Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,312,089 A→G 15.0% intergenic (‑47/+12) alsB ← / ← alsR D‑allose ABC transporter periplasmic binding protein/d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,312,0890AG15.0% 336.0 / 49.0 148intergenic (‑47/+12)alsB/alsRD‑allose ABC transporter periplasmic binding protein/d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR
Reads supporting (aligned to +/- strand):  ref base A (62/62);  new base G (9/13);  total (73/75)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.52e-01

Only 100 of 151 total aligned reads displayed.

ACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCG  >  NC_000913/4311991‑4312188
                                                                                                  |                                                                                                   
acaacaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAAt                                                                                                    <  2:250090/100‑1 (MQ=255)
 caacaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAAtt                                                                                                   >  1:1701425/1‑100 (MQ=255)
 caacaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAAtt                                                                                                   >  2:2011324/1‑100 (MQ=255)
 caacaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCAGCGATGGCGGAAtt                                                                                                   >  2:2138019/1‑100 (MQ=255)
 caacaTTAAGCACACGAGTGTGCCGGTGAAATATTTCAGGTATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGaa                                                                                                     <  1:351196/98‑1 (MQ=255)
   acaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTAc                                                                                                 <  1:1074984/100‑1 (MQ=255)
   acaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTAc                                                                                                 >  2:2135228/1‑100 (MQ=255)
    caTTATTCCCACTAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTAcc                                                                                                <  1:1294429/100‑1 (MQ=37)
    caTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTAcc                                                                                                <  2:1786458/100‑1 (MQ=38)
    caTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTAcc                                                                                                <  1:1412274/100‑1 (MQ=38)
    caTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTAcc                                                                                                <  1:1645813/100‑1 (MQ=255)
    caTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTAcc                                                                                                <  2:1511754/100‑1 (MQ=255)
    caTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCCCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTAcc                                                                                                <  2:2070269/100‑1 (MQ=38)
     aTTAAGCCCACTAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTAcc                                                                                                <  2:2150182/99‑1 (MQ=38)
     aTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCt                                                                                               <  2:1524874/100‑1 (MQ=38)
     aTTAAGCCCACGAGTGAGCCGATGAAATATTTCAGATATTTATTCATAATAATGTTGTCACGATAAGGGGACAGGAAACTCCACGATGGCGGAATTa                                                                                                  >  2:241080/1‑97 (MQ=255)
      ttAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtg                                                                                              <  1:513527/100‑2 (MQ=38)
        aaccccACGAGTGTGCCACTAAAATATTTCAAATATTTATTCATAATAATGTTCTCACGGTAAGCGGAACGGTAACTCCGCGATAAAACAATTACCttt                                                                                             >  2:1962111/4‑99 (MQ=255)
        aaGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtgtc                                                                                            <  1:2305928/100‑4 (MQ=37)
        aaGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCtttt                                                                                            >  1:818185/1‑100 (MQ=255)
         aGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCttttt                                                                                           >  1:1607487/1‑100 (MQ=255)
         aGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCttttt                                                                                           >  1:1603209/1‑100 (MQ=255)
          gCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGTGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTc                                                                                          <  1:1690549/100‑1 (MQ=255)
          gCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCttttt                                                                                           >  2:1208607/1‑99 (MQ=255)
          gCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTc                                                                                          >  2:2024141/1‑100 (MQ=255)
          gCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTc                                                                                          >  1:391424/1‑100 (MQ=255)
          gCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTc                                                                                          >  1:2194841/1‑100 (MQ=255)
           cccACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtgtcccc                                                                                         <  1:656943/100‑7 (MQ=25)
             cACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAGGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTa                                                                                       >  2:1678443/1‑100 (MQ=255)
               cGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTAtt                                                                                     <  2:110870/100‑1 (MQ=255)
               cGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTAtt                                                                                     <  1:378417/100‑1 (MQ=255)
               cGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTAtt                                                                                     <  1:457921/100‑1 (MQ=255)
               cGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTAtt                                                                                     >  2:428403/1‑100 (MQ=255)
                 aGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTc                                                                                   >  2:580535/1‑100 (MQ=255)
                  gtgtGCAGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGAGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTc                                                                                   <  2:989490/99‑1 (MQ=255)
                   tgtgCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAg                                                                                 <  2:973260/100‑1 (MQ=255)
                      gCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcgc                                                                              >  1:988218/1‑100 (MQ=255)
                       ccGCTGAAATATTTCAGATATTTATTAATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGACTTACCTTTTTCTTATTTCAgcgcg                                                                             >  2:978550/1‑100 (MQ=255)
                        cGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcgcgc                                                                            >  1:1546116/1‑100 (MQ=255)
                             aaaTATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGTTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGt                                                                       >  2:81831/1‑100 (MQ=255)
                              aaTATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTg                                                                      <  1:1342248/100‑1 (MQ=255)
                              aaTATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTg                                                                      <  1:1427467/100‑1 (MQ=255)
                              aaTATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTg                                                                      <  2:1734675/100‑1 (MQ=255)
                               atatTTCAGATATTTATTCATAATAATGTTCTAACGGTAAGGGGACAAGTAACTACGCGATGGCGGAATTACGTTGTTCTTATTTCAGCGCGCCTg                                                                         >  2:2395906/1‑96 (MQ=255)
                                tatTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTgag                                                                    <  1:1823387/100‑1 (MQ=255)
                                tatTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTgag                                                                    <  2:3013/100‑1 (MQ=255)
                                  tttCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGagaa                                                                  <  1:1478820/100‑1 (MQ=255)
                                  tttCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGagaa                                                                  <  1:1364536/100‑1 (MQ=255)
                                   ttCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGagaag                                                                 <  2:892406/100‑1 (MQ=255)
                                     cAGATATTTATTCATAATAATGTTCTCAAGGTAAGGGGACAGGTAACTACGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAgaagaa                                                               >  2:183456/1‑100 (MQ=255)
                                      aGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGCGAAGaaa                                                              >  1:463847/1‑100 (MQ=255)
                                       gATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAAt                                                             <  1:2233523/100‑1 (MQ=255)
                                         tatttattCATACTAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATcn                                                           <  1:945684/100‑2 (MQ=255)
                                         tatttattCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATca                                                           >  2:862648/1‑100 (MQ=255)
                                         tatttattCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATca                                                           >  1:1251423/1‑100 (MQ=255)
                                           tttattCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCAGAATTACCTTTTGCTTATTTCAGCGCGCCTGTTGAGAAGAAATcaac                                                         >  2:681785/1‑100 (MQ=255)
                                              attCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAAt                                                      >  1:1114944/1‑100 (MQ=255)
                                              attCATAANAATGTTCTCACGGTAAGNGGACNGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATNAACAAt                                                      >  1:2270362/1‑100 (MQ=255)
                                                  ataataATGTTCTCACGGTAAGCAGAAATGTAACTCCGCGATGGGGAAATTACCTATGTCTGAAATTAGCGGGCAAGGTGAAAAGAAATCAATAATTgc                                                   >  2:1072997/1‑99 (MQ=255)
                                                   taataaTGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTgcgc                                                 >  2:73081/1‑100 (MQ=255)
                                                     ataatCTTCACACGGTAAGCGGCTAGGTAACTCCGTGATCGCGGGTTTACGGTTGTCTTATTTCAGCGCGCCTGGTGATAAGGATTCATCAATTCGGCCg                                               >  2:1641036/1‑100 (MQ=255)
                                                            tctcACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGtttttt                                        <  1:662319/100‑1 (MQ=255)
                                                            tctcACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGtttttt                                        >  1:2287476/1‑100 (MQ=255)
                                                             ctcACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTg                                       <  2:2345382/100‑1 (MQ=255)
                                                               cACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGc                                      >  2:2069947/1‑99 (MQ=255)
                                                               cACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGc                                      <  1:1081675/99‑1 (MQ=255)
                                                               cACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCa                                     <  1:104086/100‑1 (MQ=255)
                                                                 cGGTAAGGGGACAGGTAACTCCGCGATAGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGTTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGAata                                   >  2:913655/1‑100 (MQ=255)
                                                                  ggTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatat                                  <  1:2178627/100‑1 (MQ=255)
                                                                     taagaaaaaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTACatattaa                               >  1:799331/9‑100 (MQ=25)
                                                                      atgggACAGGTAACTACGCGATGGCGGAATTAACTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaa                               <  1:1848274/97‑1 (MQ=255)
                                                                      aagaaaaaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaat                              >  2:523957/8‑100 (MQ=37)
                                                                       ggggACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaata                             <  1:572487/100‑1 (MQ=255)
                                                                       ggggACAGGTAACTCCGCGAAGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaata                             >  2:1460449/1‑100 (MQ=255)
                                                                       agaaaaaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaata                             >  1:786035/7‑100 (MQ=25)
                                                                        gtgACAGGTAACGCCGCGATGGCGGAATTACCTTTTTCTGAGTTCAGCGCGCTTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaata                             <  2:2127705/97‑1 (MQ=255)
                                                                          gACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaatatta                          <  2:1662094/100‑1 (MQ=255)
                                                                           aaaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAg                         >  1:1992869/3‑100 (MQ=38)
                                                                           aaaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAg                         >  1:2199542/3‑100 (MQ=38)
                                                                           aCAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAg                         <  2:818176/100‑1 (MQ=255)
                                                                            cAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGc                        <  2:1190006/100‑1 (MQ=255)
                                                                            aaGGTAATTCCGCCATCGCGGAGATACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGc                        >  2:126580/2‑100 (MQ=38)
                                                                              ggTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCtt                      >  1:1013042/1‑100 (MQ=255)
                                                                              ggTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCtt                      >  1:1944978/1‑100 (MQ=255)
                                                                              ggTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCtt                      >  2:1822133/1‑100 (MQ=255)
                                                                               gTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTg                     >  1:295572/1‑100 (MQ=255)
                                                                               gTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTg                     <  2:865783/100‑1 (MQ=255)
                                                                                 aaCTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGtt                   >  1:1626479/1‑100 (MQ=255)
                                                                                  aCTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTc                  <  2:1349095/100‑1 (MQ=255)
                                                                                   cTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCa                 <  1:2110902/100‑1 (MQ=255)
                                                                                    tCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCaa                <  2:1603189/100‑1 (MQ=255)
                                                                                     ccGCGATGGCGGAATTACCTTTTTTTTTTTTTTTCGAGCCTGGTGAGAAGAAATCAAAAATTGCGCGGGTTTTTTTCATATTAATATTAGCTTGTTCAAt               >  2:434988/1‑100 (MQ=255)
                                                                                       gcgATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGt             >  1:1390298/1‑100 (MQ=255)
                                                                                       gcgATGGCAGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGt             <  1:120087/100‑1 (MQ=255)
                                                                                        cgATGGCCGAATTACCCTTTTCTTATTTCAGCCAGCCCGATGAGCAGAAATCAACAACTGCGCAGGATTTTTGCATATTAATATTACATTGATCAATg              >  2:1880562/1‑98 (MQ=255)
                                                                                          aTGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGa          >  1:1124153/1‑100 (MQ=255)
                                                                                          aTGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGa          >  1:1740567/1‑100 (MQ=255)
                                                                                            ggCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTCATATTAGCTTGTTCAATGTTGAg         >  2:352970/1‑99 (MQ=255)
                                                                                             gCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGc        <  1:2086482/99‑1 (MQ=255)
                                                                                               ggAATTACCTTTTTATTATTTCAGCGCGCATGGTGAGAAGAAATCAACATTTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGc        <  2:1945477/97‑1 (MQ=255)
                                                                                                  |                                                                                                   
ACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCG  >  NC_000913/4311991‑4312188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: