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGc                                                                                           <  1:1079797/100‑1 (MQ=255)

    aCTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCt                                                                                          >  1:694549/1‑100 (MQ=255)

    aCTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCt                                                                                          <  2:1704675/100‑1 (MQ=255)

       tGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGaa                                                                                       >  2:2227707/1‑100 (MQ=255)

       tGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGa                                                                                        >  1:803062/1‑99 (MQ=255)

         cTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGaaaa                                                                                     <  2:1141654/100‑1 (MQ=255)

          tGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAAt                                                                                    <  1:1040806/100‑1 (MQ=255)

           ggTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAAtt                                                                                   <  2:1799206/100‑1 (MQ=255)

           ggTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAAtt                                                                                   >  2:1793181/1‑100 (MQ=255)

            gTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTg                                                                                  >  1:1696357/1‑100 (MQ=255)

             tttGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGa                                                                                 <  2:1410903/100‑1 (MQ=255)

              ttGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGaa                                                                                >  2:324464/1‑100 (MQ=255)

              ttGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGaa                                                                                <  1:2069159/100‑1 (MQ=255)

              ttGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGaa                                                                                <  1:1835342/100‑1 (MQ=255)

                gtgagtgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAac                                                                              >  1:220888/1‑100 (MQ=255)

                gtgagtgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAac                                                                              >  2:230278/1‑100 (MQ=255)

                 tgagtgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAaca                                                                             <  1:1529588/100‑1 (MQ=255)

                  gagtgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAacac                                                                            <  2:1958835/100‑1 (MQ=255)

                  gagtgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAacac                                                                            >  1:1595203/1‑100 (MQ=255)

                    gtgaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGATATATTTGCTCTTTAAAAATCTGGACCAAGCTGAAAATTGAAACACTg                                                                          <  2:908339/100‑1 (MQ=255)

                      gaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGaa                                                                        >  2:1775161/1‑100 (MQ=255)

                      gaAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGa                                                                         <  1:504788/99‑1 (MQ=255)

                        aaGTCGCCGACCTTAATATCTCACAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGaaca                                                                      >  2:2154305/1‑100 (MQ=255)

                         aGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGaacaa                                                                     <  1:763792/100‑1 (MQ=255)

                          gTCGCCGTCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTTAGATATTTGATCTTTAAAAATCTGAAATAAGCTGAAAATTGAAACACTGaacaac                                                                    >  2:664341/1‑100 (MQ=11)

                          gTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGaacaac                                                                    >  2:1175179/1‑100 (MQ=255)

                            cGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACga                                                                  >  1:2103981/1‑100 (MQ=25)

                            cGCCGACCTTAATATCTCAAAACTCATCATAGGGTAATGTTTGAGATATTTGCTCTTTAAAAATCAGGAAAAAGCTGAAAATTGAAACACTGAACAACga                                                                  >  2:851213/1‑100 (MQ=11)

                             gCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACgag                                                                 <  1:879500/100‑1 (MQ=25)

                               cGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGagag                                                               >  1:107039/1‑100 (MQ=25)

                                gACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGaacaactctcgt                                                              <  2:413139/100‑7 (MQ=255)

                                 aCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAgtt                                                             <  1:114922/100‑1 (MQ=18)

                                    ttAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAgttgtt                                                          >  1:1825750/1‑100 (MQ=17)

                                      aaTATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCg                                                        >  2:345404/1‑100 (MQ=18)

                                      aaTATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCg                                                        <  2:1216655/100‑1 (MQ=18)

                                      aaTATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCg                                                        <  1:307901/100‑1 (MQ=18)

                                       atatCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCg                                                        <  1:785850/99‑1 (MQ=18)

                                        tatCTCACAACTCCTCTTCGGGTGATGTTTGAGATATTTGCTCTTTACAAATCTGGAACCAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTg                                                      >  2:842294/1‑100 (MQ=11)

                                        tatCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTg                                                      <  1:1734150/100‑1 (MQ=18)

                                         atCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGa                                                     >  1:2335142/1‑100 (MQ=18)

                                          tctcAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAg                                                    >  1:222601/1‑100 (MQ=17)

                                             cAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGATGTTCGTGAg                                                    >  1:34650/1‑97 (MQ=12)

                                              aaaaCTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGtctc                                                <  1:215407/100‑1 (MQ=18)

                                                aaCTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGtctctc                                              >  2:1088714/1‑100 (MQ=18)

                                                  cTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCaa                                            >  1:2387051/1‑100 (MQ=18)

                                                  cTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCaa                                            >  2:481326/1‑100 (MQ=18)

                                                  cTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCaa                                            <  2:609843/100‑1 (MQ=17)

                                                     aTCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAAtt                                         >  2:657957/1‑100 (MQ=18)

                                                     aTCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAAtt                                         <  2:1696337/100‑1 (MQ=18)

                                                      tCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAAttt                                        >  2:2015501/1‑100 (MQ=17)

                                                      tCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAAttt                                        >  1:2306914/1‑100 (MQ=17)

                                                      tCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAAttt                                        >  1:1466195/1‑100 (MQ=18)

                                                         tCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCg                                     <  2:322372/100‑1 (MQ=18)

                                                           gggTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCa                                   >  2:77976/1‑100 (MQ=18)

                                                            ggTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCaa                                  >  2:151011/1‑100 (MQ=18)

                                                             gTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAATCACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAac                                 <  2:2141217/100‑1 (MQ=12)

                                                             gTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAac                                 <  2:2343322/100‑1 (MQ=17)

                                                              tGATGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAaca                                <  2:815344/100‑1 (MQ=18)

                                                                ntGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACg                              >  1:901139/2‑100 (MQ=18)

                                                                 tGTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACga                             <  1:759107/100‑1 (MQ=18)

                                                                  gTTTGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACgat                            >  2:1060899/1‑100 (MQ=17)

                                                                   tttGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACgatg                           <  2:2100309/100‑1 (MQ=18)

                                                                   tttGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACgatg                           <  2:534955/100‑1 (MQ=17)

                                                                     tGAGATATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACgatgat                         <  1:454865/100‑1 (MQ=17)

                                                                      gagaTATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACgatg                           >  2:1202291/1‑97 (MQ=17)

                                                                       agaTATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGAtgatga                       <  2:222598/100‑1 (MQ=18)

                                                                        gaTATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGaa                      >  2:1735937/1‑100 (MQ=17)

                                                                        gaTATTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGaa                      >  2:774261/1‑100 (MQ=18)

                                                                          tatTTGCTTTTTAAAAATCTTGAACAAGCTGAAAATTGAAATATTTAACATTGAGAGTTGTTCGTGTGTTTTTCTAATTTTCGCAACACGATGATGAATc                    <  2:1863402/100‑1 (MQ=11)

                                                                           atTTGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCg                   >  2:688657/1‑100 (MQ=18)

                                                                              tgatatttAAAAATCTGGAACAAGCTGAAAATTGAAACACTGCACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaa                <  1:704946/95‑1 (MQ=11)

                                                                              tGCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaa                >  2:1776569/1‑100 (MQ=18)

                                                                               gCTCTTTAAAAATCTGGAACAAGCTGAAAATTGCAACACTGAACAAAGAGAGTTGTTCGTGATTCCCTCAACTTTTCGCAACACGATGATGAATCgaaa                >  2:1182387/1‑99 (MQ=11)

                                                                               gCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaag               >  2:2315639/1‑100 (MQ=18)

                                                                               gCTCTTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaag               <  1:1775182/100‑1 (MQ=17)

                                                                                ctctTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaaga              >  2:357619/1‑100 (MQ=17)

                                                                                ctctTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaaga              >  2:510812/1‑100 (MQ=17)

                                                                                  ctgtAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaagaaa            <  1:487441/97‑1 (MQ=16)

                                                                                  ctTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaagaaa            >  2:866013/1‑100 (MQ=17)

                                                                                  ctTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCgaaagaaa            <  2:70717/100‑1 (MQ=18)

                                                                                  ctTTAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTAGCAACACGATGATGAATCgaaagaaa            >  2:478347/1‑100 (MQ=255)

                                                                                   tttAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAATGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAAc           <  2:1678417/100‑1 (MQ=16)

                                                                                   tttAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAAc           >  1:1555447/1‑100 (MQ=18)

                                                                                    ttAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACa          <  2:923787/100‑1 (MQ=18)

                                                                                    ttAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACa          >  2:333623/1‑100 (MQ=17)

                                                                                    ttAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACa          >  1:1303445/1‑100 (MQ=17)

                                                                                    ttAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACa          >  1:1895465/1‑100 (MQ=17)

                                                                                    ttAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACa          <  1:2236083/100‑1 (MQ=17)

                                                                                     tAAAAATCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACAt         >  1:2321681/1‑100 (MQ=18)

                                                                                       aaaaTCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCt       >  1:2236725/1‑100 (MQ=18)

                                                                                       aaaaTCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCt       >  1:506001/1‑100 (MQ=18)

                                                                                          aTCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCg    >  1:2320096/1‑100 (MQ=18)

                                                                                          aTCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCg    >  2:2386350/1‑100 (MQ=18)

                                                                                          aTCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCg    >  1:906838/1‑100 (MQ=18)

                                                                                          aTCTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCg    >  2:237350/1‑100 (MQ=17)

                                                                                            cTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCggg  <  1:1533674/100‑1 (MQ=18)

                                                                                            cTGGAACAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCggg  <  1:77978/100‑1 (MQ=18)

CGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAGAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGAAAGAAACATCTTCGGG  >  NC_000913/4168443‑4168634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`