Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,279,370 G→T 23.2% A298S (GCA→TCA)  ghxP → guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,279,3700GT23.2% 288.0 / 84.5 138A298S (GCA→TCA) ghxPguanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base G (53/53);  new base T (16/16);  total (69/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.64e-01

Only 100 of 140 total aligned reads displayed.

GTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCG  >  NC_000913/4279271‑4279469
                                                                                                   |                                                                                                   
 tgatgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGc                                                                                                    <  2:2284017/100‑1 (MQ=255)
 tgatgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGc                                                                                                    <  1:732966/100‑1 (MQ=255)
  gatgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATca                                                                                                   <  1:628710/100‑1 (MQ=255)
    tgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcact                                                                                                 <  2:607667/100‑1 (MQ=255)
     gaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactg                                                                                                <  1:1127804/100‑1 (MQ=255)
      agcgCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactga                                                                                               <  2:444710/97‑1 (MQ=255)
       cGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGTGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgac                                                                                              >  1:2045888/1‑100 (MQ=255)
       cGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgac                                                                                              >  1:1123082/1‑100 (MQ=255)
       cGGAAGTATTTGATGCCACCGGAACTATCCGTCCCGTCGCAGGCCAGGCGAACCTGCTGGATAAAGATAGGCCGCTCATCCACCATGGGAAAGcac                                                                                                  >  2:2275367/1‑96 (MQ=255)
         gCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCCTAGACGGTGGGAAATcactgacca                                                                                            >  2:1925398/1‑100 (MQ=255)
          cAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGTCAGATCATCGACGGTGGGAAAGcactgaccac                                                                                           <  1:1169917/100‑1 (MQ=255)
          cAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgaccac                                                                                           >  1:290885/1‑100 (MQ=255)
            gTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGCTGGGCAGATCATCGACGGTGGGAAAGcactgcccactg                                                                                         >  1:2033203/1‑100 (MQ=255)
            gTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgaccactg                                                                                         <  1:1561595/100‑1 (MQ=255)
            gTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgaccactg                                                                                         <  1:1775274/100‑1 (MQ=255)
            gTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgaccactg                                                                                         >  2:1751715/1‑100 (MQ=255)
            gTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgaccact                                                                                          >  1:1603988/1‑99 (MQ=255)
            gTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGcactgaccac                                                                                           >  1:2222859/1‑98 (MQ=255)
                ttGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTc                                                                                     >  1:560420/1‑100 (MQ=255)
                 tGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTcc                                                                                    >  1:1300592/1‑100 (MQ=255)
                  gATGCCACCGGAACTATCCGTGCCGTGGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCa                                                                                   >  1:1454669/1‑100 (MQ=255)
                    tcccACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATg                                                                                 <  1:1236213/98‑1 (MQ=255)
                    tGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATg                                                                                 <  1:1803632/100‑1 (MQ=255)
                     gCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATg                                                                                 <  1:1155405/99‑1 (MQ=255)
                     gCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGa                                                                                <  2:1544906/100‑1 (MQ=255)
                     gCCACCGGAACTATCCGTGCAGTCGCAGGCCAGGAGAACCTGCTGGATAAAGATGAGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTc                                                                                     >  2:404928/1‑95 (MQ=255)
                       cACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGGCTCCATGag                                                                               <  2:1218188/99‑1 (MQ=255)
                       cACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGagc                                                                              >  1:1201890/1‑100 (MQ=255)
                       cACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGagc                                                                              >  2:331537/1‑100 (MQ=255)
                       cACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGagc                                                                              <  1:1520814/100‑1 (MQ=255)
                       cACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGagc                                                                              <  1:1432453/100‑1 (MQ=255)
                         ccGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGagcag                                                                            >  2:1748650/1‑100 (MQ=255)
                            gAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGt                                                                         >  2:25780/1‑100 (MQ=255)
                            gAACTATCCGTGCCGTCGAAGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGt                                                                         >  2:301659/1‑100 (MQ=255)
                             aaCTATCCGTGCCGTCGCCGGCCAGTCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGtt                                                                        <  2:1509956/100‑1 (MQ=255)
                               cTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGttt                                                                       <  1:608707/99‑1 (MQ=255)
                                tATCCTTGCCGTCGCCGGCCAGGCGAACCTGCTGGATACAGATGGGCAAATCATCAACGGTGGGGCAGCTCTGACCACTGTCTTCATGACCAGCGTTTtc                                                                     <  2:1845643/100‑1 (MQ=255)
                                 aTCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTtct                                                                    >  1:1430586/1‑100 (MQ=255)
                                   ccGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTctct                                                                  >  1:906129/1‑100 (MQ=255)
                                   cagcaggttCGCCTGGCCGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTctct                                                                  >  2:1139097/9‑100 (MQ=255)
                                    cGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTTCATGAGCAGCGTTTTCGCTg                                                                 <  2:96718/100‑1 (MQ=255)
                                    cGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTg                                                                 >  2:1275601/1‑100 (MQ=255)
                                     gTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTgg                                                                <  2:2398770/100‑1 (MQ=255)
                                       gCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGcc                                                              <  2:1300578/100‑1 (MQ=255)
                                       gCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGcc                                                              <  1:2065782/100‑1 (MQ=255)
                                        ccGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCt                                                             >  1:246114/1‑100 (MQ=255)
                                        ccGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCt                                                             <  1:2175904/100‑1 (MQ=255)
                                        ccGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCt                                                             >  1:45463/1‑100 (MQ=255)
                                          gTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtgg                                                           <  1:955650/100‑1 (MQ=255)
                                          gTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtgg                                                           <  2:977192/100‑1 (MQ=255)
                                           tCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtggt                                                          <  2:216718/100‑1 (MQ=255)
                                           tCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtggt                                                          <  1:2303488/100‑1 (MQ=255)
                                             gCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGc                                                               >  1:1977606/1‑93 (MQ=255)
                                              ccGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTggtggg                                                       <  2:2137950/100‑1 (MQ=255)
                                              ccGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTggtggg                                                       >  2:2335162/1‑100 (MQ=255)
                                               cGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTggtgggt                                                      <  1:985662/100‑1 (MQ=255)
                                               cGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTggtgggt                                                      >  2:1186726/1‑100 (MQ=255)
                                                ggCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTggtgggtg                                                     >  2:835463/1‑100 (MQ=255)
                                                ggCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTggtgggtg                                                     <  1:2126832/100‑1 (MQ=255)
                                                 gCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGc                                                    >  2:2047301/1‑100 (MQ=255)
                                                  ccAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCg                                                   <  2:1388843/100‑1 (MQ=255)
                                                    aGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGc                                                 >  2:788876/1‑100 (MQ=255)
                                                    aGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGc                                                 <  2:2330477/100‑1 (MQ=255)
                                                     ggCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCt                                                >  1:269292/1‑100 (MQ=255)
                                                       ngAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGGGGGTGCGGCTcc                                              >  2:2384613/2‑100 (MQ=255)
                                                        gacccTGCTGGATAAAGATGGGCAGATCATTGACGGTGGGAAATCACTGACCACGGACTCCATGTGCAGCGTTTTCTCTGGCCTGATGGGTGCGGCTCCg                                             <  2:2300670/97‑1 (MQ=255)
                                                        gAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTccn                                             <  1:1915287/100‑2 (MQ=255)
                                                          aCCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGc                                           <  2:1301939/100‑1 (MQ=255)
                                                            ctgctgGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAg                                         <  1:278514/100‑1 (MQ=255)
                                                             tgctgGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGc                                        <  1:1632777/100‑1 (MQ=255)
                                                              tctgGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCg                                       <  1:1461680/99‑1 (MQ=255)
                                                              gctgGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCg                                       >  2:656126/1‑100 (MQ=255)
                                                               ctgGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCgg                                      <  1:2133378/100‑1 (MQ=255)
                                                               ctgGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCgg                                      >  1:805529/1‑100 (MQ=255)
                                                               ctgGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCga                                      >  2:1461664/1‑99 (MQ=255)
                                                                tgGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACGACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGt                                     >  2:172258/1‑100 (MQ=255)
                                                                 ggATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGta                                    >  1:1371515/1‑100 (MQ=255)
                                                                      aaGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACAt                               <  1:1518664/100‑1 (MQ=255)
                                                                        gATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCg                             >  1:1744521/1‑100 (MQ=255)
                                                                           gggCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGt                          <  2:493557/100‑1 (MQ=255)
                                                                            ggCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTc                         >  1:891451/1‑100 (MQ=255)
                                                                             gCAGATCATCGTCGGGGGGAAAGCACTGACCACTGACGCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTc                         <  1:858507/99‑1 (MQ=255)
                                                                             gCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCt                        >  1:1403627/1‑100 (MQ=255)
                                                                                gATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcg                     >  1:57850/1‑100 (MQ=255)
                                                                                gATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGATTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcg                     >  2:2029988/1‑100 (MQ=255)
                                                                                  tcatcGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcggc                   <  1:397272/100‑1 (MQ=255)
                                                                                   catcGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcggcg                  >  1:2145882/1‑100 (MQ=255)
                                                                                   catcGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcggcg                  <  2:2211762/100‑1 (MQ=255)
                                                                                         cGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcg            <  1:2338870/100‑1 (MQ=255)
                                                                                           gTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggc          <  1:1174561/100‑1 (MQ=255)
                                                                                           gTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTa              >  1:1421790/1‑96 (MQ=255)
                                                                                           gTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggc          >  1:331331/1‑100 (MQ=255)
                                                                                             gggAAAGCACTGACCACTGACTACATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCACCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcgg        >  2:156954/1‑100 (MQ=255)
                                                                                              ggAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcggn       <  1:2384641/100‑2 (MQ=255)
                                                                                              ggAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcggc       >  1:2369410/1‑100 (MQ=255)
                                                                                               gAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcggcg      >  2:451356/1‑100 (MQ=255)
                                                                                               gAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcggcg      >  2:1240465/1‑100 (MQ=255)
                                                                                                  aGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCggcgggc   <  1:373661/100‑1 (MQ=255)
                                                                                                  aGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCggcggg    >  2:1016392/1‑99 (MQ=255)
                                                                                                   gCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCggcgggcg  >  1:2145682/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
GTGATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCGGGCG  >  NC_000913/4279271‑4279469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: