Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,434,437 T→A 40.8% W109R (TGG→AGG)  ytfH → DUF24 family HxlR‑type putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,434,4370TA40.8% 68.6 / 121.5 103W109R (TGG→AGG) ytfHDUF24 family HxlR‑type putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (31/30);  new base A (17/25);  total (48/55)
Fisher's exact test for biased strand distribution p-value = 3.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01

Only 100 of 103 total aligned reads displayed.

AACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGC  >  NC_000913/4434341‑4434534
                                                                                                |                                                                                                 
aaCCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCCGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGa                                                                                                >  2:1524847/1‑100 (MQ=255)
aaCCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGa                                                                                                >  1:2160744/1‑100 (MQ=255)
 aCCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGAt                                                                                               <  2:1629183/100‑1 (MQ=255)
  ccGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGAtt                                                                                              <  1:1297484/100‑1 (MQ=255)
   cGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTg                                                                                             <  2:471966/100‑1 (MQ=255)
   cGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTAACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTg                                                                                             <  2:450574/100‑1 (MQ=255)
   cGTATCGCGTATCCGGTGGTGAAGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGAAAGGATTg                                                                                             >  2:472543/1‑100 (MQ=255)
     tATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAg                                                                                           <  1:515241/100‑1 (MQ=255)
     tATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAg                                                                                           <  1:1054814/100‑1 (MQ=255)
      aTCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGt                                                                                          <  2:685194/100‑1 (MQ=255)
        cgcgTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTg                                                                                        <  1:503076/100‑1 (MQ=255)
         gcgTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGa                                                                                       >  2:2139197/1‑100 (MQ=255)
           gTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAAt                                                                                     <  2:2168815/100‑1 (MQ=255)
            tATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAAtt                                                                                    >  2:1770176/1‑100 (MQ=255)
            tATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAAtt                                                                                    <  1:522407/100‑1 (MQ=255)
            tATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAAtt                                                                                    <  1:1645722/100‑1 (MQ=255)
            tATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAAtt                                                                                    <  1:2118446/100‑1 (MQ=255)
             aTCCGGTGGTGCCGCCGCATGTTGAATATAGCTTCACGCCGCTGGGCGAGCAGGTGAGCTAATATGTTTCTTCACTGTTTGACTGGATTGAGTTGAAttt                                                                                   <  2:1049113/100‑1 (MQ=255)
             aTCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAAttt                                                                                   >  1:374892/1‑100 (MQ=255)
              tCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTg                                                                                  <  2:2237936/100‑1 (MQ=255)
               ccGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGc                                                                                 >  2:1849885/1‑100 (MQ=255)
                nggtggtGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGcc                                                                                >  1:1915316/2‑100 (MQ=255)
                cGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAACAGGTTGCTGCCCTGGCGGACTGCATTGAGTTGAATTTGcc                                                                                >  1:1107041/1‑100 (MQ=255)
                cGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGcc                                                                                <  1:1499794/100‑1 (MQ=255)
                cGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGCTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGcc                                                                                <  2:1283024/100‑1 (MQ=255)
                    ggtgCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAg                                                                            >  1:639136/1‑100 (MQ=255)
                    ggtgCCACCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAg                                                                            >  2:1568021/1‑100 (MQ=255)
                         cgccgcATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGtt                                                                       >  1:1262999/1‑100 (MQ=255)
                         cgccgcATGTGAAGTATAGTCTCACGCCGCTCGGCCAGCAGGTGAGCGAAAAGGTTGCTCCACTGCCTGACTGGATTCAGTTGact                                                                                     <  2:2228248/86‑3 (MQ=255)
                          gccgcATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTg                                                                      >  1:373996/1‑100 (MQ=255)
                          gccgcATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTAtt                                                                       >  1:64631/1‑99 (MQ=255)
                          gccgcATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTg                                                                      >  1:1502663/1‑100 (MQ=255)
                          gccgcATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTg                                                                      <  2:1390788/100‑1 (MQ=255)
                           ccgcATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTgg                                                                     <  1:2012239/100‑1 (MQ=255)
                            cgcATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGc                                                                    >  1:1697000/1‑100 (MQ=255)
                            cgcATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGc                                                                    >  2:331147/1‑100 (MQ=255)
                            cgcATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGc                                                                    <  1:529963/100‑1 (MQ=255)
                              cATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCgg                                                                  <  2:1096482/100‑1 (MQ=255)
                               aTGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGATTTGAATTTGTCCGAGGTGTTTGCgt                                                                  <  2:331003/99‑2 (MQ=255)
                               aTGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTg                                                                <  2:734914/100‑1 (MQ=255)
                                tgtgGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTATTAGCGGt                                                                 >  1:692729/1‑99 (MQ=255)
                                 gtgGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGc                                                               >  2:516619/1‑100 (MQ=255)
                                 gtgGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGc                                                               >  2:689229/1‑100 (MQ=255)
                                  tgGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCg                                                              >  1:1144588/1‑100 (MQ=255)
                                   ggAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAACGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCgg                                                             >  1:896583/1‑100 (MQ=255)
                                   ggAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCgg                                                             >  2:387734/1‑100 (MQ=255)
                                   ggAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCgg                                                             <  1:1700376/100‑1 (MQ=255)
                                    gAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCggg                                                            <  2:1865557/100‑1 (MQ=255)
                                     aaTATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCggg                                                            <  2:2104709/99‑1 (MQ=255)
                                     aaTATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGa                                                           <  2:417276/100‑1 (MQ=255)
                                      atatAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGAt                                                          <  1:910519/100‑1 (MQ=255)
                                      agataGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGAt                                                          <  2:2298608/98‑1 (MQ=255)
                                        ataGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGAGTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGa                                                        <  2:2260950/100‑1 (MQ=255)
                                        ataGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGa                                                        <  2:1922228/100‑1 (MQ=255)
                                           gCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACg                                                     >  2:467643/1‑100 (MQ=255)
                                           gCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACg                                                     >  1:182564/1‑100 (MQ=255)
                                           gCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACg                                                     <  2:317205/100‑1 (MQ=255)
                                               cACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGTTTGAGTTGAATTTGCCCTATGTGTTGGTGGTGCGGGATGAACGTGc                                                  <  2:2134237/99‑1 (MQ=255)
                                                aCGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCg                                                 >  1:58744/1‑99 (MQ=255)
                                                 cgccgcTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGGACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGc                                               <  2:800996/100‑1 (MQ=255)
                                                   ccgcTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCAt                                             >  1:27617/1‑100 (MQ=255)
                                                   ccgcTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCAt                                             >  2:846951/1‑100 (MQ=255)
                                                   ccgcTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCAt                                             >  2:847387/1‑100 (MQ=255)
                                                   ccgcTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTGGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCAt                                             <  1:1928707/100‑1 (MQ=255)
                                                    cgcTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATa                                            <  2:799854/100‑1 (MQ=255)
                                                    cgcTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATa                                            >  1:1024137/1‑100 (MQ=255)
                                                     gcTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGATGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATaa                                           >  2:1784409/1‑100 (MQ=255)
                                                       tGGGCGAGCAGGTGAGCGAAAAGGTAGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAATGTGCGGCATAAc                                          <  2:257698/99‑1 (MQ=255)
                                                          gCGAGCAGGTGAGCGAAAAGGTTGATGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGTGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGc                                       <  2:2027111/99‑1 (MQ=255)
                                                           cGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCg                                     <  1:1942876/100‑1 (MQ=255)
                                                           cGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCg                                     >  1:630702/1‑100 (MQ=255)
                                                               cAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATg                                 <  1:1352475/100‑1 (MQ=255)
                                                               cAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATg                                 >  2:1629626/1‑100 (MQ=255)
                                                                aGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATgc                                >  2:2007994/1‑100 (MQ=255)
                                                                aGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATgc                                <  2:391717/100‑1 (MQ=255)
                                                                 ggTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATgcg                               >  1:2121941/1‑100 (MQ=255)
                                                                 ggTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATgcg                               >  2:765232/1‑100 (MQ=255)
                                                                 ggTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATgcg                               >  2:1120628/1‑100 (MQ=255)
                                                                  gTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATgcgc                              <  1:1656941/100‑1 (MQ=255)
                                                                   tGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCt                             <  1:172345/100‑1 (MQ=255)
                                                                      gCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAg                          >  1:229066/1‑100 (MQ=255)
                                                                       cGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGc                         >  1:249/1‑100 (MQ=255)
                                                                        gAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCt                        <  2:1271662/100‑1 (MQ=255)
                                                                        gAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCt                        >  1:194004/1‑100 (MQ=255)
                                                                        gAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCt                        <  1:1731565/100‑1 (MQ=255)
                                                                             ggTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCt                   >  1:1935473/1‑100 (MQ=255)
                                                                              gTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTg                  <  2:2076337/100‑1 (MQ=255)
                                                                               ttGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTgg                 <  1:994677/100‑1 (MQ=255)
                                                                                 gctgcACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTc               >  2:2322981/1‑100 (MQ=255)
                                                                                 gctgcACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTc               <  1:1943248/100‑1 (MQ=255)
                                                                                  ctgcACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCt              >  2:115077/1‑100 (MQ=255)
                                                                                    gcACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCTGGATGCGCTGAGCTTATCTGGTCTAc            >  1:712341/1‑100 (MQ=255)
                                                                                      aCTGGCGGACAGGATTGAGTTGAATTTTCCCGAGGTGTTGGCGGTGCGTGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACgg          <  2:1382737/100‑1 (MQ=255)
                                                                                      aCTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACgg          <  1:2156296/100‑1 (MQ=255)
                                                                                        tGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAGCGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTg        <  1:295343/100‑1 (MQ=255)
                                                                                         ggCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGc       <  1:1706134/100‑1 (MQ=255)
                                                                                         ggCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGc       >  1:1183684/1‑100 (MQ=255)
                                                                                            ggACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATg    >  1:504920/1‑100 (MQ=255)
                                                                                              aCTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGc  <  1:2309416/100‑1 (MQ=255)
                                                                                              aCTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGc  <  2:1584726/100‑1 (MQ=255)
                                                                                                |                                                                                                 
AACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCATGGC  >  NC_000913/4434341‑4434534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: