Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,560,045 C→A 51.0% W212C (TGG→TGT yjiH ← nucleoside recognition pore and gate family putative inner membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,560,0450CA51.0% ‑5.5 / 148.9 99W212C (TGG→TGTyjiHnucleoside recognition pore and gate family putative inner membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (26/22);  new base A (25/25);  total (51/48)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

GCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCG  >  NC_000913/4559947‑4560144
                                                                                                  |                                                                                                   
gcgcgcgcCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGcc                                                                                                    <  2:2205186/100‑1 (MQ=255)
gcgcgcgcCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGcc                                                                                                    <  1:771486/100‑1 (MQ=255)
gcgcgcgcCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGcc                                                                                                    <  1:1361625/100‑1 (MQ=255)
 cgcgcgcCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACa                                                                                                   <  1:2218120/100‑1 (MQ=255)
  gcgcgcCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCa                                                                                                   <  1:415310/99‑1 (MQ=255)
       ccATCGATAAACATATCCATGACGTTTTTGCTTACTTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACATAcgcg                                                                                             >  2:381741/1‑100 (MQ=255)
       ccATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGAcgcg                                                                                             <  2:1168543/100‑1 (MQ=255)
          tCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCaa                                                                                          >  1:1360962/1‑100 (MQ=255)
           cGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCaaa                                                                                         >  1:328049/1‑100 (MQ=255)
            gATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCaaaa                                                                                        >  1:1186709/1‑100 (MQ=255)
               aaaCATATCCATTACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGt                                                                                     <  1:1181302/100‑1 (MQ=255)
               aaaCATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGt                                                                                     <  1:533611/100‑1 (MQ=255)
                aaCATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGtt                                                                                    >  1:155883/1‑100 (MQ=255)
                aaCATATCCATGACGTATTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTTGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAACATGtt                                                                                    >  2:1098675/1‑100 (MQ=255)
                 aCATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTg                                                                                   <  1:2341758/100‑1 (MQ=255)
                 aCATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTg                                                                                   <  2:346263/100‑1 (MQ=255)
                  cATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTgg                                                                                  >  2:1710079/1‑100 (MQ=255)
                  cATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTgg                                                                                  >  2:456118/1‑100 (MQ=255)
                    tatCCATGACGTTTTTGCGGACCTGAGTTGTCATGTTTGCGCTCCTTGCGTTGGGTTGATACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGttt                                                                                   <  2:1393472/97‑2 (MQ=255)
                    tatCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCt                                                                                <  2:124520/100‑1 (MQ=255)
                      tCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTc                                                                               <  1:845256/99‑1 (MQ=255)
                      tCCATGACGTTTTTGCAGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGAGGTTCTTCGAAGTTACGCCAGACGCGCAAAATGTTGGCTc                                                                               >  2:2087004/1‑99 (MQ=255)
                       ccATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCg                                                                             >  2:1198647/1‑100 (MQ=255)
                       ccATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCg                                                                             <  2:1552042/100‑1 (MQ=255)
                        cATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGa                                                                            <  2:304650/100‑1 (MQ=255)
                         aTGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGAc                                                                           >  1:1966927/1‑100 (MQ=255)
                                ttttGCCGATCTGGGTTGTCATGCTTGCGTTGCTTGCGGGGGGGTGCGTCGTTCTTCGAAGTTATGTCAGGAGCGGATATTGTTGGCTCCGACATAttt                                                                     <  2:2180657/99‑1 (MQ=255)
                                   tGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGCCGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCgccca                                                                         >  2:1380066/1‑88 (MQ=255)
                                     cgtaCCTGAGTTGTCATGCTTGAGCTCCTTGAGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAaca                                                                >  2:2066842/4‑99 (MQ=255)
                                     cGGACCTGAGTTGTCATGCTTGCGCTACTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAacac                                                               >  2:703049/1‑100 (MQ=255)
                                           tGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGa                                                          >  2:1594732/1‑99 (MQ=255)
                                           tGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGAt                                                         >  2:1106916/1‑100 (MQ=255)
                                           tGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGAt                                                         >  2:1160468/1‑100 (MQ=255)
                                              gTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGAc                                                      >  1:1303412/1‑100 (MQ=255)
                                              gTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGATCCGACAAATTTAAACACCAGGATGAc                                                      >  1:156831/1‑100 (MQ=255)
                                                tGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACgg                                                    >  1:2389955/1‑100 (MQ=255)
                                                tGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACgg                                                    <  1:484760/100‑1 (MQ=255)
                                                tGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACgg                                                    >  1:1488318/1‑100 (MQ=255)
                                                 gTCATGCTTGCGCTTCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGc                                                   <  2:1425811/100‑1 (MQ=255)
                                                 gTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACgg                                                    >  2:1493540/1‑99 (MQ=255)
                                                 gTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGc                                                   <  2:1530200/100‑1 (MQ=255)
                                                 gTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGc                                                   >  1:2344921/1‑100 (MQ=255)
                                                 gTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGc                                                   <  2:1168050/100‑1 (MQ=255)
                                                   cATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACAGCAGGATGACGGCTa                                                 >  2:631337/1‑100 (MQ=255)
                                                   cATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTa                                                 >  1:1593701/1‑100 (MQ=255)
                                                   cATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTa                                                 >  2:823813/1‑100 (MQ=255)
                                                     tGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGACGTTAAGCCAGACGCGCCAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTaaa                                               >  2:2134594/1‑100 (MQ=255)
                                                     tGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTaaa                                               <  2:1966905/100‑1 (MQ=255)
                                                     tGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTaaa                                               >  2:1967501/1‑100 (MQ=255)
                                                     tGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTaaa                                               >  2:2357877/1‑100 (MQ=255)
                                                     tGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTaaa                                               >  1:2374562/1‑100 (MQ=255)
                                                      gCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAg                                              <  1:234908/100‑1 (MQ=255)
                                                       cTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAgg                                             <  1:498926/100‑1 (MQ=255)
                                                        ttGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGt                                            <  2:304921/100‑1 (MQ=255)
                                                         tGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTa                                           >  2:869694/1‑100 (MQ=255)
                                                         tGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTa                                           <  1:9630/100‑1 (MQ=255)
                                                          gcgcTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTAc                                          <  2:1992921/100‑1 (MQ=255)
                                                          gcgcTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTAc                                          >  1:442453/1‑100 (MQ=255)
                                                           cgcTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGATAAAGGTACg                                         <  2:1040899/100‑1 (MQ=255)
                                                           cgcTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAAGGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACg                                         <  1:2304486/100‑1 (MQ=255)
                                                            ggtagatGCGGTGAGTTGCGACGTTGTTCGAAGTTATGACAAACGCGTAAAATGTTGGCTCCGATAAATTTTAACGCTTGGATGACGGCTAGAgg                                             <  2:1696185/89‑1 (MQ=255)
                                                             cTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGAt                                       <  2:123378/100‑1 (MQ=255)
                                                             cTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGAt                                       >  1:2227918/1‑100 (MQ=255)
                                                              tCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGAtt                                      <  2:56337/100‑1 (MQ=255)
                                                              tCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGAtt                                      <  2:432229/100‑1 (MQ=255)
                                                                cTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACTCCAGGATTACGGCTAAAGGTACGAt                                       <  2:212773/97‑1 (MQ=255)
                                                                 ttGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACg                                   <  1:863685/100‑1 (MQ=255)
                                                                 ttGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACg                                   <  2:1057307/100‑1 (MQ=255)
                                                                 ttGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACg                                   <  2:1298719/100‑1 (MQ=255)
                                                                   gCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGa                                 >  1:1563901/1‑100 (MQ=255)
                                                                   gCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACgg                                  <  1:1160479/99‑1 (MQ=255)
                                                                     gTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACg                               >  1:609195/1‑100 (MQ=255)
                                                                     gTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACg                               <  1:958036/100‑1 (MQ=255)
                                                                     gTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACg                               <  2:1723744/100‑1 (MQ=255)
                                                                        gggTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGc                            <  1:1309516/100‑1 (MQ=255)
                                                                          gTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGccc                          <  2:1858814/100‑1 (MQ=255)
                                                                          gTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGccc                          <  1:543757/100‑1 (MQ=255)
                                                                          gTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGccc                          <  2:1001097/100‑1 (MQ=255)
                                                                             gCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGa                       >  2:1601958/1‑100 (MQ=255)
                                                                              cGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGaa                      >  2:2325732/1‑100 (MQ=255)
                                                                                  gTTTTTCGTAGTTAAGACAGACGCGCAATATGTTGGCTCCGACAAATTTAGACACCAGTATGACGGCGAAAGGTACGTTTATTGACATGCCCAGAAAGGc                  <  2:65187/100‑1 (MQ=255)
                                                                                  gTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGc                  >  2:1797381/1‑100 (MQ=255)
                                                                                  gTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGc                  <  2:568856/100‑1 (MQ=255)
                                                                                    tcttcGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCaa                <  2:2304126/100‑1 (MQ=255)
                                                                                     cttcGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCaaa               >  2:2114193/1‑100 (MQ=255)
                                                                                      ttcGCAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGATAAAGGTACGATTACGGACGTGACCAGAAAGGCAAac              >  2:226103/1‑100 (MQ=255)
                                                                                      ttcGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAac              >  1:1073896/1‑100 (MQ=255)
                                                                                      ttcGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAac              >  1:2292244/1‑100 (MQ=255)
                                                                                       tcGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAaca             >  2:1855227/1‑100 (MQ=255)
                                                                                       tcGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAaca             >  1:158518/1‑100 (MQ=255)
                                                                                         gAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACAcc           >  2:1093671/1‑100 (MQ=255)
                                                                                          aaGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACcg          >  2:748420/1‑100 (MQ=255)
                                                                                           aGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACcgc         <  2:1881671/100‑1 (MQ=255)
                                                                                            gTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACcgcg        <  2:1051999/100‑1 (MQ=255)
                                                                                            gTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACcgcg        <  1:1766632/100‑1 (MQ=255)
                                                                                             ttAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGa       >  2:920722/1‑100 (MQ=255)
                                                                                              tAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGa       >  2:1715954/1‑99 (MQ=255)
                                                                                               aaGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACg     >  2:1553211/1‑100 (MQ=255)
                                                                                               aaGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAACGCAAACACCGCGACg     >  1:575322/1‑100 (MQ=255)
                                                                                                  ccAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCg  >  2:2330407/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
GCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACGCCG  >  NC_000913/4559947‑4560144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: