| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 409,322 | G→T | 57.0% | A233E (GCG→GAG) | rdgC ← | nucleoid‑associated ssDNA and dsDNA binding protein; competitive inhibitor of RecA function |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 409,322 | 0 | G | T | 57.0% | 25.6 / 114.3 | 82 | A233E (GCG→GAG) | rdgC | nucleoid‑associated ssDNA and dsDNA binding protein; competitive inhibitor of RecA function |
| Reads supporting (aligned to +/- strand): ref base G (16/19); new base T (18/28); total (35/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01 | |||||||||||
ATCTTCGTTTTGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACGCCGCCATCTTCCAG > NC_000913/409225‑409419 | atCTTCGTTTTGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTcc < 1:1128697/100‑1 (MQ=255) cTTCGTTTTGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAg < 2:216818/100‑1 (MQ=255) cGTTTTGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGttt < 1:1872625/100‑1 (MQ=255) cGTTTTGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGttt < 1:271944/100‑1 (MQ=255) agTTTTGACAGCACAGCTCGACGCAGAACGACAGACGCGTGAGCGAACCATCGTCGCACATCACAAACCGAATGCACCGCTTCCAGTCGAGTCACAGttt > 2:2357426/2‑100 (MQ=255) ttttGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAg < 2:1691661/100‑1 (MQ=255) tttGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAgn < 2:1191870/100‑2 (MQ=255) ttGATCGCTCATCTCGTCGCATAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTc < 1:697690/100‑1 (MQ=255) ttGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTc < 1:1959416/100‑1 (MQ=255) ttGATCGCGCAGCTCGTCACAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTc < 2:548222/100‑1 (MQ=255) tGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTca > 1:1176407/1‑100 (MQ=255) gATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTcac > 1:2324781/1‑100 (MQ=255) tCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTcacca < 2:203494/100‑1 (MQ=255) gcgcAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACt < 1:2182548/100‑1 (MQ=255) gCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTcc < 1:2239675/100‑1 (MQ=255) gCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTcc < 2:1762413/100‑1 (MQ=255) cTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCg < 1:644200/100‑1 (MQ=255) cgCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTc < 1:1118768/100‑1 (MQ=255) gCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCa > 2:1982495/1‑100 (MQ=255) gCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCa < 1:427088/100‑1 (MQ=255) gAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAAtg < 1:672521/100‑1 (MQ=255) aCTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAAtgtg > 2:1067443/1‑100 (MQ=255) aCTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAAtgtg < 2:184963/100‑1 (MQ=255) aCTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAAtgtg < 2:402969/100‑1 (MQ=255) aCTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAAtgtg > 1:1090992/1‑100 (MQ=255) ttCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGAt < 1:2244805/100‑1 (MQ=255) ttCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGAt < 2:899254/100‑1 (MQ=255) aCGTTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTg < 1:611065/100‑1 (MQ=255) aCGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTg < 2:910146/100‑1 (MQ=255) cGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGa > 2:388832/1‑100 (MQ=255) cGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGa < 1:127499/100‑1 (MQ=255) cGCTTGAGCGAACCATCGTAGCACATCACAAACTGAATGCGCTGCTGCCAGACGAGCGACAGTTTAGTCACCACTTTTCCGGATTCAACGTCATTGGTg > 2:2290985/1‑99 (MQ=255) cTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGAtc < 1:2203188/100‑1 (MQ=255) cTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGAtc < 2:1041109/100‑1 (MQ=255) cTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGAtc > 2:334491/1‑100 (MQ=255) cTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGAtc < 2:1077476/100‑1 (MQ=255) cTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGAtc > 1:1702824/1‑100 (MQ=255) gAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATctct > 1:1601797/1‑100 (MQ=255) cGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCg < 1:1847481/100‑1 (MQ=255) gAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGc < 1:582434/100‑1 (MQ=255) aCCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTg < 1:111182/100‑1 (MQ=255) aCCATCGTCGCACATCACAAACTGAATGCACTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTg < 2:122083/100‑1 (MQ=255) ccATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTgg < 2:188919/100‑1 (MQ=255) ccATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTgg < 1:334494/100‑1 (MQ=255) tcgtcgCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCa < 2:1406668/100‑1 (MQ=255) gtcgCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGa < 2:1470485/100‑1 (MQ=255) cgCACATCACAAACTGAATGCGCTGCAGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGCGATTGGTGAACTCTTCGCTGGTCAGATc > 2:741118/1‑100 (MQ=255) gCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCt > 1:2171058/1‑100 (MQ=255) cacaTCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCtt > 2:1665937/1‑100 (MQ=255) cacaTCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGGCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTgg < 2:2194036/91‑1 (MQ=255) acaTCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTGTTAGCTGGTCAGATCTTg > 2:363258/1‑100 (MQ=255) caTCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGt < 2:247242/100‑1 (MQ=255) caTCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGt < 2:1090982/100‑1 (MQ=255) tCACAAACTGAATGCGCTGCTGTCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGttt > 2:1643772/1‑100 (MQ=255) cacaAACTGAATGCGCTGCTGCCAGTCGAGCTCAAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGCTGATCTCTTCGCTGGTCAGATCTTGTttc > 2:1439843/1‑100 (MQ=255) cacaAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTttc < 1:1373030/100‑1 (MQ=255) acaAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTttct > 1:386769/1‑100 (MQ=255) caAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTttctt > 1:134080/1‑100 (MQ=255) caAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTttctt > 2:805274/1‑100 (MQ=255) aaaCTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTttcttc > 1:122635/1‑100 (MQ=255) aaCTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGTCTTCAATGTTATTGGTGATCTCTTCGCTGGTTAGATCTTGTTTCTTcg < 2:709303/100‑1 (MQ=255) aCTGAATGCGCCGCTGCCACTCCAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGTTTTCTTATCTCTTCACTGGTCACATCTTGTTTCTTcg < 2:1392439/99‑1 (MQ=255) cTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTcgcg > 1:1438636/1‑100 (MQ=255) gAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTcgcgcg > 1:1969670/1‑100 (MQ=255) gAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTcgcgc > 1:616300/1‑99 (MQ=255) aTGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGa < 1:726115/100‑1 (MQ=255) gcgcTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATc < 1:1797387/100‑1 (MQ=255) gcgcTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATc < 1:1838279/100‑1 (MQ=255) gcgcTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATc > 2:1040007/1‑100 (MQ=255) gctgctGCCAGTCGAGCTCAAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCAc > 2:329589/1‑100 (MQ=255) gctgctGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCAc > 2:1542539/1‑100 (MQ=255) gctgcCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCAcgcc > 1:972052/1‑100 (MQ=255) ctgcCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCAcgcc > 2:1885683/1‑99 (MQ=255) ctgcCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCAcgccg < 2:2023573/100‑1 (MQ=255) tgcCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCAcgccgc < 1:1954627/100‑1 (MQ=255) tgcCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCAcgccgc > 1:389288/1‑100 (MQ=255) gcCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACgccgcc > 2:71719/1‑100 (MQ=255) cAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACGCCGCCAt < 2:972041/100‑1 (MQ=255) aGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACGCCGCCATc > 1:1197945/1‑100 (MQ=255) tCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACGCCGCCATCtt > 2:2144628/1‑100 (MQ=255) tCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACGCCGCCATCtt > 2:2144272/1‑100 (MQ=255) gAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACGCCGCCATCTTcc > 2:2121388/1‑100 (MQ=255) gcgcCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACGCCGCCATCTTCCAg < 1:1062311/100‑1 (MQ=255) | ATCTTCGTTTTGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACGCCGCCATCTTCCAG > NC_000913/409225‑409419 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |