Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,177,171 T→A 16.2% intergenic (+43/‑187) tufB → / → secE translation elongation factor EF‑Tu 2/preprotein translocase membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,177,1710TA16.2% 274.7 / 35.3 108intergenic (+43/‑187)tufB/secEtranslation elongation factor EF‑Tu 2/preprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (52/38);  new base A (7/11);  total (59/49)
Fisher's exact test for biased strand distribution p-value = 1.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

ATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATA  >  NC_000913/4177072‑4177269
                                                                                                   |                                                                                                  
aTCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGcatcat                                                                                                    >  2:303847/1‑100 (MQ=255)
 tCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAtt                                                                                                   <  2:719667/100‑1 (MQ=255)
  ccGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAttt                                                                                                  <  2:1828266/100‑1 (MQ=255)
   cGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAtt                                                                                                   <  2:1649347/98‑1 (MQ=255)
   cGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTg                                                                                                 <  1:2094084/100‑1 (MQ=255)
   cGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTg                                                                                                 >  2:2047464/1‑100 (MQ=255)
   cGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTg                                                                                                 >  1:1598729/1‑100 (MQ=255)
     tGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGAt                                                                                               >  2:1269398/1‑100 (MQ=255)
     tGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGAt                                                                                               <  1:1000653/100‑1 (MQ=255)
     tGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGAt                                                                                               <  2:157857/100‑1 (MQ=255)
     tGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGAt                                                                                               <  1:1886818/100‑1 (MQ=255)
          gcggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCt                                                                                          <  1:1279769/100‑1 (MQ=255)
          gcggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCt                                                                                          <  1:1237303/100‑1 (MQ=255)
          gcggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCt                                                                                          <  2:913238/100‑1 (MQ=255)
           cggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCtt                                                                                         <  2:434025/100‑1 (MQ=255)
            ggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCttt                                                                                        <  2:643680/100‑1 (MQ=255)
            ggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCttt                                                                                        >  2:277517/1‑100 (MQ=255)
            ggcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCttt                                                                                        <  2:931656/100‑1 (MQ=255)
             gcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCtttt                                                                                       <  2:244076/100‑1 (MQ=255)
             gcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCtt                                                                                         <  2:127267/98‑1 (MQ=255)
              ccGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCttttt                                                                                      >  1:510447/1‑100 (MQ=255)
               cGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTg                                                                                     <  1:656918/100‑1 (MQ=255)
               cGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCttttag                                                                                     <  2:2124382/100‑3 (MQ=255)
                 tACCGTTGGCGGGGGCGGTGTGGCAAAAGTTCTGAGCTAATTGCGGATAAGATTTGACGCCAGGGGCACTATCATGTCATCAAATGATGCCCtatt                                                                                       <  2:475268/96‑1 (MQ=255)
                 tACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCa                                                                                   >  2:2022767/1‑100 (MQ=255)
                 tACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCa                                                                                   <  1:1907811/100‑1 (MQ=255)
                 tACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCa                                                                                   >  2:2261253/1‑100 (MQ=255)
                 tACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCttttagtg                                                                                   <  1:2265906/100‑5 (MQ=38)
                 tACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACAAAAAGGGCATCAAATGATGCCCttttagtg                                                                                   <  2:437295/100‑5 (MQ=38)
                  aCCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCAc                                                                                  <  1:1210895/100‑1 (MQ=255)
                   ccGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACg                                                                                 >  1:2063477/1‑100 (MQ=255)
                   ccGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACg                                                                                 <  2:647803/100‑1 (MQ=255)
                     gTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCaagat                                                                               >  1:2131671/1‑96 (MQ=255)
                     gTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACg                                                                                 >  1:1990734/1‑98 (MQ=255)
                        ggCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTAACGCAATGCGCACTAAAAGGGCAACATTTGATGCCCTTTTTGCACGCttt                                                                             >  2:234939/1‑99 (MQ=255)
                          cgcgGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGt                                                                          >  2:1010346/1‑100 (MQ=255)
                                   tGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAAcc                                                                 <  2:1990712/100‑1 (MQ=255)
                                   tGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAAcc                                                                 <  1:214083/100‑1 (MQ=255)
                                    gTAGAAAAGGTACTGAGCAAATTGCCGATAACATTTGGCGCAATGCGCACTAATAGGACATCATTTGATGCGCTTTTTGTACGCTTTCGTACCAGAAct                                                                 <  2:1839706/99‑2 (MQ=255)
                                     tAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTg                                                               >  2:1817917/1‑100 (MQ=255)
                                      aGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTgg                                                              >  1:954345/1‑100 (MQ=255)
                                       gCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGc                                                             >  2:1195214/1‑100 (MQ=255)
                                       gCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGc                                                             <  2:1993120/100‑1 (MQ=255)
                                        cAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCt                                                            >  1:1797692/1‑100 (MQ=255)
                                          aaaGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCtca                                                          <  2:890554/100‑1 (MQ=255)
                                            aGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCtcatc                                                        <  1:1156947/100‑1 (MQ=255)
                                            aGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCtcatc                                                        <  1:2022789/100‑1 (MQ=255)
                                            aGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCtcagc                                                        <  2:336390/100‑3 (MQ=255)
                                             gTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCtcatca                                                       >  1:403517/1‑100 (MQ=255)
                                              ttCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAg                                                      <  2:810869/100‑1 (MQ=255)
                                                 tGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGa                                                   >  2:817788/1‑100 (MQ=255)
                                                 tGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGa                                                   >  1:2037636/1‑100 (MQ=255)
                                                  gAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGAt                                                  >  2:2137602/1‑100 (MQ=255)
                                                   aGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGAtt                                                 >  2:1758279/1‑100 (MQ=255)
                                                    gCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGAttt                                                >  2:817590/1‑100 (MQ=255)
                                                      tAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTc                                              >  1:1350052/1‑100 (MQ=255)
                                                          tGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTg                                          <  2:1776461/100‑1 (MQ=255)
                                                            ccGATAACATTTGACGCAATGCGCACTAAAAGGGCATAATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTc                                        >  2:1908820/1‑100 (MQ=255)
                                                              gATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCAt                                      >  1:1920302/1‑100 (MQ=255)
                                                                 aaCATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAAt                                   <  1:2022238/100‑1 (MQ=255)
                                                                  aCATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATc                                  >  2:1276495/1‑100 (MQ=255)
                                                                  aCATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATc                                  >  1:2400674/1‑100 (MQ=255)
                                                                   cATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCa                                 >  2:2147068/1‑100 (MQ=255)
                                                                    aTTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCAt                                >  2:2216307/1‑100 (MQ=255)
                                                                     tttGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCAtt                               >  1:492452/1‑100 (MQ=255)
                                                                       tGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTGTGCATGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGc                             <  2:822839/100‑1 (MQ=255)
                                                                        gACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTTTCATAATCATTGc                             >  1:657699/1‑99 (MQ=255)
                                                                        gACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCt                            >  2:1785941/1‑100 (MQ=255)
                                                                        gACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCt                            >  1:686913/1‑100 (MQ=255)
                                                                         aCGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTg                           <  1:1344889/100‑1 (MQ=255)
                                                                          cGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTga                          >  1:1050801/1‑100 (MQ=255)
                                                                          cGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATAATTGCTga                          >  1:1861369/1‑100 (MQ=255)
                                                                            cAATGCGCCCTACAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTTCTgaga                        >  2:521397/1‑100 (MQ=255)
                                                                            cAATGCGCACTAAAAGGGCAACATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTgaga                        >  2:1464951/1‑100 (MQ=255)
                                                                             aaTGCGCACTAAAAGGGAATCAAATGATGCCCTTTTTGCACGATTTGGTACCAGAACCTAGCTCATCCGTGATTTTCTTTGTCATAATAATTGCTgag                         >  2:822239/1‑98 (MQ=255)
                                                                                gcgcACTAAAGTGGCACCATGTGATGCCCTTTTTGCACACTTTCGTACCAGAACGTGGCTCATCCTTGGTGTTCTATCGCACACTCATTGCt                            >  2:1468535/1‑92 (MQ=255)
                                                                                 cgtgcaaaaaGGGCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGCTTTTCTTTGTCATAATCATTGCTGAGACAgg                    >  1:1909118/7‑99 (MQ=37)
                                                                                 cgtgcaaaaaGGGCATCAAATGATGCCCTATTAGTGCGCTTTCGTACCACAACCTGGCTCATCAGTGATGTTCTTTGTCATAATAATTGCTGAGACAGGc                   >  2:962991/7‑100 (MQ=21)
                                                                                 cgcACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAg                     >  1:621021/1‑98 (MQ=255)
                                                                                 cgcACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGc                   <  2:403514/100‑1 (MQ=255)
                                                                                 cgcACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGc                   >  1:1557427/1‑100 (MQ=255)
                                                                                  gcACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGct                  >  1:1522756/1‑100 (MQ=255)
                                                                                   tgcaaaaaGGGCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGctc                 >  2:1892022/5‑100 (MQ=38)
                                                                                    aCTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGctct                <  1:1683501/100‑1 (MQ=255)
                                                                                     caaaaaGGGCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGCTTTTCTTTGTCATAATCATTGCTGAGCCAGGc                   >  2:1868114/3‑96 (MQ=38)
                                                                                     caaaaaGGGCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTg               >  1:643397/3‑100 (MQ=255)
                                                                                      tAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGt              >  2:624888/1‑100 (MQ=255)
                                                                                         aaGGGCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGa           >  2:1882809/1‑100 (MQ=255)
                                                                                          aGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCATAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAg          >  1:2154817/1‑100 (MQ=255)
                                                                                          aGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACTTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGTTGAGACAGGCTCTGTTGAg          <  2:2144506/100‑1 (MQ=255)
                                                                                           gggCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAgg         >  2:628907/1‑100 (MQ=255)
                                                                                           gggCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAgg         <  2:1522859/100‑1 (MQ=255)
                                                                                           gggCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAgg         <  1:2070416/100‑1 (MQ=255)
                                                                                            ggCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAggg        <  2:1855136/100‑1 (MQ=255)
                                                                                             gCATCATTTGATGCCCTTTTTGCACACTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTCTCATAATCATTGCTCACACCCGCTCTGTTAAgcg        >  2:904528/1‑97 (MQ=255)
                                                                                              catcatTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCg      <  1:2327024/100‑1 (MQ=255)
                                                                                              catcatTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCg      >  1:1760790/1‑100 (MQ=255)
                                                                                              catcatTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCg      <  1:2047486/100‑1 (MQ=255)
                                                                                                tcatTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGta    <  2:510444/100‑1 (MQ=255)
                                                                                                  atTTGATGCCCTTTTTGCACGCTTTCATACAAGAACCTGGCTCATCAGTAATTTTCTTTGTCATAATCATTGCTGAGACAGGCACGGTAGAGGGCGtata  >  2:1286182/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
ATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATA  >  NC_000913/4177072‑4177269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: