Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,324,454 G→T 29.1% Q238K (CAA→AAA)  phnC ← phosphonate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,324,4540GT29.1% 188.2 / 84.1 110Q238K (CAA→AAA) phnCphosphonate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (40/38);  new base T (14/18);  total (54/56)
Fisher's exact test for biased strand distribution p-value = 5.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01

Only 100 of 112 total aligned reads displayed.

GTTTTCCTCAATGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAGCGTGACGACCAC  >  NC_000913/4324355‑4324550
                                                                                                   |                                                                                                
gTTTTCCTCAATGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTg                                                                                                  <  2:1010747/100‑1 (MQ=255)
  tttCCTCAATGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGgc                                                                                                <  1:80755/100‑1 (MQ=255)
    tCCTCAATGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGgctg                                                                                              >  1:815845/1‑100 (MQ=255)
     ccTCAATGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTtgctgc                                                                                             >  1:2077180/1‑100 (MQ=255)
     ccTCAATGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGgctgc                                                                                             >  2:1178439/1‑100 (MQ=255)
     ccTCAATGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGgctgc                                                                                             >  2:2303199/1‑100 (MQ=255)
      cTCAATGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTgctgct                                                                                            >  1:810699/1‑100 (MQ=255)
      cTCAATGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGgctgct                                                                                            >  1:584993/1‑100 (MQ=255)
      cTCAATGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGgctgct                                                                                            >  1:1950862/1‑100 (MQ=255)
        cAATGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCtgctgc                                                                                          >  2:981663/1‑100 (MQ=255)
        cAATGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCtgctgc                                                                                          <  2:417482/100‑1 (MQ=255)
         aaTGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGcc                                                                                         >  2:406914/1‑100 (MQ=255)
          atgatgGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCcg                                                                                        >  2:1585133/1‑100 (MQ=255)
            gatgGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCcgtc                                                                                      <  2:1340010/100‑1 (MQ=255)
            gatgGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCcgtc                                                                                      <  1:415261/100‑1 (MQ=255)
            gatgGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCcgtc                                                                                      >  2:740642/1‑100 (MQ=255)
             atgGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCcgtcg                                                                                     >  1:239077/1‑100 (MQ=255)
             atgGGGATGTCAGGCAGCTTTCGCGTTCGCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCcgtcg                                                                                     <  1:470400/100‑1 (MQ=255)
              tgGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCcgtcgt                                                                                    <  2:1566108/100‑1 (MQ=255)
               ggggATGTTAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTGGAGATGGTCAAAACGTTCGTTATTAAACTGTTTGCTGCTGCCGTCGTa                                                                                   <  1:1432491/100‑1 (MQ=255)
               ggggATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTa                                                                                   <  2:939064/100‑1 (MQ=255)
               ggggATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCGGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTa                                                                                   >  1:2286732/1‑100 (MQ=255)
                 ggATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTaga                                                                                 <  2:1105199/100‑1 (MQ=255)
                  gATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTag                                                                                  <  1:2003678/98‑1 (MQ=255)
                   aTGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCATCGTagaag                                                                               <  1:1278547/100‑1 (MQ=255)
                    tGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTagaaga                                                                              <  1:829919/100‑1 (MQ=255)
                     gTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGAc                                                                             >  2:43494/1‑100 (MQ=255)
                       cAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGt                                                                           >  1:1590948/1‑100 (MQ=255)
                         ggCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGc                                                                         <  1:1113266/100‑1 (MQ=255)
                           cAGCTTTCGCGTTCTCTTCGACGAGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGcc                                                                        <  1:1992490/99‑1 (MQ=255)
                            aGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGcccc                                                                      <  1:1290197/100‑1 (MQ=255)
                              cTTTCGCGTTCTCTTCGACGCGGTTAATGGTGCGCTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTg                                                                    <  1:1639756/100‑1 (MQ=255)
                              cTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTg                                                                    >  2:243642/1‑100 (MQ=255)
                                  cgcgTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGgcgc                                                                >  1:339544/1‑100 (MQ=255)
                                   gcgTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCa                                                               >  1:2391152/1‑100 (MQ=255)
                                    cgTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAg                                                              >  2:218292/1‑100 (MQ=255)
                                    cgTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGccccagatcgga                                                              >  1:989321/1‑92 (MQ=255)
                                     gTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAgg                                                             <  1:2097980/100‑1 (MQ=255)
                                      ttCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAggg                                                            >  1:32021/1‑100 (MQ=255)
                                       tctcTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGc                                                           >  2:940625/1‑100 (MQ=255)
                                        ctctTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGcg                                                          <  2:2077158/100‑1 (MQ=255)
                                           ttCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGcgacg                                                       <  1:1942607/100‑1 (MQ=255)
                                            tCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGcgacga                                                      >  1:2213322/1‑100 (MQ=255)
                                            tCGACGCGGTTAATGCTGCAGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGcgacg                                                       >  2:201125/1‑99 (MQ=255)
                                              gaatcgGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGTCGACGATg                                                    <  1:531252/96‑1 (MQ=255)
                                              gACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATg                                                    <  1:954755/100‑1 (MQ=255)
                                               aCGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGc                                                   <  2:239074/100‑1 (MQ=255)
                                                  cgGTTAATGCTGCGGTAGAGCTGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTCGAAGACGTGCCCCTGGCGCAGGGCGACGATGCCtt                                                <  2:2005513/100‑1 (MQ=255)
                                                   ggTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCAGGCGCAGGGCCACGATACg                                                  >  1:745975/1‑97 (MQ=255)
                                                    gTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGGTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGtt                                                <  1:1686540/98‑1 (MQ=255)
                                                      tAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTAGc                                             >  2:2185507/1‑99 (MQ=255)
                                                       aaTGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAg                                           <  1:1984650/100‑1 (MQ=255)
                                                        aTGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGt                                          <  1:762535/100‑1 (MQ=255)
                                                        aTGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAg                                           >  2:821731/1‑99 (MQ=255)
                                                        aTGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGt                                          >  2:146928/1‑100 (MQ=255)
                                                        aTGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGt                                          <  2:22958/100‑1 (MQ=255)
                                                        aTGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGt                                          <  1:20015/100‑1 (MQ=255)
                                                        aTGCTGAGGTTAAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTTCCCCTGGCGAAGGGCGACGATGCGTTCGCAGt                                          <  2:850577/100‑1 (MQ=255)
                                                          gcttcGTTAGAGATGGTCAAAACGTTCGTTATCAAGCTGTTGGCTGCTGCCGTGGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAg                                        <  2:1830607/100‑1 (MQ=255)
                                                          gctgcGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAg                                        >  1:2140652/1‑100 (MQ=255)
                                                            tgcGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAgcg                                      >  1:1777997/1‑100 (MQ=255)
                                                               ggTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGTGCGACGATGCGTTCGCAGTAGCGCAg                                   <  2:1027609/100‑1 (MQ=255)
                                                               ggTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAg                                   >  1:1600275/1‑100 (MQ=255)
                                                               ggTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAg                                   >  1:2358381/1‑100 (MQ=255)
                                                               ggTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAg                                   <  2:1211160/100‑1 (MQ=255)
                                                                gTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAg                                   <  2:454026/99‑1 (MQ=255)
                                                                 tAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAggg                                 <  1:511744/100‑1 (MQ=255)
                                                                 tAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAggg                                 <  2:499068/100‑1 (MQ=255)
                                                                  agagATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGc                                <  2:247404/100‑1 (MQ=255)
                                                                    agaTGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGt                              <  1:146931/100‑1 (MQ=255)
                                                                     gaTGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTa                             <  2:1857173/100‑1 (MQ=255)
                                                                     gaTGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTa                             >  1:43829/1‑100 (MQ=255)
                                                                     gaTGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTa                             <  1:159485/100‑1 (MQ=255)
                                                                      aTGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTaa                            <  2:151175/100‑1 (MQ=255)
                                                                       tGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAAt                           <  1:1815091/100‑1 (MQ=255)
                                                                        ggTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGCTGCGTTCGCAGTAGCGCCGGGcagatcg                          >  1:2190411/1‑94 (MQ=255)
                                                                        ggTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATc                          <  2:32020/100‑1 (MQ=255)
                                                                                cgttcgttATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGAt                  >  1:2210621/1‑100 (MQ=255)
                                                                                cgttcgttATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGAt                  <  1:144050/100‑1 (MQ=255)
                                                                                cgttcgttATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGAt                  >  2:1787495/1‑100 (MQ=255)
                                                                                cgttcgttATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGAt                  >  1:1927685/1‑100 (MQ=255)
                                                                                  ttcgttATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATg                 >  1:637261/1‑99 (MQ=255)
                                                                                   tcgttATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCa               <  2:810690/100‑1 (MQ=255)
                                                                                     gttATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAGc             >  2:517894/1‑100 (MQ=255)
                                                                                     gttATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAGc             >  2:498697/1‑100 (MQ=255)
                                                                                      ttATCAACCTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCATGGCGCAGGGTGACGATGCGTTGGCAGTAGCGGCGGGCGTGATCCACCTgtggaggcg            >  2:487570/1‑92 (MQ=255)
                                                                                       tATCGAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAGCGt           <  2:1137460/100‑1 (MQ=255)
                                                                                        agcAAACTGTTTGCTGCTGCCTTCGTAGAAGACGTGTCCCTTGCGCAGGGCGACGATGCGTTCGCAGTAGCGGAGTTCTTAATCCACCTGATGCAg              <  2:251101/94‑1 (MQ=255)
                                                                                        aTCGAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAGCGTg          <  2:1797913/100‑1 (MQ=255)
                                                                                        aTCAAACTTTTGGCTGCTGCCGTCTTAGAAGACGTGGCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAGCGTg          <  1:1034576/100‑1 (MQ=255)
                                                                                        aTCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAGCGTg          >  2:183554/1‑100 (MQ=255)
                                                                                        aTCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAGCGTg          <  2:819869/100‑1 (MQ=255)
                                                                                          taaaCTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAGCGTgac        <  1:462521/99‑1 (MQ=255)
                                                                                          cAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAGCGTgac        >  1:2143545/1‑100 (MQ=255)
                                                                                           aaaCTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAGCGTgacg       >  2:810256/1‑100 (MQ=255)
                                                                                           aaaCTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAGCGTgaca       >  2:462518/1‑99 (MQ=255)
                                                                                              cTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAGCGTGACGAcc    >  1:1341846/1‑100 (MQ=255)
                                                                                              cTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAGCGTGACGAcc    <  1:1178450/100‑1 (MQ=255)
                                                                                               tGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAGCGTGACGACCa   >  2:1001618/1‑100 (MQ=255)
                                                                                                gTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAGCGTGACGACCAc  >  2:1117831/1‑100 (MQ=255)
                                                                                                   |                                                                                                
GTTTTCCTCAATGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAGCGTGACGACCAC  >  NC_000913/4324355‑4324550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: