Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,352,460 T→G 19.5% intergenic (+103/‑124) yjdJ → / → ghoS GNAT family putative N‑acetyltransferase/antitoxin of GhoTS toxin‑antitoxin pair; endonuclease for ghoT mRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,352,4600TG19.5% 255.0 / 60.7 122intergenic (+103/‑124)yjdJ/ghoSGNAT family putative N‑acetyltransferase/antitoxin of GhoTS toxin‑antitoxin pair; endonuclease for ghoT mRNA
Reads supporting (aligned to +/- strand):  ref base T (55/43);  new base G (16/8);  total (71/51)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 129 total aligned reads displayed.

AGAGTACAGAGTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTCCCCAGAGCG  >  NC_000913/4352362‑4352559
                                                                                                  |                                                                                                    
 gagTACAGAGTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGtt                                                                                                     <  1:1275630/99‑1 (MQ=255)
  agTACAGAGTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGtttt                                                                                                   <  1:1359331/100‑1 (MQ=255)
    tACAGAGTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGtttttt                                                                                                 >  2:895817/1‑100 (MQ=255)
    tACAGAGTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGtttttt                                                                                                 <  2:1524781/100‑1 (MQ=255)
      cAGAGTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTg                                                                                               >  1:670991/1‑100 (MQ=255)
       agagTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGt                                                                                              <  2:505723/100‑1 (MQ=255)
        gagTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTc                                                                                             <  2:1700164/100‑1 (MQ=255)
        gagTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTc                                                                                             >  1:228322/1‑100 (MQ=255)
          gTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTccc                                                                                           >  2:1031073/1‑100 (MQ=255)
            cACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCaa                                                                                         >  1:124148/1‑100 (MQ=255)
            cACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCaa                                                                                         >  1:922536/1‑100 (MQ=255)
            cACGATACTTTTCATTCTCTCCACGATGTGAAGAAGGAGAGCATCTGAAGGTTAGGGGGATAGACAAAGAATGGGCAGAGAGCGCGgttttttttttcaa                                                                                         >  2:590502/1‑93 (MQ=255)
             aCGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAg                                                                                        <  2:567780/100‑1 (MQ=255)
             aCGATATTTTTCATTCTCTCCAAGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTTTCCCa                                                                                          >  2:1368159/1‑98 (MQ=255)
              cGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGt                                                                                       >  2:1339306/1‑100 (MQ=255)
               gATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTc                                                                                      <  2:2123693/100‑1 (MQ=255)
                atatTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCa                                                                                     >  1:2340376/1‑100 (MQ=255)
                 tatTTTTCATTCTCTCCGCGATGTGATGCAGGTGCGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGt                                                                                       <  1:1953124/97‑1 (MQ=255)
                  atTTTTCATTCTCTTCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGCTGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATc                                                                                   >  2:2378204/1‑100 (MQ=255)
                   tttttCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATc                                                                                   <  1:1879330/99‑1 (MQ=255)
                    ttttCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATccc                                                                                 >  2:1425070/1‑100 (MQ=255)
                     tttCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATcccc                                                                                <  1:294022/100‑1 (MQ=255)
                       tCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCtt                                                                              <  1:1789744/100‑1 (MQ=255)
                       tCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCtt                                                                              <  1:1735606/100‑1 (MQ=255)
                         aTTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCttt                                                                             <  1:174716/99‑1 (MQ=255)
                          ttCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGGTGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTAc                                                                           >  2:1935555/1‑100 (MQ=255)
                            ctctcCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTg                                                                         >  1:2104441/1‑100 (MQ=255)
                              ctcCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAg                                                                       <  2:2104418/100‑1 (MQ=255)
                              ctcCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAg                                                                       <  2:209396/100‑1 (MQ=255)
                              ctcCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAg                                                                       <  1:243908/100‑1 (MQ=255)
                              ctcCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAg                                                                       <  1:997367/100‑1 (MQ=255)
                              ctcCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAg                                                                       <  2:1289208/100‑1 (MQ=255)
                               tcCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCttt                                                                             >  2:1168936/1‑93 (MQ=255)
                                  gcgATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCaaa                                                                  >  1:627602/1‑100 (MQ=255)
                                   cgATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGGTGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCaaaa                                                                 >  2:484729/1‑100 (MQ=255)
                                      tgtgATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCaaaaaaa                                                              <  1:14498/100‑1 (MQ=255)
                                        tgATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAg                                                            <  1:2337479/100‑1 (MQ=255)
                                        tgATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAg                                                            <  1:943540/100‑1 (MQ=255)
                                         gATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGa                                                           >  2:2345140/1‑100 (MQ=255)
                                           tGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAat                                                         >  1:1584671/1‑100 (MQ=255)
                                           tGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAat                                                         >  1:1584662/1‑100 (MQ=255)
                                            gCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAata                                                        >  2:754105/1‑100 (MQ=255)
                                            gCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCACCTTTACTGAGCAAAAAAAAGAata                                                        >  2:853746/1‑100 (MQ=255)
                                             cAGGAGAGCATCTGAAGAGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTCAGCAAAAAAAAGAatat                                                       >  2:829052/1‑100 (MQ=255)
                                              aGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAat                                                         <  1:92749/97‑1 (MQ=255)
                                              aGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATAtc                                                      >  1:1130335/1‑100 (MQ=255)
                                               ggAGAGCATCTGAAGAGTAGAGGGATACACAAAGAATGGGCAGAGAGCGCGGTCTTTTGTCCCAAGTCATACACTTTACTGAGCAAAAAAAAGAATAtct                                                     >  2:868378/1‑100 (MQ=255)
                                                 agagCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTcc                                                   >  1:727977/1‑100 (MQ=255)
                                                   agCATCTGAAGGGTAGGGGGATGCACAAAGAATCGGCAAAGATCGCGTTTTTTTGTCCCAAGTCATCCTCTAAAAAAAAAAAAAAAAAGAATATCTCCta                                                 <  2:1322229/100‑1 (MQ=255)
                                                    gCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCtat                                                >  2:978892/1‑100 (MQ=255)
                                                     cATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCtata                                               >  2:92981/1‑100 (MQ=255)
                                                     cATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTAATGAGAAAAAAAAAGAATATCTCCtata                                               <  2:993965/100‑1 (MQ=255)
                                                       tCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATg                                             >  2:2242243/1‑100 (MQ=255)
                                                       tCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATg                                             >  2:1183427/1‑100 (MQ=255)
                                                       tCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATg                                             >  2:1887976/1‑100 (MQ=255)
                                                       tCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATg                                             <  1:1721976/100‑1 (MQ=255)
                                                         tGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATtag                                           >  1:101560/1‑100 (MQ=255)
                                                         tGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATgag                                           <  1:255341/100‑1 (MQ=255)
                                                          gAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATgaaa                                          >  2:1141199/1‑98 (MQ=255)
                                                          gAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGAAAAAAAAAGAATATCTCCTATATgaga                                          >  2:827690/1‑100 (MQ=255)
                                                          gAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATgaga                                          >  2:1676534/1‑100 (MQ=255)
                                                            aGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAat                                        >  1:1369108/1‑100 (MQ=255)
                                                             gggTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAatc                                       >  2:2201474/1‑100 (MQ=255)
                                                               gTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAatcat                                     >  2:299163/1‑100 (MQ=255)
                                                                  gggggATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCaa                                  >  1:957914/1‑100 (MQ=255)
                                                                  gggggATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCaa                                  >  1:1543257/1‑100 (MQ=255)
                                                                       aTGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCggg                             <  1:786171/100‑1 (MQ=255)
                                                                       aTGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCggg                             <  2:727969/100‑1 (MQ=255)
                                                                        tGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAAt                                 >  1:867375/1‑95 (MQ=255)
                                                                        tGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCgggg                            >  1:1835261/1‑100 (MQ=255)
                                                                         tcacaAAGAATGGGCAGAAAGCACATTCTATTTTCCCAAGTTATTACCTTTGCTGAGCAAAAAAAATAATATGTAATATATGAAAATAATAAATCgggg                            >  2:1016970/2‑99 (MQ=255)
                                                                         gCACAAAGAATGGGCAGAGAGCTCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCgggg                            <  2:1355468/99‑1 (MQ=255)
                                                                         gCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGt                           >  1:816016/1‑100 (MQ=255)
                                                                         gCACAAAGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGGATATCTCCTATATGAGAATCATCAATCGGGGt                           <  2:1876356/100‑1 (MQ=255)
                                                                          cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAGAAAAGACTATCTCCTATATGAGAATCATCAATCGGGGtt                          >  2:1686444/1‑100 (MQ=255)
                                                                          cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCACCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGtt                          <  2:853466/100‑1 (MQ=255)
                                                                             aaaGAATGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCACTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGtt                          <  2:922102/97‑1 (MQ=255)
                                                                              aaGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTtaata                      >  1:1353483/1‑100 (MQ=255)
                                                                               aGAATGGTGAGAGAGGTTGTTTTTTTTTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTTTTATATGATATTCTTCAATCGGGGTtaataa                     <  2:384181/100‑1 (MQ=255)
                                                                                   tGGGCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGttt                 >  1:1006090/1‑100 (MQ=255)
                                                                                    gggCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGtttt                >  1:1192825/1‑100 (MQ=255)
                                                                                     ggCAGAGAGTGCGTTTTTTTGTCCCAAGTTATCCCCTAAAAAAAAAAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGtttt                <  2:638720/99‑1 (MQ=255)
                                                                                     ggCAGAGAGCGCGTGTTTTTGTCCCAAGTCAGCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTg               <  2:2082535/100‑1 (MQ=255)
                                                                                     ggCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGtttt                >  2:615236/1‑99 (MQ=255)
                                                                                     ggCAGAGAGCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTg               >  1:1768928/1‑100 (MQ=255)
                                                                                       cAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCg             <  1:1980966/100‑1 (MQ=255)
                                                                                       cAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCg             <  1:881166/100‑1 (MQ=255)
                                                                                        agatagCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTg               <  1:1811368/98‑1 (MQ=255)
                                                                                         gagagCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTc           >  1:1822301/1‑100 (MQ=255)
                                                                                         gagagCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTc           >  1:793397/1‑100 (MQ=255)
                                                                                          agagCGCGGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTcc          <  1:652325/100‑1 (MQ=255)
                                                                                           gagCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTccc         >  1:1494069/1‑100 (MQ=255)
                                                                                            agCGCGTTTTTTTGTCCCAAGTCATCACCTTTACTGATCAAAAAAAAGAATATCTCCTATATTAGAATCATCAATCGGGGTTAATAAGTTTTGCGTcccc        <  2:535564/100‑1 (MQ=255)
                                                                                             gcgcGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTCCCCa       >  2:742696/1‑100 (MQ=255)
                                                                                             gcgcGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTCCCCa       >  1:528080/1‑100 (MQ=255)
                                                                                              cgcgTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTCCCCag      >  2:360686/1‑100 (MQ=255)
                                                                                               gcgGTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTCCCCaga     >  1:1465324/1‑100 (MQ=255)
                                                                                                cgTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTCCCCagag    >  1:116643/1‑100 (MQ=255)
                                                                                                cgTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTCACCagag    >  2:1092070/1‑100 (MQ=255)
                                                                                                  tttttttGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTCCCCAGAGCg  <  1:2295732/100‑1 (MQ=255)
                                                                                                  |                                                                                                    
AGAGTACAGAGTCACGATATTTTTCATTCTCTCCGCGATGTGATGCAGGAGAGCATCTGAAGGGTAGGGGGATGCACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGCGTCCCCAGAGCG  >  NC_000913/4352362‑4352559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: