Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 428,188 G→T 100% R181L (CGC→CTC)  secD → SecYEG protein translocase auxillary subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913428,1880GT98.2% 262.5 / ‑3.8 79R181L (CGC→CTC) secDSecYEG protein translocase auxillary subunit
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (38/38);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGC  >  NC_000913/428093‑428281
                                                                                               |                                                                                             
aaaTATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGAt                                                                                           <  2:1700636/100‑1 (MQ=255)
 attatCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGAt                                                                                           <  1:2125728/97‑1 (MQ=255)
  atatCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGc                                                                                         <  1:94501/100‑1 (MQ=255)
  atatCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGc                                                                                         <  1:1540022/100‑1 (MQ=255)
       gATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAg                                                                                    >  1:2339958/1‑100 (MQ=255)
       gATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAg                                                                                    <  1:1316122/100‑1 (MQ=255)
        aTAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGc                                                                                   <  1:2038711/100‑1 (MQ=255)
            ccTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGt                                                                               <  2:200166/100‑1 (MQ=255)
             cTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtg                                                                              <  2:779995/100‑1 (MQ=255)
              tACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtga                                                                             >  1:1583850/1‑100 (MQ=255)
               aCGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtgat                                                                            <  2:2131125/100‑1 (MQ=255)
                 gCAGTGACCTGCGCGAAAAGGGCATCCCATATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtgatga                                                                          >  1:1883635/1‑100 (MQ=255)
                  cAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGaa                                                                         >  1:1774195/1‑100 (MQ=255)
                   aGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAg                                                                        <  2:1656010/100‑1 (MQ=255)
                   aGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAg                                                                        <  2:759724/100‑1 (MQ=255)
                    gTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGc                                                                       <  1:1870665/100‑1 (MQ=255)
                     tGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGcc                                                                      >  2:300177/1‑100 (MQ=255)
                      gACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCa                                                                     >  1:2115165/1‑100 (MQ=255)
                        ccTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCAtt                                                                   >  1:210793/1‑100 (MQ=255)
                         cTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTg                                                                  <  1:1219388/100‑1 (MQ=255)
                         cTGCGCAAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTg                                                                  <  2:1679314/100‑1 (MQ=255)
                          tGCGCGAGTAGGGCATCCCTTATACTACTTTACGTTAAGAAAATAATTACGGCCTGAGCATCACTTTCCTCTATGCTAAAGCTCGTGATGAAGc                                                                       <  2:2164450/94‑1 (MQ=255)
                           gcgcGAAAAGGGCATCTCGTATACCACTGTTCGTAAAGAATACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGc                                                                 <  2:2218554/99‑1 (MQ=255)
                            cgcgAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGt                                                               >  1:384675/1‑100 (MQ=255)
                            cgcgAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGt                                                               >  2:1242182/1‑100 (MQ=255)
                              cgAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATGACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTAt                                                             >  2:1688762/1‑100 (MQ=255)
                              cgAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTa                                                              <  1:1966231/99‑1 (MQ=255)
                              cgAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTAt                                                             >  1:1888038/1‑100 (MQ=255)
                              cgAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTAt                                                             >  2:1872696/1‑100 (MQ=255)
                               gAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATc                                                            <  1:1175584/100‑1 (MQ=255)
                                aaaaGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCt                                                           >  2:1840524/1‑100 (MQ=255)
                                 aaaGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTg                                                          <  1:2091383/100‑1 (MQ=255)
                                 aaaGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTg                                                          <  2:193678/100‑1 (MQ=255)
                                   aGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAg                                                        <  2:450159/100‑1 (MQ=255)
                                     ggCATCCCGTATACCACTGTTCGTAAAGAAAAGAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCa                                                      <  1:1010394/100‑1 (MQ=255)
                                     ggCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCa                                                      <  1:531534/100‑1 (MQ=255)
                                          cccGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAgcgc                                                 >  2:16010/1‑100 (MQ=255)
                                           ccGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGcc                                                >  2:1192696/1‑100 (MQ=255)
                                           ccGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGcc                                                >  2:1342434/1‑100 (MQ=255)
                                                taCCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCg                                           >  1:1636071/1‑100 (MQ=255)
                                                  ccACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGa                                         >  1:44407/1‑100 (MQ=255)
                                                  ccACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGa                                         <  2:1070753/100‑1 (MQ=255)
                                                  ccACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGa                                         >  1:1201326/1‑100 (MQ=255)
                                                    aCTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGAcc                                       <  2:1542550/100‑1 (MQ=255)
                                                          cGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGa                                 >  1:195226/1‑100 (MQ=255)
                                                          cGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGa                                 <  1:1215471/100‑1 (MQ=255)
                                                           gTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAt                                >  2:895996/1‑100 (MQ=255)
                                                           gTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAt                                >  2:1643280/1‑100 (MQ=255)
                                                           gTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAt                                >  2:1642952/1‑100 (MQ=255)
                                                            tAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAtt                               <  2:992099/100‑1 (MQ=255)
                                                            tAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAtt                               >  1:801983/1‑100 (MQ=255)
                                                            tAAAGAAAACAACTACGGCCTGAGCATCACTTTAAACGATGATAAAGATCGTGAGGAAGCCATTGCGTATATGAGCAAGCGCCATCCGGACCTGGTGa                                 >  2:752119/1‑98 (MQ=255)
                                                              aaGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGGCATGGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTag                             >  1:1191465/1‑100 (MQ=255)
                                                               aGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTagc                            >  2:481751/1‑100 (MQ=255)
                                                               aGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTag                             <  2:1038497/99‑1 (MQ=255)
                                                                gAAAACAACTACGGCCTGAGCATCACTTTCCTCGATACTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTagca                           >  2:1509745/1‑100 (MQ=255)
                                                                 aaaaCAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTagcag                          >  1:42985/1‑100 (MQ=255)
                                                                   aacaacTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGcc                        <  2:1647970/100‑1 (MQ=255)
                                                                      aacTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAgg                     <  1:1866683/100‑1 (MQ=255)
                                                                       acTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAggg                    <  1:1342448/100‑1 (MQ=255)
                                                                        cTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGgc                   <  2:1774174/100‑1 (MQ=255)
                                                                          aCGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGgca                  >  1:356767/1‑99 (MQ=255)
                                                                           cGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGgcagc                <  2:1632844/100‑1 (MQ=255)
                                                                             gCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCaa              >  1:1470651/1‑100 (MQ=255)
                                                                               cttccgatctcTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAAcc            <  2:2244040/90‑1 (MQ=255)
                                                                               cTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAAcc            <  1:1726980/100‑1 (MQ=255)
                                                                               cTGAGCATCACTTTCCGCGATGCTAAAGCTCGTAATGAAGCAATTGCGTATCTGAGCAAGCTCCATCCGGAACTGGTGATTAGCAGCCAGGGCAGCAAcc            >  2:483920/1‑100 (MQ=255)
                                                                                tGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCTAGGGCAGCAACCa           <  1:1630054/100‑1 (MQ=255)
                                                                                tGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCa           >  2:1779611/1‑100 (MQ=255)
                                                                                  aGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGc         >  1:892570/1‑100 (MQ=255)
                                                                                  aGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGc         <  2:1538798/100‑1 (MQ=255)
                                                                                   gCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCt        >  1:1724839/1‑100 (MQ=255)
                                                                                   gCATAACTTTCCTCGATGCTAAAGCTCGTCATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGc         <  1:1914584/99‑1 (MQ=255)
                                                                                      tCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcg     >  1:55479/1‑100 (MQ=255)
                                                                                      tCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTAATTAGCAGCCAGGGCAGCAACCAGAtgcg     >  2:789488/1‑100 (MQ=255)
                                                                                        aCTTTCCTCGATGCTAAAGCTCGTGATGAAGTCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcgtg   <  2:2080281/100‑1 (MQ=255)
                                                                                        aCTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcgtg   >  2:1738250/1‑100 (MQ=255)
                                                                                        aCTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcgtg   >  2:279249/1‑100 (MQ=255)
                                                                                         cTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGGGATTAGCAGCCAGGGCAGCAACCAgatcggaag  >  1:2244066/1‑92 (MQ=255)
                                                                                               |                                                                                             
AAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGC  >  NC_000913/428093‑428281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑